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pKS_3MYC_PAC

Plasmid

#122566

Purpose

Vector for endogenously tagging Giardia genes with a triple Myc tag (Puromycin selection)

Depositor

Zacheus Cande, Alexander Paredez

Article

Gourguechon et al Eukaryot Cell. 2011 Jan;10(1):142-5. doi

Type

Empty backbone

Tags

3x c-Myc tag

Available Since

April 27, 2023

Availability

Academic Institutions and Nonprofits only

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pKS_3HA_NEO

Plasmid

#122565

Purpose

Vector for endogenously tagging Giardia genes with a triple HA tag (G418 selection)

Depositor

Zacheus Cande, Alexander Paredez

Article

Gourguechon et al Eukaryot Cell. 2011 Jan;10(1):142-5. doi

Type

Empty backbone

Tags

hemagglutinin tag

Available Since

April 27, 2023

Availability

Academic Institutions and Nonprofits only

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pUG-233

Plasmid

#104710

Purpose

Cloning Module

Depositor

Marco Trujillo

Article

Kowarschik et al New Phytol. 2018 Mar;217(4):1749-1763. d

Insert

AvrPtoB WT

Use

Synthetic Biology

Available Since

Feb. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pUG-234

Plasmid

#104711

Purpose

Cloning Module

Depositor

Marco Trujillo

Article

Kowarschik et al New Phytol. 2018 Mar;217(4):1749-1763. d

Insert

AvrPtoB F479A

Use

Synthetic Biology

Mutation

F479A

Available Since

Feb. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pUG-TP6

Plasmid

#104654

Purpose

Level 1 cloning vector

Depositor

Marco Trujillo

Article

Kowarschik et al New Phytol. 2018 Mar;217(4):1749-1763. d

Type

Empty backbone

Use

Synthetic Biology

Available Since

Feb. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pUG-TP2

Plasmid

#104650

Purpose

Level 1 cloning vector

Depositor

Marco Trujillo

Article

Kowarschik et al New Phytol. 2018 Mar;217(4):1749-1763. d

Type

Empty backbone

Use

Synthetic Biology

Available Since

Feb. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pUG-P1

Plasmid

#104643

Purpose

Level 1 cloning vector

Depositor

Marco Trujillo

Article

Kowarschik et al New Phytol. 2018 Mar;217(4):1749-1763. d

Type

Empty backbone

Use

Synthetic Biology

Available Since

Feb. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pZE27GFP3A

Plasmid

#75459

Purpose

Plasmid for constitutive expression of gfp-pdt3A

Depositor

James Collins

Article

Cameron et al Nat Biotechnol. 2014 Dec;32(12):1276-81.

Insert

gfp-pdt#3A

Expression

Bacterial

Promoter

placIq promoter

Available Since

Oct. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pZE27GFP3D

Plasmid

#75462

Purpose

Plasmid for constitutive expression of gfp-pdt3D

Depositor

James Collins

Article

Cameron et al Nat Biotechnol. 2014 Dec;32(12):1276-81.

Insert

gfp-pdt#3D

Expression

Bacterial

Promoter

placIq promoter

Available Since

Oct. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pZE27GFP0

Plasmid

#75453

Purpose

Plasmid for constitutive expression of gfp-pdt

Depositor

James Collins

Article

Cameron et al Nat Biotechnol. 2014 Dec;32(12):1276-81.

Insert

gfp-pdt

Expression

Bacterial

Promoter

placIq promoter

Available Since

Oct. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pZE27GFP2

Plasmid

#75455

Purpose

Plasmid for constitutive expression of gfp-pdt2

Depositor

James Collins

Article

Cameron et al Nat Biotechnol. 2014 Dec;32(12):1276-81.

Insert

gfp-pdt#2

Expression

Bacterial

Promoter

placIq promoter

Available Since

Oct. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pZE27GFP4

Plasmid

#75457

Purpose

Plasmid for constitutive expression of gfp-pdt4

Depositor

James Collins

Article

Cameron et al Nat Biotechnol. 2014 Dec;32(12):1276-81.

Insert

gfp-pdt#4

Expression

Bacterial

Promoter

placIq promoter

Available Since

Oct. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pSUPER-DsRed

Plasmid

#42053

Depositor

Kumiko Ui-Tei

Article

Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

Insert

DsRed shRNA

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

March 1, 2013

Availability

Academic Institutions and Nonprofits only

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pET-GST

Plasmid

#42049

Depositor

Kumiko Ui-Tei

Article

Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

Insert

GST

Tags

His

Expression

Bacterial

Available Since

March 6, 2013

Availability

Academic Institutions and Nonprofits only

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Actin-cpstFRET-Actin

Plasmid

#80643

Purpose

cpstFRET actin sensor for mechanical force measurements in cytoskeleton

Depositor

Fred Sachs

Article

Guo et al Proc Natl Acad Sci U S A. 2014 Dec 9;111

Insert

Beta actin, cpstFRET

Expression

Mammalian

Available Since

Aug. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pET-6xHis-sfGFP

Plasmid

#199171

Purpose

used to express a superfolder GFP in E. coli for comparison to supercharged variants

Depositor

Allie Obermeyer

Article

Yeong et al ACS Cent Sci. 2020 Dec 23;6(12):2301-231

Insert

superfolder GFP

Tags

MGHHHHHHGGA

Promoter

T7

Available Since

May 10, 2023

Availability

Academic Institutions and Nonprofits only

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pXP842

Plasmid

#46068

Depositor

Nancy DaSilva

Article

Shen et al Yeast. 2012 Dec;29(12):495-503. doi: 10.

