We narrowed to 12,282 results for: cel.2

1. tetO-NR2F1

   Plasmid

   #170698

   Purpose

   doxycycline-inducible overexpression of NR2F1

   Depositor

   Paul Burridge

   Article

   Romero-Tejeda et al J Mol Cell Cardiol. 2023 Jul 6:S0022-282

   Insert

   nuclear receptor subfamily 2 group F member 1 (NR2F1 Human)
   

   Use

   Lentiviral; Doxycycline inducible

   Expression

   Mammalian

   Mutation

   deletion of 36G- please see depositor comments

   Promoter

   TRE promoter, Tet-ON

   Available Since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pSems-leader-mEGFP-MBP-TMD(ALA7)

   Plasmid

   #193051

   Purpose

   Expression of artificial transmembrane protein tagged with mEGFP and MBP at the plasma membrane. Control construct for single molecule co-localization and co-tracking microscopy.

   Depositor

   Jacob Piehler

   Article

   Sotolongo Bellon et al Cell Rep Methods. 2022 Feb 4;2(2):100165

   Insert

   ALA7-TMD
   

   Tags

   Ig k-chain leader sequence, MBP, and mEGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pRSFDuet-1_VNp-LZ_VNp-LZ-mNeongreen-hGH

   Plasmid

   #182425

   Purpose

   Bacterial expression of Vesicle Nucleating peptide-Leucine Zipper and Vesicle Nucleating peptide-Leucine Zipper-mNeongreen-human growth hormone fusion proteins

   Depositor

   Dan Mulvihill

   Article

   Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

   Inserts

   VNp-LZ-mNeongreen-hGH (GH1 Human)
   VNp-LZ
   

   Tags

   Vesicle Nucleating peptide (VNp) and Vesicle Nucl…

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pSH-Csy4-T2A-SpRFN

   Plasmid

   #85754

   Purpose

   Expresses S. pyogenes FokI-dCas9-NLS with a 25 amino acid linker (GGGGS)5 fusion. Also expresses Csy4 for cleavage of multiplexed gRNA transcripts. Derived from pSQT1601 (Addgene #53369).

   Depositor

   Lawrence Stanton

   Article

   Havlicek et al Mol Ther. 2017 Feb 1;25(2):342-355. doi:

   Insert

   Csy4-T2A-FokI-dCas9
   

   Use

   CRISPR

   Tags

   Csy4 and FokI fusion via 25 amino acid (GGGGS)5 l…

   Expression

   Mammalian

   Mutation

   D10A, H840A

   Promoter

   CAG

   Available Since

   Jan. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pMK297 (DHC1-mAID Hygro)

   Plasmid

   #140542

   Purpose

   DHC1 tagging with mAID

   Depositor

   Masato Kanemaki

   Article

   Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

   Insert

   DHC1 (DYNC1H1 Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Nov. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pSH-Csy4-T2A-SaRFN

   Plasmid

   #85755

   Purpose

   Expresses S.aureus FokI-dCas9-NLS with a 36 amino acid GSAT linker fusion. Also expresses Csy4 for cleavage of multiplexed gRNA transcripts. Backbone derived from pSQT1601 (Addgene #53369).

   Depositor

   Lawrence Stanton

   Article

   Havlicek et al Mol Ther. 2017 Feb 1;25(2):342-355. doi:

   Insert

   Csy4-T2A-FokI-SadCas9
   

   Use

   CRISPR

   Tags

   3xHA tag, Csy4, and FokI fusion via 36 amino acid…

   Expression

   Mammalian

   Mutation

   D10A, N580A

   Promoter

   CAG

   Available Since

   Jan. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pMK296 (DHC1-mAID Neo)

   Plasmid

   #140541

   Purpose

   DHC1 tagging with mAID

   Depositor

   Masato Kanemaki

   Article

   Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

   Insert

   DHC1 (DYNC1H1 Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Nov. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. DHC1-mIAA7 Neo

   Plasmid

   #140543

   Purpose

   DHC1 tagging with mIAA7

   Depositor

   Masato Kanemaki

   Article

   Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

   Insert

   DHC1 (DYNC1H1 Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Nov. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCDNA5-LAP-MPS1-d200

   Plasmid

   #114047

   Purpose

   Expression of exogenous LAP tagged MPS1 delta 200 (NTE and TPR are deleted)

   Depositor

   Geert Kops

   Article

   Nijenhuis et al J Cell Biol. 2013 Apr 15;201(2):217-31.

   Insert

   MPS1-d200 (TTK Human)
   

   Expression

   Mammalian

   Mutation

   delta 200 (deletion of NTE and TPR domains)

   Available Since

   Sept. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA3.1_hHNF4alpha

    Plasmid

    #206878

    Purpose

    The ORF of HNF4α (variant 2) cDNA amplified from the RT human liver RNA was cloned at the Bam HI/XbaI sites of the pcDNA3.1(+), the mammalian expression vector.

