We narrowed to 8,785 results for: sgrna

1. pMA-MsgRNA-EGFP

   Plasmid

   #80794

   Purpose

   For insertion of gRNA array containing 11-30 gRNA modules

   Depositor

   Yonglun Luo

   Article

   Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 30, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEF005-sgRNA-shuffle-in

   Plasmid

   #104441

   Purpose

   Provide and shuffle a cassette of AtU6:sgRNA-transRNA into SM-destination vectors (pRW006 and pRW004) with golden gate cloning strategy. Work together with pEF004.

   Depositor

   Detlef Weigel

   Article

   Wu et al Plant Methods. 2018 Aug 2;14:65. doi: 10

   Insert

   sgRNA-transRNA transcription by U6 promoter
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial and Plant

   Mutation

   Promoter

   Available Since

   Jan. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEF004-sgRNA-shuffle-in

   Plasmid

   #104440

   Purpose

   Provide and shuffle a cassette of AtU6:sgRNA-transRNA into SM-destination vectors (pRW006 and pRW004) with golden gate cloning strategy. Work together with pEF005

   Depositor

   Detlef Weigel

   Article

   Wu et al Plant Methods. 2018 Aug 2;14:65. doi: 10

   Insert

   sgRNA-transRNA transcription by U6 promoter
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial and Plant

   Mutation

   Promoter

   U6 promoter

   Available Since

   Dec. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. px330-UFM1 sgRNA2

   Plasmid

   #134634

   Purpose

   contains sgRNA targeting human UFM1 for gene knockout

   Depositor

   Yihong Ye

   Article

   Wang et al Cell Res. 2020 Jan;30(1):5-20. doi: 10.1

   Insert

   UFM1 sgRNA2 (UFM1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. px330-UFM1 sgRNA1

   Plasmid

   #134633

   Purpose

   contains sgRNA targeting human UFM1 for gene knockout

   Depositor

   Yihong Ye

   Article

   Wang et al Cell Res. 2020 Jan;30(1):5-20. doi: 10.1

   Insert

   UFM1 sgRNA1 (UFM1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pX330_Actb sgRNA / hSpCas9

   Plasmid

   #172832

   Purpose

   Mammalian expression of a sgRNA targeting the intron 1of Actb (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.

   Depositor

   Haining Zhong

   Article

   Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

   Insert

   sgRNA targeting intron 1 of Actb under the U6 promotor and hSpCas9 under the CAG promotor
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. px335 Mettl14 sgRNA #2

   Plasmid

   #61514

   Purpose

   encodes sgRNA sequence for targeting mouse Mettl14 locus (Cas9-Nickase strategy)

   Depositor

   Jacob Hanna

   Article

   Geula et al Science. 2015 Feb 27;347(6225):1002-6. d

   Insert

   gccgctcccggatctcctgc
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Jan. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

8. pCJH009_proD_Cas12c-sgRNA-GFP_CAM

   Plasmid

   #183070

   Purpose

   Plasmid expressing Cas12c sgRNA targeting GFP (1) for in vivo interference assay in bacteria.

   Depositor

   Jennifer Doudna

   Article

   Huang et al Mol Cell. 2022 Jun 2;82(11):2148-2160.e4

   Insert

   Cas12c single guide RNA targeting GFP
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   June 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pX459-HypaCas9-ANLN_sgRNA

   Plasmid

   #183874

   Purpose

   pX459V2.0-HypaCas9 plasmid with ANLN sgRNA for N-terminal tagging of anillin in human cells.

   Depositor

   Alisa Piekny

   Article

   Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

   Insert

   ANLN sgRNA spacer (ANLN Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   May 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. AAV_Actb HMEJ donor_U6_sgRNA_EF1a_GFP_polyA

    Plasmid

    #97308

    Purpose

    HMEJ donor for fusing a p2A-mCherry reporter to mouse Actb. EGFP driven by EF1a promoter and U6-driven sgRNAs targeting Actb. AAV backbone.

