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pCMV Flag cycT2b (P#1718)

Plasmid

#14599

Depositor

Matija Peterlin

Article

Kurosu et al Gene. 2004 Dec 8. 343(1):173-9.

Insert

cycT2 (CCNT2 Human)

Tags

Flag

Expression

Mammalian

Mutation

A137T compared to NP_490595.1

Available Since

June 30, 2008

Availability

Academic Institutions and Nonprofits only

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pShuttle-CMV-EGFP-C

Plasmid

#13887

Depositor

Lorene Lanier

Article

Strasser et al Neuron. 2004 Jul 8. 43(1):81-94.

Insert

C domain of N-WASP (WASL Bovine)

Use

Adenoviral

Tags

EGFP

Mutation

The BglII site in the MCS of pShuttle-CMV was fil…

Available Since

Feb. 23, 2007

Availability

Academic Institutions and Nonprofits only

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pCDNA3 Flag Jnk2a2

Plasmid

#13801

Depositor

Roger Davis

Article

Sluss et al Mol Cell Biol. 1994 Dec . 14(12):8376-84

Insert

Jnk2a2 (MAPK9 Human)

Tags

Flag

Expression

Mammalian

Available Since

Feb. 22, 2007

Availability

Academic Institutions and Nonprofits only
1342 pSG5L HA FKHRL1 AAA

Plasmid

#10711

Depositor

William Sellers

Article

Ramaswamy et al Cancer Cell. 2002 Jul . 2(1):81-91.

Insert

FKHRL1 AAA (FOXO3 Human)

Tags

HA

Expression

Mammalian

Mutation

AAA mutant (see map)

Available Since

April 4, 2006

Availability

Academic Institutions and Nonprofits only
584 pSG5L HA RB 661W

Plasmid

#10731

Depositor

William Sellers

Article

Sellers et al Genes Dev. 1998 Jan 1. 12(1):95-106.

Insert

RB 661W (RB1 Human)

Tags

HA

Expression

Mammalian

Mutation

661W

Available Since

Sept. 27, 2005

Availability

Academic Institutions and Nonprofits only
pTetR-LasR-pLuxR-GFP

Plasmid

#171159

Purpose

Sensing Device with Reporter protein

Depositor

Matthew Chang

Article

Saeidi et al Mol Syst Biol. 2011 Aug 16;7:521. doi: 1

Inserts

lasR
gfp

Use

Synthetic Biology

Expression

Bacterial

Promoter

pLux and pTet

Available Since

July 15, 2021

Availability

Academic Institutions and Nonprofits only

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pMDJ20 - pEXP [Psmu-1 | Cre Recombinase (PATCs) | smu-1 UTR]

Plasmid

#159808

Purpose

Optimized enzyme for genome editing in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

pEXP [Psmu-1 | Cre Recombinase (PATCs) | smu-1 UTR]

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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mCherry-FKBP-PI4KAC1001(D1957A)

Plasmid

#139312

Purpose

Recruitable PI4KA kinase-dead catalytic fragment

Depositor

Gerry Hammond

Article

Zewe et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

mCherry: FKBP1A(3-108): GGSA]4GG:PI4KA(1102-2103) (PI4KA Human)

Expression

Mammalian

Mutation

D1957A, G1986D

Available Since

April 8, 2020

Availability

Academic Institutions and Nonprofits only

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MOM603

Plasmid

#133242

Purpose

expresses full-length human KIF1A fused with mScarlet and StrepII tag in insect cells

Depositor

Shinsuke Niwa

Article

Chiba et al Proc Natl Acad Sci U S A. 2019 Aug 27. p

Insert

kif1a (KIF1A Human)

Tags

StrepII tag and mScarlet

Expression

Insect

Promoter

polyhedrin promoter

Available Since

Jan. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pIDS-RESTwt full-length

Plasmid

#109161

Purpose

Encodes human full-length REST/NRSF to be expressed in a baculovirus/insect cell expression system

Depositor

Monika Golas

Article

Inui et al Protein Sci. 2017 May;26(5):997-1011. do

Insert

human full-length REST/NRSF (REST Human)

Expression

Insect

Promoter

p10

Available Since

Jan. 11, 2019

Availability

Academic Institutions and Nonprofits only

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p-mCherry-C1-hCRACR2A-isoform-a

