We narrowed to 6,236 results for: nls

1. pME-nlsGFP-P2A

   Plasmid

   #80808

   Purpose

   nuclear localization signal fused to N-terminus of GFP (nlsGFP) without stop codon, followed by P2A

   Depositor

   Philip Washbourne

   Article

   Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

   Insert

   minimal cytomegalovirus immediate early enhancer/promoter
   

   Available Since

   Nov. 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pMEH275 NLS dTomato

   Plasmid

   #168210

   Purpose

   Mammalian expression of nuclear-localized dTomato

   Depositor

   Joshua Leonard

   Article

   Hung et al J Extracell Vesicles. 2016 May 13;5:3102

   Insert

   NLS dTomato
   

   Use

   Lentiviral

   Tags

   NLS

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. NLS-4LNDDE488Q-pCDNA3.1(+)

   Plasmid

   #172314

   Purpose

   Expresses ADAR2 Deaminase Domain (DD) hyperactive mutant E488Q fused with 4 repeats of lambda N peptide (4LN) with a Nuclear Localization Signal (NLS)

   Depositor

   Joshua Rosenthal

   Article

   Vallecillo-Viejo et al RNA Biol. 2018 Jan 2;15(1):104-114. doi:

   Insert

   Lambda N peptide - human ADAR2 Deaminase Domain E488Q
   

   Tags

   6xHis, Flag, and Nuclear Localization Signal of S…

   Expression

   Mammalian

   Mutation

   changed the Glutamic acid 488 in the Deaminase Do…

   Promoter

   CMV

   Available Since

   Aug. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCMV-BE3-FNLS(RA)

   Plasmid

   #112671

   Purpose

   CMV expression vector for BE3-FNLS construct (codon optimized)

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   BE3-FNLS
   

   Tags

   3x FLAG

   Expression

   Mammalian

   Mutation

   NLS sequence at the N-terminus and D10A

   Promoter

   CMV

   Available Since

   July 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pQC NLS mPlum IX

   Plasmid

   #37356

   Depositor

   Connie Cepko

   Article

   Beier et al Dev Biol. 2011 May 15;353(2):309-20. Epu

   Insert

   NLS mPlum
   

   Available Since

   July 30, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. NLS-LucDM-GFP

   Plasmid

   #127796

   Purpose

   The expressed ORF encodes nucleus location signal with GFP and destabilized mutant Luciferase.

   Depositor

   Xiaolu Yang

   Article

   Guo et al J Vis Exp. 2016 Aug 28;(114). doi: 10.37

   Insert

   destabilized mutant (DM) Luciferase
   

   Tags

   EGFP and FLAG-NLS

   Expression

   Mammalian

   Mutation

   R188Q, R261Q in Firefly Luciferase insert

   Promoter

   CMV

   Available Since

   July 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pTrex-b-NLS-hSpCas9

   Plasmid

   #62543

   Purpose

   Expression of Cas9 in T. cruzi

   Depositor

   Rick Tarleton

   Article

   Peng et al MBio. 2014 Dec 30;6(1). pii: e02097-14.

   Insert

   NLS-hSpCas9
   

   Use

   CRISPR; Trypanosoma cruzi expression

   Tags

   3xFLAG and NLS

   Promoter

   Ribo-HX1

   Available Since

   April 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pEF1a-2xSV40_NLS-NLuc

   Plasmid

   #135953

   Purpose

   Expresses the NanoLuciferase targeted to the nucleus in mammalian cells.

   Depositor

   Antonio Amelio

   Article

   Parag-Sharma et al ACS Synth Biol. 2019 Dec 13. doi: 10.102

   Insert

   NanoLuciferase
   

   Tags

   2xSV40-NLS

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAd-PV-NLS-CD-DsRed

   Plasmid

   #16345

   Depositor

   Anton Bennett

   Article

   Rodrigues et al J Biol Chem. 2007 Jun 8. 282(23):17061-8

   Insert

   PV-DsRed-NLS-CD (Pvalb Rat)
   

   Use

   Adenoviral

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Functional Ca2+binding site CD was inactivated by…

   Available Since

   Feb. 26, 2008

   Availability

   Academic Institutions and Nonprofits only

10. mCherry-PCNA-19-NLS-4

    Plasmid

    #55116

    Purpose

    Localization: Nucleus, Excitation: 587, Emission: 610

    Depositor

    Michael Davidson

    Insert

    NM_002592.2 (PCNA Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

11. GFP-NPM NLS1D

    Plasmid

    #13284

    Depositor

    Xin Wang

    Article

    Wang et al Nat Cell Biol. 2005 Aug . 7(8):823-30.

