We narrowed to 17,197 results for: puromycin

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OPA1-isoform3

Plasmid

#70175

Purpose

Mammalian expression of hsOPA1 isoform 3

Depositor

David Chan

Article

Song et al J Cell Biol. 2007 Aug 27;178(5):749-55.

Insert

hsOPA1 isoform 3 (OPA1 Human)

Use

Retroviral

Expression

Mammalian

Available Since

Jan. 4, 2016

Availability

Academic Institutions and Nonprofits only
pEBTet-SNAP-CP110

Plasmid

#136802

Purpose

Mammalian expression of the centrosomal protein Cep110 N-terminally fused to SNAP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

SNAP-CP110 (CCP110 Synthetic, Human)

Tags

FLAG-tag / His-tag

Expression

Mammalian

Promoter

CMV-TetO2

Available Since

April 6, 2020

Availability

Academic Institutions and Nonprofits only

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pLKO.1-sh-mSOX9-2

Plasmid

#40645

Depositor

Bob Weinberg

Article

Guo et al Cell. 2012 Mar 2;148(5):1015-28.

Insert

sh-mSOX9-2 (Sox9 Mouse)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

hU6

Available Since

Oct. 29, 2012

Availability

Academic Institutions and Nonprofits only
OPA1-isoform4

Plasmid

#70176

Purpose

Mammalian expression of hsOPA1 isoform 4

Depositor

David Chan

Article

Song et al J Cell Biol. 2007 Aug 27;178(5):749-55.

Insert

hsOPA1 isoform 4 (OPA1 Human)

Use

Retroviral

Expression

Mammalian

Available Since

Dec. 16, 2015

Availability

Academic Institutions and Nonprofits only
PLKO-mcherry-luc-puro-shSCR

Plasmid

#29780

Depositor

Carl Novina

Article

Levy et al Mol Cell. 2010 Dec 10. 40(5):841-9.

Insert

shSCR

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Oct. 5, 2011

Availability

Academic Institutions and Nonprofits only

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pAPM-miR30-DDX58-ts3

Plasmid

#174243

Purpose

DDX58 knockdown

Depositor

Jeremy Luban

Article

Guney et al Proc Natl Acad Sci U S A. 2024 Jul 16;12

Insert

DDX58 shRNA (RIGI Human)

Use

Lentiviral and RNAi

Promoter

SFFV

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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PUC19_C_tag

Plasmid

#186659

Purpose

Empty donor plasmid for C-terminal tag of 3xFlag-3xHA.

Depositor

Astrid Haase

Article

Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

Insert

C-terminal 3xFlag-3xHA Tag

Tags

3xFlag-3xHA

Expression

Insect

Available Since

July 21, 2022

Availability

Academic Institutions and Nonprofits only

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pB-Ric8b-Integration

Plasmid

#129457

Purpose

piggyBac transposon vector that Inducibly (Tet-On) expresses Ric8b

Depositor

Sriram Kosuri

Article

Jones et al Cell Syst. 2019 Mar 27;8(3):254-260.e6.

Insert

Ric8b (Ric8b Mouse)

Use

Synthetic Biology

Expression

Mammalian

Promoter

TRE (Tet-On)

Available Since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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OPA1-isoform6

Plasmid

#70178

Purpose

Mammalian expression of hsOPA1 isoform 6

Depositor

David Chan

Article

Song et al J Cell Biol. 2007 Aug 27;178(5):749-55.

Insert

hsOPA1 isoform 6 (OPA1 Human)

Use

Retroviral

Expression

Mammalian

Available Since

Feb. 16, 2016

Availability

Academic Institutions and Nonprofits only
MSCV PIG C/EBPa

Plasmid

#66985

Purpose

Retroviral expression plasmid for mouse C/EBPa expression; containing bicistronic IRES-GFP

Depositor

Christopher Vakoc

Article

Roe et al Mol Cell. 2015 May 12. pii: S1097-2765(1

Insert

C/EBP alpha (Cebpa Mouse)

Use

Retroviral

Promoter

LTR

Available Since

July 15, 2015

Availability

Academic Institutions and Nonprofits only

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pMRX-IP/Venus-rLC3

Plasmid

#58740

Purpose

Expresses Venus-rLC3 in mammalian cells

Depositor

Noboru Mizushima

Article

Koyama-Honda et al Autophagy. 2013 Oct;9(10):1491-9. doi: 1

Insert

microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)

Use

Retroviral

Tags

Venus

Available Since

Dec. 8, 2014

Availability

Academic Institutions and Nonprofits only
RRL.sin.cPPT.CMV/Flag-E2-crimson.IRES-puro.WPRE (MT06)

Plasmid

#139448

Purpose

Lentiviral vector to ectopically express Flag-tagged E2-crimson from CMV promoter

Depositor

Caroline Goujon

Article

Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

Insert

FLAG-tag E2-crimson

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 12, 2020

Availability

Academic Institutions and Nonprofits only

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Ki67 (8 repeats+LR)-mNeonGreen-IRESpuro2

Plasmid

#183741

Purpose

Expresses Ki67 (8 repeats+LR)-mNeonGreen

Depositor

Daniel Gerlich

Article

Cuylen et al Nature. 2016 Jun 29;535(7611):308-12. do

Insert

MKI67 LR domain + 8 Ki-67 repeats (MKI67 Human)

Tags

mNeonGreen

Expression

Bacterial and Mammalian

Mutation

MKI67 LR domain + 8 Ki-67 repeats

Promoter

CMV

Available Since

July 27, 2022

Availability

Academic Institutions and Nonprofits only

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stabilized HP35 tension sensor module

Plasmid

#101251

Purpose

Expresses the stabilized HP35-based tension sensor module. The biosensor allows force measurements at 9-11 pN.

Depositor

Carsten Grashoff

Article

Austen et al Nat Cell Biol. 2015 Dec;17(12):1597-606.

Insert

YPet(short)-HP35st-mCherry

Use

Retroviral

Tags

His-tag and cysteine

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 2, 2017

Availability

Academic Institutions and Nonprofits only

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AAVS1-TetOn-3XFLAG-renilla-beta-globin-control-AAVS1

Plasmid

#184396

Purpose

Donor plasmid for stable integration of Renilla luciferase control reporter at AAVS1

Depositor

Robert Bradley

Article

Udy et al Life Sci Alliance. 2021 Dec 8;5(3). pii:

Insert

Renilla luciferase beta-globin

Use

CRISPR; Donor plasmid for hdr

Tags

3X FLAG

Expression

Mammalian

Promoter

Tet-On 3G

Available Since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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pMSCV-mCherry-Vav1

Plasmid

#50044

Purpose

Expression of mCherry-Vav1 in mammalian cells

Depositor

Hidde Ploegh

Article

Strijbis et al PLoS Pathog. 2013 Jun;9(6):e1003446. doi

Insert

Vav1 (Vav1 Mouse)

Use

Retroviral

Tags

mCherry

Expression

Mammalian

Promoter

pCMV

Available Since

Jan. 13, 2014

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(Stk11)-PGKpuroBFP-W

Plasmid

#105043

Purpose

Lentiviral gRNA plasmid targeting mouse Stk11 , co-expression of TagBFP

Depositor

Kosuke Yusa

Article

Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

Insert

Stk11 (Stk11 Mouse)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pAc-y1sgRNA-Cas9

Plasmid

#49331

Purpose

Expresses sgRNA targeting yellow gene and Cas9-Puro in Drosophila S2 cells

Depositor

Ji-Long Liu

Article

Bassett et al Biol Open. 2013 Dec 10. pii: bio.2013712

Inserts

Cas9
dU6-sgRNA
y1 sgRNA (y Fly)

Use

CRISPR

Tags

3xFLAG and NLS

Expression

Insect

Mutation

Human codon optimised

Promoter

Actin-5c and Drosophila U6

Available Since

Dec. 19, 2013

Availability

Academic Institutions and Nonprofits only

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pX62

Plasmid

#46854

Purpose

TOP Hupki-puro2A-G245S plasmid to introduce mutant p53 to PLF ESC or MEFs by IMCE

Depositor

Monica Hollstein

Article

Wei et al Cell Cycle. 2011 Apr 15;10(8):1261-70. E

Insert

attB-Puro-2A and Hupki p53 G245S (TP53 Mouse, Human)

Expression

Mammalian

Mutation

G245S

Available Since

Aug. 22, 2013

Availability

Academic Institutions and Nonprofits only

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Tet-myr-CSK K222R-GFP

Plasmid

#83471

Purpose

Lentiviral vector for doxycycline-inducible expression of membrane targeted kinase dead CSK in mammalian cells

Depositor

Barry Gumbiner

Article

Kim et al J Cell Biol. 2015 Aug 3;210(3):503-15. d

Insert

CSK (CSK Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

K222R (kinase dead)

Promoter

doxycycline-inducible promoter

Available Since

Oct. 7, 2016

Availability

Academic Institutions and Nonprofits only

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