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Showing: 1651 - 1700 of 6776 results

  1. HD201: pMVP (L3-L2) IRES-eGFP + polyA

    Plasmid
    #121747
    Purpose
    pMVP L3-L2 entry plasmid, contains IRES2-eGFP+ polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows expression of eGFP reporter from IRES sequence.
    Depositor
    Christopher Newgard
    Article
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Insert
    IRES2-eGFP + polyA
    Use
    Synthetic Biology; Pmvp gateway entry plasmid
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    121747-plasmid-map-sequence-id-237185

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  2. KY801: pMVP (L3-L2) P2A-eGFP + polyA

    Plasmid
    #121764
    Purpose
    pMVP L3-L2 entry plasmid, contains eGFP-P2A + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows expression of C-term eGFP linked by P2A to gene of interest.
    Depositor
    Christopher Newgard
    Article
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Insert
    P2A-eGFP + polyA
    Use
    Synthetic Biology; Pmvp gateway entry plasmid
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    121764-plasmid-map-sequence-id-237247

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  7. pR6K-GFP-CASTIB

    Plasmid
    #199655
    Purpose
    R6K plasmid with a I-B CAST but no CRISPR array
    Depositor
    IIya Finkelstein
    Insert
    CAST I-B systems
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    J23119 promoter
    Available since
    May 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    199655-plasmid-map-sequence-id-412259

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  8. pR6K-GFP-CASTV

    Plasmid
    #199657
    Purpose
    R6K plasmid encoding a type V CAST but no CRISPR array
    Depositor
    IIya Finkelstein
    Insert
    CAST V systems
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    J23119 promoter
    Available since
    May 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    199657-plasmid-map-sequence-id-412267

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  10. pAAV2-CAG-3xNLS-vsfGFP-0

    Plasmid
    #191097
    Purpose
    To express a bright green nanobody FP to label eucaryotic cell nuclei. To be used in tissue clearing methods and other fluorescent microscopy methods
    Depositor
    Pantelis Tsoulfas, Murray Blackmore
    Article
    Wang et al Elife. 2022 Jul 15;11. pii: 76254. doi:
    Insert
    3xNLS-vsfGFP-0
    Use
    AAV
    Tags
    Green fluorescent protein bound to enhancer nanob…
    Expression
    Mammalian
    Mutation
    Optimized to Human codon usage
    Promoter
    CMV and CAG
    Available since
    Dec. 16, 2022
    Availability
    Academic Institutions and Nonprofits only
    191097-plasmid-map-sequence-id-380121

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  11. pAAV-hsyn-DIO-Rpl22l1-3Flag-2A-eGFP-WPRE

    Plasmid
    #214265
    Purpose
    Expression of a Flag-tagged Rpl22I1 protein in cells expressing Cre recombinase allowing for the harvesting of actively transcribed mRNAs in that cell type.
    Depositor
    D. James Surmeier
    Insert
    Flag-tagged Rpl22I1 protein expressing Cre Recombinase (Rpl22 Mouse)
    Use
    AAV
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    N/A
    Promoter
    Synapsin
    Available since
    May 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    214265-plasmid-map-sequence-id-429396

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  13. pB6-Gfap-T2A-td-sfGFP-FNF-DTA

    Plasmid
    #137044
    Purpose
    B6J Gfap-T2A-td-sfGFP allele targeting vector, low copy number
    Depositor
    Mario Capecchi
    Insert
    Gfap (Gfap Mouse)
    Use
    Mouse Targeting
    Tags
    T2A-td-sfGFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    137044-plasmid-map-sequence-id-274424

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  22. pHR_SFFV*/Puro2a-GFP

    Plasmid
    #198222
    Purpose
    Lentiviral plasmid derived from pHR_SFFV (Addgene #79121) expressing cDNA for a codon-optimized puromycin resistance gene, followed by an FMDV 2A domain and then EGFP
    Depositor
    Eric Davis
    Insert
    EGFP
    Use
    Lentiviral
    Tags
    Codon-optimized puromycin resistance gene, follow…
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    198222-plasmid-map-sequence-id-389408

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  23. meGFP-hTRAK1

    Plasmid
    #188664
    Purpose
    human TRAK1 with N-terminal meGFP tag
    Depositor
    Thomas Schwarz
    Insert
    OIP106 (TRAK1 Human)
    Use
    Tags
    meGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 16, 2022
    Availability
    Academic Institutions and Nonprofits only
    188664-plasmid-map-sequence-id-369503

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  24. mEGFP-Keratin-17

    Plasmid
    #56465
    Purpose
    Note- This plasmid contains Keratin 18. Localization: Cytokeratin, Excitation: 488, Emission: 507
    Depositor
    Michael Davidson
    Insert
    Keratin (KRT18 Human)
    Use
    Tags
    mEGFP
    Expression
    Mammalian
    Mutation
    V282L
    Promoter
    CMV
    Available since
    Dec. 23, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  34. pALS3-Ma-sfGFP-WT

    Plasmid
    #212119
    Purpose
    Fluorescence control plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses sfGFP-WT and M. alvus Pyl-tRNA(6). p15a origin. Tetracycline resistance.
    Depositor
    Ryan Mehl
    Inserts
    SUMO-sfGFP-WT
    M. alvus Pyl-tRNA(6)
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Promoter
    araC and lpp
    Available since
    Sept. 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    212119-plasmid-map-sequence-id-444185

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  35. KZ901: pMVP (L3-L2) P2A-eGFP + WPRE

    Plasmid
    #121772
    Purpose
    pMVP L3-L2 entry plasmid, contains eGFP-P2A + WPRE for 3- or 4-component MultiSite Gateway Pro assembly. Allows expression of C-term eGFP linked by P2A to gene of interest in lentivirus vectors.
    Depositor
    Christopher Newgard
    Article
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Insert
    P2A-eGFP + WPRE
    Use
    Synthetic Biology; Pmvp gateway entry plasmid
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    121772-plasmid-map-sequence-id-237267

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  36. R26-CAG-rox-FNF-bpA-3pA-rox-td-sfGFP

    Plasmid
    #180153
    Purpose
    td-sfGFP dre reporter with Rosa26 recombineering homology arms
    Depositor
    Mario Capecchi
    Insert
    td-sfGFP
    Use
    Mouse Targeting; Dre/rox
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    Available since
    Feb. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    180153-plasmid-map-sequence-id-355318

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  37. R26-CAG-loxP-FNF-bpA-3pA-loxP-td-sfGFP

    Plasmid
    #180151
    Purpose
    td-sfGFP cre reporter with Rosa26 recombineering homology arms
    Depositor
    Mario Capecchi
    Insert
    td-sfGFP
    Use
    Cre/Lox and Mouse Targeting
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    Available since
    Feb. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    180151-plasmid-map-sequence-id-355316

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  38. H11-CAG-rox-FNF-bpA-3pA-rox-td-sfGFP

    Plasmid
    #180157
    Purpose
    td-sfGFP dre reporter with Hipp11 recombineering homology arms
    Depositor
    Mario Capecchi
    Insert
    td-sfGFP
    Use
    Mouse Targeting; Dre/rox
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    Available since
    Feb. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    180157-plasmid-map-sequence-id-355323

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  39. H11-CAG-loxP-FNF-bpA-3pA-loxP-td-sfGFP

    Plasmid
    #180155
    Purpose
    td-sfGFP cre reporter with Hipp11 recombineering homology arms
    Depositor
    Mario Capecchi
    Insert
    td-sfGFP
    Use
    Cre/Lox and Mouse Targeting
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    Available since
    Feb. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    180155-plasmid-map-sequence-id-355320

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  40. pMuLE_EXPR_CMV-eGFP_TOP-iRFP_PTCH1-mTurquoise2_CBF-tdTomato

    Plasmid
    #113865
    Purpose
    Triple pathway reporter, 3P-Fluor; wnt-iRFP, hedgehog-mTurquoise2, notch-tdTomato; plus CMV-eGFP. Gateway expression vector for lentivirus generation.
    Depositor
    Manfred Ogris
    Article
    Maier et al PLoS One. 2019 Dec 20;14(12):e0226570. d
    Inserts
    CMV-eGFP
    TOP-iRFP
    PTCH1-mTurquoise2
    CBF-tdTomato
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    113865-plasmid-map-sequence-id-220242

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  41. pCMV-T7-SpCas9-P2A-EGFP (RTW3027)

    Plasmid
    #139987
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized SpCas9 with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Walton et al Science. 2020 Apr 17;368(6488):290-296.
    Insert
    human codon optimized SpCas9 with BPNLS-3xFLAG-P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV and T7
    Available since
    March 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    139987-plasmid-map-sequence-id-275335

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  45. pR6K-GFP-CASTIF

    Plasmid
    #199653
    Purpose
    R6K plasmid encoding a type I-F CAST but no CRISPR array
    Depositor
    IIya Finkelstein
    Insert
    CAST I-F systems
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    J23119 promoter
    Available since
    May 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    199653-plasmid-map-sequence-id-412253

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  48. pAAV GFP

    Plasmid
    #213685
    Purpose
    Expresses Green Fluorescent Protein (GFP) with an N-terminal twin-Strep tag
    Depositor
    Thomas Kukar
    Article
    Root et al Cell Rep. 2024 Nov 19;43(12):114985. doi
    Insert
    Twin-Strep GFP
    Use
    AAV
    Tags
    Twin-Strep
    Expression
    Mutation
    Promoter
    cytomegalovirus enhancer/chicken β-actin promoter
    Available since
    Dec. 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    213685-plasmid-map-sequence-id-455753

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  50. pHBS1187 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 N-Q]

    Plasmid
    #107847
    Purpose
    To test the effect of sequence on TDP43 splicing activity
    Depositor
    Rajat Rohatgi
    Insert
    TARDBP mutant (TARDBP Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    All asparagine to glutamine in splicing reporter
    Promoter
    Available since
    April 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    107847-plasmid-map-sequence-id-203699

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Showing: 1651 - 1700 of 6776 results