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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 12,130 results for: LEA

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Showing: 7021 - 7040 of 12130 results

  12. pETSUMO2_Nostoc-bGSDM-WELQ

    Plasmid
    #222100
    Purpose
    For expression of a mutant bacterial gasdermin (bGSDM) encoded by a Nostoc species with a WELQut protease cleavage site for controlled activation.
    Depositor
    Philip Kranzusch
    Article
    Johnson et al Nature. 2024 Apr;628(8008):657-663. doi:
    Insert
    Nostoc-bGSDM-WELQ ORF
    Tags
    6xHis-SUMO2
    Expression
    Bacterial
    Mutation
    N-terminal methionine deleted and replaced by sin…
    Promoter
    T7
    Available Since
    July 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    222100-plasmid-map-sequence-id-437100

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  13. BirA*-HA-NES

    Plasmid
    #214473
    Purpose
    Lentiviral vector for expression in mammalian cells of the proximity ligase BirA(R118G) with a C-terminal HA tag followed by a nuclear exclusion sequence.
    Depositor
    Natalie Ahn
    Article
    Miller et al Mol Biol Cell. 2023 Dec 20:mbcE23060255.
    Insert
    BirA(R118G)
    Use
    Gateway Cloning and Lentiviral
    Tags
    HA tag and NES (Nuclear Exclusion Sequence)
    Expression
    Mammalian
    Mutation
    R118G
    Promoter
    EF-1a
    Available Since
    March 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    214473-plasmid-map-sequence-id-425592

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  14. pCB11

    Plasmid
    #201111
    Purpose
    pSR58.6 (Addgene plasmid #63176) modified to include the tetA gene upstream of sfGFP.
    Depositor
    Mary Dunlop
    Article
    Lugagne et al Nat Commun. 2024 Mar 8;15(1):2148. doi:
    Inserts
    CcaR
    tetA(C)
    sfGFP
    Expression
    Bacterial
    Mutation
    C-to-T transition at pos. no. 1049 of tetA(C) lea…
    Promoter
    J23100 and PcpcG2-172
    Available Since
    March 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    201111-plasmid-map-sequence-id-403029

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  18. pBactin-Nedd1-mOrange2-F75A

    Plasmid
    #196862
    Purpose
    Expression of Need1-rCM1 harboring a (F75A) mutation in the rat (r) Centrosomin motif 1 (CM1) and fused to mOrange2. F75A mutatuon renders CM1 incapable of binding and activating gamma-TuRC
    Depositor
    Frank Bradke
    Article
    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16
    Insert
    Nedd1-mOrange2-F75A (Nedd1 Rat)
    Expression
    Mammalian
    Mutation
    F75A in the CM1 domain
    Promoter
    Chicken bactin (plus Chicken bactin intron)
    Available Since
    May 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    196862-plasmid-map-sequence-id-391831

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Showing: 7021 - 7040 of 12130 results