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pLVXPuro-mGFP-Sec23B

Plasmid

#66598

Purpose

Expresses Sec23B as a fusion with mGFP

Depositor

David Stephens

Article

Hughes et al J Cell Sci. 2010 Dec 1;123(Pt 23):4032-8

Insert

Sec23B (SEC23B Human)

Use

Lentiviral

Tags

mEGFP

Expression

Mammalian

Available Since

July 8, 2015

Availability

Academic Institutions and Nonprofits only

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yWL234 MCP-GFP-PABPC1*

Plasmid

#197054

Purpose

pUBC-NLS-HA-2xMCP-1xGFP-PABPC1*

Depositor

Robert Singer

Article

Li et al Nat Methods. 2022 Dec;19(12):1558-1562.

Insert

pubc-nls-ha-MCP-GFP-PABPC1*

Use

Lentiviral

Expression

Mammalian

Promoter

ubc

Available Since

March 24, 2023

Availability

Academic Institutions and Nonprofits only

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pSLHDR01_mCherry_tCD19

Plasmid

#123661

Purpose

Construction of 1st donor for scarless genome editing

Depositor

Matthew Porteus

Article

Ikeda et al Nat Methods. 2018 Dec;15(12):1045-1047.

Type

Empty backbone

Use

Mouse Targeting; Human targeting

Available Since

April 17, 2019

Availability

Academic Institutions and Nonprofits only

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pNLS-TetR-GFP

Plasmid

#103838

Purpose

Flourescent marker for tetO arrays

Depositor

Karsten Rippe

Article

Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

Insert

NLS-TetR (tetR )

Tags

EGFP

Expression

Mammalian

Mutation

TetR: A4G (M2V)

Promoter

CMV

Available Since

Dec. 5, 2017

Availability

Academic Institutions and Nonprofits only

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pJB06

Plasmid

#190480

Purpose

Base Cas9 expression plasmid for 2-plasmid C. difficile mutagenesis system

Depositor

Joseph Sorg

Article

Brehm et al Microbiol Resour Announc. 2022 Dec 15;11

Inserts

Cas9
XylR

Use

E. coli / b. subtilis - c. difficile shuttle vect…

Promoter

xylR-driven promoter

Available Since

Oct. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pJB07

Plasmid

#190481

Purpose

Targeting plasmid for 2-plasmid C. difficile mutagenesis system

Depositor

Joseph Sorg

Article

Brehm et al Microbiol Resour Announc. 2022 Dec 15;11

Inserts

Upstream & Downstream pyrE deletion region
gRNA

Use

E. coli / b. subtilis - c. difficile shuttle vect…

Available Since

Sept. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pNH-NanoLuc

Plasmid

#173075

Purpose

Expression vector for E. coli producing target protein with N-terminal Nanoluciferase fusion

Depositor

Lari Lehtiö

Article

Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

Type

Empty backbone

Expression

Bacterial

Available Since

Nov. 15, 2021

Availability

Academic Institutions and Nonprofits only

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hAur-A D274A

Plasmid

#8511

Depositor

Joan Ruderman

Article

Crane et al J Cell Sci 2004 Dec 1;117(Pt 25):5975-83

Insert

hAur-A (AURKA Human)

Use

Retroviral

Expression

Mammalian

Mutation

D274A, catalytically dead mutant

Available Since

Dec. 7, 2005

Availability

Academic Institutions and Nonprofits only

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YIplac211-ABP1-iGFPx6

Plasmid

#177099

Purpose

Expresses Abp1-iGFPx6 in yeast cells

Depositor

Benjamin Glick

Article

Casler et al J Cell Biol. 2022 Jan 3;221(1). pii: 212

Insert

ABP1 (ABP1 Budding Yeast)