    Depositor

    Catharine Ross, Reza Zolfaghari

    Article

    Zolfaghari et al J Cell Biochem. 2014 Oct;115(10):1740-51

    Insert

    Human hepatocyte nuclear factor 4 alpha (HNF4A Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pMXS-IRES-BLAST flag-CAD T456A S1406A S1859A

    Plasmid

    #169826

    Purpose

    Expresses C-terminal flag-tagged CAD with mutation of reported S6K, PKA, and Erk1/2 phosphorylation site

    Depositor

    Richard Possemato

    Article

    Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

    Insert

    CAD (CAD Human)
    

    Use

    Retroviral

    Tags

    Flag tag

    Expression

    Mammalian

    Mutation

    T456A S1406A S1859A; TCCC -> AGTC silent mutat…

    Available Since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. CRY2low-tdTomato-Raf1

    Plasmid

    #104176

    Purpose

    Expresses fusion of CRY2low mutant (CRY2 aa1-491 R489E A491D) with tdTomato and Raf1

    Depositor

    Bianxiao Cui

    Article

    Duan et al Nat Commun. 2017 Sep 15;8(1):547. doi: 1

    Insert

    CRY2low-tdTomato-Raf1 (CRY2, RAF1 Mustard Weed, Human)
    

    Expression

    Mammalian

    Mutation

    CRY2 aa 1-491 truncation, R489E A491D

    Promoter

    CMV

    Available Since

    Jan. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. CRY2low-mCherry-Raf1

    Plasmid

    #104066

    Purpose

    Expresses fusion of CRY2low mutant (CRY2 aa1-491 R489E A491D) with mCherry and Raf1

    Depositor

    Bianxiao Cui

    Article

    Duan et al Nat Commun. 2017 Sep 15;8(1):547. doi: 1

    Insert

    CRY2low-mCherry-Raf1 (CRY2, RAF1 Mustard Weed, Human)
    

    Expression

    Mammalian

    Mutation

    CRY2 aa 1-491 truncation, R489E A491D

    Promoter

    CMV

    Available Since

    Dec. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pTK41

    Plasmid

    #236110

    Purpose

    Expresses the motor domain of rat KIF5C fused with SNAP tag and ALFA tag nanobody

    Depositor

    Shinsuke Niwa

    Article

    Kita et al Cell Rep Phys Sci, 6 (2): 102440. 19 Feb

    Inserts

    KIF5C(C7S)(1-430)-SNAP-His (Kif5c Rat)
    KIF5C(C7S)(1-430)-ALFA tag nanobody-StrepII tag (Kif5c Rat)
    

    Tags

    ALFA tag nanobody, His tag, SNAP tag, and StrepII…

    Expression

    Bacterial

    Mutation

    C7S mutation is introduced. 1-430 aa is encoded.

    Promoter

    T7

    Available Since

    May 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pMXS-IRES-BLAST flag-CAD T456A S1406A

    Plasmid

    #169823

    Purpose

    Expresses C-terminal flag-tagged CAD with mutation of reported Erk1/2 and PKA phosphorylation site

    Depositor

    Richard Possemato

    Article

    Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

    Insert

    CAD (CAD Human)
    

    Use

    Retroviral

    Tags

    Flag tag

    Expression

    Mammalian

    Mutation

    T456A S1406A; TCCC -> AGTC silent mutations at…

    Available Since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pMXS-IRES-BLAST flag-CAD T456A S1859A

    Plasmid

    #169824

    Purpose

    Expresses C-terminal flag-tagged CAD with mutation of reported Erk1/2 and S6K phosphorylation site

    Depositor

    Richard Possemato

    Article

    Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

    Insert

    CAD (CAD Human)
    

    Use

    Retroviral

    Tags

    Flag tag

    Expression

    Mammalian

    Mutation

    T456A S1859A; TCCC -> AGTC silent mutations at…

    Available Since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pTT3-ecdCD86-ST3-His

    Plasmid

    #210664

    Purpose

    Expression of the extracellular domain of human CD86 fused to Spytag003 + Histag in HEK293 (or similar)

    Depositor

    Omer Dushek

    Article

    Patel et al EMBO J. 2024 Jan;43(1):132-150. doi: 10.

    Insert

    Extracellular domain of human CD86 fused to Spytag003 and Histag (CD86 Human)
    

    Tags

    Histag and Spytag003

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pD649-HAsp-IL1R2-COMP5AP-AviTag-9xHis

    Plasmid

    #157333

    Purpose

    Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    IL1R2 (IL1R2 Human)
    

    Tags

    COMP5AP-AviTag-9xHis

    Expression

    Mammalian

    Promoter

    CMV/SP6

    Available Since

    Feb. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. ACPPiso2-V5

    Plasmid

    #113053

    Purpose

    Expresses human prostatic acid phosphatase (isoform 2) in mammalian cells

    Depositor

    Federico Sesti

    Article

    Cotella et al FASEB J. 2013 Apr;27(4):1381-93. doi: 10

    Insert

    prostatic acid phosphatase isoform 2 (ACP3 Human)
    

    Tags

    V5

    Expression

    Mammalian

    Available Since

    Aug. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAAV-hSyn-FRISZ-TM

    Plasmid

    #176886

    Purpose

    AAV transfer plasmid for cell-surface-localized FRISZ (via PDGFR TM) under hSyn promoter

    Depositor

    Huiwang Ai

    Article

    Wu et al Sci Adv. 2023 Mar;9(9):eadd2058. doi: 10

    Insert

    Ig kappa-FRISZ-PDGFR TM
    

    Use

    AAV

    Promoter

    hSyn

    Available Since

    Feb. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge