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pcDNA3.1-mCherry

Plasmid

#128744

Purpose

Expresses mCherry in mammalian cells

Depositor

David Bartel

Article

Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

Type

Empty backbone

Expression

Mammalian

Available Since

Aug. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pET28c-F30-2xdBroccoli

Plasmid

#66843

Purpose

Expresses two dimeric Broccoli aptamers in the F30 scaffold in bacterial cells

Depositor

Samie Jaffrey

Article

Filonov et al Curr Protoc Chem Biol. 2016 Mar 16;8(1):

Insert

Two dimeric Broccoli aptamers in the F30 scaffold

Expression

Bacterial

Promoter

T7

Available Since

July 13, 2015

Availability

Academic Institutions and Nonprofits only

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CMV-Tol2 transposase

Plasmid

#158774

Purpose

Expression of Tol2 transposase

Depositor

Hugo Snippert

Article

Heinz et al STAR Protoc. 2020 Oct 8;1(3):100126. doi

Insert

Tol2 tranposase

Expression

Mammalian

Mutation

Tol2 codon usage frequency well suitable for muri…

Available Since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-MCS2

Plasmid

#46954

Purpose

Cloning plasmid for the generation of Knock-In in human cells using rAAV.

Depositor

Steve Jackson

Article

Britton et al J Cell Biol. 2013 Jul 29.

Type

Empty backbone

Use

AAV

Available Since

Sept. 19, 2013

Availability

Academic Institutions and Nonprofits only

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pSFV3-lacZ

Plasmid

#92074

Purpose

This is an expression plasmid for production of recombinant genomic SFV RNA encoding beta-galactosidase.

Depositor

Henrik Garoff

Article

Liljestrom et al Biotechnology (N Y). 1991 Dec;9(12):1356

Insert

lacZ

Use

Lacz expression plasmid, for run-off transcriptio…

Available Since

June 27, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA-GFP

Plasmid

#160697

Purpose

Expresses GFP in mammalian cells

Depositor

Christine Mayr

Article

Lee et al Mol Cell. 2019 May 16;74(4):701-712.e9.

Insert

GFP

Expression

Mammalian

Available Since

Nov. 17, 2020

Availability

Academic Institutions and Nonprofits only

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poGFP1

Plasmid

#154127

Purpose

Expresses orthogonal gfp gene in bacterial cells

Depositor

Alexander Mankin

Article

Aleksashin et al Nat Commun. 2020 Apr 20;11(1):1858. doi:

Insert

orthogonal gfp

Use

Synthetic Biology

Expression

Bacterial

Available Since

Oct. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pFp10

Plasmid

#170460

Purpose

EarlyBac insect cell expression system

Depositor

Hiro Furukawa

Article

Chou et al Cell. 2020 Jul 23;182(2):357-371.e13. do

Insert

none

Expression

Insect

Promoter

dHsp70

Available Since

June 23, 2021

Availability

Academic Institutions and Nonprofits only

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MSCV-CMV-DsRed-IRES-d1EGFP

Plasmid

#41942

Purpose

Retroviral expression of DsRed in mammalian cells

Depositor

Stephen Elledge

Article

Yen et al Science. 2008 Nov 7;322(5903):918-23. do

Insert

DsRed

Use

Retroviral

Expression

Mammalian

Promoter

TRE

Available Since

May 2, 2013

Availability

Academic Institutions and Nonprofits only

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pHR_Gal4UAS_dGFP_PGK_iRFP (pLZA042)

Plasmid

#203908

Purpose

Encodes dGFP downstream UAS promoter that's responsive to Gal4-VP64 activity in mammalian cells

Depositor

Jared Toettcher

Article

Zhu et al Nat Commun. 2023 Jun 2;14(1):3185. doi:

Insert

Gal4UAS_dGFP_PGK_iRFP

Expression

Mammalian

Promoter

5xUAS and PGK

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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Construct 32 - NFKBRp_mKate2_2xNLS_p2a-puroR

Plasmid

#82024

Purpose

Lentiviral construct for building stable cell lines expressing mKate2 in the presence of TNFa via puromycin selection

Depositor

Timothy Lu

Article

Perli et al Science. 2016 Aug 18. pii: aag0511.

