We narrowed to 11,727 results for: EMB
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Plasmid#188392PurposeCu2+-dependent expression of red fluorescent protein in yeast cellsDepositorInsertFusionRed
ExpressionYeastPromoterCUP1 promoterAvailable SinceApril 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPW3763 : CMVd3-HsIRE1dLKR-HaloTag
Plasmid#185685PurposeLow-level transient expression of HsIRE1 lacking the linker, kinase, and RNase domains, with a C-terminal HaloTag.DepositorInsertERN1 (ERN1 Human, Synthetic)
TagsHaloTagExpressionMammalianMutationDeletion of linker, kinase, and Rnase domainsAvailable SinceMarch 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
trp1D-proLYS2-GFP-PKCdelta(C1a+C1b)-NAT
Plasmid#184774PurposeMarker for DAG. Yeast expression of mouse PKCdelta under LSY2 promoter. Uses antibiotic resistance marker natMX6. Replaces endogenous TRP1 upon genome integration, leading to trp1D.DepositorAvailable SinceFeb. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-TAPBPR-TM
Plasmid#178645PurposeMammalian expression of FLAG-tagged TAPBPR with MHC TMDepositorInsertTAPBPR (TAPBPL Human)
TagsFLAG and Signal/leader sequence from HLA class I …ExpressionMammalianMutationswitched transmembrane domain with that of MHC-IPromoterCMVAvailable SinceJuly 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pX459-HypaCas9-mR2-RHOA_sgRNA
Plasmid#183877PurposepX459V2.0-HypaCas9 plasmid with mRuby2 reporter and RHOA sgRNA for N-terminal tagging of RhoA in human cells.DepositorAvailable SinceMay 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
PB-CAG-Haunt-EF1a-DsRed
Plasmid#92162PurposeHaunt in trans overexpression plasmidDepositorAvailable SinceMarch 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMXS-IRES-Blast ACSL3
Plasmid#173165PurposeRetroviral vector to express sgRNA resistant human ACSL3DepositorInsertAcyl-CoA Synthetase Long Chain Family Member 3 (ACSL3 Human)
UseRetroviralMutationSynonymous mutations at sgRNA sitesPromoterMMLVAvailable SinceAug. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
EGFP-uTEV1-MAVS-mTA
Plasmid#170999PurposeHiLITR protease with MAVS [R537A] C-terminal tmd targeting information (Mitochondria and ER insertion)DepositorInsertEGFP-uTEV1-MAVS(tmd)[R537A]
UseLentiviral and Synthetic BiologyExpressionMammalianMutationMAVS(tmd)[R537A]PromoterpTRE-tight (TetOn)Available SinceAug. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
EGFP-uTEV1-MAVS-mTA4
Plasmid#171002PurposeHiLITR protease with MAVS [Y536delinsFIVLI] C-terminal tmd targeting information (Mitochondria and ER insertion)DepositorInsertEGFP-uTEV1-MAVS(tmd)[Y536delinsFIVLI]
UseLentiviral and Synthetic BiologyExpressionMammalianMutationMAVS(tmd)[Y536delinsFIVLI]PromoterpTRE-tight (TetOn)Available SinceAug. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMP108 Nanog-Venus-2a-mCherry
Plasmid#159743PurposeNanog targeting vector to insert fluorescent reporter of protein and gene expression levels from endogenous locus in mouse embryonic stem cells. Please see depositor comments for more detail.DepositorAvailable SinceJuly 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
Human_TFAM_dHMGA_pET28
Plasmid#167329PurposeExpresses TFAM dHMGA mutant in bacterial cellsDepositorInsertTFAM (TFAM Human)
Tags6XHisExpressionBacterialMutationTruncation mutant containing linker, HMGB, and C-…PromoterT7Available SinceApril 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDUAL_tacI
Plasmid#155170PurposeBaSP and CuCBP co-expression: tacI promoter controls BaSP expressionDepositorInsertsSucrose Phosphorylase from Bifidobacterium adolescentis (BAD_RS00415 Bifidobacterium adolescentis)
Cellobiose phosphorylase from Cellulomonas uda
UseSynthetic BiologyTagsHis Tag and Strep - Tag IIExpressionBacterialAvailable SinceSept. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDUAL_2_araBAD
Plasmid#155176Purposesee pDUAL_araBAD: exchanged origin of replication (pBR322) and introduced bom siteDepositorInsertsSucrose Phosphorylase from Bifidobacterium adolescentis (BAD_RS00415 Bifidobacterium adolescentis)
Cellobiose phosphorylase from Cellulomonas uda
UseSynthetic BiologyTagsHis Tag and Strep - Tag IIExpressionBacterialAvailable SinceSept. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBICI_2_strong
Plasmid#155168Purposesee pBICI_strong: exchanged origin of replication (pBR322) and introduced bom siteDepositorInsertsSucrose Phosphorylase from Bifidobacterium adolescentis (BAD_RS00415 Bifidobacterium adolescentis)
Cellobiose phosphorylase from Cellulomonas uda
UseSynthetic BiologyTagsHis Tag and Strep - Tag IIExpressionBacterialAvailable SinceSept. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
TB:2xP53_RE:MiniP
Plasmid#133881Purpose2 copies of the p53 DNA binding motifDepositorInsert2 copies of the p53 DNA binding motif
UseLuciferase and Synthetic BiologyExpressionMammalianAvailable SinceJune 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
px459-RhebL1 sgRNA
Plasmid#133769PurposeExpresses Cas9 and human RhebL1 sgRNADepositorInsertRhebL1 sgRNA
UseCRISPRExpressionMammalianPromoterU6Available SinceNov. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
phNFL-P440L
Plasmid#132596PurposeExpresses human NFL containing the Charcot-Marie-Tooth disease type 2E P440L mutationDepositorAvailable SinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
phNFL-N98S
Plasmid#132592PurposeExpresses human NFL containing the Charcot-Marie-Tooth disease type 2E N98S mutationDepositorAvailable SinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAL942
Plasmid#119721Purposeprecursor slow-folding rbsB with the mutations Alanine 248 Threonine, Alanine 188 Cysteine and Glutamic acid 246 CysteineDepositorInsertprecusor slow-folding ribose import binding protein, rbsB, mutations A248T, A118C and E246C
TagsnoneExpressionBacterialMutationAlanine 248 to Threonine, Alanine 188 to Cysteine…PromoterT7Available SinceJan. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBV163
Plasmid#108364PurposeUsed to replace C. glabrata YAP1 promoter with the stronger TDH3 promoter.DepositorInsertKnock-in Cg TDH3 promoter in Cg YAP1 gene
UseReplace c.glabrata yap1 promoter with c. glabrata…PromoterTDH3Available SinceMay 8, 2018AvailabilityAcademic Institutions and Nonprofits only