We narrowed to 110,911 results for: %s

1. pSimpleII-NLS-NmCas9-HA-NLS(s)

   Plasmid

   #47867

   Purpose

   This plasmid encode NmCas9 with two NLS and a HA tag

   Depositor

   Erik Sontheimer, James Thomson

   Article

   Hou et al Proc Natl Acad Sci U S A. 2013 Aug 12.

   Insert

   NmCas9
   

   Use

   CRISPR

   Tags

   HA and NLS

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Sept. 25, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAGGS-SARS2-S-D614 (C-flag)

   Plasmid

   #156420

   Purpose

   Mammalian expression of SARS-CoV-2 S protein (D614) with the flag tag at C-terminus. Pseudotypes onto the MLV vector but not efficiently to VSV or lentiviral vectors.

   Depositor

   Hyeryun Choe, Michael Farzan

   Article

   Zhang et al Nat Commun. 2020 Nov 26;11(1):6013. doi:

   Insert

   SARS-CoV-2 S protein (S SARS CoV2)
   

   Tags

   Flag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. LTR5HS S. pyogenes scaffold CARGO array

   Plasmid

   #191316

   Purpose

   Contains an array of guide RNAs targeting LTR5Hs elements

   Depositor

   Joanna Wysocka

   Article

   Fuentes et al Elife. 2018 Aug 2;7. pii: 35989. doi: 10

   Insert

   Array of guide RNAs
   

   Use

   CRISPR

   Tags

   mCherry

   Expression

   Mammalian

   Available Since

   Nov. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. PL-SIN-EOS-S(4+)-EiP

   Plasmid

   #21314

   Purpose

   Lentiviral EOS reporter with Sox2 enhancer (x4), expresses EGFP and Puro resistance

   Depositor

   James Ellis

   Article

   Hotta et al Nat Methods. 2009 May . 6(5):370-6.

   Insert

   Enhanced Green Fluorescence Protein, Puromycin resistance gene
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EOS-S(4+) promoter region

   Available Since

   June 1, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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5. nontarget S. pyogenes scaffold CARGO array

   Plasmid

   #191319

   Purpose

   Contains an array of nontargeting guide RNAs

   Depositor

   Joanna Wysocka

   Article

   Fuentes et al Elife. 2018 Aug 2;7. pii: 35989. doi: 10

   Insert

   Array of guide RNAs
   

   Use

   CRISPR

   Tags

   mCherry

   Expression

   Mammalian

   Available Since

   Nov. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. AAV phSyn1(S)-FLEX-tdTomato-WPRE

   Plasmid

   #51505

   Purpose

   Can be used to generate AAV virus that will express tdTomato in the presence of Cre in neurons from the synapsin promoter

   Depositor

   Hongkui Zeng

   Article

   Oh et al Nature. 2014 Apr 10;508(7495):207-14. do

   Insert

   tdTomato
   

   Use

   AAV

   Promoter

   phSyn1

   Available Since

   May 1, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. AAV phSyn1(S)-FLEX-EGFP-WPRE

   Plasmid

   #51504

   Purpose

   Can be used to generate AAV virus that will express EGFP in the presence of Cre in neurons from the synapsin promoter

   Depositor

   Hongkui Zeng

   Article

   Oh et al Nature. 2014 Apr 10;508(7495):207-14. do

   Insert

   EGFP
   

   Use

   AAV

   Promoter

   phSyn1

   Available Since

   May 1, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1 SARS-CoV-2 S D614G

   Plasmid

   #158075

   Purpose

   Expresses SARS-CoV-2 spike protein with a D614G mutation

   Depositor

   Jeremy Luban

   Article

   Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

   Insert

   SARS-CoV-2 spike (S )
   

   Expression

   Mammalian

   Mutation

   D614G

   Available Since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1 SARS-CoV-2 S D614

   Plasmid

   #158074

   Purpose

   Expresses SARS-CoV-2 spike protein

   Depositor

   Jeremy Luban

   Article

   Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

   Insert

   SARS-CoV-2 spike (S )
   

   Expression

   Mammalian

   Available Since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. LTR5HS S. aureus scaffold CARGO array

    Plasmid

    #191317

    Purpose

    Contains an array of guide RNAs targeting LTR5Hs elements

    Depositor

    Joanna Wysocka

    Article

    Fuentes et al Elife. 2018 Aug 2;7. pii: 35989. doi: 10

    Insert

    Array of guide RNAs
    

    Use

    CRISPR

    Tags

    mCherry

    Expression

    Mammalian

    Available Since

    Nov. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCAGGS-SARS2-S-G614 (C-flag)

    Plasmid

    #156421

    Purpose

    Mammalian expression of SARS-CoV-2 S protein (G614) with the flag tag at C-terminus. Pseudotypes onto the MLV vector but not efficiently to VSV or lentiviral vectors.

