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NRAS

Plasmid

#55654

Purpose

expression of mouse NRAS

Depositor

Julian Downward

Article

Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

Insert

NRAS (Nras Mouse)

Expression

Bacterial

Promoter

lac

Available Since

Aug. 21, 2014

Availability

Academic Institutions and Nonprofits only
pEX128·gRNA

Plasmid

#187600

Purpose

Template for amplification of gRNA with Cas6 recognition site

Depositor

Pablo Ivan Nikel

Article

Volke et al Nat Commun. 2022 May 31;13(1):3026. doi:

Insert

gRNA template

Promoter

no promoter

Available Since

July 22, 2022

Availability

Academic Institutions and Nonprofits only

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pTmpSm

Plasmid

#176228

Purpose

A template plasmid for amplification of the cI-hok-strA cassette

Depositor

Sergey Sineoky

Article

Bubnov et al Nucleic Acids Res. 2022 Aug 26;50(15):89

Insert

cI of phage lambda, hok of the R1 plasmid, strA

Use

Synthetic Biology

Expression

Bacterial

Available Since

Nov. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pDHt-SK

Plasmid

#63615

Purpose

The multiple cloning site of pGreenII0000 cloned into binary vector pDHt

Depositor

Seogchan Kang

Article

Khang et al Fungal Genet Biol. 2005 Jun;42(6):483-92

Type

Empty backbone

Use

Fungal expression

Available Since

July 27, 2015

Availability

Academic Institutions and Nonprofits only

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pGEX-6P1-Vinculin tail

Plasmid

#107154

Purpose

bacterial expression

Depositor

Tina Izard

Article

Chinthalapudi et al J Cell Biol. 2014 Dec 8;207(5):643-56. d

Insert

Vinculin (VCL Human)

Tags

GST

Expression

Bacterial

Promoter

Ptac

Available Since

March 5, 2018

Availability

Academic Institutions and Nonprofits only

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pN_35S/mEGFP

Plasmid

#132565

Purpose

mEGFP expression regulated by the CaMV35S promoter, co-expressed nourseothricin acetyl transferase selectable marker (nat)

Depositor

Mathias Pribil

Article

Behrendorff et al Physiol Plant. 2020 Feb 25. doi: 10.1111

Insert

mEGFP

Expression

Plant

Promoter

Cauliflower mosaic virus 35S

Available Since

Dec. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-ConVERGD-eGFP-W3SL

Plasmid

#218744

Purpose

Provides AND intersectional (Cre+Flp) expression of eGFP

Depositor

Lindsay Schwarz

Article

Hughes et al bioRxiv. 2023 Feb 8:2023.02.07.527312. d

Insert

eGFP

Use

AAV

Promoter

Ef1a

Available Since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

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pTmpGm

Plasmid

#176229

Purpose

A template plasmid for amplification of the cI-hok-aacC4 cassette

Depositor

Sergey Sineoky

Article

Bubnov et al Nucleic Acids Res. 2022 Aug 26;50(15):89

Insert

cI of phage lambda, hok of the R1 plasmid, aacC4

Use

Synthetic Biology

Expression

Bacterial

Available Since

Nov. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pLV-sgTel

Plasmid

#122659

Purpose

Expresses sgRNA for telomere repeats with fluorescent indicator BFP and puromycin selection marker

Depositor

Clifford Brangwynne

Article

Shin et al Cell. 2018 Nov 29;175(6):1481-1491.e13.

