We narrowed to 1,214 results for: poli

1. pPB_TRE3G_scFV-sfGFP

   Plasmid

   #235573

   Purpose

   Dox-inducible expression of control superfolder (sf)GFP fused with scFV

   Depositor

   Jamie Hackett

   Article

   Policarpi et al Nat Genet. 2024 Jun;56(6):1168-1180. doi

   Insert

   superfolder (sf)fGFP
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 29, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pPB_TRE3G_dCas9-5xGCN4_Hygro

   Plasmid

   #235572

   Purpose

   Dox-inducible expression of dCas9-GCN4 & genomic integration

   Depositor

   Jamie Hackett

   Article

   Policarpi et al Nat Genet. 2024 Jun;56(6):1168-1180. doi

   Insert

   dCas9-5xGCN4
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   D10A; H840A

   Promoter

   Available since

   April 29, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHnCBS1D

   Plasmid

   #52065

   Purpose

   expresses nine genes from the H. neapolitanus carboxysome operon and CSOS1D; IPTG-inducible

   Depositor

   David Savage

   Article

   Bonacci et al Proc Natl Acad Sci U S A. 2012 Jan 10;10

   Insert

   CbbL, CbbS, CSOS2, CSOS3, CSOS4A, CSOS4B, CSOS1C, CSOS1A, CSOS1B, CSOS1D
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pNOC-stacked-GFP-aequorin

   Plasmid

   #101011

   Purpose

   Construct for expression of a calcium sensitive luciferase reporter, GFP-aequorin, and hygromycin resistance gene by a bidirectional promoter

   Depositor

   Eva Farre

   Article

   Poliner et al Plant J. 2019 Jul;99(1):112-127. doi: 10

   Type

   Empty backbone

   Use

   Algae, nannochloropsis

   Tags

   Expression

   Mutation

   Promoter

   Ribi

   Available since

   March 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pNOC-ARS-stacked-NeoR-mNeonGreen

   Plasmid

   #101249

   Purpose

   Construct for fusion expression of mNeonGreen reporter and G418 resistance gene by a bidirectional promoter

   Depositor

   Eva Farre

   Article

   Poliner et al Plant J. 2019 Jul;99(1):112-127. doi: 10

   Type

   Empty backbone

   Use

   Algae, nannochloropsis

   Tags

   Expression

   Mutation

   Promoter

   Ribi

   Available since

   May 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pNOC-stacked-aequorin

   Plasmid

   #101010

   Purpose

   Construct for expression of aequorin, calcium sensitive luciferase reporter and hygromycin resistance gene by a bidirectional promoter

   Depositor

   Eva Farre

   Article

   Poliner et al Plant J. 2019 Jul;99(1):112-127. doi: 10

   Type

   Empty backbone

   Use

   Algae, nannochloropsis

   Tags

   Expression

   Mutation

   Promoter

   Ribi

   Available since

   March 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pet11a-ABLE

   Plasmid

   #158627

   Purpose

   Amp resistant IPTG inducible 6xHis-TEV-ABLE protein for E. coli expression

   Depositor

   William DeGrado

   Article

   Polizzi et al Science. 2020 Sep 4;369(6508):1227-1233.

   Insert

   Apixaban-Binding Helical Bundle
   

   Use

   Tags

   6x His-tag and TEV cleavage tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBz118_His_CsoS2

   Plasmid

   #140841

   Purpose

   Halothiobacillus neapolitanus CsoS2 with N-terminal His-tag, pET-14b backbone

   Depositor

   David Savage

   Article

   Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

   Insert

   CsoS2
   

   Use

   Tags

   Hexahistidine tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   July 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pNOC-stacked-GeNL-Ca

   Plasmid

   #101003

   Purpose

   Construct for fusion expression of GeNL calcium reporter and neomycin/G418 resistance gene by a bidirectional promoter

   Depositor

   Eva Farre

   Article

   Poliner et al Plant J. 2019 Jul;99(1):112-127. doi: 10

   Type

   Empty backbone

   Use

   Algae, nannochloropsis expression

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 31, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pPB_mU6_enh-gRNA_Puro-T2A-BFP

    Plasmid

    #235571

    Purpose

    Enhanced guide RNA expression & genomic integration. Target gRNA sequences are cloned in via the BstXI and BlpI sites.

    Depositor

    Jamie Hackett

    Article

    Policarpi et al Nat Genet. 2024 Jun;56(6):1168-1180. doi

    Insert

    sgRNA backbone
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 29, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLz37_CB_operon_CsoS2_N1-N4

    Plasmid

    #140856

    Purpose

    Halothiobacillus neapolitanus carboxysome operon with full length CsoS2. For heterologous expression of carboxysomes in E. coli.

    Depositor

    David Savage

    Article

    Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

    Insert

    CbbL, CbbS, CsoS2 (full-length), CsoSCA, CsoS4A, CsoS4B, CsoS1C, CsoS1A, CsoS1B, CsoS1D
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBz15_Strep_Rubisco

    Plasmid

    #140839

    Purpose

    wild-type Halothiobacillus neapolitanus Form I Rubisco with StrepII tag (CbbL-StrepII, CbbS), pET-14b backbone

    Depositor

    David Savage

    Article

    Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

    Insert

    Form IA Rubisco; CbbL, CbbS
    

    Use

    Tags

    Strep II

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pNOC-CRISPR-HA

    Plasmid

    #99370

    Purpose

    Expresses Cas9-HA and sgRNA. Hygromycin resistance.

    Depositor

    Eva Farre

    Article

    Poliner et al ACS Synth Biol. 2018 Apr 20;7(4):962-968

    Type

    Empty backbone

    Use

    Algae, nannochloropsis expression

    Tags

    Cas9-HA

    Expression

    Mutation

    Promoter

    Ribi promoter

    Available since

    Feb. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pET28a-λN-βLac

    Plasmid

    #162039

    Purpose

    Expresion of the fusion protein of the λN domain of the λ phage and ampR gene (encoding the enzyme β-lactamase without the native signal sequence)

    Depositor

    Karen Polizzi

    Article

    Moya Ramirez et al Biosens Bioelectron. 2019 Jul 15;137:199

    Insert

    λN domain of the λ phage and a β-lactamase fusion protein
    

    Use

    Tags

    6xHis tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pPB_TRE3G_scFV-sfGFP-Ring1bCD

    Plasmid

    #235574

    Purpose

    Dox-inducible expression of the catalytic-domain (CD) of epigenetic effector Ring1b fused with scFV to programme H2AK119ub.

    Depositor

    Jamie Hackett

    Article

    Policarpi et al Nat Genet. 2024 Jun;56(6):1168-1180. doi

    Insert

    Ring1b (Rnf2 Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 29, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pPB_TRE3G_scFV-sfGFP-Prdm9CD

    Plasmid

    #235577

    Purpose

    Dox-inducible expression of the catalytic-domain (CD) of epigenetic effector Prdm9 fused with scFV to programme H3K4me3

    Depositor

    Jamie Hackett

    Article

    Policarpi et al Nat Genet. 2024 Jun;56(6):1168-1180. doi

    Insert

    Prdm9 (Prdm9 Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 29, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pPB_TRE3G_scFV-sfGFP-Ring1bCD-CatMut

    Plasmid

    #235583

    Purpose

    Dox-inducible expression of control catalytic-mutant Ring1b CD fused with scFV

    Depositor

    Jamie Hackett

    Article

    Policarpi et al Nat Genet. 2024 Jun;56(6):1168-1180. doi

    Insert

    Ring1b (Rnf2 Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    I53S

    Promoter

    Available since

    April 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pPB_TRE3G_scFV-sfGFP-Prdm9CD-CatMut

    Plasmid

    #235586

    Purpose

    Dox-inducible expression of control catalytic-mutant Prdm9 CD fused with scFV

    Depositor

    Jamie Hackett

    Article

    Policarpi et al Nat Genet. 2024 Jun;56(6):1168-1180. doi

    Insert

    Prdm9 (Prdm9 Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    G282A

    Promoter

    Available since

    April 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pPB_TRE3G_scFV-sfGFP-Setd2CD

    Plasmid

    #235579

    Purpose

    Dox-inducible expression of the catalytic-domain (CD) of epigenetic effector Setd2 fused with scFV to programme H3K36me3

    Depositor

    Jamie Hackett

    Article

    Policarpi et al Nat Genet. 2024 Jun;56(6):1168-1180. doi

    Insert

    Setd2 (Setd2 Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 29, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pNOC-CRISPR-GFP

    Plasmid

    #100009

    Purpose

    Expresses Cas9-GFP and sgRNA in Nannochloropsis oceanica, contains hygromycin resistance cassette, for integration into geneome

    Depositor

    Eva Farre

    Article

    Poliner et al ACS Synth Biol. 2018 Apr 20;7(4):962-968

    Type

    Empty backbone

    Use

    Algae, nannochloropsis expression

    Tags

    Cas9-GFP

    Expression

    Mutation

    Promoter

    Ribi promoter

    Available since

    Feb. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge