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pJW1674

Plasmid

#112048

Purpose

CEN/ARS plasmid for GAL-inducible SWP1-V5-TEV-EGFP expression marked with URA

Depositor

Jonathan Weissman

Article

Costa et al Science. 2018 Jan 18. pii: science.aar36

Insert

SWP1

Tags

V5-TEV-EGFP

Expression

Yeast

Promoter

GAL1

Availability

Academic Institutions and Nonprofits only

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pAM003

Plasmid

#170007

Purpose

Encodes PmgtC-sfgfp reporter and TetR-controlled expression of S. Typhimurium PhoP

Depositor

Jeffrey Tabor

Article

Brink et al bioRxiv

Inserts

Superfolder GFP
PhoP

Use

Synthetic Biology

Expression

Bacterial

Promoter

PLTetO-1 and PmgtC

Availability

Academic Institutions and Nonprofits only

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pAM004

Plasmid

#170008

Purpose

Encodes PugtL-sfgfp reporter and TetR-controlled expression of S. Typhimurium PhoP

Depositor

Jeffrey Tabor

Article

Brink et al bioRxiv

Inserts

Superfolder GFP
PhoP

Use

Synthetic Biology

Expression

Bacterial

Promoter

PLTetO-1 and PugtL

Availability

Academic Institutions and Nonprofits only

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pAM006

Plasmid

#170010

Purpose

Encodes PpagC-sfgfp reporter and TetR-controlled expression of S. Typhimurium PhoP

Depositor

Jeffrey Tabor

Article

Brink et al bioRxiv

Inserts

Superfolder GFP
PhoP

Use

Synthetic Biology

Expression

Bacterial

Promoter

PLTetO-1 and PpagC

Availability

Academic Institutions and Nonprofits only

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pAM007

Plasmid

#170011

Purpose

Encodes PslyB-sfgfp reporter and TetR-controlled expression of S. Typhimurium PhoP

Depositor

Jeffrey Tabor

Article

Brink et al bioRxiv

Inserts

Superfolder GFP
PhoP

Use

Synthetic Biology

Expression

Bacterial

Promoter

PLTetO-1 and PslyB

Availability

Academic Institutions and Nonprofits only

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hBax C3-EGFP

Plasmid

#19741

Depositor

Richard Youle

Article

Nechushtan et al EMBO J. 1999 May 4. 18(9):2330-41.

Insert

Bax (BAX Human)

Tags

GFP

Expression

Mammalian

Mutation

none

Available Since

Nov. 10, 2008

Availability

Academic Institutions and Nonprofits only

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pEHAC1-HA-BirAonly

Plasmid

#232584

Purpose

Express HA-BirA

Depositor

Sagar Bhogaraju

Article

Mukhopadhyay et al Sci Adv. 2024 Aug 9;10(32):eadp3000. doi

Insert

BirA

Tags

HA

Expression

Mammalian

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA5-(-2)AP-Ub

Plasmid

#232582

Purpose

Express Ubiquitin tagged with a modified Acceptor Peptide (AP)

Depositor

Sagar Bhogaraju

Article

Mukhopadhyay et al Sci Adv. 2024 Aug 9;10(32):eadp3000. doi

Insert

Ubiquitin

Tags

AP

Expression

Mammalian

Available Since

March 12, 2025

Availability

Academic Institutions and Nonprofits only

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pT3TS-Tol2

Plasmid

#31831

Depositor

Stephen Ekker

Article

Balciunas et al PLoS Genet. 2006 Nov 10;2(11):e169. Epub

Insert

Tol2 Transposase

Use

Cloning vector

Promoter

Lac

Available Since

Aug. 29, 2011

Availability

Academic Institutions and Nonprofits only

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pRVdG-N-P-M-nucBFP-EGFP-L

Plasmid

#52485

Purpose

Expresses nuclear mTagBFP and EGFP

Depositor

Sebastian Seung

Article

Wickersham et al Nat Commun. 2013;4:2332. doi: 10.1038/nc

Inserts

nuclear mTagBFP
EGFP

Use

Rabies virus

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pBlueBac human APC2

Plasmid

#11425

Depositor

Marc Kirschner

Article

Yu et al Science. 1998 Feb 20. 279(5354):1219-22.

