We narrowed to 10,238 results for: ada

1. pTwist-SARS-CoV-2 Spike-deltaC18 HK.3

   Plasmid

   #212572

   Purpose

   Encodes SARS-CoV-2 spike for HK.3 pseudovirus production

   Depositor

   Alejandro Balazs

   Article

   Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

   Insert

   SARS-CoV-2 Spike HK.3 (S Severe acute respiratory syndrome coronavirus 2)
   

   Expression

   Mammalian

   Mutation

   See Depositor Comments.

   Promoter

   CAG

   Available Since

   Sept. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHAGE2-TetOminiCMV-OS

   Plasmid

   #136576

   Purpose

   Dox-inducible polycistronic lentiviral vector expressing mouse Oct4, Sox2

   Depositor

   Hans Schöler

   Article

   Velychko et al Cell Stem Cell. 2019 Oct 30. pii: S1934-

   Insert

   Oct4-P2A-Sox2 (Pou5f1, Sox2 Mouse)
   

   Use

   Lentiviral

   Expression

   Bacterial

   Promoter

   tetO-miniCMV (dox-inducible)

   Available Since

   March 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAG-mDrc7-FLAG

   Plasmid

   #176470

   Purpose

   Expression vector of mouse dynein regulatory complex subunit 7 (Drc7) tagged with FLAG at C-terminus.

   Depositor

   Masahito Ikawa

   Article

   Morohoshi et al PLoS Genet. 2020 Jan 21;16(1):e1008585.

   Insert

   dynein regulatory complex subunit 7 (Drc7 Mouse)
   

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   CAG promoter

   Available Since

   Nov. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA5FRT/TO-Ataxin3-Strep-HA

   Plasmid

   #113496

   Purpose

   Expression of human Ataxin-3 with C-terminal strep-HA tag

   Depositor

   Hemmo Meyer

   Article

   Hulsmann et al Mol Cell Proteomics. 2018 Mar 29. pii: R

   Insert

   Ataxin-3 (ATXN3 Human)
   

   Tags

   2xstrep-HA

   Expression

   Mammalian

   Mutation

   wildtype without stop codon

   Promoter

   CMV

   Available Since

   Aug. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDB-hCRY1-mClover3-CD4-bla

   Plasmid

   #179441

   Purpose

   Donor vector to knock in mClover3 C-terminal to human CRY1 gene

   Depositor

   Achim Kramer

   Article

   Gabriel et al Nat Commun. 2021 Jun 18;12(1):3796. doi:

   Insert

   mClover3
   

   Use

   CRISPR

   Tags

   His/Flag

   Promoter

   None

   Available Since

   March 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLenti CMV-MRLC1-mRuby2-IRES-PuroR

   Plasmid

   #103031

   Purpose

   Lentiviral expression of mRuby2-tagged MRLC with puromycin selection

   Depositor

   Ghassan Mouneimne

   Article

   Padilla-Rodriguez et al Nat Commun. 2018 Jul 30;9(1):2980. doi:

   Insert

   MRLC1 (MYL9 Human)
   

   Use

   Lentiviral

   Tags

   mRuby2

   Promoter

   CMV immediate early

   Available Since

   May 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. qTAG-N-Blast-ultraID-LMNB1

   Plasmid

   #207775

   Purpose

   Donor template for Blast-2A-ultraID insertion into the N-terminus of the LMNB1 locus. To be co-transfected with sgRNA plasmid px330-PITCh-LMNB1 Addgene #207770

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   LMNB1 Homology Arms flanking a Blast-ultraID Cassette (LMNB1 Human)
   

   Use

   CRISPR; Donor template

   Expression

   Mammalian

   Available Since

   Nov. 29, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pAAV-EFS-DIO-GRAB_CRFmut

   Plasmid

   #208663

   Purpose

   Expresses the genetically-encoded fluorescent corticotropin-releasing factor (CRF) control sensor GRAB_CRFmut in a cre-dependent manner

   Depositor

   Yulong Li

   Article

   Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

   Insert

   GPCR activation based corticotropin-releasing factor (CRF) contorl sensor GRAB_CRFmut
   

   Use

   AAV

   Promoter

   EFS

   Available Since

   Oct. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3-Flag-RNF168 delta RING

   Plasmid

   #133982

   Purpose

   mammalian expression vector of Flag tagged RNF168 where the N-terminus of RNF168 has been deleted (including RING domain)

   Depositor

   Daniel Durocher

   Article

   Stewart et al Cell. 2009 Feb 6;136(3):420-34. doi: 10.

   Insert

   RNF168 (RNF168 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   deletion of RING

   Promoter

   CMV

   Available Since

   Nov. 4, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pCAGGS-mNG-eDHFR(69K6)-G-alpha-q(L254A)

    Plasmid

    #178851

    Purpose

    Mammalian expression of Gαq(L254A) fused to mNeonGreen-eDHFR(69K6)

    Depositor

    Shinya Tsukiji

    Article

    Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

    Insert

    mNeonGreen-eDHFR(69K6)-Gαq(L254A) (GNAQ Human)
    

    Expression

    Mammalian

    Mutation

    Gαq: C9S/C10S/Q209L/L254A

    Promoter

    CAG

    Available Since

    July 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pKK-TEV-mCherry

    Plasmid

    #105796

    Purpose

    Expression of your protein of interest in fusion with red fluorescent protein at the C-terminus (cleavable by TEV), (PMID: 15558047). mCherry descends from the first truly monomeric red FP mRFP1.