Insert

BFP

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

March 19, 2019

Availability

Academic Institutions and Nonprofits only

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pBS Olfactomedin1

Plasmid

#19225

Depositor

Cliff Tabin

Article

Bandyopadhyay et al Dev Biol. 2008 Sep 1;321(1):162-74. doi:

Insert

Olfactomedin 1 (OLFM1 Chicken)

Expression

Bacterial

Available Since

Sept. 19, 2008

Availability

Academic Institutions and Nonprofits only
pAAV-hSyn-ConVERGD-ConFonvConNon-eGFP-W3SL

Plasmid

#218753

Purpose

Provides AND intersectional (Cre+Flp+vCre+Vika) expression of eGFP

Depositor

Lindsay Schwarz

Article

Hughes et al bioRxiv. 2023 Feb 8:2023.02.07.527312. d

Insert

eGFP

Use

AAV

Promoter

hSyn

Available Since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

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pICH51288

Plasmid

#50269

Depositor

Sylvestre Marillonnet, Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

promoter (double), 35s (Cauliflower Mosaic Virus) + 5'UTR, omega (Tobacco Mosaic Virus)

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available Since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pGG-TurboID

Plasmid

#209391

Purpose

Transiently expressing TurboID in planta

Depositor

Rosa Lozano-Durán

Article

Tan et al J Integr Plant Biol. 2024 Jan 31. doi: 1

Insert

3XHA-TurboID

Expression

Plant

Mutation

TurboID gene sequence C249A

Promoter

Cauliflower mosaic virus (CaMV) 35S promoter

Available Since

Jan. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pUC_sgRNA

Plasmid

#68710

Purpose

Contains the sgRNA backbone sequence (tracrRNA, 82 bp) and is used as DNA template to amplify a specific sgRNA using a forward primer with the protospacer sequence for gene targeting.

Depositor

Roberto Docampo

Article

Lander et al MBio. 2015 Jul 21;6(4). pii: e01012-15.

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Available Since

Sept. 14, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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MRAS

Plasmid

#55661

Purpose

expression of mouse MRAS

Depositor

Julian Downward

Article

Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

Insert

MRAS (Mras Mouse)

Expression

Bacterial

Promoter

lac

Available Since

Aug. 21, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3.1(+) CircRNA Mini Vector-ZKSCAN1 nt 548-1047

Plasmid

#60649

Purpose

Expresses a miniature version of the ZKSCAN1 circular RNA

Depositor

Jeremy Wilusz

Article

Liang et al Genes Dev. 2014 Oct 3. pii: gad.251926.1

Insert

ZKSCAN1 (ZKSCAN1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 27, 2014

Availability

Academic Institutions and Nonprofits only

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pSumo-His6-SUMO-AtTPR1(1-209)

Plasmid

#177858

Purpose

Bacterial Expression of TPR1

Depositor

Karsten Melcher

Article

Ke et al Sci Adv. 2015 Jul 24;1(6):e1500107. doi:

Insert

TPR1 (TPR1 Mustard Weed)

Tags

6xHis-SUMO

Expression

Bacterial

Available Since

Dec. 10, 2021

Availability

Academic Institutions and Nonprofits only

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Rac1

Plasmid

#55692

Purpose

Expression of mouse Rac1

Depositor

Julian Downward

Article

Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

Insert

Rac1 (Rac1 Mouse)

Expression

Bacterial

Promoter

lac

Available Since

Sept. 30, 2014

Availability

Academic Institutions and Nonprofits only
pCMV_mCherry_2A_HA_2xMS2-VP64

Plasmid

#68419

Purpose

Transient expression of humanized MS2 (V75E/A81G), fused to the VP64 transcrption activator, in mammalian cells, under a CMV promoter

Depositor

John Rinn

Article

Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

Insert

MS2

Use

Synthetic Biology

Tags

HA and VP64

Expression

Mammalian

Mutation

MS2: V75E/A81G

Promoter

CMV

Available Since

Sept. 21, 2015

Availability

Academic Institutions and Nonprofits only

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ciCas9_pcDNA5

Plasmid

#100550

Purpose

Expresses ciCas9 in mammalian cells. Can be used to generate Flp-In and Flp-In T-REx stables.

Depositor

Dustin Maly

Article

Rose et al Nat Methods. 2017 Jul 24. doi: 10.1038/n

Insert

ciCas9

Use

CRISPR; Frt, flp-in

Tags

FLAG

Expression

Mammalian

Available Since

Sept. 22, 2017

Availability

Academic Institutions and Nonprofits only

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