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pET32a-hNRG1a

Plasmid

#199235

Purpose

Bacterial production of soluble, active target proteins; Nterm thrombin and enterokinase cleavage sites.

Depositor

Nilson Zanchin

Article

Ferreira et al Mol Biol Rep. 2022 Dec;49(12):11715-1172

Insert

Neuregulin (NRG1 Human)

Tags

TrxA-6xHis-S-tag

Expression

Bacterial

Promoter

T7

Available Since

May 10, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmNanos-del50-236_AC

Plasmid

#148459

Purpose

Insect Expression of DmNanos-del50-236

Depositor

Elisa Izaurralde

Article

Raisch et al EMBO J. 2016 May 2;35(9):974-90. doi: 10

Insert

DmNanos-del50-236 (nos Fly)

Expression

Insect

Mutation

sequence contains a deletion of N283 and a mutati…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pGTVL2-BIRC2-BIR3

Plasmid

#196367

Purpose

protein expression of the GST-tagged BIR3 domain

Depositor

Stefan Knapp

Article

Schwalm et al Front Cell Dev Biol. 2022 May 26;10:8865

Insert

BIRC2 BIR3 domain

Tags

His6-GST-TEV

Expression

Bacterial

Mutation

contains only S260-Y352

Promoter

T7

Available Since

Feb. 28, 2023

Availability

Academic Institutions and Nonprofits only

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YW347_Ptbb2UTR-loxP-RFP-miniMOS

Plasmid

#196064

Purpose

a plasmid modified from miniMOS vector pCFJ910. the modification including insertions of myo2p::TagRFP downstream to NeoR CDS and 2 loxP sites flanking the NeoR and tagRFP cassettes.

Depositor

Wenxing Yang

Article

Li et al PeerJ. 2023 May 17;11:e15433. doi: 10.77

Inserts

myo2p driven tagRFP
NeoR
Minimal Mos1

Expression

Worm

Promoter

myo-2

Available Since

Jan. 31, 2023

Availability

Academic Institutions and Nonprofits only

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deltaVP1d63C + VP2C-GFPdeg

Plasmid

#182481

Purpose

YIp for expressing Murine polyomavirus deltaVP1 with the 63 C-ter residues truncated (GAL1 promoter) and destabilised GFP tagged with VP2C (GAL10 promoter). Contains uracil marker (K. lactis URA3).

Depositor

Claudia Vickers

Article

Cheah et al bioRxiv 2022.11.24.517869

Inserts

VP2C-GFPdeg
deltaVP1d63C

Use

Synthetic Biology; Metabolic engineering

Expression

Yeast

Promoter

GAL1 and GAL10

Available Since

Dec. 6, 2022

Availability

Academic Institutions and Nonprofits only

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Linked VLP NES

Plasmid

#182432

Purpose

Yeast integrative plasmid for expressing Murine polyomavirus deltaVP1 (GAL1 promoter) and fusion protein VP2C-ERG20-AcNES1 (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).

Depositor

Claudia Vickers

Article

Cheah et al bioRxiv 2022.11.24.517869

Inserts

VP2C-FPPS-NES
deltaVP1

Use

Synthetic Biology; Metabolic engineering

Expression

Yeast

Promoter

GAL1 and GAL10

Available Since

Dec. 6, 2022

Availability

Academic Institutions and Nonprofits only

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Linked VLP NES (M)

Plasmid

#182434

Purpose

YIp for expressing Murine polyomavirus deltaVP1 (GAL1 promoter) and fusion protein VP2C-ERG20(F96W-N127W)-AcNES1 (GAL10 promoter). Contains uracil marker (K. lactis URA3).

Depositor

Claudia Vickers

Article

Cheah et al bioRxiv 2022.11.24.517869

Inserts

VP2C-FPPS(M)-NES
deltaVP1

Use

Synthetic Biology; Metabolic engineering

Expression

Yeast

Promoter

GAL1 and GAL10

Available Since

Dec. 6, 2022

Availability

Academic Institutions and Nonprofits only

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Linked VLP NES (G)

Plasmid

#182476

Purpose

YIp for expressing Murine polyomavirus deltaVP1 (GAL1 promoter) and fusion protein VP2C-ERG20(F96C)-AcNES1 (GAL10 promoter). Contains uracil marker (K. lactis URA3).

Depositor

Claudia Vickers

Article

Cheah et al bioRxiv 2022.11.24.517869

Inserts

VP2C-FPPS(G)-NES
deltaVP1

Use

Synthetic Biology; Metabolic engineering

Expression

Yeast

Promoter

GAL1 and GAL10

Available Since

Dec. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1/V5-HisA-RvCAHS8-GS-AcGFP1

Plasmid

#192470

Purpose

Transient expression of RvCAHS8 (fly codon optimized)-GS_linker-AcGFP1 in Drosophila cells

Depositor

Takekazu Kunieda

Article

Tanaka et al PLoS Biol. 2022 Sep 6;20(9):e3001780. do

Insert

CAHS8

Tags

AcGFP1

Expression

Insect

Promoter

Ac5 promoter

Available Since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1/V5-HisA-RvCAHS3-GS-AcGFP1

Plasmid

#192469

Purpose

Transient expression of RvCAHS3 (fly codon optimized)-GS_linker-AcGFP1 in Drosophila cells

