We narrowed to 166,390 results for: addgene

1. pLX302 MX1-V5 puro

   Plasmid

   #158640

   Purpose

   Lentiviral expression vector for constitutive expression of V5-tagged MX1.

   Depositor

   Kevin Janes

   Article

   Lopacinski et al Cell Syst. 2021 Apr 21;12(4):304-323.e13

   Insert

   Mx1 (MX1 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Available Since

   Oct. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pWZL hygro H-Ras V12

   Plasmid

   #18749

   Depositor

   Scott Lowe

   Article

   Serrano et al Cell. 1997 Mar 7. 88(5):593-602.

   Insert

   H-Ras V12 (HRAS Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Mutation

   V12

   Available Since

   July 3, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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3. GRIK1_pCSdest

   Plasmid

   #53832

   Depositor

   Roger Reeves

   Article

   Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

   Insert

   GRIK1 (GRIK1 Human)
   

   Expression

   Mammalian

   Promoter

   SP6 and CMV

   Available Since

   March 27, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

4. pCAG-mCherry-FKBP-GEF(TIAM1)

   Plasmid

   #85156

   Purpose

   acute activation of Rac through rapamycin-induced translocation to membrane-localized FRB

   Depositor

   Tobias Meyer

   Article

   Hayer et al Nat Cell Biol. 2016 Dec;18(12):1311-1323

   Insert

   Fusion of mCherry, FKBP, and the DH-domain of human TIAM1 (amino acids 1012-1250)
   

   Tags

   mCherry-FKBP

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Feb. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. AAV-mPGK-eGFP (beta-globin intron)

   Plasmid

   #162513

   Purpose

   AAV vector plasmid expressing enhanced green fluorescent protein (eGFP) under the mouse phosphoglycerate kinase (mPGK) promoter that is upstream of a beta-globin intron

   Depositor

   Bart Nieuwenhuis

   Article

   Nieuwenhuis et al Gene Ther. 2020 Jun 23. pii: 10.1038/s41

   Insert

   eGFP
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   mPGK

   Available Since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFB-U6-sgNeuN-gRNA-hSyn-mCherry-WPRE-SV40pA

   Plasmid

   #128346

   Purpose

   pAAV encoding control gRNA for CRISPR gRNAs listed above

   Depositor

   Ryan Drenan

   Article

   Peng et al Eur J Neurosci. 2018 May 19. doi: 10.111

   Insert

   Rbfox3 (NeuN) (Rbfox3 Mouse)
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   July 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pX330-PITCh-H3C2

   Plasmid

   #207780

   Purpose

   Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of H3C2 for knock-in.

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   sgRNA Targeting C-terminus of H3C2 (H3C2 Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Dec. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBA409

   Plasmid

   #85971

   Purpose

   EF1a-sfGFP-Ubc-Neo

   Depositor

   Jonathan Weissman

   Article

   Adamson et al Cell. 2016 Dec 15;167(7):1867-1882.e21.

   Inserts

   sfGFP
   Neo
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Feb. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAV-CAG-oScarlet-WPRE-BC6-pA

   Plasmid

   #220770

   Purpose

   Expression of oScarlet for cell isolation and Projection-TAG BC for multiplex projection tracing

   Depositor

   Vijay Samineni

   Article

   Yang et al Nat Commun. 2025 Jul 1;16(1):5557. doi:

   Insert

   oScarlert-WPRE-BC6
   

   Use

   AAV

   Promoter

   CAG

   Available Since

   July 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pICE-HA-MRE11-H63S

    Plasmid

    #82035

    Purpose

    Plasmid for constitutive or doxycycline-inducible expression of H63S mutant of human MRE11. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.

    Depositor

    Sébastien Britton, Patrick Calsou

    Article

    Chanut et al Nat Commun. 2016 Sep 19;7:12889. doi: 10

    Insert

    MRE11 (MRE11 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    H63S MRE11 mutant

    Promoter

    CMV-tet

    Available Since

    Sept. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLenti-sgRNA(lib)-puro

    Plasmid

    #119976

    Purpose

    Lenti sgRNA cloning backbone with CMV-puro resistance marker. Contains BsmBI sites for insertion of spacer sequences.

