Addgene

We narrowed to 1,261 results for: membrane gfp

Showing: 661 - 680 of 1261 results

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GFP-TIS11B

Plasmid

#121157

Purpose

Mammalian expression of GFP tagged TIS11B

Depositor

Christine Mayr

Article

Ma et al Cell. 2018 Oct 30. pii: S0092-8674(18)31

Insert

ZFP36L1 (TIS11B) (ZFP36L1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 7, 2019

Availability

Academic Institutions and Nonprofits only

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MSCV-LMP2A-IRES-GFP

Plasmid

#220352

Purpose

Expresses EBV latent gene LMP2A in mammalian cells

Depositor

Klaus Rajewsky

Article

Zhang et al Front Immunol. 2023 Dec 19;14:1331730. d

Insert

LMP2A (LMP-2A Epstein-Barr Virus (EBV) strain B95-8)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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mCherry-7aa-ActA-pEGFP-C1

Plasmid

#177406

Purpose

To express mCherry fused to mitochondria outer membrane targeting sequence "ActA". mCherry faces the cytosol.

Depositor

David Andrews

Article

Aranovich et al Mol Cell. 2012 Mar 30;45(6):754-63. doi:

Insert

ActA tail anchor (actA Listeria monocytogenes)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 27, 2022

Availability

Academic Institutions and Nonprofits only

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MSCV-LMP1-IRES-GFP

Plasmid

#220350

Purpose

Expresses EBV latent gene LMP1 in mammalian cells

Depositor

Klaus Rajewsky

Article

Zhang et al Front Immunol. 2023 Dec 19;14:1331730. d

Insert

LMP1 (LMP-1 Epstein-Barr Virus (EBV) strain B95-8)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pMP71-tCD19-mGFP

Plasmid

#174609

Purpose

expresses membrane-associated (palmitoylated) EGFP and extracellular and transmembrane domains of murine CD19

Depositor

Stanley Riddell

Article

Veatch et al J Clin Invest. 2021 Aug 16;131(16):e1441

Insert

CD19 extracellular and transmembrane domain (Cd19 Mouse)

Use

Tags

palmitoylated EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 4, 2021

Availability

Academic Institutions and Nonprofits only

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Prig-3::CD4::spGFP1-10

Plasmid

#65829

Purpose

For membrane contact

Depositor

Cori Bargmann, Kang Shen

Article

Feinberg et al Neuron. 2008 Feb 7. 57(3):353-63.

Insert

Homo sapiens CD4 molecule (CD4), transcript variant 2, mRNA (CD4 Human)

Use

Tags

spGFP1-10

Expression

Worm

Mutation

Promoter

Prig-3

Available since

Aug. 25, 2015

Availability

Academic Institutions and Nonprofits only

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Prig-3::CD4::spGFP11

Plasmid

#65830

Purpose

For membrane contact

Depositor

Cori Bargmann, Kang Shen

Article

Feinberg et al Neuron. 2008 Feb 7. 57(3):353-63.

Insert

Homo sapiens CD4 molecule (CD4), transcript variant 2, mRNA (CD4 Human)

Use

Tags

spGFP11

Expression

Worm

Mutation

Promoter

Prig-3

Available since

Aug. 25, 2015

Availability

Academic Institutions and Nonprofits only

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pEGFP-N3-Myr-TYK2

Plasmid

#139355

Purpose

Expression of membrane-anchored human TYK2 protein fused to GFP

Depositor

Sandra Pellegrini

Article

Ragimbeau et al J Biol Chem. 2001 Aug 17;276(33):30812-8

Insert

TYK2 cDNA with aa 1-16 of human Lck fused to the N-terminus (TYK2 Human)

Use

Tags

aa 1-16 of human Lck and GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 23, 2020

Availability

Academic Institutions and Nonprofits only

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GFP- SEC61B

Plasmid

#121159

Purpose

Mammalian expression of GFP tagged SEC61B, used as a general ER marker

Depositor

Christine Mayr

Article

Ma et al Cell. 2018 Oct 30. pii: S0092-8674(18)31

Insert

SEC61B (SEC61B Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 7, 2019

Availability

Academic Institutions and Nonprofits only

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GFP-HuR

Plasmid

#121162

Purpose

Mammalian expression of GFP tagged HuR

Depositor

Christine Mayr

Article

Ma et al Cell. 2018 Oct 30. pii: S0092-8674(18)31

Insert

HuR (ELAVL1 )

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 7, 2019

Availability

Academic Institutions and Nonprofits only

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GFP-Dcp1a

Plasmid

#153972

Purpose

Fluorescent protein fused to mRNA decapping enzyme 1a

Depositor

Gia Voeltz

Article

Lee et al Science. 2020 Jan 31;367(6477). pii: 367

Insert

mRNA decapping enzyme 1a (DCP1A Human)

Use

Tags

pAcGFP-C1

Expression

Mammalian

Mutation

Promoter

Available since

July 21, 2020

Availability

Academic Institutions and Nonprofits only

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GFP-HSPA8

Plasmid

#121161

Purpose

Mammalian expression of GFP tagged HSPA8

Depositor

Christine Mayr

Article

Ma et al Cell. 2018 Oct 30. pii: S0092-8674(18)31

Insert

HSPA8 (HSPA8 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 7, 2019

Availability

Academic Institutions and Nonprofits only

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GFP-Dcp1b

Plasmid

#153976

Purpose

Fluorescent protein fused to mRNA decapping enzyme 1b

Depositor

Gia Voeltz

Article

Lee et al Science. 2020 Jan 31;367(6477). pii: 367

Insert

mRNA recapping enzybe 1b (DCP1B Human)

Use

Tags

pAcGFP-C1

Expression

Mammalian

Mutation

Promoter

Available since

July 21, 2020

Availability

Academic Institutions and Nonprofits only

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pYea5.1_Notch1-L24_sfGFP

Plasmid

#207865

Purpose

Yeast 2µ vector for expression of Suc2SP-Notch1-L24_100-sfGFP-GAL4

Depositor

Dieter Langosch

Article

Ortner et al J Mol Biol. 2023 Aug 1;435(18):168218. d

Insert

Suc2SP-Notch1-L24_100-sfGFP-GAL4 fusion protein

Use

Tags

Strep-TagII

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pccGFPKAN

Plasmid

#73649

Purpose

Fluorescent reporter for transmembrane protein dimerization.

Depositor

Alessandro Senes

Article

Armstrong et al Biochim Biophys Acta. 2016 Jul 21. pii:

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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pccGFPGpA

Plasmid

#73651

Purpose

Fluorescent reporter for transmembrane protein dimerization positive control.

Depositor

Alessandro Senes

Article

Armstrong et al Biochim Biophys Acta. 2016 Jul 21. pii:

Insert

glycophorin A

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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pccGFPG83I

Plasmid

#73650

Purpose

Fluorescent reporter for transmembrane protein dimerization negative control.

Depositor

Alessandro Senes

Article

Armstrong et al Biochim Biophys Acta. 2016 Jul 21. pii:

Insert

glycophorin A- G83I

Use

Tags

Expression

Bacterial

Mutation

glycine 83 to isoleucine

Promoter

Available since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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pFSW Syt1-C2B V304A,I367A IRES GFP

Plasmid

#133821

Purpose

Encodes the C2B domain of synaptotagmin 1 with membrane-penetrating residue mutations V304A,I367A for viral expression

Depositor

Edwin Chapman

Article

Courtney et al Nat Commun. 2019 Sep 9;10(1):4076. doi:

Insert

Syt1 (Syt1 Rat)

Use

Lentiviral

Tags

IRES-GFP

Expression

Mutation

V304A, I367A

Promoter

hSyn (human synapsin I promoter)

Available since

Availability

Academic Institutions and Nonprofits only

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pFSW Syt1-C2A M173,F234A IRES GFP

Plasmid

#133825

Purpose

Encodes the C2A domain of synaptotagmin 1 with membrane-penetrating residue mutations M173,F234A for viral expression

Depositor

Edwin Chapman

Article

Courtney et al Nat Commun. 2019 Sep 9;10(1):4076. doi:

Insert

Syt1 (Syt1 Rat)

Use

Lentiviral

Tags

IRES-GFP

Expression

Mutation

M173, F234A

Promoter

hSyn (human synapsin I promoter)

Available since

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1a-LAMP-1-mGFP-IRES-Puromycin

Plasmid

#134868

Purpose

Expresses EGFP-tagged lysosomal marker

Depositor

David Andrews

Article

Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

Lysosomal-associated membrane protein 1 (LAMP1 Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

March 3, 2020

Availability

Academic Institutions and Nonprofits only

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