Type

Empty backbone

Expression

Yeast

Promoter

ADH2

Available Since

Sept. 10, 2013

Availability

Academic Institutions and Nonprofits only

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pPTP1BD181A-mCherry

Plasmid

#40270

Depositor

Martin Offterdinger

Article

Offterdinger et al Traffic. 2008 Jan;9(1):147-55. Epub 2007

Insert

PTPN1 (PTPN1 Human)

Tags

mCherry

Expression

Mammalian

Mutation

D181A

Promoter

CMV

Available Since

Nov. 26, 2012

Availability

Academic Institutions and Nonprofits only

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pHimar6

Plasmid

#137081

Purpose

bacterial Himar1 mini-transposon donor plasmid

Depositor

Harris Wang

Article

Chen et al CRISPR J. 2019 Dec;2(6):376-394. doi: 10

Insert

Himar1 mini-transposon with chloramphenicol resistance gene

Use

Synthetic Biology

Expression

Bacterial

Available Since

March 5, 2020

Availability

Academic Institutions and Nonprofits only

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pBADZ-HisCre

Plasmid

#111187

Purpose

Helper plasmid containing Cre-recombinase gene, which is expressed in DH10MultiBac cells

Depositor

Tim Richmond

Article

Berger et al Nat Biotechnol. 2004 Dec;22(12):1583-7.

Insert

Cre recombinase

Tags

His tag

Expression

Bacterial

Available Since

June 21, 2018

Availability

Academic Institutions and Nonprofits only

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pECGMC3

Plasmid

#75441

Purpose

Plasmid for mf-lon mediated mCherry degradation in Lactococcus lactis. Contains mcherry-pdt3

Depositor

James Collins

Article

Cameron et al Nat Biotechnol. 2014 Dec;32(12):1276-81.

Insert

mf-lon

Expression

Bacterial

Promoter

nisA promoter

Available Since

Oct. 5, 2016

Availability

Academic Institutions and Nonprofits only

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FDP-P10B2-URA3

Plasmid

#130875

Purpose

Integration cassette for OrthoRep containing mKate driven by promoter 10B2

Depositor

Chang Liu

Article

Zhong et al ACS Synth Biol. 2018 Dec 21;7(12):2930-2

Insert

mKate

Use

Expression of mkate, leu2(q180*), and ura3 on ort…

Available Since

Aug. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pIYC04

Plasmid

#64741

Purpose

Yeast expression plasmid

Depositor

Jens Nielsen

Article

Chen et al Metab Eng. 2013 Jan;15:48-54. doi: 10.10

Type

Empty backbone

Expression

Yeast

Available Since

June 26, 2015

Availability

Academic Institutions and Nonprofits only

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pEB1-wtGFP

Plasmid

#103978

Purpose

Plasmid encoding wtGFP

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

wtGFP

Use

Low copy

Expression

Bacterial

Promoter

proC

Available Since

Dec. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pHdCas9

Plasmid

#137080

Purpose

Expression of Himar-dCas9 in bacteria

Depositor

Harris Wang

Article

Chen et al CRISPR J. 2019 Dec;2(6):376-394. doi: 10

Insert

Himar-dCas9

Expression

Bacterial

Promoter

pLtetO-1

Available Since

March 3, 2020

Availability

Academic Institutions and Nonprofits only

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pFastbacHTB HA-human PP2A Cα D88N

Plasmid

#132633

Purpose

expresses HA-human PP2A Cα D88N in Hi5 insect cell

Depositor

Wenqing Xu

Article

Cho et al Nature. 2007 Jan 4;445(7123):53-7. doi:

Insert

PP2A Cα D88N

Expression

Insect

Available Since

Oct. 17, 2019

Availability

Academic Institutions and Nonprofits only

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wt 6XHis-SNX9 pET15b

Plasmid

#34690

Depositor

Sandra Schmid

Article

Yarar et al Traffic. 2008 Jan;9(1):133-46. Epub 2007

Insert

Sorting nexin 9

Tags

6xHis

Expression

Bacterial

Available Since

Jan. 31, 2012

Availability

Academic Institutions and Nonprofits only
pRec ΔLacI

Plasmid

#104575

Purpose

Expresses Cas1, Cas2 under PLTetO-1 promoter, and TetR constitutively.

Depositor

Harris Wang

Article

Sheth et al Science. 2017 Dec 15;358(6369):1457-1461

Insert

PTet-cas12, tetR

Use

Synthetic Biology

Available Since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pRec

Plasmid

#104572

Purpose

Expresses Cas1, Cas2 under PLTetO-1 promoter, and TetR and LacI constitutively.

Depositor

Harris Wang

Article

Sheth et al Science. 2017 Dec 15;358(6369):1457-1461

Insert

PTet-cas12, tetR, lacI

Use

Synthetic Biology

Available Since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pAG306-GPD-ccdB chr I

Plasmid

#41894

Depositor

Dan Gottschling

Article

Hughes et al Nature. 2012 Dec 13;492(7428):261-5. doi

Type

Empty backbone

Expression

Yeast

Promoter

GPD

Available Since

Feb. 7, 2013

Availability

Academic Institutions and Nonprofits only

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