    Insert

    NPM (NPM1 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Deletion of aa134-aa142

    Available Since

    Oct. 16, 2006

    Availability

    Academic Institutions and Nonprofits only

12. GFP-PKI nls

    Plasmid

    #118301

    Purpose

    Expression of PKIa

    Depositor

    Philip Stork

    Article

    Billiard et al J Biol Chem. 2001 Aug 17;276(33):31238-4

    Insert

    PKI nls (aa 2-36)
    

    Tags

    Flag and GFP

    Expression

    Mammalian

    Mutation

    inserting 2xnls

    Available Since

    Nov. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA5-TO-PV-NLS-Dsred

    Plasmid

    #16341

    Depositor

    Anton Bennett

    Article

    Rodrigues et al J Biol Chem. 2007 Jun 8. 282(23):17061-8

    Insert

    PV-DsRed-NLS (Pvalb Rat)
    

    Tags

    Myc and NLS

    Expression

    Mammalian

    Available Since

    March 12, 2008

    Availability

    Academic Institutions and Nonprofits only

14. GFP-NPM NLS1/2D

    Plasmid

    #13287

    Depositor

    Xin Wang

    Article

    Wang et al Nat Cell Biol. 2005 Aug . 7(8):823-30.

    Insert

    NPM (NPM1 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Deletion of aa134-aa142, aa150-aa155

    Available Since

    Oct. 16, 2006

    Availability

    Academic Institutions and Nonprofits only

15. pLBS.CAG/NLS-mScarlet

    Plasmid

    #129336

    Purpose

    nucleus marker, lentivirus

    Depositor

    Yasushi Okada

    Insert

    mScarlet (Addgene 85042)
    

    Use

    Lentiviral

    Available Since

    Oct. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

16. mRuby-PCNA-19-NLS-4

    Plasmid

    #55876

    Purpose

    Localization: Nucleus, Excitation: 558, Emission: 605

    Depositor

    Michael Davidson

    Insert

    PCNA-19 NLS (PCNA Human)
    

    Tags

    3xNLS and mRuby

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 12, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

17. pcDNA3.1(+)-sapphireAKAR-NLS

    Plasmid

    #118467

    Purpose

    cp-T-sapphire-based single-color PKA biosensor targeted to the nucleus.

    Depositor

    Jin Zhang

    Article

    Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

    Insert

    sapphireAKAR-NLS
    

    Tags

    Nuclear localization signal (NLS)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pHeCAHS-mEGFP-NLS

    Plasmid

    #205014

    Purpose

    The vector contains 1kbp of the upstream and downstream regions of the chas gene (BV898_02951, CAHS 86272) from Hypsibius exemplaris, with the mEGFP gene with NLS sequence positioned in between.

    Depositor

    Kazuharu Arakawa

    Article

    Tanaka et al Proc Natl Acad Sci U S A. 2023 Jan 31;12

    Insert

    mEGFP-NLS flanked by 1kbp upstream and downstream of the chas gene from H. exemplaris
    

    Use

    Tardigrade expression

    Available Since

    Aug. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Construct 33 - NFKBRp_Cas9_3xNLS_p2a-puroR

    Plasmid

    #81255

    Purpose

    Lentiviral construct for building stable cell lines expressing Cas9 in the presence of TNFa via puromycin selection

    Depositor

    Timothy Lu

    Article

    Perli et al Science. 2016 Aug 18. pii: aag0511.

    Insert

    Cas9-3xNLS
    

    Use

    Lentiviral and Synthetic Biology

    Expression

    Mammalian

    Available Since

    Sept. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Flag-nls-NgAgo-GK

    Plasmid

    #86435

    Purpose

    Expresses a Flag tag on the N-terminal

    Depositor

    Gaétan Burgio

    Article

    Khin et al PLoS One. 2017 Jun 13;12(6):e0178768. do

    Insert

    NgAgo
    

    Tags

    Flag tag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge