We narrowed to 9,186 results for: mel

1. pGGG_Sr1644-1Sh

   Plasmid

   #164087

   Purpose

   Expresses pGGG_Sr1644-1Sh in plants

   Depositor

   Brande Wulff

   Article

   Yu et al Nat Commun. 2022 Mar 25;13(1):1607. doi:

   Insert

   pGGG_Sr-1644-1Sh
   

   Tags

   none

   Expression

   Bacterial and Plant

   Promoter

   Native promoter

   Available Since

   May 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCRISPaint-mNeon-F-gB-PuroR [M1G]

   Plasmid

   #171803

   Purpose

   Universal donor plasmid for CRISPitope method encoding mNeon fluorescent protein, FLAG tag, HSV-1 glycoprotein B CD8+ T cell epitope [AA: 498-505 (SSIEFARL)] and puromycin resistance cassette [p.M1G]

   Depositor

   Michael Hoelzel

   Article

   Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.

   Insert

   mNeonGreen-FLAG-gB-T2A-PuroR
   

   Use

   Gene tagging

   Mutation

   PuroR: Changed Methionin 1 to Glycine.

   Available Since

   Sept. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCRISPaint-mScarlet-F-Ova-BlastR [M1G]

   Plasmid

   #171817

   Purpose

   Universal donor plasmid for CRISPitope method encoding mScarlet fluorescent protein, FLAG tag, Ovalbumin CD8+ T cell epitope [AA: 257-264 (SIINFEKL)] and blasticidin resistance cassette [p.M1G]

   Depositor

   Michael Hoelzel

   Article

   Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.

   Insert

   mScarlet-F-Ova-T2A-BlastR
   

   Use

   Gene tagging

   Mutation

   BlastR: Changed Methionin 1 to Glycine.

   Available Since

   July 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCRISPaint-mScarlet-F-gB-BlastR [M1G]

   Plasmid

   #171816

   Purpose

   Universal donor plasmid for CRISPitope method encoding mScarlet fluorescent protein, FLAG tag, HSV-1 glycoprotein B CD8+ T cell epitope [AA: 498-505 (SSIEFARL)] and blasticidin resistance cassette [p.

   Depositor

   Michael Hoelzel

   Article

   Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.

   Insert

   mScarlet-F-gB-T2A-BlastR
   

   Use

   Gene tagging

   Mutation

   BlastR: Changed Methionin 1 to Glycine.

   Available Since

   July 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCRISPaint-mScarlet-F-hgp100-BlastR [M1G]

   Plasmid

   #171815

   Purpose

   Universal donor plasmid for CRISPitope method encoding mScarlet fluorescent protein, FLAG tag, human gp100 CD8+ T cell epitope [AA: 25-33 (KVPRNQDWL)] and blasticidin resistance cassette [p.M1G]

   Depositor

   Michael Hoelzel

   Article

   Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.

   Insert

   mScarlet-F-hgp100-T2A-BlastR
   

   Use

   Gene tagging

   Mutation

   BlastR: Changed Methionin 1 to Glycine.

   Available Since

   July 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCRISPaint-mScarlet-F-gB-PuroR [M1G]

   Plasmid

   #171812

   Purpose

   Universal donor plasmid for CRISPitope method encoding mScarlet fluorescent protein, FLAG tag, HSV-1 glycoprotein B CD8+ T cell epitope [AA: 498-505 (SSIEFARL)] and puromycin resistance cassette [p.M1

   Depositor

   Michael Hoelzel

   Article

   Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.

   Insert

   mScarlet-F-gB-T2A-PuroR
   

   Use

   Gene tagging

   Mutation

   PuroR: Changed Methionin 1 to Glycine.

   Available Since

   July 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCRISPaint-mScarlet-F-mgp100-PuroR [M1G]

   Plasmid

   #171811

   Purpose

   Universal donor plasmid for CRISPitope method encoding mScarlet fluorescent protein, FLAG tag, murine gp100 CD8+ T cell epitope [AA: 25-33 (EGSRNQDWL)] and puromycin resistance cassette [p.M1G]

   Depositor

   Michael Hoelzel

   Article

   Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.

