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pTS2401-Tier1-PhCMV-HygroR

Plasmid

#169493

Purpose

Tier-1 vector encoding PhCMV-driven hygromycin resistance gene HygroR (PhCMV-HygroR-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven hph

Expression

Mammalian

Promoter

PhCMV

Available Since

June 14, 2022

Availability

Academic Institutions and Nonprofits only

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pTS1021-Tier1-PhCMV-PuroR

Plasmid

#169492

Purpose

Tier-1 vector encoding PhCMV-driven marker for puromycin selection (PhCMV-PuroR-pA)

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven puromycin N-acetyl-transferase

Expression

Mammalian

Promoter

PhCMV

Available Since

June 14, 2022

Availability

Academic Institutions and Nonprofits only

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pTS1018-Tier1-PhCMV-p2A

Plasmid

#169491

Purpose

Tier-1 vector encoding a self-cleaving peptides p2a sequence (PhCMV-p2A-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven 2A sequence of porcine teschovirus-1

Expression

Mammalian

Promoter

PhCMV

Available Since

June 14, 2022

Availability

Academic Institutions and Nonprofits only

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pVH17-Tier1-PhCMV-myr

Plasmid

#169489

Purpose

Tier-1 vector encoding PhCMV-driven myristoylation (myr) signal peptide expression (PhCMV-myr-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven myrisoylation signal

Expression

Mammalian

Promoter

PhCMV

Available Since

June 14, 2022

Availability

Academic Institutions and Nonprofits only

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pTS2409-Tier1-PhCMV-FURINsite

Plasmid

#169488

Purpose

Tier-1 vector encoding PhCMV-controlled FURIN protease recognition site (PhCMV-FURINsite-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven Furin cleavage site

Expression

Mammalian

Promoter

PhCMV

Available Since

June 14, 2022

Availability

Academic Institutions and Nonprofits only

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pTS2379-Tier1-PhCMV-PEST

Plasmid

#169484

Purpose

Tier-1 vector encoding PhCMV-driven PEST tag for destabilization of proteins (PhCMV-PEST-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven PEST degradation tag

Expression

Mammalian

Promoter

PhCMV

Available Since

June 14, 2022

Availability

Academic Institutions and Nonprofits only

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pTS2415-Tier1-PPGK-1xEAAAK

Plasmid

#169482

Purpose

Tier-1 vector encoding PPGK-controlled 1x (AEAAAK)-linker (PPGK-AEAAAK-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PPGK-driven EAAAK-linker

Expression

Mammalian

Promoter

PPGK

Available Since

June 14, 2022

Availability

Academic Institutions and Nonprofits only

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pTS2412-Tier1-PPGK-2xGS

Plasmid

#169481

Purpose

Tier-1 vector encoding PPGK-controlled 2x (GGGGS)-linker (PPGK-2xGS-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PPGK-driven GS-linker

Expression

Mammalian

Promoter

PPGK

Available Since

June 14, 2022

Availability

Academic Institutions and Nonprofits only

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pTS2378-Tier1-PhCMV-KDEL

Plasmid

#169479

Purpose

Tier-1 vector encoding PhCMV-driven ER-retention signal KDEL (PhCMV-KDEL-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven ER retention signal

Expression

Mammalian

Promoter

PhCMV

Available Since

June 14, 2022

Availability

Academic Institutions and Nonprofits only

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pVH8-Tier1-PhCMV-NLS

Plasmid

#169477

Purpose

Tier-1 vector encoding PhCMV-driven nuclear localization signal expression (PhCMV-NLS-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven nuclear localization signal

Expression

Mammalian

Promoter

PhCMV

Available Since

June 14, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmGIGYF_340-368-GST-V5His6_AH

Plasmid

#148935

Purpose

Insect Expression of DmGIGYF_340-368

Depositor

Elisa Izaurralde

Article

Ruscica et al Nucleic Acids Res. 2019 Jul 26;47(13):70

Insert

DmGIGYF_340-368 (Gyf Fly)

Expression

Insect

Available Since

May 31, 2022

Availability

Academic Institutions and Nonprofits only

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pMSCV-IRES-GFP-dog-Caspase-1/4b

Plasmid

#183365

Purpose

Stable expression of mammalian inflammatory caspase gene in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

Insert

Caspase-1/4b (CASP4 Canis lupus familiaris)

Use

Retroviral

Tags

Myc

Promoter

MESV

Available Since

May 5, 2022

Availability

Academic Institutions and Nonprofits only

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pMSCV-IRES-GFP-dog-Caspase-1/4a

Plasmid

#183363

Purpose

Stable expression of mammalian inflammatory caspase gene in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

Insert

Caspase-1/4a (CASP4 Canis lupus familiaris)

Use

Retroviral

Tags

Myc

Promoter

MESV

Available Since

May 5, 2022

Availability

Academic Institutions and Nonprofits only

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pETM-60-NusA-DmGe1_1220-1354_G

Plasmid

#146420

Purpose

Bacterial Expression of DmGe1_1220-1354

Depositor

Elisa Izaurralde

Article

Jinek et al RNA. 2008 Oct . 14(10):1991-8.

Insert

DmGe1_1220-1354 (Ge-1 Fly)

Expression

Bacterial

Available Since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pLBC0140

Plasmid

#172794

Purpose

Level 0 module containing CDS2ns_mCHERRY

Depositor

Benjamin Field

Article

Velay et al Plant Methods. 2022 May 26;18(1):69. doi

Insert

CDS mCHERRY

Use

Synthetic Biology

Mutation

no

Available Since

April 29, 2022

Availability

Academic Institutions and Nonprofits only

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pLBC0090

Plasmid

#172788

Purpose

Level 0 module containing NT2_Start

Depositor

Benjamin Field

Article

Velay et al Plant Methods. 2022 May 26;18(1):69. doi

Insert

Spacer module with start codon.

Use

Synthetic Biology

Mutation

no

Available Since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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pLBC0110

Plasmid

#172790

Purpose

Level 0 module containing CDS1_spacer

Depositor

Benjamin Field

Article

Velay et al Plant Methods. 2022 May 26;18(1):69. doi

Insert

Empty spacer module

Use

Synthetic Biology

Mutation

no

Available Since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MS2-HA-DmRoquin_AD

Plasmid

#148531

Purpose

Mammalian Expression of DmRoquin

Depositor

Elisa Izaurralde

Article

Sgromo et al Nat Commun. 2017 Feb 6;8:14307. doi: 10.

Insert

DmRoquin (roq Fly)

Expression

Mammalian

Available Since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1C-Fluc-CG10011_C

Plasmid

#146027

Purpose

Insect Expression of CG10011-3UTR

Depositor

Elisa Izaurralde

Article

Eulalio et al Genes Dev. 2007 Oct 15. 21(20):2558-70.

Insert

CG10011-3UTR (CG10011 Fly)

Expression

Insect

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmLSm2_A

Plasmid

#145887

Purpose

Insect Expression of DmLSm2

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell Biol. 2007 Dec . 27(24):8600-11

Insert

DmLSm2 (CG10418 Fly)

Expression

Insect

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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