Insert

APC2 (ANAPC2 Human)

Use

Baculoviral

Available Since

April 20, 2006

Availability

Academic Institutions and Nonprofits only
pCMV-Tol2

Plasmid

#31823

Depositor

Stephen Ekker

Article

Balciunas et al PLoS Genet. 2006 Nov 10;2(11):e169. Epub

Insert

Tol2 Transposase

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 29, 2011

Availability

Academic Institutions and Nonprofits only

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pEZT-BM

Plasmid

#74099

Purpose

Multi-purpose rapid titer bacmam expression vector

Depositor

Ryan Hibbs

Article

Morales-Perez et al Structure. 2016 Mar 24. pii: S0969-2126(

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Bacmam, baculovirus

Tags

EGFP following p10 promoter

Expression

Mammalian

Promoter

CMV, p10

Available Since

March 28, 2016

Availability

Academic Institutions and Nonprofits only

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pRH030

Plasmid

#159633

Purpose

CRISPR‐Cas9 tool for the genetic engineering of Bacillus phages

Depositor

Robert Hertel

Article

Otte et al Lett Appl Microbiol. 2020 Jul 2. doi: 10

Type

Empty backbone

Use

CRISPR; Shuttle vector

Promoter

Constitutive tracrRNA

Available Since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

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pCDNA3-GFP

Plasmid

#74165

Purpose

Mammalian expression of EGFP

Depositor

Martine Roussel

Article

Vo et al Cancer Cell. 2016 Jan 11;29(1):5-16. doi

Insert

GFP

Expression

Mammalian

Promoter

CMV

Available Since

April 11, 2016

Availability

Academic Institutions and Nonprofits only

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pLS-mP

Plasmid

#81225

Purpose

Lentivirus enhancer assay plasmid that contains a minimal promoter (mP) and a EGFP reporter gene.

Depositor

Nadav Ahituv

Article

Inoue et al Genome Res. 2016 Nov 9. pii: gr.212092.1

Type

Empty backbone

Use

Lentiviral

Promoter

Derived from pGL4.23

Available Since

Jan. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pBabe-puro largeTcDNA

Plasmid

#14088

Depositor

William Hahn

Article

Zhao et al Cancer Cell. 2003 May . 3(5):483-95.

Insert

Large T antigen (SV40gp6 simian virus)

Use

Retroviral

Expression

Mammalian

Available Since

Feb. 22, 2007

Availability

Academic Institutions and Nonprofits only

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HA-14-3-3 eta

Plasmid

#116887

Purpose

Expresses HA-tagged human 14-3-3 eta in mammalian cells

Depositor

FENG-QIAN Li, Ken-Ichi Takemaru

Article

Li et al J Cell Biol. 2008 Jun 30;181(7):1141-54.

Insert

14-3-3 eta (YWHAH Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 12, 2018

Availability

Academic Institutions and Nonprofits only

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V5-BS2-NES (Cytosol)

Plasmid

#220341

Purpose

Mammalian expression vector BS2 in cytoplasm

Depositor

Bryan Dickinson

Article

Pani et al Nat Chem. 2024 Apr 9. doi: 10.1038/s4155

Insert

BS2

Tags

NES (Nuclear exclusion sequence) and V5 tag

Expression

Mammalian

Promoter

CMV

Available Since

June 26, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
GFP-APEX2-NIK3x

Plasmid

#129274

Purpose

Expression of APEX2 in the nucleolus

Depositor

Alice Ting

Article

Fazal et al Cell. 2019 Jun 14. pii: S0092-8674(19)30

Insert

GFP-APEX2-NIK3x

Use

Lentiviral

Expression

Mammalian

Promoter

CMV promoter

Available Since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

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V5-BS2-NLS (Nucleoplasmin)

Plasmid

#220342

Purpose

Mammalian expression vector BS2 in nucleus

Depositor

Bryan Dickinson

Article

Pani et al Nat Chem. 2024 Apr 9. doi: 10.1038/s4155

Insert

BS2

Tags

NLS (Nucleus localization sequence) and V5 tag

Expression

Mammalian

Promoter

CMV

Available Since

June 18, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pAAV-CA-FLEX-dtA

Plasmid

#232236

Purpose

Expresses diphtheria toxin A subunit in Cre-positive cells.

Depositor

Naoshige Uchida

Article

Tsutsui-Kimura et al bioRxiv 2022.02.05.479267

Insert

Diphtheria toxin A subunit

Use

AAV

Expression

Mammalian

Promoter

CA

Available Since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pICH-roGFP2-synORP1

Plasmid

#191680

Purpose

Ti plasmid pICH86988 harbouring roGFP2-synORP1 for H2O2 detection in plant cells.

Depositor

Philip Mullineaux

Article

Exposito-Rodriguez et al bioRxiv 2024.01.18.576236

Insert

rredox(roGFP2-synORP1

Expression

Plant

Promoter

CaMV 35S

Available Since

March 10, 2023

Availability

Academic Institutions and Nonprofits only

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pFUW PuroR-P2A-COX8ASig-Timer

Plasmid

#239559

Purpose

Expression and mitohcondrial localization of Timer

Depositor

Tim Ahfeldt, Joel Blanchard

Article

et al iScience 28 (2025)

Insert

Timer

Use

Lentiviral

Promoter

UbC

Available Since

June 30, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pLKO.1-TSC2

Plasmid

#15478

Depositor

Do-Hyung Kim

Article

Vander Haar et al Nat Cell Biol. 2007 Mar . 9(3):316-23.

Insert

TSC2 shRNA (TSC2 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Aug. 21, 2007

Availability

Academic Institutions and Nonprofits only

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pFUW H2B-mTagBFP2-P2A-PuroR

Plasmid

#239552

Purpose

Expression and nuclear localization of mTagBFP2

Depositor

Tim Ahfeldt, Joel Blanchard

Article

et al iScience 28 (2025)

Insert

mTagBFP2

Use

Lentiviral

Promoter

UbC

Available Since

June 30, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
BK beta4

Plasmid

#113568

Purpose

mouse BK beta4 expression vector

Depositor

Robert Brenner

Article

Wang et al J Gen Physiol. 2006 Apr . 127(4):449-65.

Insert

Kcnmb4 (Kcnmb4 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pRK5 HA-Nprl2

Plasmid

#99709

Purpose

Mammalian expression of HA-Nprl2

Depositor

David Sabatini, Kuang Shen

Article

Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

Insert

Nprl2 (NPRL2 )

Tags

HA

Promoter

CMV

Available Since

Nov. 3, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pJ241-FLEX

Plasmid

#18925

Purpose

FLEX switch empty backbone

Depositor

Scott Sternson

Article

Atasoy et al J Neurosci. 2008 Jul 9. 28(28):7025-30.

Insert

FLEX switch

Use

Cre/Lox; Subcloning

Mutation

An internal Spe1 site is used for subcloning a ge…

Available Since

Jan. 29, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pFUW COX8ASig-Lemon

Plasmid

#239560

Purpose

Expression and mitochondrial localization of Lemon, a pH biosensor

Depositor

Tim Ahfeldt, Joel Blanchard

Article

et al iScience 28 (2025)

Insert

Lemon

Use

Lentiviral

Promoter

UbC

Available Since

June 30, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pFUW H2B-mEmerald-P2A-PuroR

Plasmid

#239553

Purpose

Expression and nuclear localization of mEmerald

Depositor

Tim Ahfeldt, Joel Blanchard

Article

et al iScience 28 (2025)

Insert

mEmerald

Use

Lentiviral

Promoter

UbC

Available Since

June 30, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
Mito-V5-BS2 (Mitochondrial matrix)

Plasmid

#220344

Purpose

Mammalian expression vector BS2 in mitocondria

Depositor

Bryan Dickinson

Article

Pani et al Nat Chem. 2024 Apr 9. doi: 10.1038/s4155

Insert

BS2

Tags

MTS-matrix and V5 tag

Expression

Mammalian

Promoter

CMV

Available Since

June 20, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pEGFP-C1-PRKAA1

Plasmid

#30305

Depositor

Jay Brenman

Article

Kazgan et al Mol Biol Cell. 2010 Oct 1. 21(19):3433-4

Insert

AMP-activated Protein Kinase α1 (Prkaa1 Rat)

Tags

GFP

Expression

Mammalian

Available Since

July 21, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pFUW mCherry-P2A-NeoR

Plasmid

#239570

Purpose

Cytosolic expression of mCherry

Depositor

Tim Ahfeldt, Joel Blanchard

Article

et al iScience 28 (2025)

Insert

mCherry

Use

Lentiviral

Promoter

UbC

Available Since

June 30, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pAG306-pTet07-nLuc

Plasmid

#199755

Purpose

Control construct of elongation reporter to quantify elongation of YFP

Depositor

Brian Zid

Article

Hou et al RNA. 2023 Dec;29(12):1928-1938. doi: 10.

Insert

Nanoluc

Expression

Yeast

Promoter

TetO7

Available Since

May 5, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
P2X4-pHluorin123

Plasmid

#52926

Purpose

express P2X4 protein with pHluorin at 123rd position

Depositor

Baljit Khakh

Article

Xu et al J Gen Physiol. 2014 Jul;144(1):81-104. d

Insert

mP2X4-pHluorin123

Expression

Mammalian

Available Since

June 23, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA5-AP-Ub

Plasmid

#232577

Purpose

Express Ubiquitin tagged with an Acceptor Peptide (AP)

Depositor

Sagar Bhogaraju

Article

Mukhopadhyay et al Sci Adv. 2024 Aug 9;10(32):eadp3000. doi

Insert

Ubiquitin

Tags

AviTag

Expression

Mammalian

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pRK5 HA-RagA

Plasmid

#99710

Purpose

Mammalian expression of HA-RagA

Depositor

David Sabatini, Kuang Shen

Article

Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

Insert

RagA (RRAGA )