   Insert

   mScarlet-F-mgp100-T2A-PuroR
   

   Use

   Gene tagging

   Mutation

   PuroR: Changed Methionin 1 to Glycine.

   Available Since

   July 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCRISPaint-mScarlet-F-hgp100-PuroR [M1G]

   Plasmid

   #171810

   Purpose

   Universal donor plasmid for CRISPitope method encoding mScarlet fluorescent protein, FLAG tag, human gp100 CD8+ T cell epitope [AA: 25-33 (KVPRNQDWL)] and puromycin resistance cassette [p.M1G]

   Depositor

   Michael Hoelzel

   Article

   Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.

   Insert

   mScarlet-F-hgp100-T2A-PuroR
   

   Use

   Gene tagging

   Mutation

   PuroR: Changed Methionin 1 to Glycine.

   Available Since

   July 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCRISPaint-mNeon-F-Ova-BlastR [M1G]

   Plasmid

   #171808

   Purpose

   Universal donor plasmid for CRISPitope method encoding mNeon fluorescent protein, FLAG tag, Ovalbumin CD8+ T cell epitope [AA: 257-264 (SIINFEKL)] and blasticidin resistance cassette [p.M1G

   Depositor

   Michael Hoelzel

   Article

   Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.

   Insert

   mNeonGreen-F-Ova-T2A-BlastR
   

   Use

   Gene tagging

   Mutation

   BlastR: Changed Methionin 1 to Glycine.

   Available Since

   July 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCRISPaint-mNeon-F-gB-BlastR [M1G]

    Plasmid

    #171807

    Purpose

    Universal donor plasmid for CRISPitope method encoding mNeon fluorescent protein, FLAG tag, HSV-1 glycoprotein B CD8+ T cell epitope [AA: 498-505 (SSIEFARL)] and blasticidin resistance cassette [p.M1G

    Depositor

    Michael Hoelzel

    Article

    Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.

    Insert

    mNeonGreen-F-gB-T2A-BlastR
    

    Use

    Gene tagging

    Mutation

    BlastR: Changed Methionin 1 to Glycine.

    Available Since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCRISPaint-mNeon-F-hgp100-BlastR [M1G]

    Plasmid

    #171806

    Purpose

    Universal donor plasmid for CRISPitope method encoding mNeon fluorescent protein, FLAG tag, human gp100 CD8+ T cell epitope [AA: 25-33 (KVPRNQDWL)] and blasticidin resistance cassette [p.M1G]

    Depositor

    Michael Hoelzel

    Article

    Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.

    Insert

    mNeonGreen-F-hgp100-T2A-BlastR
    

    Use

    Gene tagging

    Mutation

    BlastR: Changed Methionin 1 to Glycine.

    Available Since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCRISPaint-mNeon-F-Ova-PuroR [M1G]

    Plasmid

    #171804

    Purpose

    Universal donor plasmid for CRISPitope method encoding mNeon fluorescent protein, FLAG tag, Ovalbumin CD8+ T cell epitope [AA: 257-264 (SIINFEKL)] and puromycin resistance cassette [p.M1G]

    Depositor

    Michael Hoelzel

    Article

    Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.

    Insert

    mNeonGreen-FLAG-Ova-T2A-PuroR
    

    Use

    Gene tagging

    Mutation

    PuroR: Changed Methionin 1 to Glycine.

    Available Since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLT3REVIR_MARK3 2573

    Plasmid

    #107233

    Purpose

    shRNA 2573. Do not use Evos or Acurri on these cells. Assess by FACS specifically with Venus and DsRed lazers. Tet-ON-all-in-one

    Depositor

    Alex Kentsis

    Article

    Brown et al Cancer Discov. 2018 Apr;8(4):478-497. do

    Insert

    MARK3 shRNA (22 nt) (MARK3 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    March 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLT3REVIR_MARK3 1122

    Plasmid

    #107232

    Purpose

    shRNA 1122. Do not use Evos or Acurri on these cells. Assess by FACS specifically with Venus and DsRed lazers. Tet-ON-all-in-one

