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We narrowed to 9,340 results for: puc

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Showing: 1701 - 1750 of 9340 results

  6. pUC19-U6-[BsmBI_pegRNA_entry]-tevopreQ1-term (LM1138)

    Plasmid
    #223137
    Purpose
    pU6 entry plamid for cloning prime editor tevopreQ1 epegRNAs (requires BsmBI or Esp3I digest and ligation of duplexed oligos)
    Depositor
    Benjamin Kleinstiver
    Article
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Insert
    etevopreQ1 entry plasmid backbone for epegRNA expression via human U6 promoter
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    223137-plasmid-map-sequence-id-447707

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  27. pUC57 DYSF, alt exon 1, 5a, 40a

    Plasmid
    #87889
    Purpose
    research of alternative isoforms of the protein dysferlin
    Depositor
    Jain Foundation
    Insert
    DYSF, alt exon 1, 5a, 40a (DYSF Human)
    Expression
    Bacterial
    Mutation
    alternative isoforms, see comments
    Available Since
    March 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    87889-plasmid-map-sequence-id-174216

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  30. pUC19-U6-BsmBI_cassette-CjeCas9-sgRNA (KAC482)

    Plasmid
    #133793
    Purpose
    U6 promoter sgRNA entry vector used for all CjeCas9 sgRNAs (clone spacer oligos into BsmBI cassette) - similar to V2-TGAA linker sgRNA architecture from Kim et al. Nature Communications 2017
    Depositor
    Joseph Bondy-Denomy, Benjamin Kleinstiver
    Article
    Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do
    Insert
    CjeCas9 sgRNA entry vector
    Expression
    Mammalian
    Mutation
    V2-TGAA linker sgRNA architecture from Kim et al.…
    Promoter
    U6
    Available Since
    March 30, 2020
    Availability
    Academic Institutions and Nonprofits only
    133793-plasmid-map-sequence-id-268077

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  36. pUC57 DYSF, alt exon 1a, 5a, 40a

    Plasmid
    #87897
    Purpose
    research of alternative isoforms of the protein dysferlin
    Depositor
    Jain Foundation
    Insert
    DYSF, alt exon 1a, 5a, 40a (DYSF Human)
    Expression
    Bacterial
    Mutation
    alternative isoforms, see comments
    Available Since
    March 22, 2017
    Availability
    Academic Institutions and Nonprofits only
    87897-plasmid-map-sequence-id-174248

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  38. pUC19-SARS-CoV-2-N-gene-truncated

    Plasmid
    #240635
    Purpose
    pUC19-SARS-CoV-2-N-gene-truncated plasmid is a plasmid that contains the SARS-CoV-2 N-gene and can be used as a positive control in SARS-CoV-2 research or diagnostics assays targeting the N gene.
    Depositor
    Kevin Yehl
    Insert
    SARS-CoV-2-N-gene-truncated (N )
    Use
    Synthetic Biology
    Available Since
    Sept. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    240635-plasmid-map-sequence-id-486094

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  41. pUC57-Homology Arms-BRD4-HaloTag

    Plasmid
    #228575
    Purpose
    CRISPR donor plasmid to tag human BRD4 with Halo tag; for CRISPR-mediated gene insertion
    Depositor
    Marcin Imielinski
    Insert
    Homology Arms-BRD4-HaloTag (BRD4 Human)
    Use
    CRISPR
    Expression
    Mammalian
    Available Since
    Feb. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    228575-plasmid-map-sequence-id-461799

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  46. pUC-tRNAGln-BbsI-sU6-Kan

    Plasmid
    #213016
    Purpose
    Vector for Cloning of multiple gRNAs driven by distinct promoters (tRNA-Gln and synthetic U6)
    Depositor
    Stephanie Roessler
    Insert
    tRNAGln promoter, sU6 promoter, gRNA scaffolds
    Promoter
    tRNAGln promoter
    Available Since
    Feb. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213016-plasmid-map-sequence-id-423155

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  47. pUC-ODN-hsRPL3-CterTwStrep-ΔPAM62&56

    Plasmid
    #208645
    Purpose
    Remove PAM region in HomoArms by introduce SNP rs7495557 and rs760194863, to prevent cleavage after success edit. Digest with nt.BspQI and nb.BsrDI to get ssDNA.
    Depositor
    Quanfu Ma
    Insert
    Twin Strep tag with RPL3 genome HomoArm (RPL3 Synthetic, Human)
    Use
    CRISPR; Ssdna donnor preparation for knock-in of…
    Tags
    Twin Strep
    Mutation
    Two SNP (rs749555789, rs760194863) were introduce…
    Available Since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208645-plasmid-map-sequence-id-415926

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Showing: 1701 - 1750 of 9340 results