We narrowed to 14,777 results for: BASE
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Plasmid#102466PurposeExpresses sigma 54 protein in E. coli. The expression is controlled by a OR2-OR1-Pr promoter.DepositorArticleAvailable SinceOct. 25, 2017AvailabilityAcademic Institutions and Nonprofits only
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pJEP303-pAAV-CMV-dSaCas9-VP64-pA
Plasmid#113678PurposeA CMV driven de-catalyzed SaCas9 fused to VP64 domain for increased transcription in targeted regionDepositorInsertde-catalyzed SaCas9
UseAAV, CRISPR, and Synthetic BiologyTagsNLS and VP64Available SinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
Lenti_Efs_hSpCas9_NLS_FLAG-WPRE
Plasmid#97313PurposeLentiviral vector for encoding a human codon-optimized SpCas9 driven by EFs promoter.DepositorInserthumanized S. pyogenes Cas9
UseLentiviral and Mouse TargetingTagsFlagExpressionMammalianPromoterEFSAvailable SinceAug. 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGP-CAG-Ace2N-4AA-mNeon A122D-WPRE-bGH-polyA
Plasmid#172911PurposeMammalian expression of voltage sensorDepositorInsertAce2N-4AA-mNeon A122D
Tagssoma localization targeting sequenceExpressionMammalianMutationA122DPromoterCAGAvailable SinceNov. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
alpha 0-CFP in pdest12.2
Plasmid#36312DepositorInsertG protein alpha 0 (Gnao1 Rat)
TagsCFPExpressionMammalianMutationIntroduced KpnI and SpeI sites between 92nd and 9…PromoterCMVAvailable SinceFeb. 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET28a(+)-SpdCas9-LBiT_Strep-Tag-II
Plasmid#198751PurposeExpresses S. Pyogenes dCas9 fused to LargeBiT fragment of split NanoLuc luciferaseDepositorInsertSpdCas9 - LargeBiT
TagsStrep-tag IIExpressionBacterialMutationD10A, H840A (nuclease inactivating mutations in &…PromoterT7Available SinceJune 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
Tol2-LyzC-Cas9-pA-Cry-GFP
Plasmid#168239Purpose"neutrophil specific cas9 expression in zebrafish with green lens"DepositorInsertCas9
UseZebrafish expressionAvailable SinceMarch 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRGEN-CMV-VPR-L1-CjCas9 V-D
Plasmid#169913PurposeExpression of a VPR-dCjCas9 fusion protein to activate gene expression.DepositorInsertdCjCas9
UseCRISPRTags2xSV40NLS, SV40NLS-HA, and VPR (VP64-p65-Rta)ExpressionMammalianMutationD8A, H559A in cjCas9PromoterCMVAvailable SinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRL692
Plasmid#70284PurposeTn5-based plasmid for transposition in Synechococcus sp. strain PCC 7942DepositorInsertTransposon Tn5-692
ExpressionBacterialAvailable SinceDec. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pScaf-3024.1
Plasmid#111404PurposepScaf phagemid with insert for generating DNA origami scaffold 3024 bases in lengthDepositorInsertpScaf-3024
ExpressionBacterialAvailable SinceAug. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMi(3xP3-DsRed)
Plasmid#102539PurposeTransgenesis vector based on the Minos transposon, carrying the 3xP3-DsRed markerDepositorInsertMinos transpoable element with 3xP3-DsRed marker
Available SinceMarch 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
8xGliBS-IVS2-mCherry-NLS-polyA-Tol2
Plasmid#84604PurposeTol2-based hedgehog signal reporter plasmid for zebrafish transgenesisDepositorInsertmCherry
UseZebrafish transgenesisPromoter8xGliBS-delta-crystallin minimal promoterAvailable SinceOct. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV_GR-RE_MLPmin_BC1092-luc2
Plasmid#227112PurposeGlucocorticoid receptor response element for luciferase and barcode assays; barcode BC1092DepositorInsert12x clustered GR response element linked to MLP minimal promoter driving barcode 1092 and a luciferase reporter gene
UseAAVExpressionMammalianAvailable SinceDec. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
qTAG-C-Solo-mStayGold-COX8A
Plasmid#227309PurposeDonor template for mStayGold insertion into the C-terminus of the COX8A locus. For mitochondria visualization. To be co-transfected with sgRNAplasmid px330-PITCh-COX8A (Addgene #227308)DepositorInsertCOX8A Homology Arms flanking a mStayGold Tag (COX8A Human)
UseCRISPR; Donor templateExpressionMammalianPromoterPromoterlessAvailable SinceNov. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
qTAG-C-mStayGold-Puro-H2BC11
Plasmid#227332PurposeDonor template for mStayGold-2A-Puro insertion into the C-terminus of the H2BC11 locus. For nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 (Addgene #207755)DepositorInsertH2BC11 Homology Arms flanking a mStayGold-2A-Puro Cassette (H2BC11 Human)
UseCRISPR; Donor templateExpressionMammalianPromoterPromoterlessAvailable SinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
qTAG-N-Solo-mStayGold-TUBA1B
Plasmid#227327PurposeDonor template for mStayGold insertion into the N-terminus of the TUBA1B locus. For tubulin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TUBA1B (Addgene #207763)DepositorInsertTUBA1B Homology Arms flanking a mStayGold Tag (TUBA1B Human)
UseCRISPR; Donor templateExpressionMammalianPromoterPromoterlessAvailable SinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
qTAG-N-Solo-mStayGold-ACTB
Plasmid#227326PurposeDonor template for mStayGold insertion into the N-terminus of the ACTB locus. For actin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-ACTB (Addgene #207748)DepositorInsertACTB Homology Arms flanking a mStayGold Tag (ACTB Human)
UseCRISPR; Donor templateExpressionMammalianPromoterPromoterlessAvailable SinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
qTAG-C-Solo-mStayGold-LAMP1
Plasmid#227322PurposeDonor template for mStayGold insertion into the C-terminus of the LAMP1 locus. For lysosome visualization. To be co-transfected with sgRNA plasmid px330-LAMP1 (Addgene #207787)DepositorInsertLAMP1 Homology Arms flanking a mStayGold Tag (LAMP1 Human)
UseCRISPR; Donor templateExpressionMammalianPromoterPromoterlessAvailable SinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
qTAG-C-mStayGold-Puro-PXN
Plasmid#227317PurposeDonor template for mStayGold-2A-Puro insertion into the C-terminus of the PXN locus. For focal adhesion visualization. To be co-transfected with sgRNA plasmid px330-PITCh-PXN (Addgene #227315)DepositorInsertPXN Homology Arms flanking a mStayGold-2A-Puro Cassette (PXN Human)
UseCRISPR; Donor templateExpressionMammalianPromoterPromoterlessAvailable SinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
qTAG-N-Solo-mStayGold-CETN2
Plasmid#227292PurposeDonor template for mStayGold insertion into the N-terminus of the CETN2 locus. For centriole visualization. To be co-transfected with sgRNA plasmid px330-CETN2 (Addgene #227291)DepositorInsertCETN2 Homology Arms flanking a mStayGold Tag (CETN2 Human)
UseCRISPR; Donor templateExpressionMammalianPromoterPromoterlessAvailable SinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only