Insert

mKate2-2xNLS

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Available Since

Sept. 14, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_ionRD

Plasmid

#172931

Purpose

Ionic strength FRET sensor with mCerulean3 and mCitrine for mammalian cell expression.

Depositor

Arnold Boersma

Article

Liu et al ACS Chem Biol. 2017 Oct 20;12(10):2510-2

Insert

IonicStrengthSensorRD

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 17, 2021

Availability

Academic Institutions and Nonprofits only

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MSCV-CMV-DsRed-IRES-d24EGFP

Plasmid

#41944

Purpose

Retroviral expression of DsRed in mammalian cells

Depositor

Stephen Elledge

Article

Yen et al Science. 2008 Nov 7;322(5903):918-23. do

Type

Empty backbone

Use

Retroviral

Expression

Mammalian

Promoter

TRE

Available Since

May 2, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_ionRE

Plasmid

#172932

Purpose

Ionic strength FRET sensor with mCerulean3 and mCitrine for mammalian cell expression.

Depositor

Arnold Boersma

Article

Liu et al ACS Chem Biol. 2017 Oct 20;12(10):2510-2

Insert

IonicStrengthSensorRE

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 10, 2021

Availability

Academic Institutions and Nonprofits only

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Construct 28 -hUBCp_TetR_p2a_LacI_p2a_ZeoR

Plasmid

#81253

Purpose

Lentiviral construct for building stable cell lines expressing TetR and LacI via Zeocin selection

Depositor

Timothy Lu

Article

Perli et al Science. 2016 Aug 18. pii: aag0511.

Insert

TetR and LacI

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Available Since

Sept. 20, 2016

Availability

Academic Institutions and Nonprofits only

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pSPLp10

Plasmid

#170462

Purpose

EarlyBac insect cell expression system

Depositor

Hiro Furukawa

Article

Chou et al Cell. 2020 Jul 23;182(2):357-371.e13. do

Insert

New vector

Expression

Insect

Promoter

dHsp70

Available Since

Aug. 3, 2021

Availability

Academic Institutions and Nonprofits only

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ptdTomato-SMCR8

Plasmid

#83438

Purpose

Expresses SMCR8-tdTomato in mammalian cells

Depositor

Shawn Ferguson

Article

Amick et al Mol Biol Cell. 2016 Aug 24. pii: mbc.E16

Insert

Human SMCR8 (SMCR8 Human)

Tags

tdTomato

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pICE

Plasmid

#46960

Purpose

Plasmid for cloning and constitutive or doxycycline-inducible expression of cDNAs. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.

Depositor

Steve Jackson

Article

Britton et al J Cell Biol. 2013 Jul 29.

Type

Empty backbone

Expression

Mammalian

Available Since

Aug. 20, 2013

Availability

Academic Institutions and Nonprofits only

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MSCV-CMV-DsRed-IRES-d4EGFP

Plasmid

#41943

Purpose

Retroviral expression of DsRed in mammalian cells

Depositor

Stephen Elledge

Article

Yen et al Science. 2008 Nov 7;322(5903):918-23. do

Insert

DsRed

Use

Retroviral

Expression

Mammalian

Promoter

TRE

Available Since

July 15, 2014

Availability

Academic Institutions and Nonprofits only

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pFastBac1-Nucleocapsid

Plasmid

#179696

Purpose

Expresses H6 tagged SARS CoV-2 nucleocapsid in Sf9 cells

Depositor

Mark Arbing

Insert

SARS-CoV-2 Nucleocapsid (N protein) (N Severe acute respiratory syndrome coronavirus 2)

Tags

His6-TEV protease site

Expression

Insect

Promoter

polyhedrin

Available Since

Jan. 14, 2022

Availability

Academic Institutions and Nonprofits only

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