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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 7,984 results for: ski

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Showing: 6581 - 6600 of 7984 results

  4. pAAVS1-NDi-CRISPRi (Gen1)

    Plasmid
    #73497
    Purpose
    Dox-inducible CRISPR interference (CRISPRi) knock in construct into the AAVS1 locus with mCherry marker
    Depositor
    Bruce Conklin
    Article
    Mandegar et al Cell Stem Cell. 2016 Apr 7;18(4):541-53.
    Inserts
    dCas9-KRAB-P2A-mCherry
    rtTA
    Use
    CRISPR
    Tags
    HA, KRAB, and NLS
    Expression
    Mammalian
    Mutation
    D10A, H840A (catalytically deactivated Cas9)
    Promoter
    CAG and TRE3G
    Available Since
    Dec. 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    73497-plasmid-map-sequence-id-247304

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  10. pLV-UbC-Tet1v4-dCas9

    Plasmid
    #232180
    Purpose
    Tet1v4-dCas9 from Nuñez et al. 2021, in a lentiviral cassette with blasticidin resistance, for expression in mammalian cells
    Depositor
    Charles Gersbach
    Article
    Rohm et al Cell Genom. 2025 Feb 12;5(2):100770. doi
    Insert
    Tet1 catalytic domain - XTEN80 linker - dead Cas9 - 2A - BlastR (TET1 S. pyogenes, Human)
    Use
    Lentiviral
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    D10A, H840A
    Promoter
    hUbC
    Available Since
    March 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    232180-plasmid-map-sequence-id-462961

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  12. RG6-BAP1_E11-G312G

    Plasmid
    #154026
    Purpose
    Bichromatic Fluorescent Splicing Reporter Minigene for Mutant BAP1 Exon 11 c.936T>G, p.G312G (Synonymous Mutation)
    Depositor
    Samuel Peña-Llopis
    Article
    Niersch et al iScience. 2021 Feb 12;24(3):102173. doi:
    Insert
    BAP1 Exon 11 Fused to DsRed1 and eGFP (BAP1 Human)
    Use
    Splicing reporter
    Tags
    DsRed1, FLAG, SV40 NLS, and eGFP
    Expression
    Mammalian
    Mutation
    BAP1 c.936T>G, p.G312G
    Available Since
    Feb. 12, 2021
    Availability
    Academic Institutions and Nonprofits only
    154026-plasmid-map-sequence-id-307080

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  17. pOT_5 - lenti-EFS-FMC6.3-28z-2A-puro-2A-tNGFR

    Plasmid
    #181974
    Purpose
    Expresses anti-CD19 CAR (with CD28 signaling domain) linked to puromycin resistance via P2A and to human truncated NGFR via T2A for lentiviral delivery.
    Depositor
    Neville Sanjana
    Article
    Legut et al Nature. 2022 Mar;603(7902):728-735. doi:
    Insert
    FMC6.3-28z CAR; tNGFR (NGFR Human, Synthetic)
    Use
    Lentiviral
    Mutation
    NGFR truncation: aa 11-276
    Available Since
    April 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    181974-plasmid-map-sequence-id-355578

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Showing: 6581 - 6600 of 7984 results