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pDONR223_DOK1_p.P419S

Plasmid

#82768

Purpose

Gateway Donor vector containing DOK1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

DOK1 (DOK1 Human)

Use

Gateway entry vector

Mutation

P419S

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_DOK1_p.V393L

Plasmid

#82774

Purpose

Gateway Donor vector containing DOK1, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

DOK1 (DOK1 Human)

Use

Gateway entry vector

Mutation

V393L

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_GPR137B_p.C212S

Plasmid

#82757

Purpose

Gateway Donor vector containing GPR137B, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

GPR137B (GPR137B Human)

Use

Gateway entry vector

Mutation

C212S

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_DOK1_p.S460I

Plasmid

#82766

Purpose

Gateway Donor vector containing DOK1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

DOK1 (DOK1 Human)

Use

Gateway entry vector

Mutation

S460I

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_FAM173B_p.R187P

Plasmid

#82748

Purpose

Gateway Donor vector containing FAM173B, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

FAM173B (FAM173B Human)

Use

Gateway entry vector

Mutation

T75M; R187P

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_GPR137B_p.Y135N

Plasmid

#82752

Purpose

Gateway Donor vector containing GPR137B, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

GPR137B (GPR137B Human)

Use

Gateway entry vector

Mutation

Y135N

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_CCND1_p.E172K

Plasmid

#82733

Purpose

Gateway Donor vector containing CCND1, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

CCND1 (CCND1 Human)

Use

Gateway entry vector

Mutation

E172K

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_SERPINB5_p.I159S

Plasmid

#82734

Purpose

Gateway Donor vector containing SERPINB5, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

SERPINB5 (SERPINB5 Human)

Use

Gateway entry vector

Mutation

I159S; S176P; p.190_192delTDTins25amino acids; p.…

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_SERPINB5_p.D141A

Plasmid

#82735

Purpose

Gateway Donor vector containing SERPINB5, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

SERPINB5 (SERPINB5 Human)

Use

Gateway entry vector

Mutation

p. D141A; p. 176S>P; p.190_192delTDTins25amino…

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_SERPINB5_p.A7T

Plasmid

#82736

Purpose

Gateway Donor vector containing SERPINB5, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

SERPINB5 (SERPINB5 Human)

Use

Gateway entry vector

Mutation

p. A7T; p.176S>P; p. 190_192delTDTins25; p.194…

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_SERPINB5_p.A42F

Plasmid

#82738

Purpose

Gateway Donor vector containing SERPINB5, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

SERPINB5 (SERPINB5 Human)

Use

Gateway entry vector

Mutation

p.A42F; p.176S>P; p.190_192delTDTins25; p.194_…

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_SERPINB5_p.A165T

Plasmid

#82741

Purpose

Gateway Donor vector containing SERPINB5, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

SERPINB5 (SERPINB5 Human)

Use

Gateway entry vector

Mutation

p. A165T; p.176S>P; p.190_192delTDTins25; p.19…

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_HAVCR2_p.P6S

Plasmid

#82742

Purpose

Gateway Donor vector containing HAVCR2, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

HAVCR2 (HAVCR2 Human)

Use

Gateway entry vector

Mutation

p.P6S; p.R140L

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pET-Duet-ECHA(37-763) K351R-FLAG (no His-tag)

Plasmid

#96888

Purpose

expresses-flag tagged mouse ECHA(37-768) K351R in E.coli cells (no His-tag)

Depositor

Hening Lin

Article

Sadhukhan et al Proc Natl Acad Sci U S A. 2016 Apr 19;11

Insert

Trifunctional enzyme subunit alpha, mitochondrial (Hadha Mouse)

Tags

FLAG-tagged

Expression

Bacterial

Mutation

deleted amino acid 1-36, mutated lysine 351 to ar…

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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pU6-pegRNA-LRRK2-G2019S-3b

Plasmid

#180433

Purpose

Transiently expressing a pegRNA to introduce LRRK2-G2019S mutation in human cells

Depositor

Frank Soldner

Article

Li et al Elife. 2022 Sep 7;11:e79208. doi: 10.755

Insert

ncRNA targeting LRRK2-G2019S (LRRK2 Human)

Use

CRISPR

Expression

Mammalian

Mutation

LRRK2-G2019S

Promoter

U6

Availability

Academic Institutions and Nonprofits only

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pU6-pegRNA-LRRK2-G2019S-3c

Plasmid

#180434

Purpose

Transiently expressing a pegRNA to introduce LRRK2-G2019S mutation in human cells

Depositor

Frank Soldner

Article

Li et al Elife. 2022 Sep 7;11:e79208. doi: 10.755

Insert

ncRNA targeting LRRK2-G2019S (LRRK2 Human)

Use

CRISPR

Expression

Mammalian

Mutation

LRRK2-G2019S

Promoter

U6

Availability

Academic Institutions and Nonprofits only

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pU6-pegRNA-LRRK2-G2019S-3d

Plasmid

#180435

Purpose

Transiently expressing a pegRNA to introduce LRRK2-G2019S mutation in human cells

Depositor

Frank Soldner

Article

Li et al Elife. 2022 Sep 7;11:e79208. doi: 10.755

Insert

ncRNA targeting LRRK2-G2019S (LRRK2 Human)

Use

CRISPR

Expression

Mammalian

Mutation

LRRK2-G2019S

Promoter

U6

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-ABE8e-nSpRY-P2A-EGFP (KAC1069)

Plasmid

#185912

Purpose

pCMV and pT7 plasmid encoding human codon optimized ABE8e A-to-G base editor with nicakse SpRY(D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) and P2A-EGFP

Depositor

Benjamin Kleinstiver

Article

Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

Insert

human codon optimized ABE8e-nSpRY-P2A-EGFP

Use

In vitro transcription; t7 promoter

Tags

BPNLS and BPNLS-3xFLAG-P2A-EGFP

Expression

Mammalian

Mutation

nSpRY=D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219…

Promoter

CMV and T7

Available Since

July 20, 2022

Availability

Academic Institutions and Nonprofits only

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dRED

Plasmid

#139836

Purpose

Expresses dRNase H1-EGFP-dCas9 fusion protein in human cells

Depositor

Karim Mekhail

Article

Abraham et al Nature. 2020 Sep;585(7824):298-302. doi:

Insert

dRNase H1-EGFP-dCas9 (RNASEH1 dRNase H1 (H. sapiens), dCas9 (S. pyogenes))

Expression

Mammalian

Mutation

RNase H1 protein contains D210N mutation. dCas9 …

Promoter

tet-inducible CMV

Available Since

Sept. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pLKO-Tet-On-YAP1-shRNA1

Plasmid

#176846

Purpose

lentiviral vector for doxycycline-inducible human YAP1 knockdown

Depositor

Roland Friedel

Article

Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

Insert

YAP1-Sh1 (YAP1 Human)

Use

Lentiviral

Available Since

Nov. 5, 2021

Availability

Academic Institutions and Nonprofits only

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