We narrowed to 73,101 results for: LAS

1. pSG81-pLas-tetO-deGFPssrA

   Plasmid

   #102454

   Purpose

   Expresses deGFP fluorescent protein with ssrA degradation tag in E. coli. The expression is controlled by a pLas-tetO combinatorial promoter.

   Depositor

   Richard Murray

   Article

   Guo et al ACS Synth Biol. 2019 Mar 15;8(3):606-610

   Insert

   deGFP
   

   Use

   Tags

   ssrA

   Expression

   Bacterial

   Mutation

   Promoter

   pLas-tetO

   Available since

   Oct. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. U6-sgfiller-eCas9-T2A-BlastR

   Plasmid

   #172437

   Purpose

   Expresses eCas9, BlastR and sgRNA cloning site

   Depositor

   Tyler Jacks

   Article

   Freed-Pastor et al Cancer Cell. 2021 Oct 11;39(10):1342-136

   Inserts

   eCas9
   BlastR
   

   Use

   CRISPR, Lentiviral, and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMCP-GPI Control Plasmid

   Plasmid

   #101132

   Purpose

   Expression of MCP-Tagged GPI anchor in Mammalian cells

   Depositor

   Ana Egana, New England Biolabs

   Insert

   GPI
   

   Use

   Tags

   MCP-tag (Mutant ACP- tag)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 27, 2017

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pf CAG mc2 IRES blast

   Plasmid

   #206235

   Purpose

   For expressing cloned exons as circular RNA.

   Depositor

   Simon Conn, Brett Stringer

   Article

   Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

   Type

   Empty backbone

   Use

   Lentiviral and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti-hU6-mU6-BlasGO

   Plasmid

   #136906

   Purpose

   Lentivirus for base editing activatable Blasticidin resistance gene expression in mammalian cells. All in one vector with sgBlasGO.

   Depositor

   Lukas Dow

   Article

   Katti et al Nucleic Acids Res. 2020 Feb 29. pii: 576

   Insert

   BlasCyGO
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   ATG>ACG

   Promoter

   SFFV

   Available since

   Feb. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLV CDHblast Rev-EGFP-V5

   Plasmid

   #175164

   Purpose

   Lentiviral expression of Rev-EGFP-V5

   Depositor

   Larry Gerace

   Article

   Hammond et al J Mol Biol. 2018 Feb 16;430(4):537-553.

   Insert

   rev
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti.PGK.blast-Flag-MFF-S155,172D

   Plasmid

   #74442

   Purpose

   Lentiviral expression vector containing flag tagged S155,172D MFF isoform 5 mutant

   Depositor

   Reuben Shaw

   Article

   Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

   Insert

   Flag-MFF (MFF Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Ser 155 Asp, Ser 172 Asp (Numbering based on MFF …

   Promoter

   Available since

   May 10, 2016

   Availability

   Academic Institutions and Nonprofits only

8. qTAG-N-Blast-mScarlet-ACTB

   Plasmid

   #207753

   Purpose

   Donor template for Blast-2A-mScarlet insertion into the N-terminus of the ACTB locus for actin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-ACTB Addgene #207748

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   ACTB Homology Arms flanking a Blast-mScarlet Cassette (ACTB Human)
   

   Use

   CRISPR; Donor template

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 1, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. PAX6 CKO 2/7 Donor plasmid

   Plasmid

   #68467

   Purpose

   donor plasmid for targeting PAX6 locus

   Depositor

   Su-Chun Zhang

   Article

   Chen et al Cell Stem Cell. 2015 Jul 1. pii: S1934-5

   Insert

   FRT flanked exon4 of PAX6 gene (PAX6 Human)
   

   Use

   Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   mouse PGK promoter

   Available since

   Nov. 25, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. qTAG-C-moxGFP-Blast-H2BC11

    Plasmid

    #207757

    Purpose

    Donor template for moxGFP-2A-Blast insertion into the C-terminus of the H2BC11 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 Addgene #207755

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    H2BC11 Homology Arms flanking a moxGFP-Blast Cassette (H2BC11 Human)
    

    Use

    CRISPR; Donor template

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 16, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. qTAG-C-mNeon-Blast-TUBB4B

    Plasmid

    #207786

    Purpose

    Donor template for mNeon-2A-Blast insertion into the C-terminus of the TUBB4B locus for tubulin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TUBB4B Addgene #207785

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    TUBB4B Homology Arms flanking a mNeon-Blast Cassette (TUBB4B Human)
    

    Use

    CRISPR; Donor template

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pACP-GPI Control Plasmid

    Plasmid

    #101131

    Purpose

    Expression of ACP-Tagged GPI anchor in Mammalian cells

    Depositor

    Ana Egana, New England Biolabs

    Insert

    GPI
    

    Use

    Tags

    ACP-Tag (ACP wt)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 27, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. lenti-Cas9-VQR-Blast

    Plasmid

    #87155

    Purpose

    lentiviral expression of SpCas9-VQR (NGA PAM restricted)

    Depositor

    Daniel Bauer

    Article

    Canver et al Nat Genet. 2017 Feb 20. doi: 10.1038/ng.

    Insert

    SpCas9-VQR(D1135V,R1335Q,T1337R)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    D1135V,R1335Q,T1337R

    Promoter

    Available since

    Feb. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. plex_307-EYFP-ccdB-blasticidin

    Plasmid

    #201131

    Purpose

    pLEX_307 plasmid with N-terminal EYFP tag and blasticidin marker

    Depositor

    Alejandro Chavez

    Type

    Empty backbone

    Use

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1α

    Available since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMXS-IRES-Blast SHMT1

    Plasmid

    #106304

    Purpose

    Express SHMT1

    Depositor

    Richard Possemato

    Article

    Minton et al Mol Cell. 2018 Feb 15;69(4):610-621.e5.

    Insert

    serine hydroxymethyltransferase 1 (SHMT1 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. JN202: pMVP/SB/Blast-DEST

    Plasmid

    #121864

    Purpose

    pMVP destination vector, empty Sleeping Beauty transposon vector backbone w/ Blast selection cassette

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Type

    Empty backbone

    Use

    Sleeping beauty transposon, pmvp gateway destinat…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. T7 promoter + rpsL reporter plasmid

    Plasmid

    #156455

    Purpose

    A mutational reporter plasmid that can gain Streptomycin and/or Tetracycline resistance upon mutation. Contains T7 promoter.

    Depositor

    Matthew D. Shoulders

    Article

    Moore et al J Am Chem Soc. 2018 Sep 19;140(37):11560

    Inserts

    rpsL
    TcR
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    ATG start codon mutated to ACG

    Promoter

    tac and tet

    Available since

    Oct. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBlastR-5xUAS-sfGFP-GMRWhite

    Plasmid

    #165907

    Purpose

    Blasticidin resistant 5xUAS sfGFP GMR-White CDS response vector. Contains an Alpha level GB2.0 entry point for addition of custom assemblies utilizing blue-white bacterial colony screening

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Insert

    Selectable UAS Response Vector
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLEX-FKBP-Kras-Blasticidin

    Plasmid

    #120714

    Purpose

    Expresses membrane-targeted recruiter protein in mammalian cells & for virus production

    Depositor

    Paul Khavari

    Article

    Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

    Insert

    KRAS4B (KRAS Human)
    

    Use

    Lentiviral

    Tags

    FKBP and mCherry

    Expression

    Mammalian

    Mutation

    amino acids 158-188

    Promoter

    CMV

    Available since

    Jan. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pACP-ADRβ2 Control Plasmid

    Plasmid

    #101128

    Purpose

    Expression of ACP-Tagged β2 Adrenergic Receptor in Mammalian cells

    Depositor

    Ana Egana, New England Biolabs

    Insert

    Beta-2-Adrenergic Receptor (ADRB2 Human)
    

    Use

    Tags

    ACP-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 27, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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