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mycUBB+1

Plasmid

#20254

Depositor

Nico Dantuma

Article

Verhoef et al FASEB J. 2009 Jan . 23(1):123-33.

Insert

Ubiquitin B +1 transcript (UBB Human)

Tags

myc

Expression

Mammalian

Available Since

April 14, 2009

Availability

Academic Institutions and Nonprofits only

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dSp-VP64-p65-RTA(75-190)

Plasmid

#99670

Purpose

Expresses dSp Cas9 fused to combination of truncated activation domains

Depositor

George Church

Article

Vora et al bioRxiv 2018. 298620

Insert

dCas9

Tags

VP64-p65-RTA(75-190)

Expression

Mammalian

Mutation

dead Cas9

Available Since

May 30, 2020

Availability

Academic Institutions and Nonprofits only

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pEC2735 (pSpy0C2)

Plasmid

#220279

Purpose

integrative plasmid for heterologous gene expression in S. pyogenes SF370, integrates into the amyA locus via homologous recombination

Depositor

Emmanuelle Charpentier

Article

Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

Type

Empty backbone

Expression

Bacterial

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pMC-1-SlUbi-6

Plasmid

#197709

Purpose

Phytobrick (MoClo) Level 0 Part

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Insert

SlUbi promoter

Expression

Plant

Available Since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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pMC-9-SlUbi-11

Plasmid

#197773

Purpose

Phytobrick (MoClo) Level 0 Part

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Insert

SlUbi terminator

Expression

Plant

Available Since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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pIM1708-surT-PsurP-agaN-pNW33N

Plasmid

#44009

Depositor

Ichiro Matsumura

Article

Blanchard et al Appl Microbiol Biotechnol. 2014 May 2.

Insert

surT-PsurP-agaN

Expression

Bacterial

Mutation

Deleted EcoRI site (synonymous change, C to T at …

Available Since

May 15, 2014

Availability

Academic Institutions and Nonprofits only

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pJZC603

Plasmid

#62317

Purpose

sgRNA with 2x PP7 for yeast cells

Depositor

Wendell Lim, Stanley Qi

Article

Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

Insert

sgRNA + 2x PP7 RNA binding module

Expression

Yeast

Promoter

SNR52

Available Since

March 18, 2015

Availability

Academic Institutions and Nonprofits only

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PET28a-cpLumiLuc-Rapamycin sensor

Plasmid

#186292

Purpose

Expression Rapamycin sensor in e coli

Depositor

Kirill Alexandrov

Article

Guo et al Nat Commun. 2022 Feb 10;13(1):789. doi:

Insert

cpLumiLuc-Rapamycin sensor

Tags

HisTag

Expression

Bacterial

Available Since

Aug. 26, 2022

Availability

Academic Institutions and Nonprofits only

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T. aq RNA Polymerase

Plasmid

#215349

Purpose

Express T. aq RNA Polymerase subunits in E. coli cells

Depositor

Julius Lucks

Article

Hertz et al Nucleic Acids Res. 2024 May 8;52(8):4466

Insert

RNA polymerase

Tags

6x His

Expression

Bacterial

Available Since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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pRS425ADS

Plasmid

#20119

Depositor

Jay Keasling

Article

Ro et al Nature. 2006 Apr 13. 440(7086):940-3.

Insert

amorphadiene synthase

Use

Synthetic Biology

Expression

Yeast

Available Since

March 2, 2009

Availability

Academic Institutions and Nonprofits only
pLH-sgRNA1-MS2-PP7

Plasmid

#75392

Purpose

sgRNA1-MS2-PP7

Depositor

Thoru Pederson

Article

Ma et al Nat Biotechnol. 2016 Apr 18. doi: 10.103

Insert

sgRNA1-2XMS2-PP7

Use

CRISPR and Lentiviral

Tags

no

Expression

Mammalian

Promoter

U6

Available Since

May 25, 2016

Availability

Academic Institutions and Nonprofits only

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cpLOV2-V5

Plasmid

#168991

Purpose

circularly permuted LOV2 variant-V5

Depositor

Yubin Zhou

Article

He et al Nat Chem Biol. 2021 Aug;17(8):915-923. d

Insert

cpLOV2-V5

Expression

Mammalian

Promoter

CMV

Available Since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

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pLH-sgRNA1-PP7-boxB

Plasmid

#75393

Purpose

sgRNA1-PP7-boxB

Depositor

Thoru Pederson

Article

Ma et al Nat Biotechnol. 2016 Apr 18. doi: 10.103

Insert

sgRNA1-2XPP7-boxB

Use

CRISPR and Lentiviral

Tags

no

Expression

Mammalian

Promoter

U6

Available Since

May 25, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-ef1a-DIO-eGCaMP(2+)

Plasmid

#201150

Purpose

AAV virus production for Cre recombinase dependent expression of eGCaMP(2+)

Depositor

Andre Berndt

Article

Wait et al Res Sq. 2023 Aug 7:rs.3.rs-3146778. doi:

Insert

eGCaMP_2+

Use

AAV, Cre/Lox, and Mouse Targeting

Expression

Mammalian

Available Since

June 13, 2023

Availability

Academic Institutions and Nonprofits only

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pLP3

Plasmid

#209958

Purpose

Entry vector to store Level 0 broad-host range L. plantarum replication origin parts

Depositor

Thorsten Mascher

Article

Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

Type

Empty backbone

Expression

Bacterial

Available Since

Sept. 13, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pMD18-T-pyrF

Plasmid

#174388

Purpose

modified pMD18-T containing pyrF and its native promoter, Ampr

Depositor

Jing Han

Article

Lin et al Commun Biol. 2021 Aug 25;4(1):1007. doi:

