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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 14,065 results for: LEA

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Showing: 7021 - 7040 of 14065 results

  1. pGBW-m4046766

    Plasmid
    #145612
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 helicase
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 helicase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;StrepII
    Expression
    Bacterial
    Mutation
    Escherichia coli recode 1
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145612-plasmid-map-sequence-id-298367

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  2. pGBW-m4046949

    Plasmid
    #145604
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 E (envelope)
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 E (envelope) (E Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;FLAG_2
    Expression
    Bacterial
    Mutation
    wt
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145604-plasmid-map-sequence-id-299796

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  3. pGBW-m4046763

    Plasmid
    #145606
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 M (membrane)
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 M (membrane) (M Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;FLAG_2
    Expression
    Bacterial
    Mutation
    wt
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145606-plasmid-map-sequence-id-298330

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  4. pGBW-m4046804

    Plasmid
    #145595
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 nsp8
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 nsp8 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;StrepII
    Expression
    Bacterial
    Mutation
    Escherichia coli recode 1
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145595-plasmid-map-sequence-id-298362

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  5. pGBW-m4046895

    Plasmid
    #145596
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 3C-like proteinase
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 3C-like proteinase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;StrepII
    Expression
    Bacterial
    Mutation
    wt
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145596-plasmid-map-sequence-id-300253

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  6. pGBW-m4046746

    Plasmid
    #145641
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 ORF10 protein
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 ORF10 protein (ORF10 Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;6xHIS
    Expression
    Bacterial
    Mutation
    wt
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145641-plasmid-map-sequence-id-292574

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  7. pGBW-m4046764

    Plasmid
    #145673
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 2'-O-ribose methyltransferase
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 2'-O-ribose methyltransferase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;StrepII
    Expression
    Bacterial
    Mutation
    wt
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available since
    April 27, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145673-plasmid-map-sequence-id-292487

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  12. pCEP4-BaLgp160-754*

    Plasmid
    #123212
    Purpose
    Mammalian expression plasmid for Env from the BaL HIV-1 isolate; C-terminal truncation
    Depositor
    Erik Procko
    Publication
    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    HIV-1 (BaL) Env
    Tags
    CD5 leader peptide
    Expression
    Mammalian
    Mutation
    Codon-optimized synthetic gene; Premature stop co…
    Promoter
    CMV
    Available since
    May 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    123212-plasmid-map-sequence-id-235146

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  14. pSOMA-T679A-NES

    Plasmid
    #118940
    Purpose
    Used to express the T679A mutant form of the SOMA FRET sensor with a nuclear exclusion signal in Arabidopsis thaliana
    Depositor
    Patrick Krysan
    Publication
    Zaman et al Plant J. 2018 Nov 16. doi: 10.1111/tpj.1
    Insert
    SOMA Sensor for Arabidopsis MAPK activity with T679A Mutation and Nuclear Exclusion Signal
    Expression
    Plant
    Available since
    Feb. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    118940-plasmid-map-sequence-id-232102

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  15. pCEP4-HA-Flag-natT1R2 FS10

    Plasmid
    #113956
    Purpose
    mammalian expression plasmid for FLAG-tagged human T1R2 with signal peptide of influenza hemagglutinin; expression-enhanced variant
    Depositor
    Erik Procko
    Publication
    Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061
    Insert
    T1R2 (TAS1R2 Human)
    Tags
    FLAG and Signal/leader sequence from influenza he…
    Expression
    Mammalian
    Mutation
    delete N-terminal 21 residues, S212N substitution…
    Promoter
    cmv
    Available since
    Feb. 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    113956-plasmid-map-sequence-id-215284

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  16. FGFR2 N549K 3xFlag

    Plasmid
    #110107
    Purpose
    expresses FGFR2 with a single amino acid change in mammalian cells
    Depositor
    Martin Fernandez-Zapico
    Insert
    fibroblast growth factor receptor 2 (FGFR2 Human)
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    mutated Asparagine 549 to Lysine (N549K)
    Promoter
    CMV
    Available since
    May 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    110107-plasmid-map-sequence-id-209777

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  17. hNedd4-2 CS

    Plasmid
    #86041
    Purpose
    full length NeddL3 with C to S mutation with His Xpress tag
    Depositor
    Christie Thomas
    Publication
    Raikwar et al Am J Physiol Renal Physiol. 2010 Aug;299
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    hNeddL3 with C to S mutation in HECT domain (NEDD4 Human)
    Tags
    His and Xpress
    Expression
    Mammalian
    Mutation
    C942S mutation in HECT domain
    Promoter
    CMV
    Available since
    Feb. 8, 2017
    Availability
    Academic Institutions and Nonprofits only
    86041-plasmid-map-sequence-id-171539

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  19. pLSLZ

    Plasmid
    #51495
    Purpose
    pLSL with Zif268:FokI coding sequence followed by approximately 3kb 'filler' sequence, Rep is not included on this plasmid
    Depositor
    Daniel Voytas
    Publication
    Baltes et al Plant Cell. 2014 Jan;26(1):151-63. doi:
    Inserts
    Zif268:FokI
    3kb filler sequence
    Use
    Plant t-dna plasmid
    Tags
    SV40 NLS
    Promoter
    virion-sense LIR and 2x35S
    Available since
    May 1, 2014
    Availability
    Academic Institutions and Nonprofits only
    51495-plasmid-map-sequence-id-78592

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  20. pcDNA3.1-GSDMD_1-275

    Plasmid
    #218230
    Purpose
    Gasdermin-D_1-275 is a pore-forming GSDMD formed by cleavage by host caspases. Transfection in HEK293 cells showed GSDMD 1-275 pores secrete 3CLpro, nucleocapsid; in excess, kills cells by pyroptosis
    Depositor
    Christopher Overall
    Publication
    Grin et al Cell Rep. 2024 Dec 24;43(12):115080. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GSDMD (GSDMD Human)
    Tags
    flag
    Expression
    Mammalian
    Mutation
    construct only codes for amino acids 1-275
    Available since
    July 1, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    218230-plasmid-map-sequence-id-430565

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Showing: 7021 - 7040 of 14065 results