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pDH009

Plasmid

#157644

Purpose

sMHT-113 fused to GRX2

Depositor

Jonathan Silberg

Article

Fulk et al ACS Synth Biol. 2020 Nov 20;9(11):3104-3

Inserts

sMHT(1-113)-GRX2
GRX2-sMHT(114-230)

Expression

Bacterial

Available Since

May 20, 2021

Availability

Academic Institutions and Nonprofits only

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pDH011

Plasmid

#157646

Purpose

sMHT-113 fused to FKBP/FRB

Depositor

Jonathan Silberg

Article

Fulk et al ACS Synth Biol. 2020 Nov 20;9(11):3104-3

Inserts

sMHT(1-113)-FKBP
FRB-sMHT(114-230)

Expression

Bacterial

Available Since

May 20, 2021

Availability

Academic Institutions and Nonprofits only

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pDH012

Plasmid

#157647

Purpose

Constitutive sMHT-113-SYNZIP expression

Depositor

Jonathan Silberg

Article

Fulk et al ACS Synth Biol. 2020 Nov 20;9(11):3104-3

Inserts

sMHT(1-113)-SYNZIP17
SYNZIP18-sMHT(114-230)

Expression

Bacterial

Available Since

May 20, 2021

Availability

Academic Institutions and Nonprofits only

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PAJ408

Plasmid

#104150

Purpose

dCas9 plasmid expressing full length mKate2 mRNA without RBS

Depositor

Alfonso Jaramillo

Article

Galizi et al CRISPR J. 2020 Oct;3(5):398-408. doi: 10

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Promoter

pLtetO-1

Available Since

Feb. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pJM597 ZF(2/6)x3 mKate2 in TUPV1

Plasmid

#161531

Purpose

Inducible expression of mKate2 under the ZF(2/6)x3 promoter

Depositor

Joshua Leonard

Article

Muldoon et al Sci Adv. 2021 Feb 19;7(8). pii: 7/8/eabe

Insert

mKate2

Use

Synthetic Biology

Expression

Mammalian

Promoter

ZF(2/6)x3

Available Since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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Prham-M.PstI-p15A-aadA

Plasmid

#149340

Purpose

expresses M.PstI DNA methyltransferase in E. coli

Depositor

Ichiro Matsumura

Article

Matsumura et al PeerJ. 2020 Sep 11;8:e9841. doi: 10.7717

Insert

M.,PstI

Expression

Bacterial

Promoter

rhamnose

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pFA6a-SRS-FLAG:HIS3MX6

Plasmid

#134447

Purpose

PCR-based C-terminal SRS-FLAG tag

Depositor

Robert Tomko

Article

Nemec et al Yeast. 2020 May 13. doi: 10.1002/yea.347

Insert

SRS-FLAG

Use

Pcr-based tagging in yeast

Available Since

May 14, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pFA6a-SRS-FLAG:kanMX6

Plasmid

#134446

Purpose

PCR-based C-terminal SRS-FLAG tag

Depositor

Robert Tomko

Article

Nemec et al Yeast. 2020 May 13. doi: 10.1002/yea.347

Insert

SRS-FLAG

Use

Pcr-based tagging in yeast

Available Since

May 14, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pFD-AI(TVMV)

Plasmid

#137111

Purpose

iFLinkC Linker cloning

Depositor

Viktor Stein

Article

Grawe et al Nucleic Acids Res. 2020 Jan 11. pii: 570

Insert

AI(TVMV)

Use

Synthetic Biology

Available Since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pFGL1269R

Plasmid

#118994

Purpose

Marker fusion of Bar:mCherry (without start codon)

Depositor

Naweed Naqvi

Article

Yadav et al MBio. 2019 Jul 30;10(4). pii: mBio.01581

Insert

Bar:mCherry

Use

Fungal expression

Promoter

promoter less

Available Since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pFGL1259

Plasmid

#118996

Purpose

GFP (without start and stop codons) in pFGL1010

Depositor

Naweed Naqvi

Article

Yadav et al MBio. 2019 Jul 30;10(4). pii: mBio.01581

Insert

eGFP

Use

Fungal expression

Promoter

promoter less

Available Since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pNIC28-BpWcbI

Plasmid

#125591

Purpose

Overexpression plasmid for production of WcbI from Burkholderia pseudomallei

Depositor

Nicholas Harmer

Article

Vivoli et al IUCrJ. 2013 Oct 18;1(Pt 1):28-38. doi: 1

Insert

BpWcbI (wcbI Burkholderia pseudomallei)

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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pClodAcytCh-GFP-P4M

Plasmid

#113346

Purpose

Bacterial expression of Cherry and GFP-SidM-P4M domain (2 copies)

Depositor

Sanford Simon

Article

Botero et al Sci Adv. 2019 Mar 27;5(3):eaat4872. doi:

Inserts

Cherry
GFP-SidM-P4M domain (2 copies of P4M in tandem)

Expression

Bacterial

Promoter

ProD and proD

Available Since

April 8, 2019

Availability

Academic Institutions and Nonprofits only

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pBHDuet50K

Plasmid

#118399

Purpose

analysis of cis-splicing by mini inteins

Depositor

Hideo Iwai

Article

Iwai et al J Mol Biol. 2017 Dec 8;429(24):3942-3956

Insert

MvuTFIIB mini intein

Expression

Bacterial

Mutation

incorporation of oligonucleotide during cloning

Available Since

Dec. 3, 2018

Availability

Academic Institutions and Nonprofits only

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AAEL010313 SPR

Plasmid

#115385

Purpose

Expresses Ae. aegypti receptor in mammalian cells

Depositor

Leslie Vosshall

Article

Duvall et al Cell. 2019 Feb 7;176(4):687-701.e5. doi:

Insert

G-protein coupled receptor daf-37

Expression

Mammalian

Available Since

Sept. 19, 2018

Availability

Academic Institutions and Nonprofits only

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AAEL007405 SP R

Plasmid

#115393

Purpose

Expresses Ae. aegypti receptor in mammalian cells

Depositor

Leslie Vosshall

Article

Duvall et al Cell. 2019 Feb 7;176(4):687-701.e5. doi:

Insert

AAEL007405 SP R (SPR Aedes aegypti)

Expression

Mammalian

Available Since

Sept. 19, 2018

Availability

Academic Institutions and Nonprofits only

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AAEL012796

Plasmid

#115383

Purpose

Expresses Ae. aegypti receptor in mammalian cells

Depositor

Leslie Vosshall

Article

Duvall et al Cell. 2019 Feb 7;176(4):687-701.e5. doi:

Insert

AAEL012796 (LOC5576812 Aedes aegypti)

Expression

Mammalian

Available Since

Sept. 19, 2018

Availability

Academic Institutions and Nonprofits only

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AAEL007169 AstA R

Plasmid

#115400

Purpose

Expresses Ae. aegypti receptor in mammalian cells

Depositor

Leslie Vosshall

Article

Duvall et al Cell. 2019 Feb 7;176(4):687-701.e5. doi:

Insert

AAEL007169 AstA R (AaeL_AAEL007169 )

Expression

Mammalian

Available Since

Sept. 6, 2018

Availability

Academic Institutions and Nonprofits only

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AAEL006283 MS R

Plasmid

#115386

Purpose

Expresses Ae. aegypti receptor in mammalian cells

Depositor

Leslie Vosshall

Article

Duvall et al Cell. 2019 Feb 7;176(4):687-701.e5. doi:

Insert

AAEL006283 MS R (LOC5567691 Aedes aegypti)

Expression

Mammalian

Available Since

Sept. 6, 2018

Availability

Academic Institutions and Nonprofits only

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puC19 preACT1(del6) short BP-3'SS

Plasmid

#111333

Purpose

T7 transcription

Depositor

Christine Guthrie

Article

Villa et al Genes Dev. 2005 Aug 15;19(16):1894-904.

Insert

ACT1

Expression

Bacterial

Mutation

shorter branch to 3' splice site distance

Available Since

Aug. 28, 2018

Availability

Academic Institutions and Nonprofits only

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