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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 10,311 results for: otos

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Showing: 9871 - 9900 of 10311 results

  1. pCAGGS_m-AKAR5

    Plasmid
    #114139
    Purpose
    Mammalian expression of the AKAR5 sensor C-terminally fused to the C-terminus of KRas (membrane targeting) for detecting PKA activity using two-photon fluorescence lifetime imaging microscopy (2pFLIM)
    Depositor
    Haining Zhong
    Article
    Tillo et al Cell Rep. 2017 Apr 18;19(3):617-629. doi
    Insert
    AKAR5 C-terminally tagged by KRas C tail
    Expression
    Mammalian
    Available Since
    Oct. 4, 2018
    Availability
    Academic Institutions and Nonprofits only
    114139-plasmid-map-sequence-id-215468

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  2. pCAGGS_cyt-AKAR5

    Plasmid
    #114138
    Purpose
    Mammalian expression of the AKAR5 sensor tagged with nucleus export signal (NES) for detecting PKA activity using two-photon fluorescence lifetime imaging microscopy (2pFLIM)
    Depositor
    Haining Zhong
    Article
    Tillo et al Cell Rep. 2017 Apr 18;19(3):617-629. doi
    Insert
    AKAR5 with C-terminal nuclear export signal (NES)
    Expression
    Mammalian
    Available Since
    Oct. 4, 2018
    Availability
    Academic Institutions and Nonprofits only
    114138-plasmid-map-sequence-id-215467

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  3. KG#84

    Plasmid
    #110878
    Purpose
    Expresses the C. elegans acy--1 P260S gain-of-function cDNA in ventral cord cholinergic motor neurons
    Depositor
    Kenneth Miller
    Article
    Schade et al Genetics. 2005 Feb;169(2):631-49. doi: 1
    Inserts
    unc-17beta promoter
    acy-1 P260S gain-of-function cDNA
    unc-54 3' control region with 1 artificial intron just upstream and 1 artificial intron in control region
    Expression
    Bacterial
    Mutation
    Changed Proline to Serine at amino acid 260
    Available Since
    Aug. 8, 2018
    Availability
    Academic Institutions and Nonprofits only
    110878-plasmid-map-sequence-id-217951

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  5. pPB-Ins-U6p-sgRNAentry-EF1Ap-TetOn3G-IRES-Neo

    Plasmid
    #183411
    Purpose
    piggyBAC-based sgRNA entry (Esp3I) and Tet-On 3G transactivator expression vector. Insert protospacer and transfect with CRISPRa (183409) or CRISPRi (183410) plasmid for inducible epigenome editing.
    Depositor
    Azim Surani
    Article
    Tang et al Nat Cell Biol. 2022 Apr;24(4):448-460. d
    Insert
    Tet-on 3G
    Use
    CRISPR and Synthetic Biology; Piggybac
    Expression
    Mammalian
    Promoter
    EF1A
    Available Since
    May 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    183411-plasmid-map-sequence-id-361257

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  8. TFORF2927

    Plasmid
    #143678
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    NFIX (NFIX Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    143678-plasmid-map-sequence-id-338757

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  10. pH-nCas9-PPE-V2

    Plasmid
    #170131
    Purpose
    For plant prime editing in rice plants or monocotyledons protoplasts
    Depositor
    Caixia Gao
    Article
    Lin et al Nat Biotechnol. 2021 Mar 25. pii: 10.103
    Insert
    nCas9(H840A)-M-MLV
    Use
    CRISPR
    Expression
    Plant
    Mutation
    H840A for Cas9; D200N, T306K, W313F, T330P and L…
    Promoter
    maize Ubiquitin-1, OsU3
    Available Since
    May 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    170131-plasmid-map-sequence-id-333607

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  13. pAS_4xMma PylT_FLAG-PcKRS

    Plasmid
    #140022
    Purpose
    Plasmid with 4xMmaPylT cassette and MmaPylRS for amber suppression and incorporation of photocaged-lysine; for transient or stable piggyBac-mediated integration
    Depositor
    Simon Elsaesser
    Article
    Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006
    Insert
    PcKRS (pylS Methanosarcina bakeri)
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    M241F, A267S, Y271C, L274M
    Promoter
    EF1
    Available Since
    May 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    140022-plasmid-map-sequence-id-297729

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  15. pAAV-Hb9-LMO3 (sbGLuc-VChR1-EYFP)

    Plasmid
    #114103
    Purpose
    fusion protein of Gaussia luciferase variant slow burn, Volvox channelrhodopsin-1, and enhanced yellow fluorescent protein (luminopsin LMO3) for bioluminescent optogenetics
    Depositor
    Ute Hochgeschwender
    Article
    Petersen et al Front Neurol. 2022 Jan 20;12:792643. doi
    Insert
    sbGLuc-VChR1-EYFP
    Use
    AAV
    Expression
    Mammalian
    Promoter
    motoneuron-specific Homeobox 9 promoter
    Available Since
    Aug. 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    114103-plasmid-map-sequence-id-252555

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  16. pH-ABE8e-spG

    Plasmid
    #200775
    Purpose
    For plant adenine base editing in rice plants or monocotyledons protoplasts
    Depositor
    Caixia Gao
    Article
    Xue et al Nat Biotechnol. 2023 Mar 9. doi: 10.1038
    Insert
    nCas9-spG(D10A)-TadA8e
    Use
    CRISPR
    Expression
    Plant
    Mutation
    D10A, D1135L, S1136W, G1218K, E1219Q, R1335Q, T13…
    Promoter
    maize Ubiquitin-1, OsU3
    Available Since
    July 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    200775-plasmid-map-sequence-id-399599

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  19. CMV-CASANOVA

    Plasmid
    #113035
    Purpose
    CASANOVA (AcrIIA4-LOV2 hybrid) expression in mammalian cells; enables optogenetic control of SpyCas9
    Depositor
    Dominik Niopek
    Article
    Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s
    Insert
    CASANOVA (for CRISPR/Cas9 activity switching via a novel optogenetic variant of AcrIIA4)
    Use
    CRISPR and Synthetic Biology
    Expression
    Mammalian
    Available Since
    Oct. 31, 2018
    Availability
    Academic Institutions and Nonprofits only
    113035-plasmid-map-sequence-id-224728

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  23. pTarget

    Plasmid
    #214052
    Purpose
    Expresses target sequence for Haliangium type III CRISPR-Cas complex; identical to pNon-target (Addgene plasmid # 214053) but contains a protospacer.
    Depositor
    Raymond Staals
    Article
    Steens et al Science. 2024 Feb 2;383(6682):512-519. d
    Insert
    Target sequence
    Use
    Target rna plasmid
    Expression
    Bacterial
    Promoter
    trc promoter
    Available Since
    June 14, 2024
    Availability
    Academic Institutions and Nonprofits only
    214052-plasmid-map-sequence-id-428822

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  30. pH-nCas9-PPE

    Plasmid
    #140447
    Purpose
    For plant prime editing in rice plants or monocotyledons protoplasts
    Depositor
    Caixia Gao
    Article
    Lin et al Nat Biotechnol. 2020 May;38(5):582-585.
    Insert
    nCas9(H840A)-M-MLV
    Use
    CRISPR
    Expression
    Plant
    Mutation
    H840A for Cas9; D200N, T306K, W313F, T330P and L…
    Promoter
    maize Ubiquitin-1, OsU3
    Available Since
    May 5, 2020
    Availability
    Academic Institutions and Nonprofits only
    140447-plasmid-map-sequence-id-296176

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Showing: 9871 - 9900 of 10311 results