Plasmid

#79593

Purpose

Transient expression

Depositor

Yousang Gwack

Article

Srikanth et al Sci Signal. 2016 Mar 22;9(420):ra31. doi

Insert

CRACR2A-isoform-a (CRACR2A Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

July 29, 2016

Availability

Academic Institutions and Nonprofits only
pCMV0-SMAC-HA-eGFP

Plasmid

#67486

Purpose

Targets HA-eGFP to mitochondrial intermembrane space (IMS)

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

SMAC-HA-eGFP (DIABLO Human)

Expression

Mammalian

Promoter

CMV

Available Since

May 9, 2016

Availability

Academic Institutions and Nonprofits only
p420-3

Plasmid

#71257

Purpose

luciferase reporter with 3 copies of the GM420 NFAT-AP-1 site in front of hGM-CSF -55 to +28 minimal promoter

Depositor

Peter Cockerill

Article

Johnson et al Mol Cell Biol. 2004 Sep;24(18):7914-30.

Insert

3 copies of the GM420 NFAT-AP-1 site (CSF2 Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Promoter

GM-CSF minimal

Available Since

Dec. 4, 2015

Availability

Academic Institutions and Nonprofits only

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AR-V7-pcw107-V5

Plasmid

#64636

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

David Sabatini, Kris Wood

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

AR (transcript variant 1, splice isoform) (AR Human)

Use

Lentiviral

Tags

V5

Mutation

splice isoform *

Promoter

PGK

Available Since

Dec. 1, 2015

Availability

Academic Institutions and Nonprofits only
pAC-DELTA

Plasmid

#53273

Purpose

Contains crtE, crtB, and crtI genes of Erwinia herbicola (Pantoea agglomerans) Eho10, together with the lcyE gene of Arabidopsis thaliana and thereby produces delta-carotene in E. coli

Depositor

Francis X Cunningham Jr

Article

Sun et al J Biol Chem. 1996 Oct 4;271(40):24349-52

Insert

lcyE (LUT2 Mustard Weed)

Use

Low copy number bacterial cloning vector

Promoter

Trc

Available Since

Jan. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pJN105-Hygro-GFP

Plasmid

#41883

Depositor

Howard Steinman

Article

Bandyopadhyay et al Infect Immun. 2004 Aug;72(8):4541-51.

Insert

Ptac::GFP-mut-2

Use

Bacterial broad host range vector

Expression

Bacterial

Mutation

GFP-mut-2 is GFP with Ser65Ala, Val68Leu, and Ser…

Promoter

Ptac

Available Since

June 11, 2013

Availability

Academic Institutions and Nonprofits only
pGolf-ISH

Plasmid

#15853

Depositor

Peter Mombaerts

Article

Hirota et al Mol Cell Neurosci. 2007 Apr . 34(4):679-

Insert

Guanine Nucleotide Binding Protein, Alpha Stimulating, Olfactory type (Gnal Mouse)

Use

Cloning vector

Mutation

F199S

Available Since

March 31, 2009

Availability

Academic Institutions and Nonprofits only
pcDNA3.1 myc his Su(fu) (CT#768)

Plasmid

#13855

Depositor

Cliff Tabin

Article

Pearse et al Dev Biol. 1999 Aug 15. 212(2):323-36.

Insert

Su(fu) (SUFU Chicken)

Tags

his and myc

Expression

Mammalian

Mutation

full ORF

Available Since

Feb. 15, 2007

Availability

Academic Institutions and Nonprofits only
pLV hU6-gRNA hUbC-dSaCas9-KRAB-T2A-Thy1.1

Plasmid

#194278

Purpose

Expresses gRNA and dSaCas9-KRAB from lentiviral vector

Depositor

Charles Gersbach

Article

McCutcheon et al bioRxiv. 2023 May 1:2023.05.01.538906. d

Inserts

humanized dSaCas9 KRAB T2A Thy1.1
gRNA

Use

CRISPR and Lentiviral

Tags

HA

Expression

Mammalian

Promoter

hU6 and hUbC

Available Since

Jan. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pLV-EF1A-LivePAR-Hygro

Plasmid

#176063

Purpose

EGFP fused to the C-terminus of a WWE domain & a hygromycin resistance cassette

Depositor

Robert Sobol

Article

Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

Insert

LivePAR

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

EF1A

Available Since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

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