Depositor

Takekazu Kunieda

Article

Tanaka et al PLoS Biol. 2022 Sep 6;20(9):e3001780. do

Insert

CAHS3

Tags

AcGFP1

Expression

Insect

Promoter

Ac5 promoter

Available Since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pEE044

Plasmid

#176801

Purpose

Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression

Depositor

Gregg Beckham

Article

Erickson et al Nat Commun. 2022 Dec 21;13(1):7850. doi:

Insert

413

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-1-DGCR8-2xdsRBD(+6)

Plasmid

#171542

Purpose

Encodes a DGCR8 fragment extending from E502 to the C-terminus

Depositor

Yan Zeng

Article

Zhang et al Acta Biochim Biophys Sin (Shanghai). 202

Insert

DGCR8 (DGCR8 Human)

Expression

Bacterial

Mutation

a six amino acid_TRLTEG_insertion in the L1 region

Available Since

Aug. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pSUMO FAPP2-GLTPH

Plasmid

#170739

Purpose

The plasmids contain inserts (ORFs) for expressing various members of the GlycoLipid Transfer Protein (GLTP) superfamily.

Depositor

Rhoderick Brown

Article

Ochoa-Lizarralde et al J Biol Chem. 2018 Oct 26;293(43):16709-1

Insert

PLEKHA8-GLTPH (PLEKHA8 Human)

Tags

SUMO

Expression

Bacterial

Available Since

July 8, 2021

Availability

Academic Institutions and Nonprofits only

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VP2C-GFP-MIOX only

Plasmid

#166684

Purpose

Yeast integrative plasmid for expressing mouse myo-inositol oxygenase fused to VP2C-GFP (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).

Depositor

Claudia Vickers

Article

Cheah et al ACS Synth Biol. 2021 Dec 17;10(12):3251-

Insert

VP2C-GFP-MIOX

Use

Synthetic Biology

Expression

Yeast

Promoter

GAL10

Available Since

April 9, 2021

Availability

Academic Institutions and Nonprofits only

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deltaVP1 only

Plasmid

#166675

Purpose

Yeast integrative plasmid for expressing deltaVP1, an NLS deletion mutant of Murine polyomavirus VP1 (GAL1 promoter). Contains uracil auxotrophic marker (K. lactis URA3).

Depositor

Claudia Vickers

Article

Cheah et al ACS Synth Biol. 2021 Dec 17;10(12):3251-

Insert

Murine polyomavirus deltaVP1 (NLS deletion mutant)

Use

Synthetic Biology

Expression

Yeast

Promoter

GAL1

Available Since

April 8, 2021

Availability

Academic Institutions and Nonprofits only

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pCFJ2560 - pEXP(mosTI, unc-119)[Peft-3 | ceGFP(PATC, NLS) | tbb-2 3'UTR]

Plasmid

#159825

Purpose

Single copy or array insertion by MosTI using split unc-119 selection

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Insert

pEXP(mosTI, unc-119)[Peft-3 | ceGFP(PATC, NLS) | tbb-2 3'UTR]

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pRS314-U6-A34G/A35G/G96C/A97C/U100C/U101C

Plasmid

#81239

Purpose

U6 telestem mutant (A34G/A35G/G96C/A97C/U100C/U101C) for in vivo studies

Depositor

Aaron Hoskins

Article

Rodgers et al Nucleic Acids Res. 2016 Dec 15;44(22):10

Insert

U6 RNA with A34G/A35G/G96C/A97C/U100C/U101C mutation (snR6 Budding Yeast)

Expression

Yeast

Mutation

U6 telestem mutant (A34G/A35G/G96C/A97C/U100C/U10…

Available Since

Sept. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-AcrIIA1(CTD)-NLS(SV40) (KAC438)

Plasmid

#133796

Purpose

CMV and T7 promoter expression plasmid for human codon optimized AcrIIA1 (truncated C-terminal domain only) with C-terminal NLS (SV40)

Depositor

Joseph Bondy-Denomy, Benjamin Kleinstiver

Article

Osuna et al Cell Host Microbe. 2020 Apr 18. pii: S19

Insert

human codon optimized AcrIIA1 (truncated C-terminal domain only)

Tags

NLS(SV40)

Expression

Mammalian

Mutation

C-terminal domain only

Promoter

CMV and T7

Available Since

April 27, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-AcrIIA1(T114A/F115A)-NLS(SV40) (KAC688)

Plasmid

#133798

Purpose

CMV and T7 promoter expression plasmid for human codon optimized AcrIIA1(T114A/F115A) with C-terminal NLS (SV40)

Depositor

Joseph Bondy-Denomy, Benjamin Kleinstiver

Article

Osuna et al Cell Host Microbe. 2020 Apr 18. pii: S19

Insert

human codon optimized AcrIIA1(T114A/F115A)

Tags

NLS(SV40)

Expression

Mammalian

Mutation

T114A/F115A

Promoter

CMV and T7

Available Since

April 27, 2020

Availability

Academic Institutions and Nonprofits only

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pDEST40-2XFL-Src-S4-UD

Plasmid

#140305

Purpose

Expresses Flag-tagged human Src-S4-UD in mammalian cells for structure-function studies

Depositor

Mark Moasser

Article

Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

Insert

Src deletion mutant (SRC Human)

Tags

2X Flag

Expression

Mammalian

Mutation

contains only the SH4 and UD regions

Promoter

CMV

Available Since

April 15, 2020

Availability

Academic Institutions and Nonprofits only

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