Insert

pyrF

Use

Template for cloning fragments for chromosomal in…

Expression

Bacterial

Available Since

Nov. 11, 2021

Availability

Academic Institutions and Nonprofits only

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Golgi-targeted optodPLD

Plasmid

#140057

Purpose

Encodes CRY2-mCherry-dPLD-P2A-CIBN-Sialyltransferase(TM) for mammalian expression

Depositor

Jeremy Baskin

Article

Tei et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

Phospholipase D

Tags

CIBN, CRY2, P2A, Sialyltransferase(TM), and mCher…

Expression

Mammalian

Mutation

H170A (catalytic dead)

Available Since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-luxGold

Plasmid

#229948

Purpose

Mammalian expression of a de novo designed, FRET-based neoLux1.2-mGold fusion bioluminescent probe (max. Em=530nm)

Depositor

Andy Yeh

Article

Chen et al Chem. 2025 Mar 13;11(3):102346. doi: 10.

Insert

luxGold

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pCellFree_G03 WWP1

Plasmid

#67125

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

WWP1 (WWP1 Human)

Use

Cell-free expression

Tags

EGFP and His

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only

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pKSB-609 U6-sgRNA1 ATCC GGAA

Plasmid

#173671

Purpose

To clone and express a gRNA in Anopheles

Depositor

Eric Marois

Article

Klug et al PLoS Pathog. 2022 Oct 12;18(10):e1010881

Type

Empty backbone

Use

CRISPR

Expression

Insect

Available Since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-K20

Plasmid

#14743

Depositor

M Bishr Omary

Article

Zhou et al Mol Biol Cell. 2003 Jul . 14(7):2959-71.

Insert

Keratin 20 (KRT20 Human)

Expression

Mammalian

Available Since

April 6, 2007

Availability

Academic Institutions and Nonprofits only
pCMrfp

Plasmid

#59773

Purpose

Derived from pCM62; ampR, tetR, PBAD - rfp

Depositor

Nathan Hillson

Article

Bi et al Microb Cell Fact. 2013 Nov 13;12:107. do

Insert

rfp

Expression

Bacterial

Promoter

pBAD

Available Since

Oct. 24, 2014

Availability

Academic Institutions and Nonprofits only

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pCAG APX W41F dimeric (mito matrix)

Plasmid

#40307

Depositor

Alice Ting

Article

Martell et al Nat Biotechnol. 2012 Oct 21. doi: 10.103

Insert

pea APX W41F (dimeric)

Tags

Flag

Mutation

W41F mutation.

Promoter

CAG

Available Since

Oct. 25, 2012

Availability

Academic Institutions and Nonprofits only

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pI6-MTU-DO-URA

Plasmid

#160030

Purpose

Integrative vector for K. marxianus, targeting the ARO3 locus. Uracil selection. Low integration efficiency in an NHEJ-deficient background.

Depositor

John Morrissey

Article

Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

Type

Empty backbone

Use

Kluyveromyces marxianus

Expression

Yeast

Promoter

n/a

Available Since

Dec. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pQadd2

Plasmid

#135662

Purpose

Clostridial vector, carrying lox511/66 and loxFAS/71 sites separated by restriction sites in order to add cargo. Use in combination with pQcre1 to insert cargo into lox sites, inserted with pQadd1F/R.

Depositor

Andrew Tolonen

Article

Cerisy et al mSphere. 2019 Dec 11;4(6). pii: 4/6/e007

Insert

Lox511/66 and LoxFas/71

Use

Clostridial vector, pamβ1 origin

Expression

Bacterial

Available Since

July 15, 2020

Availability

Academic Institutions and Nonprofits only

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pCAG-T3-eSpCAS9-NG-pA

Plasmid

#131466

Purpose

Expresses eSpCAS9-NG nuclease in mammalian cells. Used to modify genome of mouse embroys

Depositor

Wataru Fujii

Article

Fujii et al Sci Rep. 2019 Sep 9;9(1):12878. doi: 10.

Insert

Codon optimized eSpCas9-NG

Use

CRISPR

Tags

Flag, NLS, and polyA

Expression

Mammalian

Available Since

Nov. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pDEINO5

Plasmid

#179489

Purpose

Multi-host shuttle plasmid for E. coli, D. radiodurans, and S. cerevisiae; contains an oriT for transfer via conjugation

Depositor

Bogumil Karas

Article

Brumwell et al ACS Synth Biol. 2022 Mar 18;11(3):1068-1

Insert

aacC1

Use

Synthetic Biology; Bacterial and yeast cloning

Available Since

May 10, 2022

Availability

Academic Institutions and Nonprofits only

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pDM1209

Plasmid

#108986

Purpose

extrachromosomal expression vector, C-terminal GFP tagging

Depositor

Rob Kay

Article

Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

Type

Empty backbone

Use

Dictyostelium discoideum expression vector

Tags

GFP

Available Since

July 19, 2018

Availability

Academic Institutions and Nonprofits only

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pDM1079

Plasmid

#108979

Purpose

Knockout cassette Blasticidin

Depositor

Rob Kay

Article

Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

Insert

Knockout cassette Blasticidin

Use

Cre/Lox; Dictyostelium discoideum knockout vector

Promoter

coaA

Available Since

July 18, 2018

Availability

Academic Institutions and Nonprofits only

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CAG-EGFP-LacZshRNA

Plasmid

#73977

Purpose

LacZshRNA (mir155 backbone) was inserted into the 3'UTR of EGFP.

Depositor

Connie Cepko

Article

Wang et al Dev Cell. 2014 Sep 8;30(5):513-27. doi:

Insert

shRNA that targets the LacZ gene

Expression

Mammalian

Promoter

CAG

Available Since

May 4, 2016

Availability

Academic Institutions and Nonprofits only

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