Addgene

We narrowed to 1,459 results for: Streptococcus

Showing: 661 - 680 of 1459 results

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pKMD106e - intein-Cas9(S219)

Plasmid

#64190

Purpose

Expresses intein-Cas9(S219) in mammalian cells

Depositor

David Liu

Article

Davis et al Nat Chem Biol. 2015 May;11(5):316-8. doi

Insert

mammalian codon-optimized S. pyogenes intein-Cas9(S219) - NLS - 3x FLAG

Use

CRISPR

Tags

NLS - 3x FLAG (C terminal on insert)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 18, 2015

Availability

Academic Institutions and Nonprofits only

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pRH2502

Plasmid

#84379

Purpose

Expression of dcas9 D10A H840A from a TetR-regulated uvtetO promoter

Depositor

Robert Husson

Article

Singh et al Nucleic Acids Res. 2016 Jul 12. pii: gkw

Insert

dcas9

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Aspartate at position 10 changed to Alanine, Hist…

Promoter

uv15tetO

Available since

Feb. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pRS315e_pGal-nCas9(D10A)-PmCDA1

Plasmid

#79617

Purpose

Expresses nCas9(D10A)-PmCDA1 in yeast cells

Depositor

Akihiko Kondo

Article

Nishida et al Science. 2016 Aug 4. pii: aaf8729.

Insert

SpCas9

Use

Tags

PmCDA1

Expression

Yeast

Mutation

D10A for nickase Cas9

Promoter

pGal1

Available since

July 27, 2016

Availability

Academic Institutions and Nonprofits only

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pJIT163-2NLSCas9

Plasmid

#53064

Purpose

Expression of rice codon-optimized Cas9 in plant cells

Depositor

Caixia Gao

Article

Shan et al Nat Biotechnol. 2013 Aug;31(8):686-8. do

Insert

Cas9

Use

Plant expression

Tags

Expression

Mutation

Rice codon optimized

Promoter

2x35S

Available since

Aug. 21, 2014

Availability

Academic Institutions and Nonprofits only

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pRS315e_pGal-dCas9-PmCDA1

Plasmid

#79615

Purpose

Expresses dCas9-PmCDA1 in yeast cells

Depositor

Akihiko Kondo

Article

Nishida et al Science. 2016 Aug 4. pii: aaf8729.

Insert

SpCas9

Use

Tags

PmCDA1

Expression

Yeast

Mutation

D10A and H840A for nuclease deficient Cas9

Promoter

pGal1

Available since

July 22, 2016

Availability

Academic Institutions and Nonprofits only

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pRB14

Plasmid

#52522

Purpose

expresses a myc-tagged version of hCas9 in Drosophila

Depositor

Klaus Foerstemann

Article

Bottcher et al Nucleic Acids Res. 2014 Apr 19.

Insert

S. pyogenes cas9 with humanized codon bias

Use

Tags

myc

Expression

Insect

Mutation

Promoter

tubulin

Available since

April 22, 2014

Availability

Academic Institutions and Nonprofits only

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pJT82_GalL_nCDA1Δ198-BE3

Plasmid

#145046

Purpose

Expresses nCDA1Δ198-BE3 in yeast cells

Depositor

Ralph Bock

Article

Tan et al Nat Commun. 2019 Jan 25;10(1):439. doi:

Insert

nCDA1Δ198-BE3

Use

CRISPR

Tags

Expression

Yeast

Mutation

pmCDA1(1-198AA); spCas9(D10A)

Promoter

GalL

Available since

July 10, 2020

Availability

Academic Institutions and Nonprofits only

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pKMD106h - intein-Cas9(C574)

Plasmid

#64191

Purpose

Expresses intein-Cas9(C574) in mammalian cells

Depositor

David Liu

Article

Davis et al Nat Chem Biol. 2015 May;11(5):316-8. doi

Insert

mammalian codon-optimized S. pyogenes intein-Cas9(C574) - NLS - 3x FLAG

Use

CRISPR

Tags

NLS - 3x FLAG (C terminal on insert)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 8, 2015

Availability

Academic Institutions and Nonprofits only

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His-TEV-GB1 Leu5Met

Plasmid

#128686

Purpose

Expresses GB1 Leu5Met mutant

Depositor

Sharon Rozovsky

Article

Chen et al J Phys Chem B. 2020 Jan 30;124(4):601-61

Insert

spg

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 1, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pCT

Plasmid

#60620

Purpose

Plasmid encoding iCas9 and tracrRNA on pRS415 backbone

Depositor

Huimin Zhao

Article

Bao et al ACS Synth Biol. 2014 Sep 19.