    Depositor

    Wensheng Wei

    Article

    Liu et al Nat Biotechnol. 2018 Nov 5. pii: nbt.428

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    March 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSLIK Tgfbr3-HA neo

    Plasmid

    #58508

    Purpose

    Lentiviral expression vector for an inducible HA-tagged mouse Tgfbr3

    Depositor

    Kevin Janes

    Article

    Wang et al Nat Cell Biol. 2014 Apr;16(4):345-56. do

    Insert

    Tgfbr3_HA (Tgfbr3 Mouse)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Available Since

    Aug. 20, 2014

    Availability

    Academic Institutions and Nonprofits only

13. pEM-Cas9HF1

    Plasmid

    #89961

    Purpose

    Modified from pCas9-CR4 (Addgene: 62655) to use the high-fidelity version of Cas9, SpCas9-HF1 (N497A/R661A/Q695A/Q926A) from Kleinstiver et al 2016.

    Depositor

    Michael Lynch

    Article

    Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

    Insert

    Cas9HF1
    

    Expression

    Bacterial

    Mutation

    N497A/R661A/Q695A/Q926A

    Promoter

    pTet

    Available Since

    June 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pl-Synapsin-YFP-NaVII-III

    Plasmid

    #91246

    Purpose

    Expresses YFP-NaVII-III from the Synapsin promoter, for neuron-specific and activity-independent live label of the Axon Initial Segment (AIS)

    Depositor

    Matthew Grubb

    Article

    Dumitrescu et al Front Cell Neurosci. 2016 Nov 23;10:268.

    Insert

    Nav1.2 (Scn2a Rat)
    

    Use

    Lentiviral

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    The insert codes only for the intracellular loop …

    Available Since

    June 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMKO.1 puro GFP shRNA

    Plasmid

    #10675

    Purpose

    Negative control vector for pMKO.1 puro (Addgene plasmid #8452); Contains shRNA against GFP.

    Depositor

    William Hahn

    Article

    Masutomi et al Proc Natl Acad Sci U S A. 2005 Jun 7. 10

    Insert

    GFP shRNA
    

    Use

    RNAi and Retroviral

    Expression

    Mammalian

    Available Since

    Jan. 6, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLKO.1 puro shRNA E-cadherin

    Plasmid

    #18801

    Depositor

    Bob Weinberg

    Article

    Onder et al Cancer Res. 2008 May 15. 68(10):3645-54.

    Insert

    E-cadherin shRNA (CDH1 Human)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    July 22, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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17. AGPAT3_pCSdest

    Plasmid

    #53928

    Depositor

    Roger Reeves

    Article

    Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

    Insert

    AGPAT3 (AGPAT3 Human)
    

    Expression

    Mammalian

    Promoter

    SP6 and CMV

    Available Since

    March 27, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

18. pKnockinDonor-human-p16INK4A-P2A-mCherry

    Plasmid

    #137026

    Purpose

    donor vector to knock in P2A-mCherry to the C-terminus of endogenous human p16INK4A

    Depositor

    Chunliang Li

    Article

    Zhang et al Proc Natl Acad Sci U S A. 2019 Dec 9. pi

    Insert

    p16INK4A-knockin-homology arm-P2A-mCherry
    

    Tags

    P2A-mCherry

    Expression

    Bacterial

    Promoter

    no promoter

    Available Since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCG150_double_phiC31_attB

    Plasmid

    #169693

    Purpose

    cargo vector for the phiC31 system with, unc-119 rescue fragment, no fluorescent marker. Gateway R3R4 sites (for gene of interest insertion)

    Depositor

    Shih-Peng Chan

    Article

    Yang et al Genetics. 2022 Feb 4;220(2):iyab206. doi

    Type

    Empty backbone

    Expression

    Worm

    Available Since

    Oct. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMXs-ms-Sox3

    Plasmid

    #50779

    Purpose

    retroviral expression of mouse Sox3

    Depositor

    Shinya Yamanaka

    Article

    Nakagawa et al Nat Biotechnol. 2008 Jan;26(1):101-6. Ep

    Insert

    Sox3 (Sox3 Mouse)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    Feb. 28, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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