163,743 results

1. pAAV-CaMKII-GCaMP6f

   Plasmid

   #166022

   Purpose

   Expresses GCaMP6f in CaMKII+ cells

   Depositor

   Xue Han

   Article

   Keaveney et al J Neurosci. 2020 Dec 9;40(50):9576-9588.

   Insert

   GCaMP6f
   

   Use

   AAV and Mouse Targeting

   Tags

   None

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. nuc5-

   Bacterial Strain

   #40804

   Depositor

   George Church

   Article

   Mosberg et al PLoS One. 2012;7(9):e44638. Epub 2012 Se

   Bacterial Resistance

   Ampicillin

   Available since

   Oct. 23, 2012

   Availability

   Academic Institutions and Nonprofits only

3. pCS2-nCas9n-nanos 3’UTR

   Plasmid

   #62542

   Purpose

   expression of a germ-line targeted, codon optimized Cas9 for genome-editing in zebrafish

   Depositor

   Antonio Giraldez

   Article

   Moreno-Mateos et al Nat Methods. 2015 Aug 31. doi: 10.1038/n

   Insert

   nanos 3' UTR (nanos3 Zebrafish)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   3' UTR only

   Promoter

   SP6

   Available since

   July 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRvSAHS1-mEGFP-NLS

   Plasmid

   #205005

   Purpose

   The vector contains 1kbp of the upstream and downstream regions of the sahs1 gene from Ramazzottius varieornatus, with the mEGFP gene with NLS sequence positioned in between.

   Depositor

   Kazuharu Arakawa

   Article

   Tanaka et al Proc Natl Acad Sci U S A. 2023 Jan 31;12

   Insert

   mEGFP-NLS flanked by 1kbp upstream and downstream of the sahs1 gene from Ramazzottius varieornatus
   

   Use

   Tardigrade expression

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRvCAHS3-mEGFP-NLS

   Plasmid

   #205004

   Purpose

   The vector contains 1kbp of the upstream and downstream regions of the cahs3 gene from Ramazzottius varieornatus, with the mEGFP gene with NLS sequence positioned in between.

   Depositor

   Kazuharu Arakawa

   Article

   Tanaka et al Proc Natl Acad Sci U S A. 2023 Jan 31;12

   Insert

   mEGFP-NLS flanked by 1kbp upstream and downstream of the cahs3 gene from Ramazzottius varieornatus
   

   Use

   Tardigrade expression

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. p8.9 NdeltaSB

   Plasmid

   #132929

   Purpose

   Minimal HIV-1 packaging plasmid for gag and pol expression

   Depositor

   Jeremy Luban

   Article

   Berthoux et al J Virol. 2004 Nov;78(21):11739-50. doi:

   Insert

   gag and pol
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGL0_142 [EilR-PjexD]

   Plasmid

   #199026

   Purpose

   Level 0 part

   Depositor

   Charlie Gilbert, Nili Ostrov, Cultivarium Tools

   Article

   Gilbert et al bioRxiv 2023.05.19.541510

   Insert

   EilR-PjexD
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLX208 CMV sTurboID (C)-HA-FRB-ERM

   Plasmid

   #153007

   Purpose

   Expresses split-TurboID C-terminal fragment targeted to endoplasmic reticulum membrane

   Depositor

   Alice Ting

   Article

   Cho et al Proc Natl Acad Sci U S A. 2020 May 18. p

   Insert

   sTurboID (C) (G74)-ERM (Cb5)
   

   Use

   Lentiviral

   Tags

   FRB and HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. hCRISPRi_dual_1_2

   Pooled Library

   #187246

   Purpose

   Ultra-compact, human genome-wide CRISPRi library targeting each gene with a single element encoding a dual sgRNA cassette.

   Depositor

   Marco Jost, Jonathan Weissman

   Article

   Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

   Species

   Synthetic

   Use

   Lentiviral

   Available since

   Sept. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

10. psiCheck2-SV40p-3xFLAG-HSVTKp-mCherry

    Plasmid

    #182377

    Purpose

    Dual expression construct encoding 3xFLAG and mCherry from separate promoters

    Depositor

    Chunghun Lim

    Article

    Lee et al BMC Biol. 2022 Jan 7;20(1):12. doi: 10.1

    Inserts

    3xFlag
    mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    HSV TK promoter and SV40 promoter

    Available since

    March 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHAGE TRE dCas9-KRAB

    Plasmid

    #50917

    Purpose

    Tet-regulatable dCas9-KRAB 2nd generation lentiviral expression vector

    Depositor

    Scot Wolfe, Rene Maehr

    Article

    Kearns et al Development. 2014 Jan;141(1):219-23. doi

    Insert

    dCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    3xHA and KRAB

    Expression

    Mammalian

    Mutation

    D10A, H840A (catalytically inactive)

    Promoter

    TRE

    Available since

    Feb. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBABE-Flag-Cdk9-T186A-IRES-eGFP

    Plasmid

    #28097

    Depositor

    Andrew Rice

    Article

    Dow et al J Cell Physiol. 2010 Jul . 224(1):84-93.

    Insert

    Cdk9 (CDK9 Human)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Contains T186A mutation in Cdk9

    Promoter

    Available since

    April 15, 2011

    Availability

    Academic Institutions and Nonprofits only

13. pDAS12069_U6-pegRNA-mCherry

    Plasmid

    #177180

    Purpose

    pegRNA cloning plasmid with mCherry marker

    Depositor

    Ervin Welker

    Article

    Simon et al Elife. 2022 Feb 23;11. pii: 69504. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Feb. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGMC00025 (aka pCW-dCas9-Blast)

    Plasmid

    #195316

    Purpose

    Dox inducible lentiviral dead Cas9

    Depositor

    Raj Chari

    Insert

    dead Cas9
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    D10A; H840A

    Promoter

    Available since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. BPK3079

    Plasmid

    #78741

    Purpose

    Human expression plasmid for AsCpf1 guide RNA (need to clone in spacer into BsmBI sites)

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nat Biotechnol. 2016 Jun 27. doi: 10.103

    Insert

    AsCpf1 crRNA backbone, without spacer sequence
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    June 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDule-pCNF

    Plasmid

    #85494

    Purpose

    Machinery plasmid expressing permissive pCNF synthetase and cognate amber suppressing tRNA. Incorporates azidoPhenylalanine.

    Depositor

    Ryan Mehl

    Article

    Miyake-Stoner et al Biochemistry. 2009 Jun 30;48(25):5953-62

    Insert

    para-cyanophenylalanine Mj synthetase
    

    Use

    Tags

    None

    Expression

    Bacterial

    Mutation

    Y32L L65V F108W Q109M D158G I159P

    Promoter

    lpp (constitutive)

    Available since

    Jan. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. Tet-O-FUW-Myt1l

    Plasmid

    #27152

    Depositor

    Marius Wernig

    Article

    Vierbuchen et al Nature. 2010 Feb 25. 463(7284):1035-41.

    Insert

    Myt1l (Myt1l Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 4, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV HA hRB-wt

    Plasmid

    #58905

    Purpose

    mammalian expression of wild type human Rb

    Depositor

    Steven Dowdy

    Article

    Narasimha et al Elife. 2014 May 29:e02872. doi: 10.7554/

    Insert

    Rb (RB1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLEX-uORF-HA-birA*-H-Ras(wildtype)-IRES-Puro

    Plasmid

    #120559

    Purpose

    Expresses HA-birA*-H-Ras wildtype fusion protein in mammalian cells & for virus production

    Depositor

    Paul Khavari

    Article

    Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

    Insert

    HRAS (HRAS Human)
    

    Use

    Lentiviral

    Tags

    HA-birA*

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. LentiCROP-PuroR-P2A-GFP-U6

    Plasmid

    #200029

    Purpose

    Lentiviral CROP-seq vector backbone with PuroR-P2A-GFP for cloning DNA Ticker Tapes and U6 for pegRNAs

    Depositor

    Jay Shendure

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    PuroR-P2A-GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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