Expression

Yeast

Available Since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pAquaN1

Plasmid

#42888

Depositor

Fabienne Merola

Article

Erard et al Mol Biosyst. 2013 Feb 2;9(2):258-67. doi

Insert

Aquamarine

Expression

Mammalian

Mutation

T66S and H149G

Available Since

March 14, 2013

Availability

Academic Institutions and Nonprofits only

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pEB1-moxGFP

Plasmid

#103984

Purpose

Plasmid encoding moxGFP

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

moxGFP

Use

Low copy

Expression

Bacterial

Mutation

Mutations relative to wild-type GFP (S30R, Y39N, …

Promoter

proC

Available Since

Dec. 13, 2017

Availability

Academic Institutions and Nonprofits only

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yWL229 MCP-GFP-eRF3

Plasmid

#197053

Purpose

pUBC-NLS-HA-2xMCP-1xGFP-eRF3

Depositor

Robert Singer

Article

Li et al Nat Methods. 2022 Dec;19(12):1558-1562.

Insert

pubc-nls-ha-MCP-GFP-eRF3

Use

Lentiviral

Expression

Mammalian

Promoter

ubc

Available Since

March 9, 2023

Availability

Academic Institutions and Nonprofits only

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FlagMBNL3

Plasmid

#96901

Purpose

expression of Flag-tagged human MBNL3

Depositor

Thomas Cooper

Article

Orengo et al Nucleic Acids Res. 2006;34(22):e148. Epu

Insert

human MBNL3 cDNA (MBNL3 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pEB1-mVenus ME

Plasmid

#103989

Purpose

Plasmid encoding mVenus ME

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

mVenus ME

Use

Low copy

Expression

Bacterial

Mutation

Mutations relative to wild-type GFP (F46L, F64L, …

Promoter

proC

Available Since

Dec. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pN1-CMV-Aff6_V18FdN-IntC-mCherryC-T2A-EGFP (C2)

Plasmid

#210725

Purpose

NIR optogenetic module for conditional protein splicing of split mCherry (C-part)

Depositor

Vladislav Verkhusha

Article

Karasev et al J Mol Biol. 2023 Dec 15;435(24):168360.

Insert

C2

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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yWL268 MCP-GFP

Plasmid

#197052

Purpose

pUBC-NLS-HA-2xMCP-2xGFP

Depositor

Robert Singer

Article

Li et al Nat Methods. 2022 Dec;19(12):1558-1562.

Insert

pubc-nls-ha-MCP-2xGFP

Use

Lentiviral

Expression

Mammalian

Promoter

ubc

Available Since

March 31, 2023

Availability

Academic Institutions and Nonprofits only

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pBS_36x-601_MA1+MA2+MA3

Plasmid

#114363

Purpose

36x Widom 601 nucleosome sequence

Depositor

Scot Wolfe

Article

Swygert et al Proc Natl Acad Sci U S A. 2018 Dec 4;115

Insert

36X601-MA1_2_3

Use

Unspecified

Expression

Mammalian

Available Since

March 19, 2019

Availability

Academic Institutions and Nonprofits only

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p1177-X1-8

Plasmid

#38150

Depositor

David Waugh

Article

Austin et al Protein Expr Purif. 2011 May;77(1):53-61

Insert

Carboxypeptidase A

Tags

HHHHHHRR

Expression

Insect

Promoter

polyhedron

Available Since

Oct. 12, 2012

Availability

Academic Institutions and Nonprofits only

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pME tdTomato

Plasmid

#135202

Purpose

Red fluorescent tag

Depositor

David Tobin

Article

Oehlers et al Nature. 2015 Jan 29;517(7536):612-5. doi

Insert

tdTomato

Use

Synthetic Biology

Available Since

Dec. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pmyc-MOV10

Plasmid

#10977

Depositor

Thomas Tuschl

Article

Meister et al Curr Biol. 2005 Dec 6;15(23):2149-55. do

Insert

MOV10 (MOV10 Human)

Tags

myc

Expression

Mammalian

Available Since

Feb. 13, 2006

Availability

Academic Institutions and Nonprofits only

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pCMV-EPN-24

Plasmid

#79939

Purpose

PH-Late1-I3-01-myc

Depositor

Neil King

Article

Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

Insert

EPN-24

Expression

Bacterial and Mammalian

Available Since

Feb. 10, 2017

Availability

Academic Institutions and Nonprofits only

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pBES1-LacOx50-blast

Plasmid

#210221

Purpose

Integrates 50xLacO repeats upstream of the BES1 promoter with a blasticidin gene downstream of the promoter.

Depositor

Calvin Tiengwe

Article

Budzak et al Mol Biochem Parasitol. 2023 Dec;256:1115

Insert

50xLacO repeats derived from: PMID: 12864855.

Available Since

Nov. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pEB1-mYPet

Plasmid

#103988

Purpose

Plasmid encoding mYPet

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

mYPet

Use

Low copy

Expression

Bacterial

Mutation

Mutations relative to wild-type GFP (F46L, I47L, …

Promoter

proC

Available Since

Dec. 14, 2017

Availability

Academic Institutions and Nonprofits only

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GST-PRMT6

Plasmid

#34700

Depositor

Steven Clarke

Article

Frankel et al J Biol Chem. 2002 Feb 1;277(5):3537-43.

Insert

PMRT6

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

Jan. 27, 2012

Availability

Academic Institutions and Nonprofits only
pmAmetrine1.2

Plasmid

#42171

Depositor

Robert Campbell

Article

Ding et al Anal Chem. 2011 Dec 15;83(24):9687-93. d

Insert

mAmetrine1.2

Tags

6His

Expression

Bacterial

Promoter

araBAD

Available Since

Feb. 6, 2013

Availability

Academic Institutions and Nonprofits only

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TARG1-c002

Plasmid

#173108

Purpose

Expression of recombinant fusion protein in E. coli

Depositor

Lari Lehtiö

Article

Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

Insert

TARG1

Tags

mCerulean

Expression

Bacterial

Mutation

Full length protein

Available Since

Nov. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pMUT2-pTcpP-mCherry

Plasmid

#192861

Purpose

pMUT2 based vector for sensing human primary bile acids in E. coli Nissle 1917

Depositor

Morten Sommer

Article

Vaaben et al ACS Synth Biol. 2022 Dec 16;11(12):4184-

Insert

TcpP-CadC fusion protein and cognate promoter inducible by human primary bile acids

Expression

Bacterial

Available Since

April 13, 2023

Availability

Academic Institutions and Nonprofits only

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pET-6xHis-GFP(+18)

Plasmid

#199162

Purpose

used to express a supercharged GFP variant in E. coli

Depositor

Allie Obermeyer

Article

Yeong et al ACS Cent Sci. 2020 Dec 23;6(12):2301-231

Insert

GFP(+18)

Tags

MGHHHHHHGGA

Mutation

E16R, D29K, E42K, E44K, D86K, D112K, T115K, D127R…

Promoter

T7

Available Since

May 10, 2023

Availability

Academic Institutions and Nonprofits only

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pRST11B-AS3_4

Plasmid

#102789

Purpose

Expresses tryptophanyl tRNA synthetase (ScWRS) and supressor tryptophanyl tRNA

Depositor

Andrew Ellington

Article

Abil et al Nat Protoc. 2017 Dec;12(12):2493-2512. d

Inserts

tryptophanyl tRNA synthetase (WRS1 Budding Yeast)
suppressor tRNA

Expression

Bacterial

Available Since

July 3, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pET-6xHis-GFP(+12)

Plasmid

#199161

Purpose

used to express a supercharged GFP variant in E. coli

Depositor

Allie Obermeyer

Article

Yeong et al ACS Cent Sci. 2020 Dec 23;6(12):2301-231

Insert

GFP(+12)

Tags

MGHHHHHHGGA

Mutation

E16R, E44K, D86K, D112K, T115K, D127R, I138R, E15…

Promoter

T7

Available Since

May 10, 2023

Availability

Academic Institutions and Nonprofits only

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