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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 9,217 results for: sgRNA

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Showing: 681 - 700 of 9217 results

  3. lenti-EGFR-L858R-T790M-dual-nick sgRNA

    Plasmid
    #214101
    Purpose
    Lentiviral vector expressing nicking sgRNAs for the induction of EGFR L858R and T790M mutations, through tandem U6 expression of the two sgRNAs using independent U6 promoters.
    Depositor
    Ting Zhou
    Publication
    Wu et al Nat Commun. 2024 Dec 30;15(1):10824. doi
    Insert
    EGFR L858R nicking sgRNA/EGFR T790M nicking sgRNA
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    hU6
    Available since
    June 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    214101-plasmid-map-sequence-id-429357

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  12. pIA33

    Plasmid
    #120803
    Purpose
    E. coli-C. difficile shuttle vector for CRISPR interference (CRISPRi) in C. difficile; sgRNA targets rfp; Pxyl::dCas9-opt Pgdh::sgRNA-rfp
    Depositor
    Craig Ellermeier, David S. Weiss
    Publication
    Muh et al J Bacteriol. 2019 Feb 11. pii: JB.00711-
    Inserts
    deactivated nuclease dCas9, codon-optimized for C. difficile
    single guide RNA targeting red fluorescent protein
    Use
    CRISPR
    Expression
    Bacterial
    Mutation
    Base-pairing region targets red fluorescent prote…
    Promoter
    Pgdh and Pxyl
    Available since
    Feb. 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    120803-plasmid-map-sequence-id-235316

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  15. phU6 ST

    Plasmid
    #188712
    Purpose
    sgRNA plasmid encoding hU6 promoter driving an anti-safe targeting sgRNA
    Depositor
    David Tobin
    Publication
    Brewer et al Cell Rep. 2022 Dec 13;41(11):111817. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Safe Targeting sgRNA
    Use
    CRISPR and Synthetic Biology
    Available since
    Dec. 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    188712-plasmid-map-sequence-id-370671

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  16. pmU6 ST

    Plasmid
    #188713
    Purpose
    sgRNA plasmid encoding mU6 promoter driving an anti-safe targeting sgRNA
    Depositor
    David Tobin
    Publication
    Brewer et al Cell Rep. 2022 Dec 13;41(11):111817. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Safe Targeting sgRNA
    Use
    CRISPR and Synthetic Biology
    Available since
    Dec. 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    188713-plasmid-map-sequence-id-370668

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  17. phH1 ST

    Plasmid
    #188715
    Purpose
    sgRNA plasmid encoding hH1 promoter driving an anti-safe targeting sgRNA
    Depositor
    David Tobin
    Publication
    Brewer et al Cell Rep. 2022 Dec 13;41(11):111817. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Safe Targeting sgRNA
    Use
    CRISPR and Synthetic Biology
    Available since
    Dec. 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    188715-plasmid-map-sequence-id-370674

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  20. pMZ377

    Plasmid
    #74214
    Purpose
    Combined sgRNA/Cas9 vector with LEU2 marker
    Depositor
    Mikel Zaratiegui
    Inserts
    Cas9
    rrk1:sgRNA
    Use
    CRISPR
    Expression
    Yeast
    Mutation
    Silent muation of the CspCI site
    Promoter
    adh1 and rrk1
    Available since
    March 31, 2016
    Availability
    Academic Institutions and Nonprofits only
    74214-plasmid-map-sequence-id-141045

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Showing: 681 - 700 of 9217 results