Tags

HA

Promoter

CMV

Available Since

Nov. 3, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pmr101A-hVPS26

Plasmid

#17636

Depositor

James Hurley

Article

Shi et al Nat Struct Mol Biol. 2006 Jun . 13(6):54

Insert

VPS26 (VPS26A Human)

Tags

His

Expression

Bacterial

Available Since

April 3, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
pLSSmKate1-N1

Plasmid

#31866

Depositor

Vladislav Verkhusha

Article

Piatkevich et al Proc Natl Acad Sci U S A. 2010 Mar 23;10

Insert

LSS-mKate1

Expression

Mammalian

Mutation

K69Y/P131T/S148G/M167E/T183S/M196V compared to mK…

Promoter

CMV

Available Since

Sept. 6, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA5-AP(-3)-Ub

Plasmid

#232583

Purpose

Express Ubiquitin tagged with a modified Acceptor Peptide (AP)

Depositor

Sagar Bhogaraju

Article

Mukhopadhyay et al Sci Adv. 2024 Aug 9;10(32):eadp3000. doi

Insert

Ubiquitin

Tags

AP

Expression

Mammalian

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP-C1-Flag-SmBiT-PP1b

Plasmid

#224895

Purpose

Mammalian expression of Flag-SmBiT-PP1b

Depositor

Mathieu Bollen

Article

Claes et al Cell Chem Biol. 2023 Dec 21;30(12):1666-

Insert

Flag-SmBiT-PP1b

Expression

Mammalian

Available Since

Nov. 6, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pQCXIP-EGFP-APEX2-V5-Giantin

Plasmid

#236404

Purpose

Expresses a fluorescent Golgi-localized APEX2

Depositor

Jared Rutter

Article

Cunningham et al J Cell Sci. 2025 Mar 13:jcs.263693. doi:

Insert

EGFP-APEX2-V5-Giantin

Use

Retroviral

Tags

APEX2, EGFP, and V5

Expression

Mammalian

Available Since

April 24, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
V5-BS2-NIK3x (Nucleolus)

Plasmid

#220346

Purpose

Mammalian expression vector BS2 in nucleolus

Depositor

Bryan Dickinson

Article

Pani et al Nat Chem. 2024 Apr 9. doi: 10.1038/s4155

Insert

BS2

Tags

Nik3x (Nucleolus localization tag) and V5 tag

Expression

Mammalian

Promoter

CMV

Available Since

June 18, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
bRA66

Plasmid

#100952

Purpose

GAL1 driven Cas9. gRNA can be cloned into BplI sites.

Depositor

James Haber

Article

Anand et al Nature. 2017 Apr 20;544(7650):377-380. d

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available Since

Sept. 12, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pRK5 HA-RagC

Plasmid

#99718

Purpose

Mammalian expression of HA-RagC

Depositor

David Sabatini, Kuang Shen

Article

Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

Insert

RagC (RRAGC )

Tags

HA

Promoter

CMV

Available Since

Nov. 3, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-myc-Wnk1

Plasmid

#38779

Depositor

Melanie Cobb

Article

Xu et al J Biol Chem. 2000 Jun 2;275(22):16795-80

Insert

Wnk1 (Wnk1 Rat)

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 20, 2013

Availability

Academic Institutions and Nonprofits only
isce rfp/cfp

Plasmid

#83323

Purpose

fluorescent Isce joint substrate

Depositor

Katheryn Meek

Article

Neal et al J Immunol. 2016 Apr 1;196(7):3032-42. do

Insert

RFP +CFP joining substrate

Use

Synthetic Biology

Available Since

Jan. 17, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pENTR-D-Topo-hTRIM9

Plasmid

#51032

Purpose

entry vector for cloning human TRIM9

Depositor

Timothy Cox

Article

Short et al J Biol Chem. 2006 Mar 31;281(13):8970-80

Insert

TRIM9 (TRIM9 Human)

Use

Entry vector

Promoter

NA

Available Since

Feb. 3, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pDestTol2-QUAS:GCaMP7a

Plasmid

#184817

Purpose

Used to generate the Tg(QUAS:GCaMP7a)c594 transgenic line. GCaMP7a from Muto et al., 2013 (https://doi.org/10.1016/j.cub.2012.12.040).

Depositor

Marnie Halpern

Article

Spikol et al Elife. 2024 May 31;12:RP89516. doi: 10.7

Insert

QUAS:GCaMP7a-pA

Use

Zebrafish tol2 transgenesis

Available Since

May 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge

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