We narrowed to 9,772 results for: Tec

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Mouse aB crystallin 800bp promoter/AcGFP

Plasmid

#98103

Purpose

Mouse aB crystallin 800bp promoter segment driving GFP expression

Depositor

Mason Posner

Article

Posner et al PeerJ. 2017 Nov 27;5:e4093. doi: 10.7717

Insert

Alpha B-crystallin promoter 800 bp fragment, Mus musculus (Cryab Mouse)

Use

Gfp expressing

Tags

GFP

Promoter

Alpha B-crystallin promoter 800 bp fragment (-833…

Available Since

Sept. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pFastBac1-OSF-TRIMCyp (owl monkey)(K283D, Q287D)

Plasmid

#79036

Purpose

Baculoviral entry vector to produce Bac-to-bac baculoviruses

Depositor

Wesley Sundquist

Article

Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

Insert

OSF-TRIM5Cyp (owl monkey) (K283D, Q287D)

Tags

OneSTrEP-FLAG

Expression

Insect

Mutation

Changed Lys 283 to Asp and Gln 287 to Asp

Promoter

polyhedrin

Available Since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pFRT-TODestFLAGHA_RBPMS_KDR_HindIII

Plasmid

#65662

Purpose

FLAG-HA expression vector with RBPMS carrying mutation to introduce HindIII site and encoding silent mutation to render resistant to siRNA R2 (Farazi et al. 2014)

Depositor

Thomas Tuschl

Article

Teplova et al Q Rev Biophys. 2015 Sep 8:1-11.

Insert

RBPMS (RBPMS Human)

Tags

FLAG HA

Expression

Mammalian

Mutation

introduced HindIII site (final goal to exchange F…

Promoter

CMV, 2x Tet

Available Since

June 4, 2015

Availability

Academic Institutions and Nonprofits only

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GFP-Drosha270-1374delNLS2

Plasmid

#62532

Purpose

N-terminal aa1-269 and nuclear localization signal 2 of Drosha are deleted

Depositor

Bharat Ramratnam

Article

Tang et al Nucleic Acids Res. 2010 Oct;38(19):6610-

Insert

truncated human Drosha mutant (DROSHA Human)

Tags

GFP

Expression

Mammalian

Mutation

deleted amino acids 1-269 and nuclear localizatio…

Available Since

March 20, 2015

Availability

Academic Institutions and Nonprofits only
pTT5-hSDC1-CS2

Plasmid

#52367

Purpose

expresses human Syndecan-1 point mutant at S194A in HEK293-EBNA1 (293E) suspension culture.

Depositor

Gordon Laurie

Article

Zhang et al J Biol Chem. 2013 Apr 26;288(17):12090-1

Insert

SDC1 (SDC1 Human)

Expression

Mammalian

Mutation

R95Q and S194A (according to numbering in Fig. 2A…

Available Since

Dec. 8, 2014

Availability

Academic Institutions and Nonprofits only
pRS406-PHO5-GFP-CERT PH domain EE mutation

Plasmid

#58833

Purpose

Expresses GFP-Tagged and mutated CERT PH domain in yeast

Depositor

Tim Levine

Article

Roy et al J Biol Chem. 2004 Oct 22;279(43):44683-9

Insert

collagen, type IV, alpha 3 binding protein PH domain (CERT1 Human)

Tags

GFP and Myc

Expression

Yeast

Mutation

changed Arginine 43 to Glutamate

Promoter

PHO5

Available Since

Aug. 27, 2014

Availability

Academic Institutions and Nonprofits only
pEGFP-TRAF4(K190R)

Plasmid

#58319

Purpose

EGFP-tagged TRAF4 K190R mutant

Depositor

Ying Zhang

Article

Wang et al J Biol Chem. 2013 Jul 26;288(30):21784-9

Insert

TNF receptor associated factor 4 (Traf4 Mouse)

Tags

GFP

Expression

Mammalian

Mutation

K 190 to R

Promoter

CMV

Available Since

July 28, 2014

Availability

Academic Institutions and Nonprofits only
EGFP-hPMCA2x/b delta6

Plasmid

#47587

Depositor

Emanuel Strehler

Article

Chicka et al J Biol Chem. 2003 May 16;278(20):18464-7

Insert

PMCA2x/b delta6 (ATP2B2 Human)

Tags

EGFP-HA-His

Expression

Mammalian

Mutation

6 C-terminal AA deleted (needed for PDZ binding)

Promoter

CMV

Available Since

Oct. 4, 2013

Availability

Academic Institutions and Nonprofits only
pEGFP-N1 Btsz2

Plasmid

#40068

Depositor

Fernando Martin-Belmonte

Article

Galvez-Santisteban et al Nat Cell Biol. 2012 Jul 22. doi: 10.1038

Insert

Btsz2 (btsz Fly)

Tags

EGFP

Expression

Mammalian

Promoter

CMV promoter

Available Since

Oct. 26, 2012

Availability

Academic Institutions and Nonprofits only
pSG5-FLAG-CaMKK alpha with CaMKKbeta RP domain

Plasmid

#33331

Depositor

Anthony Means

Article

Green et al Cell Signal. 2011 Dec;23(12):2005-12. Ep

Insert

Calcium/Calmodulin dependent protein kinase kinase alpha (Camkk1 Rat)