Inserts

iCas9
tracrRNA

Use

CRISPR

Tags

FLAG and SV40 NLS

Expression

Yeast

Mutation

changed Aspartate 147 to Tyrosine, Proline 411 to…

Promoter

RPR1p and TEF1p

Available since

Nov. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pJT43_GalL_BE-P(AP)3

Plasmid

#145036

Purpose

Expresses BE-P(AP)3 in yeast cells

Depositor

Ralph Bock

Article

Tan et al Nat Commun. 2019 Jan 25;10(1):439. doi:

Insert

BE-(AP)3

Use

CRISPR

Tags

Expression

Yeast

Mutation

spCas9(D10A)

Promoter

GalL

Available since

July 9, 2020

Availability

Academic Institutions and Nonprofits only

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pJT112_GalL_nCDA1-xBE3

Plasmid

#141318

Purpose

Expressing base editor nCDA1-xBE3 in yeast cells

Depositor

Ralph Bock

Article

Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

Insert

nCDA1-xBE3

Use

CRISPR

Tags

Expression

Yeast

Mutation

spCas9(D10A, A262T, R324L, S409I, E480K, E543D, M…

Promoter

GalL

Available since

July 9, 2020

Availability

Academic Institutions and Nonprofits only

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pJT45_GalL_nCDA1-BE3

Plasmid

#145038

Purpose

Expresses nCDA1-BE3 in yeast cells

Depositor

Ralph Bock

Article

Tan et al Nat Commun. 2019 Jan 25;10(1):439. doi:

Insert

nCDA1-BE3

Use

CRISPR

Tags

Expression

Yeast

Mutation

spCas9(D10A)

Promoter

GalL

Available since

Sept. 22, 2020

Availability

Academic Institutions and Nonprofits only

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LacD.1_WT_pET21a

Plasmid

#29789

Depositor

SeWon Suh

Article

Lee et al FEBS Lett. 2011 Jan 21. 585(2):307-12.

Insert

Tagatose 1,6-diphosphate aldolase (lacD.1 S. pyogenes (serotype M1))

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

March 27, 2012

Availability

Academic Institutions and Nonprofits only
pJT79_GalL_nCDA1Δ192-BE3

Plasmid

#145045

Purpose

Expresses nCDA1Δ192-BE3 in yeast cells

Depositor

Ralph Bock

Article

Tan et al Nat Commun. 2019 Jan 25;10(1):439. doi:

Insert

nCDA1Δ192-BE3

Use

CRISPR

Tags

Expression

Yeast

Mutation

pmCDA1(1-192AA); spCas9(D10A)

Promoter

GalL

Available since

July 10, 2020

Availability

Academic Institutions and Nonprofits only

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pDGE76

Plasmid

#80578

Purpose

recipient plasmid [multiplexing, nickase]; p35S:Cas9 D10A, nptII

Depositor

Johannes Stuttmann

Article

Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

Inserts

pnos:nptII-tmos
p2x35S:Cas9 D10A-tnos
BsaI-ccdB_CmR-BsaI

Use

CRISPR

Tags

Expression

Plant

Mutation

D10A

Promoter

Available since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pDGE77

Plasmid

#80579

Purpose

recipient plasmid [multiplexing, nickase]; p35S:Cas9 D10A, BASTA

Depositor

Johannes Stuttmann

Article

Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

Inserts

pnos:PAT-tnos
p2x35S:Cas9 D10A-tnos
BsaI-ccdB_CmR-BsaI

Use

CRISPR

Tags

Expression

Plant

Mutation

D10A

Promoter

Available since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pDGE78

Plasmid

#80580

Purpose

recipient plasmid [multiplexing, nickase]; pPcUbi:Cas9 D10A, nptII

Depositor

Johannes Stuttmann

Article

Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

Inserts

pnos:nptII-tmos
pPcUbi:Cas9 D10A-tnos
BsaI-ccdB_CmR-BsaI

Use

CRISPR

Tags

Expression

Plant

Mutation

D10A

Promoter

Available since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pDGE79

Plasmid

#80581

Purpose

recipient plasmid [multiplexing, nickase]; pPcUbi:Cas9 D10A, BASTA

Depositor

Johannes Stuttmann

Article

Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

Inserts

pnos:PAT-tnos
pPcUbi:Cas9 D10A-tnos
BsaI-ccdB_CmR-BsaI

Use

CRISPR

Tags

Expression

Plant

Mutation

D10A

Promoter

Available since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

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His-TEV-GB1 Initial Gly

Plasmid

#128685

Purpose

Expresses GB1 mutant with no Methionine residues

Depositor

Sharon Rozovsky

Article

Chen et al J Phys Chem B. 2020 Jan 30;124(4):601-61

Insert

spg

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 1, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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