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pENTR4-V5-LUC (w468-1)

Plasmid

#19135

Purpose

Gateway entry vector, V5-Luciferase

Depositor

Eric Campeau, Paul Kaufman

Publication

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Insert

Firefly Luciferase

Use

Gateway Cloning

Tags

V5

Available since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pET28a-pro-sumo1

Plasmid

#25101

Depositor

Guy Salvesen

Publication

Mikolajczyk et al J Biol Chem. 2007 Sep 7. 282(36):26217-2

Insert

pro-SUMO1 (SUMO1 Human)

Tags

His

Expression

Bacterial

Mutation

Changed Serine 2 to Alanine

Available since

June 22, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
BAT-gal

Plasmid

#20889

Depositor

Stefano Piccolo

Publication

Maretto et al Proc Natl Acad Sci U S A. 2003 Mar 18. 1

Insert

7XTCF binding sites, Siamois minimal promoter, NLS-lacz

Use

Mouse Targeting

Expression

Mammalian

Mutation

7 multimerized TCF-binding elements cloned upstre…

Available since

May 7, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles
pcDNA5/FRT/TO V5 DNAJA1

Plasmid

#19518

Depositor

Harm Kampinga

Publication

Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

Insert

DnaJ (Hsp40) homolog, subfamily A, member 1 (DNAJA1 Human)

Tags

V5

Expression

Mammalian

Available since

Oct. 6, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pcDNA5/FRT_mEGFP-SNAP-tag2

Plasmid

#226513

Purpose

Mamalian cell expression of cytosolic mEGFP-SNAP-tag2

Depositor

Kai Johnsson

Publication

Kühn et al Nat Chem Biol. 2025 Jul 3. doi: 10.1038/

Insert

mEGFP-SNAP-tag2

Expression

Mammalian

Available since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pSF464

Plasmid

#167672

Purpose

Intermediate vector for four gRNAs using GoldenGate, contains ccdB and attL1-L2 sites for Gateway

Depositor

Niko Geldner

Publication

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

attL1-ccdB-attL2

Use

CRISPR

Available since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pR26

Plasmid

#127372

Purpose

Allows for doxycycline-inducible gene expression from the murine ROSA26 safe harbor locus upon CRISPR/Cas9-mediated genomic insertion and stable selection.

Depositor

Lance Miller

Publication

Routh et al Front Immunol. 2020 Feb 13;11:57. doi: 1

Type

Empty backbone

Use

CRISPR and Mouse Targeting

Promoter

tight TRE promoter

Available since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pMpGWB103

Plasmid

#68557

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Publication

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Expression

Plant

Promoter

MpEF1alpha promoter

Available since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDONR223-NEK2

Plasmid

#23658

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

NEK2 (NEK2 Human)

Use

Gateway Cloning

Mutation

Insert encodes truncated protein relative to NP_0…

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
PGK plasmid

Plasmid

#169744

Purpose

Unregulated "open-loop" PGK promoter plasmid. This plasmid encodes eGFP to report circuit output levels.

Depositor

Francois St-Pierre

Publication

Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:

Insert

eGFP

Use

Synthetic Biology

Expression

Mammalian

Promoter

PGK promoter

Available since

June 9, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pTOX8

Plasmid

#127453

Purpose

Encodes MqsR toxin and tsPurple chromoprotein. CAM-R

Depositor

Matthew Waldor

Publication

Lazarus et al Appl Environ Microbiol. 2019 Jun 14. pii

Inserts

mqsR toxin
tsPurple chromoprotein

Expression

Bacterial

Mutation

Codon optimized from protein sequence in FPbase

Promoter

apFAB46 and pRha

Available since

July 24, 2019

Availability

Academic Institutions and Nonprofits only

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pAG305GAL-ccdB

Plasmid

#14137

Depositor

Susan Lindquist

Publication

Alberti et al Yeast. 2007 Oct . 24(10):913-9.

Type

Empty backbone

Use

Gateway Cloning

Expression

Yeast

Available since

March 16, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pAG415GPD-ccdB-HA

Plasmid

#14242

Depositor

Susan Lindquist

Publication

Alberti et al Yeast. 2007 Oct . 24(10):913-9.

Type

Empty backbone

Use

Gateway Cloning

Tags

3HA

Expression

Yeast

Available since

June 18, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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Cys-3HB-SPYcatcher

Plasmid

#214838

Purpose

This plasmid encodes a protein (Cys-3HB-SPYcatcher) that is used for generating functionalized magnetic beads for MagIC-cryo-EM, a method for single-particle cryo-EM analysis on magnetic beads.

Depositor

Hironori Funabiki

Publication

Arimura et al eLife. 2025 May 20;13:RP103486. doi: 10.

Insert

Cys-3HB-SPYcatcher

Tags

His-TEV-SUMO-

Expression

Bacterial

Promoter

T5

Available since

March 14, 2024

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pLEX307-TMPRSS2-G418

Plasmid

#158459

Purpose

Constitutive lentiviral expression of human TMPRSS2.

Depositor

Alejandro Chavez, Sho Iketani

Insert

TMPRSS2 (TMPRSS2 Human)

Use

Lentiviral

Expression

Mammalian

Available since

Sept. 17, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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UAS:Zebrabow-B

Plasmid

#118216

Purpose

For broad gal4 inducible expression of Brainbow in zebrafish

Depositor

Albert Pan, Alex Schier

Publication

Pan et al Development. 2013 Jul;140(13):2835-46. d

Insert

Brainbow 1.0 L

Use

Zebrafish expression

Available since

Nov. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pAJM.771

Plasmid

#108534

Purpose

NahRAM +PSalTTC-YFP Reporter. Sal sensor for use in non-Marionette strain.

Depositor

Christopher Voigt

Publication

Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

Insert

NahRAM + PSalTTC-YFP

Expression

Bacterial

Mutation

NahR: I25V P72H V118A R248C

Available since

July 6, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pLD-puro-CnVA

Plasmid

#24587

Depositor

Jason Moffat

Publication

Mak et al Mol Cell Proteomics. 2010 May;9(5):811-2

Insert

Gateway(TM) cassette

Use

Lentiviral

Tags

VA

Expression

Mammalian

Available since

July 14, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pKRaION2-N1

Plasmid

#177802

Purpose

Mammalian expression of green fluorescent potassium indicator KRaION2

Depositor

Edward Boyden

Publication

Torres Cabán et al ACS Sens. 2022 May 27;7(5):1336-1346. do

Insert

KRaION2

Expression

Mammalian

Promoter

CMV

Available since

Jan. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pMZ283

Plasmid

#52224

Purpose

Expression of sgRNA precursor with CspCI placeholder for target cloning (see comments).

Depositor

Mikel Zaratiegui

Publication

Jacobs et al Nat Commun. 2014 Oct 29;5:5344. doi: 10.

Insert

rrk1-sgRNA

Use

CRISPR

Expression

Yeast

Promoter

rrk1

Available since

Oct. 29, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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