    Depositor

    Hyeryun Choe, Michael Farzan

    Article

    Zhang et al Nat Commun. 2020 Nov 26;11(1):6013. doi:

    Insert

    SARS-CoV-2 S protein (S SARS CoV2)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    D614G

    Promoter

    CMV

    Available Since

    Aug. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. Skylan-S-(GGGGS)x3-β-Actin

    Plasmid

    #128938

    Purpose

    fluorescnece reporter of β-Actin with Skylan-S. Express in mammalian cells

    Depositor

    Shimon Weiss

    Article

    Yi et al Biomed Opt Express. 2019 Apr 16;10(5):24

    Insert

    Skylan-S
    

    Tags

    β-actin

    Expression

    Mammalian

    Available Since

    Nov. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pαH-S-GSAS-OMICRON.BA.2-RBD

    Plasmid

    #184830

    Purpose

    Mammalian cell expression of RBD of SARS-CoV-2 spike protein of variant OMICRON.BA.2 with furin side replaced by GSAS and with 2 Proline substitution at 986 and 987

    Depositor

    Priyamvada Acharya

    Article

    Stalls et al Cell Rep. 2022 Jun 28;39(13):111009. doi

    Insert

    S-GSAS-OMICRON.BA2-RBD (S Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    6X His tag

    Expression

    Mammalian

    Mutation

    SARS-CoV-2 Spike RBD protein of OMICRON.BA.2 vari…

    Available Since

    June 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLN382 (FuGW-S(HIF1A)p-mKate2 )

    Plasmid

    #105180

    Purpose

    synthetic promoter HIF1A drives mKate2 expression

    Depositor

    Timothy Lu

    Article

    Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

    Insert

    S(HIF1A)p-mKate2
    

    Use

    Lentiviral and Synthetic Biology

    Expression

    Mammalian

    Available Since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRK5 FLAG DEPTOR (13×S/T->A)

    Plasmid

    #21702

    Depositor

    David Sabatini

    Article

    Peterson et al Cell. 2009 May 29;137(5):873-86. doi: 10

    Insert

    DEPTOR 13xA (DEPTOR Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Nonphosphorylatable mutant. 13xA. Also has N204S …

    Available Since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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16. pαH-S-GSAS-OMICRON.BA.1-RBD

    Plasmid

    #184831

    Purpose

    Mammalian cell expression of RBD of SARS-CoV-2 spike protein of variant OMICRON.BA.1 with furin side replaced by GSAS and with 2 Proline substitution at 986 and 987

    Depositor

    Priyamvada Acharya

    Article

    Stalls et al Cell Rep. 2022 Jun 28;39(13):111009. doi

    Insert

    S-GSAS-OMICRON.BA.1 (S Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    6X His tag

    Expression

    Mammalian

    Mutation

    SARS-CoV-2 Spike RBD protein of OMICRON.BA.1 vari…

    Available Since

    June 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCAGGS-SARS2-S-D614 (NC-flag)

    Plasmid

    #156418

    Purpose

    Mammalian expression of SARS-CoV-2 S protein (D614) with the flag tag at N- and C-termini. Pseudotypes onto the MLV vector but not efficiently to VSV or lentiviral vectors.

    Depositor

    Hyeryun Choe, Michael Farzan

    Article

    Zhang et al Nat Commun. 2020 Nov 26;11(1):6013. doi:

    Insert

    SARS-CoV-2 S protein (S SARS CoV2)
    

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. G protein alpha-s(alpha3beta5/G226A)

    Plasmid

    #55798

    Purpose

    In addition to the mutations in alpha-s(alpha3beta5), this dominant negative G protein alpha-s mutant contains the G226A mutation, which impairs activating conformation changes in Switch II.

    Depositor

    Catherine Berlot

    Article

    Berlot et al J Biol Chem. 2002 Jun 7;277(23):21080-5.

    Insert

    alpha-s (alpha3beta5/G226A) (Gnas Rat)
    

    Tags

    internal EE epitope (residues 189-194 in alpha-s …

    Expression

    Mammalian

    Mutation

    N271K, K274D, R280K, T284D, I285T, G226A in alpha…

    Promoter

    CMV

    Available Since

    Aug. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLN592 (FuGW-S(USF1)p-mKate2)

    Plasmid

    #105204

    Purpose

    synthetic promoter USF1 drives mKate2 expression

    Depositor

    Timothy Lu

    Article

    Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

    Insert

    S(USF1)p-mKate2
    

    Use

    Lentiviral and Synthetic Biology

    Expression

    Mammalian

    Available Since

    Aug. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLVX-SV40-Galpha-s ONE-GO

    Plasmid

    #204327

    Purpose

    GPCR/G protein BRET biosensor

    Depositor

    Mikel Garcia-Marcos

    Article

    Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

    Insert

    SV40 Galpha-s ONE-GO (GNAS Human)
    

    Use

    Lentiviral

    Available Since

    Jan. 19, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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