We narrowed to 20,560 results for: otos

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mEGFP-HRas S17N

Plasmid

#18665

Depositor

Karel Svoboda

Article

Yasuda et al Nat Neurosci. 2006 Feb . 9(2):283-91.

Insert

H-Ras (HRAS Human)

Tags

monomeric EGFP

Expression

Mammalian

Mutation

S17N mutation in H-Ras

Available Since

July 2, 2008

Availability

Academic Institutions and Nonprofits only

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mEGFP-HRas S17N

Plasmid

#18665

Depositor

Karel Svoboda

Article

Yasuda et al Nat Neurosci. 2006 Feb . 9(2):283-91.

Insert

H-Ras (HRAS Human)

Tags

monomeric EGFP

Expression

Mammalian

Mutation

S17N mutation in H-Ras

Available Since

July 2, 2008

Availability

Academic Institutions and Nonprofits only

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CRY2(535, L348F)-mCherry

Plasmid

#75372

Purpose

Expresses fusion of Arabidopsis CRY2 (residues 1-535, L348F)-mCherry

Depositor

Chandra Tucker

Article

Taslimi et al Nat Chem Biol. 2016 Apr 11. doi: 10.1038

Insert

CRY2 residues 1-535 (CRY2 Mustard Weed)

Tags

mCherry

Expression

Mammalian

Mutation

L348F

Promoter

CMV

Available Since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

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CRY2(535, L348F)-mCherry

Plasmid

#75372

Purpose

Expresses fusion of Arabidopsis CRY2 (residues 1-535, L348F)-mCherry

Depositor

Chandra Tucker

Article

Taslimi et al Nat Chem Biol. 2016 Apr 11. doi: 10.1038

Insert

CRY2 residues 1-535 (CRY2 Mustard Weed)

Tags

mCherry

Expression

Mammalian

Mutation

L348F

Promoter

CMV

Available Since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pCMV-Target-AIDmax

Plasmid

#139107

Purpose

Mammalian Target-AIDmax expression vector (pSI-626)

Depositor

Nozomu Yachie

Article

Sakata et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

Insert

Bi_partite_NLS-SpCas9(D10A)-SH3_linker-PmCDA1-UGI-SV40NLS

Use

CRISPR

Expression

Mammalian

Promoter

CMV promoter

Available Since

June 1, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-Target-AIDmax

Plasmid

#139107

Purpose

Mammalian Target-AIDmax expression vector (pSI-626)

Depositor

Nozomu Yachie

Article

Sakata et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

Insert

Bi_partite_NLS-SpCas9(D10A)-SH3_linker-PmCDA1-UGI-SV40NLS

Use

CRISPR

Expression

Mammalian

Promoter

CMV promoter

Available Since

June 1, 2020

Availability

Academic Institutions and Nonprofits only

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CRY2PHR(W349R)-mCherry

Plasmid

#75370

Purpose

Expresses fusion of Arabidopsis CRY2 (residues 1-498, W349R fast-cycling mutant) fused to mCherry

Depositor

Chandra Tucker

Article

Taslimi et al Nat Chem Biol. 2016 Apr 11. doi: 10.1038

Insert

CRY2 residues 1-498 (CRY2 Mustard Weed)

Tags

mCherry

Expression

Mammalian

Mutation

W349R

Promoter

CMV

Available Since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

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CRY2PHR(W349R)-mCherry

Plasmid

#75370

Purpose

Expresses fusion of Arabidopsis CRY2 (residues 1-498, W349R fast-cycling mutant) fused to mCherry

Depositor

Chandra Tucker

Article

Taslimi et al Nat Chem Biol. 2016 Apr 11. doi: 10.1038

Insert

CRY2 residues 1-498 (CRY2 Mustard Weed)

Tags

mCherry

Expression

Mammalian

Mutation

W349R

Promoter

CMV

Available Since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pECE-HA-AMPKbeta1 wt

Plasmid

#31666

Depositor

Anne Brunet

Article

Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub

Insert

protein kinase, AMP-activated, beta 1 non-catalytic subunit (PRKAB1 Human)

Tags

HA (YDVPDYASL)

Expression

Mammalian

Available Since

April 11, 2012

Availability

Academic Institutions and Nonprofits only

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pECE-HA-AMPKbeta1 wt

Plasmid

#31666

Depositor

Anne Brunet

Article

Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub

Insert

protein kinase, AMP-activated, beta 1 non-catalytic subunit (PRKAB1 Human)

Tags

HA (YDVPDYASL)

Expression

Mammalian

Available Since

April 11, 2012

Availability

Academic Institutions and Nonprofits only

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pHR-CMV-TetO2_3C-Twin-Strep_IRES-mTurquoise2

Plasmid

#113886

Purpose

Mammalian (inducible) protein expression, bicistronic expression of cytosolic mTurquoise2

Depositor

A. Radu Aricescu

Article

Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

Type

Empty backbone

Use

Lentiviral

Tags

3C-Twin-Strep

Promoter

CMV-MIE-TetO2

Available Since

Aug. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pHR-CMV-TetO2_3C-Twin-Strep_IRES-mTurquoise2

Plasmid

#113886

Purpose

Mammalian (inducible) protein expression, bicistronic expression of cytosolic mTurquoise2

Depositor

A. Radu Aricescu

Article

Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

Type

Empty backbone

Use

Lentiviral

Tags

3C-Twin-Strep

Promoter

CMV-MIE-TetO2

Available Since

Aug. 24, 2018

Availability

Academic Institutions and Nonprofits only

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tdTomato-Gamma-Tubulin-17

Plasmid

#58099

Purpose

Localization: Centrosomes, Excitation: 554, Emission: 581

Depositor

Michael Davidson

Article

Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

Insert

Gamma-Tubulin (TUBG1 Human)

Tags

tdTomato

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 19, 2014

Availability

Academic Institutions and Nonprofits only
tdTomato-Gamma-Tubulin-17

Plasmid

#58099

Purpose

Localization: Centrosomes, Excitation: 554, Emission: 581

Depositor

Michael Davidson

Article

Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

Insert

Gamma-Tubulin (TUBG1 Human)

Tags

tdTomato

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 19, 2014

Availability

Academic Institutions and Nonprofits only
pCXLE-Fbx15-cont2

Plasmid

#27081

Depositor

Shinya Yamanaka

Article

Okita et al Nat Methods. 2011 May;8(5):409-12

Insert

FBXO15 (FBXO15 Human)

Expression

Mammalian

Available Since

Sept. 12, 2011

Availability

Academic Institutions and Nonprofits only

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pCXLE-Fbx15-cont2

Plasmid

#27081

Depositor

Shinya Yamanaka

Article

Okita et al Nat Methods. 2011 May;8(5):409-12

Insert

FBXO15 (FBXO15 Human)

Expression

Mammalian

Available Since

Sept. 12, 2011

Availability

Academic Institutions and Nonprofits only

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pFP5370 ss-FIREmate-HA- KDEL-IVS-IRES- TNF-SBP- mCherry-FIREtag

Plasmid

#216696

Purpose

Synchronize trafficking of TNFalpha from the ER (RUCH system)

Depositor

Franck Perez

Article

Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s

Inserts

FIREmate-KDEL
Tumor Necrosis Factor alpha (TNFa)

Tags

FIREtag, Streptavidin Binding Protein (SBP), and …

Expression

Mammalian

Promoter

CMV

Available Since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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pFP5370 ss-FIREmate-HA- KDEL-IVS-IRES- TNF-SBP- mCherry-FIREtag

Plasmid

#216696

Purpose

Synchronize trafficking of TNFalpha from the ER (RUCH system)

Depositor

Franck Perez

Article

Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s

Inserts

FIREmate-KDEL
Tumor Necrosis Factor alpha (TNFa)

Tags

FIREtag, Streptavidin Binding Protein (SBP), and …

Expression

Mammalian

Promoter

CMV

Available Since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pMK312 (TOP2A CRISPR)

Plasmid

#140654

Purpose

TOP2A tagging CRISPR

Depositor

Masato Kanemaki

Article

Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

Insert

TOP2A (TOP2A Human)

Use

CRISPR

Expression

Mammalian

Available Since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pMK312 (TOP2A CRISPR)

Plasmid

#140654

Purpose

TOP2A tagging CRISPR

Depositor

Masato Kanemaki

Article

Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

Insert

TOP2A (TOP2A Human)

Use

CRISPR

Expression

Mammalian

Available Since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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plenti2-EF1a-ChR2-EYFP-WRPE

Plasmid

#125255

Purpose

3rd gen lentiviral expression of ChR2 with mutation fused to EYFP driven by EF1a promoter for optogenetic activation

Depositor

Roger D. Kamm

Article

Osaki et al Sci Adv. 2018 Oct 10;4(10):eaat5847. doi

Insert

hChR2

Use

Lentiviral

Tags

EYFP

Expression

Mammalian

Available Since

Jan. 14, 2020

Availability

Academic Institutions and Nonprofits only

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plenti2-EF1a-ChR2-EYFP-WRPE

Plasmid

#125255

Purpose

3rd gen lentiviral expression of ChR2 with mutation fused to EYFP driven by EF1a promoter for optogenetic activation

Depositor

Roger D. Kamm

Article

Osaki et al Sci Adv. 2018 Oct 10;4(10):eaat5847. doi

Insert

hChR2

Use

Lentiviral

Tags

EYFP

Expression

Mammalian

Available Since

Jan. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pGP-AAV-syn-flex-iGABASnFR2n-WPRE

Plasmid

#218878

Purpose

AAV-mediated expression of improved GABA sensor (negative change in fluorescence)

Depositor

GENIE Project

Article

Kolb et al bioRxiv 2025.03.25.644953

Insert

iGABASnFR2n

Use

AAV and Cre/Lox

Expression

Mammalian

Mutation

S99A F104H R168P

Promoter

Synapsin

Available Since

June 18, 2024

Availability

Academic Institutions and Nonprofits only

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pGP-AAV-syn-flex-iGABASnFR2n-WPRE

Plasmid

#218878

Purpose

AAV-mediated expression of improved GABA sensor (negative change in fluorescence)

Depositor

GENIE Project

Article

Kolb et al bioRxiv 2025.03.25.644953

Insert

iGABASnFR2n

Use

AAV and Cre/Lox

Expression

Mammalian

Mutation

S99A F104H R168P

Promoter

Synapsin

Available Since

June 18, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pHR-CMV-TetO2_3C-Avi-His6_IRES-mRuby2

Plasmid

#113889

Purpose

Mammalian (inducible) protein expression, in vivo biotinylation, bicistronic expression of cytosolic mRuby2

Depositor

A. Radu Aricescu

Article

Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

Type

Empty backbone

Use

Lentiviral

Tags

3C-Avi-His6

Promoter

CMV-MIE-TetO2

Available Since

Aug. 27, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pHR-CMV-TetO2_3C-Avi-His6_IRES-mRuby2

Plasmid

#113889

Purpose

Mammalian (inducible) protein expression, in vivo biotinylation, bicistronic expression of cytosolic mRuby2

Depositor

A. Radu Aricescu

Article

Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

Type

Empty backbone

Use

Lentiviral

Tags

3C-Avi-His6

Promoter

CMV-MIE-TetO2

Available Since

Aug. 27, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pSKC11 - UTRQ

Plasmid

#110490

Purpose

Monitor the expression of Luciferase driven by the NRAS 5'-UTR.

Depositor

Shankar Balasubramanian

Article

Kumari et al Nat Chem Biol. 2007 Apr;3(4):218-21. doi

Insert

NRAS 5'-UTR (bp 1-254) (NRAS Human)

Use

Luciferase

Tags

Firefly luciferase

Promoter

T7

Available Since

July 20, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pSKC11 - UTRQ

Plasmid

#110490

Purpose

Monitor the expression of Luciferase driven by the NRAS 5'-UTR.

Depositor

Shankar Balasubramanian

Article

Kumari et al Nat Chem Biol. 2007 Apr;3(4):218-21. doi

Insert

NRAS 5'-UTR (bp 1-254) (NRAS Human)

Use

Luciferase

Tags

Firefly luciferase

Promoter

T7

Available Since

July 20, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
Gal4BD-CRY2(535)

Plasmid

#75369

Purpose

Yeast two-hybrid expression vector, expresses Gal4BD fused to Arabidopsis CRY2 (residues 1-535)

Depositor

Chandra Tucker

Article

Taslimi et al Nat Chem Biol. 2016 Apr 11. doi: 10.1038

Insert

CRY2 (CRY2 Mustard Weed)

Tags

Gal4BD

Expression

Yeast

Promoter

ADH1

Available Since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

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Gal4BD-CRY2(535)

Plasmid

#75369

Purpose

Yeast two-hybrid expression vector, expresses Gal4BD fused to Arabidopsis CRY2 (residues 1-535)

Depositor

Chandra Tucker

Article

Taslimi et al Nat Chem Biol. 2016 Apr 11. doi: 10.1038

Insert

CRY2 (CRY2 Mustard Weed)

Tags

Gal4BD

Expression

Yeast

Promoter

ADH1

Available Since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge