We narrowed to 91,130 results for: MAL

Showing: 6861 - 6880 of 91130 results

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EB3-3xmNeonGreen-N1

Plasmid

#129605

Purpose

In vivo visualization of microtuble plus ends (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

Insert

EB3

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pMaCTag-P34

Plasmid

#120030

Purpose

Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with 3xFLAG

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

3xFLAG

Use

Pcr template

Tags

3xFLAG

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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mNeonGreen-UtrCH

Plasmid

#98879

Purpose

In vivo visualization of actin cytoskeleton (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

Insert

UtrCH

Tags

mNeonGreen

Expression

Mammalian

Mutation

*(see below)

Promoter

CMV

Available Since

March 7, 2019

Availability

Academic Institutions and Nonprofits only

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eMagA-mCherry-Mito

Plasmid

#162251

Purpose

Blue-light dependent heterodimerization system

Depositor

Pietro De Camilli

Article

Benedetti et al Elife. 2020 Nov 11;9. pii: 63230. doi: 1

Insert

eMagA-mCherry-OMP25

Expression

Mammalian

Promoter

beta-actin promoter

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pCX-2A-2A

Plasmid

#74670

Purpose

based on pCX-C1 with F2A1/F2A2 inserted

Depositor

Roberto Giovannoni

Article

De Giorgi et al Plasmid. 2015 May;79:22-9. doi: 10.1016/

Type

Empty backbone

Expression

Mammalian

Promoter

pCAGGS

Available Since

Feb. 14, 2017

Availability

Academic Institutions and Nonprofits only

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p2T-CMV-evoCjCas9-BlastR

Plasmid

#194059

Purpose

Mammalian expression of evoCjCas9 nuclease

Depositor

Gerald Schwank

Article

Schmidheini et al Nat Chem Biol. 2023 Sep 21. doi: 10.1038

Insert

evoCjCas9

Use

CRISPR

Expression

Mammalian

Available Since

Sept. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pCS2_hMGFP-60A

Plasmid

#200393

Purpose

A plasmid IVT template containing a humanized Monster Green Fluorescent Protein (hMGFP) CDS followed by a template-encoded poly(A) tail. Linearization at an Esp3i site.

Depositor

Xiao Wang

Article

Aditham et al ACS Chem Biol. 2022 Dec 16;17(12):3352-3

Insert

monster GFP

Expression

Mammalian

Available Since

May 12, 2023

Availability

Academic Institutions and Nonprofits only

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pSF4 CMV intron renilla TRICK CTE polyA

Plasmid

#84443

Purpose

TRICK reporter mRNA

Depositor

Jeffrey Chao

Article

Halstead et al Science. 2015 Mar 20;347(6228):1367-671.

Insert

Renill-TRICK

Use

Luciferase; Frt

Expression

Mammalian

Promoter

Tet-CMV

Available Since

Nov. 29, 2016

Availability

Academic Institutions and Nonprofits only

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pCMV-Cas9-NRCH

Plasmid

#136926

Purpose

Expresses Cas9-NRCH in mammalian cells

Depositor

David Liu

Article

Miller et al Nat Biotechnol. 2020 Apr;38(4):471-481.

Insert

Cas9-NRCH

Use

CRISPR

Expression

Mammalian

Mutation

see manuscript

Promoter

CMV

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pCS2+ GFP-2xESP3I-IRES-mCherry

Plasmid

#230996

Purpose

Protein stability reporter construct for transient expression in mammalian cells. Stability of N-terminal GFP-fusion protein can be assessed by flow cytometry by normalizing to mCherry expression.

Depositor

Michael Rape

Article

Manford et al Cell. 2020 Oct 1;183(1):46-61.e21. doi:

Type

Empty backbone

Tags

AcGFP1

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-Cas9-NRTH

Plasmid

#136925

Purpose

Expresses Cas9-NRTH in mammalian cells

Depositor

David Liu

Article

Miller et al Nat Biotechnol. 2020 Apr;38(4):471-481.

Insert

Cas9-NRTH

Use

CRISPR

Expression

Mammalian

Mutation

see manuscript

Promoter

CMV

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pJM456 EF1alpha EBFP2-P2A-BlastR (TUPV7)

Plasmid

#139236

Purpose

mMoClo TUPV7, with EF1alpha promoter and EBFP2-P2A-BlastR in the MCS

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

EBFP2-P2A-Blasticidin S Deaminase

Use

Synthetic Biology

Expression

Mammalian

Promoter

EF1alpha

Available Since

Feb. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pRK5-myc-PAT1

Plasmid

#88856

Purpose

myc-TAG overexpression

Depositor

David Sabatini

Article

Zoncu et al Science. 2011 Nov 4;334(6056):678-83.

Insert

SLC36A1 (SLC36A1 Human)

Tags

myc

Expression

Mammalian

Available Since

April 28, 2017

Availability

Academic Institutions and Nonprofits only

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pMaCTag-P28

Plasmid

#120039

Purpose

Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with 3xHA

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

3xHA

Use

Pcr template

Tags

3xHA

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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Tad-CBE(GCC3)

Plasmid

#235078

Purpose

Expresses Tad-CBE(GCC3) in mammalian cells.

Depositor

Weixin Tang

Article

Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

Insert

Tad-CBE(GCC3)-SpCas9 D10A

Use

CRISPR

Tags

2xUGI, bpNLS and bpNLS

Expression

Mammalian

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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Tad-CBE(GCG4)

Plasmid

#235080

Purpose

Expresses Tad-CBE(GCG4) in mammalian cells.

Depositor

Weixin Tang

Article

Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

Insert

Tad-CBE(GCG4)-SpCas9 D10A

Use

CRISPR

Tags

2xUGI, bpNLS and bpNLS

Expression

Mammalian

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Ras-LOCKR-PL Key

Plasmid

#216503

Purpose

Ras activity dependent proximity labeler, Key only, untargeted

Depositor

David Baker

Article

Zhang et al Nat Biotechnol. 2024 Jan 25. doi: 10.103

Insert

Ras-LOCKR-PL Key

Expression

Mammalian

Available Since

April 9, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Ras-LOCKR-PL Cage

Plasmid

#216502

Purpose

Ras activity dependent proximity labeler, Cage only, untargeted

Depositor

David Baker

Article

Zhang et al Nat Biotechnol. 2024 Jan 25. doi: 10.103

Insert

Ras-LOCKR-PL Cage

Expression

Mammalian

Available Since

April 9, 2024

Availability

Academic Institutions and Nonprofits only

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pMLM3705

Plasmid

#47754

Purpose

Expresses mammalian cell codon-optimized dCas9-VP64

Depositor

Keith Joung

Article

Maeder et al Nat Methods. 2013 Jul 25. doi: 10.1038/n

Insert

codon optimized dCas9-VP64

Use

CRISPR

Tags

3x FLAG, NLS, and VP64

Expression

Mammalian

Mutation

D10A and H840A mutations in Cas9 (catalytically i…

Promoter

CMV

Available Since

Aug. 27, 2013

Availability

Academic Institutions and Nonprofits only

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pMXs-hNANOG (Plath)

Plasmid

#18115

Depositor

Kathrin Plath

Article

Lowry et al Proc Natl Acad Sci U S A. 2008 Feb 26. 1

Insert

Nanog homeobox (NANOG Human)

Use

Retroviral

Expression

Mammalian

Available Since

June 11, 2008

Availability

Academic Institutions and Nonprofits only

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