167,318 results

1. TFORF2118

   Plasmid

   #141929

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   TBPL1 (TBPL1 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. L4440_BioBrick-sgRNA

   Plasmid

   #177783

   Purpose

   Overexpression of sgRNAs in E. coli HT115, sgRNA overexpression scaffold; for ingestion by C. elegans

   Depositor

   Michael Ristow

   Article

   Fischer et al J Biol Chem. 2022 May 27:102085. doi: 10

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   June 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCDNA3_cAMPFIRE-M

   Plasmid

   #182280

   Purpose

   Mammalian expression of the cAMP sensor cAMPFIRE-M under a CMV promotor.

   Depositor

   Haining Zhong

   Article

   Massengill et al Nat Methods. 2022 Nov;19(11):1461-1471.

   Insert

   cAMPFIRE-M (cAMP sensor)
   

   Expression

   Mammalian

   Available Since

   March 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. YES1 gRNA (BRDN0001148961)

   Plasmid

   #77967

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human YES1

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   YES1 (YES1 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. YES1 gRNA (BRDN0001145317)

   Plasmid

   #77965

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human YES1

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   YES1 (YES1 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. YES1 gRNA (BRDN0001487120)

   Plasmid

   #77966

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human YES1

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   YES1 (YES1 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pUC57-4eCOL2A1

   Plasmid

   #97211

   Purpose

   For cloning of a COL2A1-based, chondrogenesis-responsive promoter, and subcloning this insert into new vectors

   Depositor

   Ryan Porter

   Article

   De La Vega et al Tissue Eng Part C Methods. 2019 Feb 6. d

   Insert

   4 repeats of COL2A1 intronic enhancer (+2126/+2174) upstream of core promoter (-164/+37) (COL2A1 Human)
   

   Use

   Vector is designed for cloning and generation of …

   Promoter

   COL2A1 regulatory elements

   Available Since

   Oct. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pADH147

   Plasmid

   #90991

   Purpose

   NAT-marked "empty" gRNA construct for use in gRNA expression cassette stitching PCR. Use with pADH110 to generate target-specific part 2 of 2 for C.alb HIS1-FLP CRISPR system.

   Depositor

   Aaron Hernday

   Article

   Nguyen et al mSphere. 2017 Apr 26;2(2):e00149-17. doi

   Insert

   gRNA conserved, FRT, DS_HIS1
   

   Use

   CRISPR

   Available Since

   June 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. SpCas9-CBE6c-V106W

   Plasmid

   #215826

   Purpose

   Express CBE6c V106W (with SpCas9) in mammalian cells

   Depositor

   David Liu

   Article

   Zhang et al Nat Commun. 2024 Feb 24;15(1):1697. doi:

   Insert

   CBE6c V106W-SpCas9-2xUGI (cas9 )
   

   Use

   In vitro transcription template

   Available Since

   March 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLenti-hSyn-nucGFP

    Plasmid

    #140190

    Purpose

    Overexpression of GFP with a nuclear localization sequence, under the human synapsin promoter

    Depositor

    Lorenz Studer

    Article

    Miller et al Cell Stem Cell. 2013 Dec 5;13(6):691-705

    Insert

    GFP-NLS
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    July 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBbA8k-RFP

    Plasmid

    #35273

    Depositor

    Jay Keasling

    Article

    Lee et al J Biol Eng. 2011 Sep 20;5:12.

    Insert

    RFP
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    BAD

    Available Since

    Feb. 16, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCEP4-191084-gp160

    Plasmid

    #123234

    Purpose

    Mammalian expression plasmid for Env from the 191084 B7-19 HIV-1 isolate

    Depositor

    Erik Procko

    Article

    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

    Insert

    HIV-1 (191084 B7-19) Env
    

    Tags

    CD5 leader peptide

    Expression

    Mammalian

    Mutation

    Codon-optimized synthetic gene

    Promoter

    CMV

    Available Since

    April 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDONR223_IRGM_WT

    Plasmid

    #82134

    Purpose

    Gateway Donor vector containing IRGM , part of the Target Accelerator Plasmid Collection.

    Depositor

    David Root, Jesse Boehm, William Hahn

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    IRGM (IRGM Human)
    

    Use

    Gateway entry vector

    Mutation

    WT

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHM130

    Plasmid

    #225529

    Purpose

    Encodes destablized GFP under IBRE4 promoter, and constitutive BFP

    Depositor

    Jared Toettcher

    Article

    McNamara et al Nat Cell Biol. 2024 Oct 2. doi: 10.1038/

    Inserts

    GFP
    BFP
    

    Available Since

    Nov. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLV-PLXNB2

    Plasmid

    #86237

    Purpose

    Lentivirus for expression of human Plexin-B2

    Depositor

    Roland Friedel

    Article

    Huang et al Commun Biol. 2021 Jan 29;4(1):145. doi:

    Insert

    Plexin-B2 (PLXNB2 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    full length PLXNB2, CRISPR resistant

    Promoter

    human PGK promoter

    Available Since

    Jan. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. EMMA toolkit

    Plasmid Kit

    #1000000119

    Purpose

    Used for the construction of a variety of mammalian expression vectors in a one-pot, one-step Golden Gate reaction; compatible with automated production

    Depositor

    Yizhi Cai

    Available Since

    March 1, 2018

    Availability

    Academic Institutions and Nonprofits only

17. pCAS9-mCherry-Frame +0

    Plasmid

    #66939

    Purpose

    CRISPaint frame selector +0

    Depositor

    Veit Hornung

    Article

    Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

    Insert

    gRNA frame +0
    

    Available Since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. AAV-EF1a-BbChT

    Plasmid

    #45186

    Depositor

    Joshua Sanes, Dawen Cai

    Article

    Cai et al Nat Methods. 2013 May 5;10(6):540-7. doi

    Has Service

    AAV9

    Insert

    pAAV-Ef1a-Brainbow/mCherry/mTFP-WPRE
    

    Use

    AAV; Adeno-associated viral

    Expression

    Mammalian

    Promoter

    human EF1a

    Available Since

    Nov. 14, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET His10 MBP Asn10 TEV LIC cloning vector (2CT-10)

    Plasmid

    #55209

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Tags

    10xAsn, 10xHis, MPB, and TEV cleavage site

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCI-Neo HA-hSnoN

    Plasmid

    #10908

    Depositor

    Bob Weinberg

    Article

    Sun et al Proc Natl Acad Sci U S A. 1999 Oct 26. 9

    Insert

    SnoN (SKIL Human)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    April 4, 2006

    Availability

    Academic Institutions and Nonprofits only