Tags

FLAG

Expression

Mammalian

Mutation

CaMKKalpha RP domain (167-189) was replaced with …

Promoter

SV40

Available Since

April 10, 2012

Availability

Academic Institutions and Nonprofits only
MAP4K3-GFP d344-873

Plasmid

#21732

Depositor

L. Miguel Martins

Article

Lam et al Proc Natl Acad Sci U S A. 2009 Jul 21;10

Insert

MAP4K3 (MAP4K3 Human)

Tags

GFP

Expression

Mammalian

Mutation

Deletion of the carboxy-terminus, amino-acids 344…

Available Since

June 3, 2010

Availability

Academic Institutions and Nonprofits only
MAP4K3-GFP d1-343

Plasmid

#21733

Depositor

L. Miguel Martins

Article

Lam et al Proc Natl Acad Sci U S A. 2009 Jul 21;10

Insert

MAP4K3 (MAP4K3 Human)

Tags

GFP

Expression

Mammalian

Mutation

Deletion of the amino-terminus, amino-acids 1 to …

Available Since

Dec. 10, 2009

Availability

Academic Institutions and Nonprofits only
pAAV-hsynapsin-DIO-adra2a-shRNA-mCherry

Plasmid

#245063

Purpose

AAV-transgene knocking down adra2a receptor (adrenergic 2a receptor) transcripts cell type-selectively. Using the pPRIME. system, this generates mir30-derived shRNAs and a marker from the same RNA.

Depositor

William Wisden

Article

Anuncibay Soto et al Proc Natl Acad Sci U S A. 2025 Oct 14;12

Insert

AAV-human synapsin promoter-DIO-adra2a-shRNA-mCherry (Adra2a Synthetic, Mouse)

Use

AAV, Cre/Lox, and RNAi

Promoter

human synapsin

Available Since

Oct. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pKC31

Plasmid

#226777

Purpose

Expresses Drosophila UNC-104(R562H) in insect cells

Depositor

Kyoko Chiba

Article

Niwa et al J Cell Sci. 2024 Sep 6:jcs.262017. doi:

Insert

Drosophila unc-104 (R562H) (unc-104 Fly)

Tags

sfGFP and 2xStrepII

Expression

Insect

Mutation

changed Arginine 562 to Histidine (bris mutation)

Available Since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pKSR1-CA3-mRFP1 (C-KSR)

Plasmid

#217754

Purpose

Fluorescent reporter for ceramide (mammalian expression)

Depositor

Paula Nunes-Hasler

Article

Girik et al Int J Mol Sci. 2024 Mar 5;25(5):2996. do

Insert

Kinase suppressor of ras 1 CA3 domain (KSR1 Human)

Tags

mRFP

Expression

Mammalian

Mutation

CA3 domain aa 317-400 plus vector-based linker LE…

Promoter

CMV

Available Since

June 10, 2024

Availability

Academic Institutions and Nonprofits only

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1K245 GLUT1-GFP

Plasmid

#200097

Purpose

human GLUT1 with a single cytosolic lysine (K245) and C-terminal GFP tag in pQCXIP vector

Depositor

Jason MacGurn

Article

Qualls-Histed et al iScience. 2023 Feb 6;26(3):106150. doi:

Insert

GLUT1 (SLC2A1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

K-->R mutations of amino acids 6, 7, 225, 229,…

Promoter

CMV

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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pSems-leader-mEGFP-MBP-TMD(ALA7)

Plasmid

#193051

Purpose

Expression of artificial transmembrane protein tagged with mEGFP and MBP at the plasma membrane. Control construct for single molecule co-localization and co-tracking microscopy.

Depositor

Jacob Piehler

Article

Sotolongo Bellon et al Cell Rep Methods. 2022 Feb 4;2(2):100165

Insert

ALA7-TMD

Tags

Ig k-chain leader sequence, MBP, and mEGFP

Expression

Mammalian

Promoter

CMV

Available Since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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pPHR-mCherry-mHP1alpha-I163A

Plasmid

#181889

Purpose

Soluble light-dependent effector protein (HP1alpha with I163A mutation) for optodroplet formation or recruitment to CIBN localizers,

Depositor

Karsten Rippe

Article

Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

Insert

PHR-mCherry-mHP1alpha-I163A (Cbx5 Mustard Weed, Mouse)

Tags

PHR-mCherry

Expression

Mammalian

Mutation

I163A

Promoter

CMV

Available Since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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pPHR-mCherry-FUSN-mHP1alpha

Plasmid

#181892

Purpose

Soluble light-dependent effector protein (HP1alpha fused to the N-terminal domain of the fused in sarcoma protein (FUSN) ) for optodroplet formation or recruitment to CIBN localizers

Depositor

Karsten Rippe

Article

Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

Insert

PHR-mCherry-FUSN-mHP1alpha (Cbx5 Mustard Weed, Mouse)

Tags

PHR-mCherry

Expression

Mammalian

Promoter

CMV

Available Since

April 20, 2022

Availability

Academic Institutions and Nonprofits only

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pBI-CMV1-mir451~144-CMV2-mir1

Plasmid

#164666

Purpose

Express miR-451~144 and miR-1-1 in mammalian cells

Depositor

David Bartel

Article

Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

Inserts

miR-451~144 (MIR451A Human)
miR-1-1 (MIR1-1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

March 29, 2021

Availability

Academic Institutions and Nonprofits only

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