    Depositor

    Andrzej Dziembowski

    Article

    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

    Type

    Empty backbone

    Use

    Flp-in competent

    Tags

    TEV-mCherry

    Expression

    Mammalian

    Available Since

    Feb. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGL3-noSV40-humanEC1.45

    Plasmid

    #173946

    Purpose

    Firefly luciferase enhancer reporter plasmid.

    Depositor

    Joanna Wysocka

    Article

    Long et al Cell Stem Cell. 2020 Nov 5;27(5):765-783

    Insert

    SOX9 enhancer cluster EC1.45
    

    Use

    Luciferase

    Mutation

    None

    Promoter

    SV40 promoter

    Available Since

    Sept. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAGGS-mNG-eDHFR(69K6)-G-alpha-q

    Plasmid

    #172106

    Purpose

    Mammalian expression of Gαq fused to mNeonGreen-eDHFR(69K6)

    Depositor

    Shinya Tsukiji

    Article

    Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

    Insert

    mNeonGreen-eDHFR(69K6)-Gαq (GNAQ Human)
    

    Expression

    Mammalian

    Mutation

    Gαq: C9S/C10S/Q209L

    Promoter

    CAG

    Available Since

    July 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. U6_sgRNA_CAG_ABE7.10_St1Cas9_LMD9

    Plasmid

    #136660

    Purpose

    Expresses ABE St1Cas9 LMD9 in mammalian cells along with its U6-driven sgRNA

    Depositor

    Yannick Doyon

    Article

    Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:

    Inserts

    St1Cas9 LMD-9
    sgRNA for St1Cas9
    ABE7.10
    

    Use

    CRISPR and Synthetic Biology

    Tags

    SV40 NLS and hSt1Cas9

    Expression

    Mammalian

    Promoter

    CAG and U6

    Available Since

    Feb. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. p-mCherry-C1-Aurora_CA

    Plasmid

    #98170

    Purpose

    slow cycling (open for minutes) step-function artificial anion conducting channelrhodopsin (aACR). High light sensitivity. Activation with green light, inactivation max with 625 nm (accelarates closure to ms). Not recommended for in vivo use. Codon optimized for mammalian expression.

    Depositor

    Peter Hegemann

    Article

    Wietek et al Sci Rep. 2017 Nov 2;7(1):14957. doi: 10.

    Insert

    Synthetic construct Aurora_C128A gene
    

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    V59S, E83N, E90Q, E101S, V117R, E123S, C128A, P24…

    Promoter

    CMV (+enhancer)

    Available Since

    July 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLX311 HRAS

    Plasmid

    #117749

    Purpose

    Open reading frame vector encoding HRAS

    Depositor

    William Hahn

    Article

    Hong et al Mol Cancer Res. 2019 Feb 18. pii: 1541-7

    Insert

    RASH1 (HRAS Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Closed vector so will not read through the V5 reg…

    Promoter

    EF1a

    Available Since

    Feb. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. Mouse Alpha2 delta-3 HA pMT2

    Plasmid

    #58728

    Purpose

    expression of HA tagged mouse Alpha2 delta-3 in mamalian cells

    Depositor

    Annette Dolphin

    Article

    Davies et al Proc Natl Acad Sci U S A. 2010 Jan 26;10

    Insert

    alpha2 delta-3 (Cacna2d3 Mouse)
    

    Expression

    Mammalian

    Promoter

    SV40

    Available Since

    Sept. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAG-mCherry-FKBP-GEF(ITSN1)

    Plasmid

    #222631

    Purpose

    Cdc42 activation using rapamycin

    Depositor

    Arnold Hayer

    Article

    Marshall-Burghardt et al Sci Adv. 2024 Sep 6;10(36):eadn6858. doi

    Insert

    mCherry-FKBP-GEF(ITSN1) (ITSN1 Human)
    

    Tags

    mCherry-FKBP

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    July 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLentiCriprV2-sgRNA-PER1-#1

    Plasmid

    #189987

    Purpose

    Lentiviral Cas9/sgRNA vector targeting C-terminus of hPER1, works with Addgene 189979-189982

    Depositor

    Achim Kramer

    Article

    Gabriel et al Proc Natl Acad Sci U S A. 2024 Aug 20;12

    Insert

    Period1 (PER1 Human)
    

    Use

    CRISPR and Lentiviral

    Available Since

    Feb. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDN-T2dMFot

    Plasmid

    #44558

    Depositor

    Gabor Balazsi

    Article

    Nevozhay et al PLoS Comput Biol. 2012;8(4):e1002480. do

    Inserts

    PTETREG promoter
    rtetR-M2::FFF
    

    Use

    Synthetic Biology; Expression regulator/reporter

    Expression

    Yeast

    Mutation

    Compared to rtTA, rtTA-M2 has S12G, E19G, A56P, D…

    Promoter

    PTETREG

    Available Since

    April 23, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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