We narrowed to 40,023 results for: lat

1. pBluescript II Ks(+)-ZsGreen1-SV40 PolyA-Stop cKit HDR

   Plasmid

   #207135

   Purpose

   DNA sequence source for amplifying an HDR template for mouse cKit knockout and reporter knock-in

   Depositor

   Silvia Monticelli

   Article

   Leoni et al Nat Commun. 2023 Jun 29;14(1):3862. doi:

   Insert

   5'HDR cKit- ZsGreen- sv40(polyA) - 3'HDR cKit (Kit Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1(+)-PKAcat(G1A)-mCherry

   Plasmid

   #181852

   Purpose

   mCherry-tagged PKA catalytic subunit lacking N-terminal myristoylation site; for mammalian expression.

   Depositor

   Jin Zhang

   Article

   Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

   Insert

   PKAcat-mCherry (PRKACA Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Glycine residue immediately after start site in P…

   Promoter

   CMV

   Available Since

   July 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. S+Q111R+N122D

   Plasmid

   #167175

   Purpose

   Expresses the full Leptodactylus latrans Na+K+ATPase with ATP1A1-S variant with Q111R and N122D mutations in Sf9 cells

   Depositor

   Susanne Dobler

   Article

   Mohammadi et al Curr Biol. 2021 Jun 21;31(12):2530-2538.

   Inserts

   ATP1A1-S+Q111R+N122D
   ATP1B1
   

   Use

   Tags

   Expression

   Bacterial and Insect

   Mutation

   Changed Q at 111 to R and changed N at 122 to D

   Promoter

   P10 and PH

   Available Since

   Feb. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. S+10subs

   Plasmid

   #167176

   Purpose

   Expresses the full Leptodactylus latrans Na+K+ATPase with ATP1A1-S variant with 10 R mutations in Sf9 cells

   Depositor

   Susanne Dobler

   Article

   Mohammadi et al Curr Biol. 2021 Jun 21;31(12):2530-2538.

   Inserts

   ATP1A1-S+10R
   ATP1B1
   

   Use

   Tags

   Expression

   Bacterial and Insect

   Mutation

   Changed A at 112 to T; E at 116 to D; I at 135 to…

   Promoter

   P10 and PH

   Available Since

   Feb. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. S+12subs

   Plasmid

   #167177

   Purpose

   Expresses the full Leptodactylus latrans Na+K+ATPase with ATP1A1-S variant with 12 R mutations in Sf9 cells

   Depositor

   Susanne Dobler

   Article

   Mohammadi et al Curr Biol. 2021 Jun 21;31(12):2530-2538.

   Inserts

   ATP1A1-S+12R
   ATP1B1
   

   Use

   Tags

   Expression

   Bacterial and Insect

   Mutation

   Changed Q at 111 to R; A at 112 to T; E at 116to …

   Promoter

   P10 and PH

   Available Since

   Feb. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV_hSyn_HA-TALE(HD)-Tet1S-NLS_2A_EGFP_W3SL

   Plasmid

   #172200

   Purpose

   AAV sequence-specific control vector for TALE-Tet1S-SID4X. Synapsin promoter for neuronal expression. C-terminal, self-cleaving EGFP marker.

   Depositor

   Garrett Kaas

   Article

   Greer et al J Neurosci. 2021 Jan 27;41(4):578-593. d

   Insert

   HA-TALE(HD)-Tet1S-NLS_2A_EGFP_W3SL
   

   Use

   AAV and Mouse Targeting; Tale

   Tags

   HA, NLS, and T2A-EGFP

   Expression

   Mammalian

   Mutation

   transcription regulatory domain removed

   Promoter

   hSyn human synapsin (mammalian neuronal expressio…

   Available Since

   Aug. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. p35S_GFP-EVDinter-GUS

   Plasmid

   #167123

   Purpose

   Plant binary expression vector containing the intron and proximal polyadenylation signal sequences of the Copia93 retroelement EVADE (AT5G17125) between mGFP5 and GUS under CaMV 35S promoter.

   Depositor

   Arturo Marí-Ordóñez, Olivier Voinnet

   Article

   Oberlin et al Genome Res. 2017 Sep;27(9):1549-1562. do

   Insert

   EVADE GAG intron and terminator (EVD_in/ter) (AT5G17125 Mustard Weed)
   

   Use

   Tags

   GUS and mGFP5

   Expression

   Plant

   Mutation

   Promoter

   CaMV 35S

   Available Since

   April 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. DHC1-mIAA17 Hygro

   Plasmid

   #140544

   Purpose

   DHC1 tagging with mIAA7

   Depositor

   Masato Kanemaki

   Article

   Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

   Insert

   DHC1 (DYNC1H1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pPICZ-His6-K2P4.1A-mCherry-StreptagII

   Plasmid

   #158743

   Purpose

   Expresses K2P4.1 isoform A with TEV protease cleavable N- and C-terminal affinity tags.

   Depositor

   Arthur Laganowsky

   Article

   Schrecke et al Nat Chem Biol. 2021 Jan;17(1):89-95. doi

   Insert

   K2P4.1-A (KCNK4 Human)
   

   Use

   Tags

   His6 and mCherry with Streptag II

   Expression

   Yeast

   Mutation

   Residues 1-264 with N-linked glycosylation sites …

   Promoter

   AOX1

   Available Since

   Sept. 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMAL c2x Tral FL-15D

    Plasmid

    #113010

    Purpose

    For bacterial expression of MBP fusion of full-length Drosophila Tral with Phos-mimic mutations

    Depositor

    Terry Orr-Weaver

    Article

    Hara et al Elife. 2018 Feb 26;7. pii: 33150. doi: 1

    Insert

    full length tral (tral Fly)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    15 PNG phosphorylation sites mutated to D

    Promoter

    Available Since

    July 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLuc-Optop38i-lsm

    Plasmid

    #89747

    Purpose

    Expression of light-regulated p38 inhibitor, firefly luciferase-fused, lit-state mutant photosensor (AsLov2Ja.I539E), inhibitor MK3BD3-13F

    Depositor

    Michael Courtney

    Article

    Melero-Fernandez de Mera et al Nat Commun. 2017 May 12;8:15017. doi: 10

    Insert

    Optop38i lit-state mutant
    

    Use

    Luciferase

    Tags

    Firefly luciferase

    Expression

    Mammalian

    Mutation

    I539E mutation of AsLov2Jalpha

    Promoter

    CMV

    Available Since

    Aug. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLuc-OptoJNKi-lsm

    Plasmid

    #89746

    Purpose

    Expression of light-regulated JNK inhibitor, firefly luciferase-fused, lit-state mutant photosensor (AsLov2Ja.I539E), inhibitor JIP11

    Depositor

    Michael Courtney

    Article

    Melero-Fernandez de Mera et al Nat Commun. 2017 May 12;8:15017. doi: 10

    Insert

    OptoJNKi lit-state mutant
    

    Use

    Luciferase

    Tags

    Firefly luciferase

    Expression

    Mammalian

    Mutation

    I539E mutation of AsLov2Jalpha

    Promoter

    CMV

    Available Since

    Aug. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLuc-OptoJNKi-dsm

    Plasmid

    #89745

    Purpose

    Expression of light-regulated JNK inhibitor, firefly luciferase-fused, dark-state mutant photosensor (AsLov2Ja.C450A), inhibitor JIP11

    Depositor

    Michael Courtney

    Article

    Melero-Fernandez de Mera et al Nat Commun. 2017 May 12;8:15017. doi: 10

    Insert

    OptoJNKi dark-state mutant
    

    Use

    Luciferase

    Tags

    Firefly luciferase

    Expression

    Mammalian

    Mutation

    C450A mutation of AsLov2Jalpha

    Promoter

    CMV

    Available Since

    Aug. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pETM6-1D4-mCherry

    Plasmid

    #73423

    Purpose

    Reporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 1D4.

    Depositor

    Mattheos Koffas

    Article

    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

    Insert

    Promoter 1D4 (orthogonal T7-lac variant)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    P1D4 (orthogonal T7-lac variant)

    Available Since

    March 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pETM6-4A6-mCherry

    Plasmid

    #73424

    Purpose

    Reporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 4A6.

    Depositor

    Mattheos Koffas

    Article

    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

    Insert

    Promoter 4A6 (orthogonal T7-lac variant)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    P4A6 (orthogonal T7-lac variant)

    Available Since

    March 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pETM6-1E4-mCherry

    Plasmid

    #73426

    Purpose

    Reporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 1E4.

    Depositor

    Mattheos Koffas

    Article

    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

    Insert

    Promoter 1E4 (orthogonal T7-lac variant)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    P1E4 (orthogonal T7-lac variant)

    Available Since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pETM6-1B6-mCherry

    Plasmid

    #73420

    Purpose

    Reporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 1B6.

    Depositor

    Mattheos Koffas

    Article

    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

    Insert

    Promoter 1B6 (orthogonal T7-lac variant)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    P1B6 (orthogonal T7-lac variant)

    Available Since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pETM6-3H5-mCherry

    Plasmid

    #73419

    Purpose

    Reporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 3H5.

    Depositor

    Mattheos Koffas

    Article

    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

    Insert

    Promoter 3H5 (orthogonal T7-lac variant)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    P3H5 (orthogonal T7-lac variant)

    Available Since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMG023 MBP-Axin 384-518-His (miniAxin)

    Plasmid

    #154012

    Purpose

    Expresses MBP-Axin_384-518-His (human Axin minimal scaffold fragment as a fusion protein with MBP and His- tags) in E.coli

    Depositor

    Jesse Zalatan

    Article

    Gavagan et al Cell Syst. 2020 Jun 24;10(6):515-525.e5.

    Insert

    Axin1 (AXIN1 Human)
    

    Use

    Tags

    His6 and MBP

    Expression

    Bacterial

    Mutation

    381-518 fragment

    Promoter

    Tac

    Available Since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1-Flag-PGC-1alpha1

    Plasmid

    #45501

    Depositor

    Jorge Ruas, Bruce Spiegelman

    Article

    Ruas et al Cell. 2012 Dec 7;151(6):1319-31. doi: 10

    Insert

    PGC-1alpha Isoform1 (Ppargc1a Mouse)
    

    Use

    Tags

    6xHis and Flag

    Expression

    Mammalian

    Mutation

    Q339K relative to NM_008904

    Promoter

    Available Since

    Aug. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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