    Depositor

    Alex Kentsis

    Article

    Brown et al Cancer Discov. 2018 Apr;8(4):478-497. do

    Insert

    MARK3 shRNA (22 nt) (MARK3 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    March 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. P3-dCas9-Tet1-GFP-Puro

    Plasmid

    #190728

    Purpose

    Expression of human spdCas9-Tet1 fusion for targeted DNA methylation in mammalian cell. EGFP-puromycin double marker under an independent CMV promoter. Cloning backbone for spgRNA (BbsI)

    Depositor

    Maja Jagodic

    Article

    Pahlevan Kakhki et al Nat Commun. 2024 Jul 30;15(1):6419. doi:

    Insert

    dCas9-tet1
    

    Use

    CRISPR

    Tags

    3XFLAG and SV40 NLS

    Expression

    Mammalian

    Mutation

    Changed Pro434 codon from CCG to CCC and Gly468 f…

    Promoter

    pCAG

    Available Since

    July 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLenti-CD74-ROS1 nonmutant

    Plasmid

    #183813

    Purpose

    Express CD74-ROS1 fusion (C6;R34) in mammalian cells

    Depositor

    Aaron Hata

    Article

    Lin et al Clin Cancer Res. 2021 May 15;27(10):2899

    Inserts

    ROS Proto-Oncogene 1, Receptor Tyrosine Kinase (ROS1 Human)
    CD74 molecule (CD74 Human)
    

    Use

    Lentiviral

    Tags

    The fusion protein of CD74 exon 1-6 and ROS1 exon…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLenti-CD74-ROS1 G2032R

    Plasmid

    #183814

    Purpose

    Express CD74-ROS1 fusion (C6;R34) harboring ROS1 G2032R mutation in mammalian cells

    Depositor

    Aaron Hata

    Article

    Lin et al Clin Cancer Res. 2021 May 15;27(10):2899

    Inserts

    ROS Proto-Oncogene 1, Receptor Tyrosine Kinase (ROS1 Human)
    CD74 molecule (CD74 Human)
    

    Use

    Retroviral

    Tags

    The fusion protein of CD74 exon 1-6 and ROS1 exon…

    Expression

    Mammalian

    Mutation

    G2032R

    Promoter

    CMV

    Available Since

    April 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCDNA3-Flag-SPRTN-E112A

    Plasmid

    #110215

    Purpose

    Expression in mammalian cells of of full-length SPRTN protein carrying E112A mutation, a catalytically-dead mutant, and Flag-tag at N-terminus.

    Depositor

    Ivana Marinovic-Terzic, Kristijan Ramadan

    Article

    Lessel et al Nat Genet. 2014 Nov;46(11):1239-44. doi:

    Insert

    SPRTN (SPRTN Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    E112A, P296L (see depositor comments below)

    Available Since

    May 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. delta PE OCT4-GFP-2A-PURO transgenic reporter

    Plasmid

    #52380

    Purpose

    BAC recombineering generated construct, used as a reporter for OCT4 expression

    Depositor

    Jacob Hanna

    Article

    Gafni et al Nature. 2013 Dec 12;504(7479):282-6. doi

    Inserts

    GFP-2A-puro
    OCT4 (POU5F1 Human)
    

    Expression

    Mammalian

    Mutation

    deleted -1620 - -1011 upstream from OCT4 ATG

    Available Since

    June 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. delta DE OCT4-GFP-2A-PURO transgenic reporter

    Plasmid

    #52381

    Purpose

    BAC recombineering generated construct, used as a reporter for OCT4 expression

    Depositor

    Jacob Hanna

    Article

    Gafni et al Nature. 2013 Dec 12;504(7479):282-6. doi

    Inserts

    GFP-2A-puro
    OCT4 (POU5F1 Human)
    

    Expression

    Mammalian

    Mutation

    deleted -3194 - -1768 upstream from OCT4 ATG

    Promoter

    OCT4

    Available Since

    June 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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