    Depositor

    Hui Yang

    Article

    Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

    Insert

    Actb HMEJ donor
    

    Use

    AAV and Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6, EF1a

    Available Since

    Aug. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. px330-UFL1 sgRNA2

    Plasmid

    #134636

    Purpose

    contains sgRNA targeting human UFL1 for gene knockout

    Depositor

    Yihong Ye

    Article

    Wang et al Cell Res. 2020 Jan;30(1):5-20. doi: 10.1

    Insert

    UFL1 sgRNA2 (UFL1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pX459-HypaCas9-ECT2_sgRNA

    Plasmid

    #183873

    Purpose

    pX459V2.0-HypaCas9 plasmid with ECT2 sgRNA for N-terminal tagging of Ect2 in human cells.

    Depositor

    Alisa Piekny

    Article

    Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

    Insert

    ECT2 sgRNA spacer (ECT2 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Dec. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pX330_ACTB-i4 sgRNA / hSpCas9

    Plasmid

    #172828

    Purpose

    Mammalian expression of a sgRNA targeting the intron 1 position 4 of ACTB (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.

    Depositor

    Haining Zhong

    Article

    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

    Insert

    sgRNA targeting the intron 1 of ACTB under the U6 promotor and hSpCas9 under the CAG promotor
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLentiCriprV2-sgRNA-PER1-#1

    Plasmid

    #189987

    Purpose

    Lentiviral Cas9/sgRNA vector targeting C-terminus of hPER1, works with Addgene 189979-189982

    Depositor

    Achim Kramer

    Article

    Gabriel et al Proc Natl Acad Sci U S A. 2024 Aug 20;12

    Insert

    Period1 (PER1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. lentiCRISPR - EGFP sgRNA 6

    Plasmid

    #51765

    Purpose

    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an EGFP targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.

    Depositor

    Feng Zhang

    Article

    Shalem et al Science. 2014 Jan 3;343(6166):84-7. doi:

    Inserts

    Cas9
    Puromycin resistance
    EGFP sgRNA 6
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EFS and U6

    Available Since

    March 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. px330-UFL1 sgRNA1

    Plasmid

    #134635

    Purpose

    contains sgRNA targeting human UFL1 for gene knockout

    Depositor

    Yihong Ye

    Article

    Wang et al Cell Res. 2020 Jan;30(1):5-20. doi: 10.1

    Insert

    UFL1 sgRNA1 (UFL1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. sgRNA3_GAL4UAS-Luciferase reporter

    Plasmid

    #64159

    Purpose

    Photoactivatable transcription system. Lentiviral expression of sgRNA3 to target GAL4UAS-luciferase. Also contains a CMV-puro-t2A-mCherry expression cassette

    Depositor

    Moritoshi Sato

    Article

    Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

    Insert

    sgRNA3 for GAL4UAS-Luciferase reporter
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    April 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. ATM N-terminal sgRNA

    Plasmid

    #207089

    Purpose

    pX330 based plasmid for expression of Cas9 and the ATCATTAAGTACTAGACTCA sgRNA to target the ATM locus.

    Depositor

    Jens Schmidt

    Article

    Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

    Insert

    ATCATTAAGTACTAGACTCA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and U6

    Available Since

    April 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. AAV_Actb MMEJ donor_U6_sgRNA_EF1a_GFP_polyA

    Plasmid

    #97311

    Purpose

    MMEJ donor for fusing a p2A-mCherry reporter to mouse Actb. EGFP driven by EF1a promoter and U6-driven sgRNAs targeting Actb. AAV backbone.

    Depositor

    Hui Yang

    Article

    Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

    Insert

    Actb MMEJ donor
    

    Use

    AAV and Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6, EF1a

    Available Since

    July 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pX335 HTT sgRNA-a

    Plasmid

    #87201

    Purpose

    pX335 vector encoding SpCas9n and a chimeric guide RNA targeting exon 1 of HTT

    Depositor

    Mahmoud Pouladi

    Article

    Xu et al Stem Cell Reports. 2017 Feb 21. pii: S22

    Insert

    HTT (HTT Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    July 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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