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Pooled Library#170511PurposeCRISPR library targeting mouse genesDepositorExpressionMammalianSpeciesSyntheticUseCRISPR and LentiviralAvailable sinceSept. 9, 2021AvailabilityAcademic Institutions and Nonprofits only
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p404TEF1
Plasmid#15972DepositorInsertTEF1 promoter + polylinker + CYC1 terminator (TEF1 Budding Yeast)
UseTagsExpressionBacterial and YeastMutationTEF1 promoter is between SacI and XbaI restrictio…PromoterAvailable sinceNov. 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
pET His6 GST TEV LIC cloning vector (1G)
Plasmid#29655DepositorTypeEmpty backboneUseTags6xHis, GST, and TEV cleavage siteExpressionBacterialMutationPromoterT7-lacO (lactose/IPTG inducible)Available sinceJune 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_SAMHD1_WT
Plasmid#81899PurposeGateway Donor vector containing SAMHD1 , part of the Target Accelerator Plasmid Collection.DepositorInsertSAMHD1 (SAMHD1 Human)
UseGateway entry vectorTagsExpressionMutationG404E; A546VPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLX304-ARNT-d414
Plasmid#203579PurposeExpresses a functionally inactive version of ARNT with 5’ 414 bp deletion [Δ414] in mammalian cells.DepositorInsertARNT (ARNT Human)
UseLentiviralTagsV5ExpressionMammalianMutation5' 414 basepair deletion [Δ414]PromoterCMVAvailable sinceFeb. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
DNA-PKcs PQR>ala
Plasmid#83319Purposehuman DNA-PKcs PQR>alaDepositorInserthuman DNA-PKcs (PRKDC Human)
UseTagsExpressionMammalianMutation5 phosphorylation sites in the PQR cluster substi…PromoterAvailable sinceAug. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-UGAcS
Plasmid#172135Purposemammalian expression plasmid for a UDP-GlcNAc sensorDepositorInsertUGAcS
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRK5-HA-Ubiquitin-K11
Plasmid#22901DepositorInsertUbiquitin C (UBC Human)
UseTagsHAExpressionMammalianMutationK11 only, all other lysines mutated to argininesPromoterAvailable sinceJan. 22, 2010AvailabilityAcademic Institutions and Nonprofits only -
pFLAG-GATA2
Plasmid#1418DepositorInsertGATA-2 (GATA2 Human)
UseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceMarch 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-mSmo
Plasmid#25395DepositorInsertmouse Smoothened cDNA (Smo Mouse)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceJune 22, 2010AvailabilityAcademic Institutions and Nonprofits only -
Snail_pGL2
Plasmid#31694DepositorInsertSnail (SNAI1 Human)
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 21, 2011AvailabilityAcademic Institutions and Nonprofits only -
Tet-inducible mCherry reporter
Plasmid#64128PurposePhotoactivatable transcription system. mCherry reporter under the control of a tet-inducible promoterDepositorInsertmCherry
UseTagsExpressionMammalianMutationPromoterTetOx13-CMVAvailable sinceApril 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO/Strep-HA-ZAK alpha
Plasmid#141193PurposeExpresses Strep-HA-ZAK alpha in mammalian cells, can be used to make inducible cell lineDepositorInsertMAP3K20 (MAP3K20 Human)
UseTagsStrep, HAExpressionMammalianMutationPromoterCMVAvailable sinceJune 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCW-Cas9-2A-EGFP
Plasmid#167928PurposeDoxycycline-inducible Cas9 expression tracked by EGFP fluorescence marker.DepositorInsertT2A-EGFP
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromotertTRE, hPGKAvailable sinceMay 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHH21-BNA-Luc
Plasmid#187924PurposeExpresses Luciferase reporter RNA construct under the 5' and 3' UTRs of NA segment of Influenza B/Brisbane/60/2008 virus by RNA polymerase I in mammalian cellsDepositorInsertReporter RNA (RNA encoding firefly luciferase under the viral promoter of segment 6 of Influenza B/Brisbane/60/2008 virus)
UseTagsExpressionMammalianMutationPromoterRNA polymease I PromoterAvailable sinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
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His-GFP-Clathrin
Plasmid#170858PurposeMammalian expression of His-GFP-Clathrin.DepositorInsertClathrin light polypeptide (Lca) (Clta Mouse)
UseTags(His)6 and EGFPExpressionMammalianMutationPromoterCMVAvailable sinceJune 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pdCas9-DNMT3A-EGFP
Plasmid#71666PurposeExpression of human codon-optimized dCas9-DNMT3A fusion with T2A-EGFP for targeted DNA methylation in mammalian cells; cloning backbone for sgRNADepositorInsertS. pyogenes dCas9 fused with the catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-EGFP (DNMT3A S. pyogenes, Human, Synthetic)
UseCRISPRTags3xFLAG, SV40 NLS, and T2A-EGFPExpressionMammalianMutationD10A and H840A in S.pyogenes Cas9PromoterCBhAvailable sinceMarch 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-fDIO-eGFP-WPRE
Plasmid#203843PurposeFlp-dependent EGFP expressionDepositorInsertEGFP
UseAAVTagsExpressionMutationPromoterAvailable sinceOct. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF2053
Plasmid#142912PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertBCL6 (BCL6 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceApril 27, 2023AvailabilityAcademic Institutions and Nonprofits only