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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 3,270 results for: Streptococcus

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Showing: 661 - 680 of 3270 results

  3. Cas9 NGFR

    Plasmid
    #155279
    Purpose
    Lentiviral Cas9 expression with NGFR marker
    Depositor
    Martin Carroll
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    spCas9
    Expression
    Mammalian
    Available since
    Aug. 31, 2020
    Availability
    Academic Institutions and Nonprofits only
    155279-plasmid-map-sequence-id-309983

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  10. pG3H-PE3max-attR1R2

    Plasmid
    #213049
    Purpose
    Destination vector Expressing nCas9 and RT fusion protein and carrying Gateway recombination sites
    Depositor
    Bing Yang
    Publication
    Gupta et al Plant Commun. 2023 Oct 26:100741. doi: 1
    Inserts
    Cas9 nickase
    M-MLV Reverse Transcriptase
    Use
    Note: this plasmid needs helper pvs1-vir2 for rep…
    Expression
    Plant
    Mutation
    H840A, R240K, N413K,
    Available since
    Feb. 8, 2024
    Availability
    Academic Institutions and Nonprofits only
    213049-plasmid-map-sequence-id-422145

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  11. pHis-parallel1-NLSH2BCas9

    Plasmid
    #112065
    Purpose
    Expression and purification of NLSH2BCas9 for fungal gene editing
    Depositor
    Jeff Coleman
    Publication
    Wang et al Fungal Genet Biol. 2018 May 12. pii: S10
    Insert
    NLS of the Fusarium oxysporum histone H2B fused to SpCas9 that was codon optimized fro Aspergillus niger
    Use
    CRISPR
    Tags
    His tag
    Expression
    Bacterial
    Mutation
    Cas9 was codon optimized for Aspergillus niger
    Promoter
    promoter in vector
    Available since
    July 31, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    112065-plasmid-map-sequence-id-217190

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  12. pLenti spCas9 T2A iRFP670 P2A puro

    Plasmid
    #122182
    Purpose
    The plasmid codes for a Flag-spCas9 protein, a iRFP670 fluorescent protein and a puromycin resistance. The plasmid has two BsmBI acceptor sites to insert gRNA expressing sequences.
    Depositor
    Raphael Gaudin
    Inserts
    spCas9 (NEWENTRY )
    iRFP670
    Use
    Lentiviral
    Tags
    T2A
    Expression
    Mammalian
    Available since
    Feb. 28, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    122182-plasmid-map-sequence-id-237289

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  13. MSP2292

    Plasmid
    #70707
    Purpose
    Bacterial expression plasmid for KKH SaCas9 & sgRNA targeted to site 2: T7-humanSaCas9(E782K/N968K/R1015H)-NLS-3xFLAG-T7-Sa-sgRNA(84) #2
    Depositor
    Keith Joung
    Publication
    Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038
    Inserts
    mammalian codon-optimized KKH variant Staphylococcus aureus Cas9
    SaCas9 sgRNA (+84)
    Use
    CRISPR
    Tags
    NLS-3xFLAG
    Expression
    Bacterial
    Mutation
    E782K, N968K and R1015H in SaCas9
    Promoter
    T7
    Available since
    Dec. 23, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    70707-plasmid-map-sequence-id-127770

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  16. pX330 EGFR-sgRNA

    Plasmid
    #188633
    Purpose
    A human codon-optimized SpCas9 and chimeric guide RNA targeting C-terminus of human EGFR
    Depositor
    Jared Toettcher
    Publication
    Farahani et al Elife. 2023 May 22;12:e82863. doi: 10.75
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    humanized S. pyogenes Cas9
    Use
    CRISPR
    Tags
    3xFLAG
    Expression
    Mammalian
    Promoter
    CBh
    Available since
    Aug. 16, 2022
    Availability
    Academic Institutions and Nonprofits only
    188633-plasmid-map-sequence-id-369478

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Showing: 661 - 680 of 3270 results