We narrowed to 15,942 results for: genome
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Plasmid#100935PurposeGFP, puromycin and gRNA scaffold driven by EF1a promoterDepositorInsertgRNA scaffold
UseGrna scaffoldExpressionMammalianAvailable SinceJune 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDL14
Plasmid#106901PurposepDL14 is conditionally replicating plasmid and expresses gam, bet and mutSK622A genes under control of PrhaB promoter for oligo-mediated E. coli chromosome engineering in MMR independent mannerDepositorInsertsgam
bet
mutS
lacI
rhaR
rhaS
ExpressionBacterialMutationLysine 622 was replaced by AlanineAvailable SinceJune 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
Donor-PIGA
Plasmid#153530PurposeUsed as a donor vector for the targeted correction of a mutation in PIGA exon 6DepositorInsertPIGA (a 1,991-bp fragment from intron 5 and exon 6)
UseDonor plasmidTagsN/AMutationNo mutationPromoterNo promoterAvailable SinceDec. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
PX458_AHR_1
Plasmid#101076PurposeEncodes gRNA for 3' target of human AHRDepositorInsertgRNA against AHR (AHR Human)
UseCRISPRAvailable SinceOct. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
TRE3G-CTCF-WT-HA-MND-zeo
Plasmid#232801PurposeInducible over expression of wild type version of CTCF.DepositorAvailable SinceFeb. 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pQTEV-CDO1
Plasmid#31292DepositorAvailable SinceNov. 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
pNAD1
Plasmid#154347PurposepUC19 backbone with editing template for homologous recombination mediated deletion of Nitrate reductase gene of N. oceanica and selection based on zeocin resistanceDepositorInsertShBle
UseMicroalgaeExpressionBacterialMutationnonePromoterViolaxanthin Chlorophyll a Binding Protein Precur…Available SinceNov. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
MiniCoopR U6:gRNA cdkn2a, mitfa:Cas9
Plasmid#118842PurposeTargets cdkn2a and expresses zebrafish mitfa specifically in zebrafish melanocytesDepositorInsertcdkn2a gRNA (LOC100329528 Zebrafish)
UseCRISPR; Tol2Available SinceNov. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKMD111e - intein-Cas9(S219-G521R)
Plasmid#64192PurposeExpresses intein-Cas9(S219-G521R) in mammalian cellsDepositorInsertmammalian codon-optimized S. pyogenes intein-Cas9(S219-G521R) - NLS - 3x FLAG
UseCRISPRTagsNLS - 3x FLAG (C terminal on insert)ExpressionMammalianPromoterCMVAvailable SinceJuly 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCAG MCP-VP64
Plasmid#92357PurposeCAG-driven ubiquitous expression of MCP-VP64. MCP is bacteriophage MS2 coat protein, VP64 is transcriptional activation domain. Part of SAM-mediated gene activation system in chicken embryos.DepositorInsertMCP-VP64
UseCRISPRExpressionMammalianPromoterCAGAvailable SinceAug. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAS_4xEcoLeuT(CUA)_EF1_sfGFP150TAG
Plasmid#174892Purposeamber suppression reporter sfGFP150TAG with EcoLeuT(CUA) amber suppressor tRNA cassetteDepositorInsertsfGFP
ExpressionMammalianMutation150TAG in sfGFPPromoterEF1Available SinceNov. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
PX458_KDM1A_1
Plasmid#86297PurposeEncodes gRNA for 3' target of human KDM1ADepositorInsertgRNA against KDM1A (KDM1A Human)
UseCRISPRAvailable SinceFeb. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUb-tdTomato-LP-Neo-LP
Plasmid#231509PurposeFluorescent reporter for tdTomato expression in Acanthamoeba via the Cre/loxP systemDepositorInserttdTomato fluorescent protein
UseUnspecified; Acanthamoeba castellanii neff strainPromoterAcanthamoeba polyubiquitinAvailable SinceMarch 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGAPDH-Cre-HYG
Plasmid#231510PurposeExpresses Cre recombinase in AcanthamoebaDepositorInsertCre recombinase
UseUnspecified; Acanthamoeba castellanii neff strainTagsEGFP and SV40 NLSPromoterAcanthamoeba GAPDHAvailable SinceMarch 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
PX458_KDM1A_2
Plasmid#86298PurposeEncodes gRNA for 3' target of human KDM1ADepositorInsertgRNA against KDM1A (KDM1A Human)
UseCRISPRAvailable SinceJan. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMpGE006
Plasmid#108722PurposeCas9 expression in M.polymorphaDepositorInsertAtco-Cas9-Pea3ter
UseCRISPRExpressionPlantPromoterMpEFproAvailable SinceJune 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
Lenti-eCas9-sgMIB1
Plasmid#140239PurposeLentiviral vector expressing high-specificity eSpCas9(1.1) and a gRNA targeting human MIB1DepositorInsertsgRNA targeting human MIB1
UseCRISPR and LentiviralExpressionMammalianPromoterU6Available SinceMay 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
p1192-scAAV-U6-sgRNA_Nme2Cas9_Rosa26-CB-PI-AcrIIC3Nme_FLAG_NLS
Plasmid#129530PurposeSelf-complementary AAV vector expressing Nme2Cas9 sgRNA targeting Rosa26 and AcrIIC3NmeDepositorInsertscodon-optimized AcrIIC3Nme
sgRNA_Rosa26
UseAAVTagsFLAG/NLSPromoterU6 and cytomegalovirus-enhancer chicken β-actin p…Available SinceOct. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
lenti-EGFR-L858R-nick sgRNA
Plasmid#214097PurposeLentiviral vector expressing nicking sgRNA to induce EGFR L858R mutation using PE3DepositorInsertEGFR L858R nicking sgRNA
UseLentiviralExpressionMammalianPromoterhU6Available SinceJune 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
lenti-EGFR-T790M-nick sgRNA
Plasmid#214098PurposeLentiviral vector expressing nicking sgRNA to induce EGFR T790M mutation using PE3DepositorInsertEGFR T790M nicking sgRNA
UseLentiviralExpressionMammalianPromoterhU6Available SinceJune 6, 2024AvailabilityAcademic Institutions and Nonprofits only