167,601 results

1. pcDNA3.1_sfCherry2(1-10)

   Plasmid

   #82602

   Purpose

   Expresses sfCherry2(1-10) fusion in mammalian cells

   Depositor

   Bo Huang

   Article

   Feng et al Nat Commun. 2017 Aug 29;8(1):370. doi: 1

   Insert

   sfCherry2(1-10)
   

   Expression

   Mammalian

   Mutation

   changed Glu 118 to Gln, changed Thr 128 to Ile

   Promoter

   CMV

   Available Since

   Jan. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET-AtC60αβC20NtL

   Plasmid

   #160876

   Purpose

   Expression of N. tabacum Rubisco large subunit (rbcL) and A. thaliana Rubisco chaperone protiens cpn60α, cpn60β, cpn20 in E. coli

   Depositor

   Maureen Hanson

   Article

   Lin et al Nat Plants. 2020 Oct;6(10):1289-1299. do

   Insert

   rbcL, cpn60α, cpn60β, cpn20
   

   Expression

   Bacterial

   Promoter

   rbcL: T7, chaperones: T7

   Available Since

   Nov. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. Dendra2-H2B-6

   Plasmid

   #57724

   Purpose

   Localization: Nucleus Histones, Excitation: 490 / 553, Emission: 507 / 573

   Depositor

   Michael Davidson

   Insert

   H2B (H2BC11 Human)
   

   Tags

   Dendra2

   Expression

   Mammalian

   Mutation

   D26G and V119I in H2B

   Promoter

   CMV

   Available Since

   Jan. 13, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

4. pV132-vCas9

   Plasmid

   #203223

   Purpose

   Mammalian expression of vCas9

   Depositor

   Phil Sharp, Robert Langer, Vikash Chauhan

   Article

   Chauhan et al Proc Natl Acad Sci U S A. 2023 Mar 14;12

   Insert

   vCas9
   

   Use

   AAV and CRISPR

   Expression

   Mammalian

   Mutation

   S55R R976A K1003A T1314R

   Promoter

   CBh

   Available Since

   Aug. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEMG-GRIS

   Plasmid

   #225134

   Purpose

   For genome editing in P. putida KT2440, rhamnose is used for the induction of I-sceI gene. Gm.

   Depositor

   Lars Blank

   Article

   Meng et al Microb Biotechnol. 2024 Jul;17(7):e14531

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   Oct. 10, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pCDF-NtXNtS1R1R2B2

   Plasmid

   #160882

   Purpose

   Expression of N. tabacum Rubisco small subunit (rbcS-S1), Rubisco chaperone protiens rbcX, raf1, bsd2 and raf2 in E. coli.

   Depositor

   Maureen Hanson

   Article

   Lin et al Nat Plants. 2020 Oct;6(10):1289-1299. do

   Insert

   rbcS-S1, rbcX, raf1, raf2, bsd2
   

   Expression

   Bacterial

   Promoter

   rbcS-S1: T7, chaperones: T7

   Available Since

   Dec. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBiFCt-2in1-CC

   Plasmid

   #105114

   Purpose

   ratiometric BiFC analysis in plants (transient transformation)

   Depositor

   Christopher Grefen

   Article

   Grefen et al Biotechniques. 2012 Oct 2;53(5):311-14.

   Type

   Empty backbone

   Tags

   cYFP-Myc/nYFP-HA

   Expression

   Plant

   Available Since

   Feb. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. mFGF5

   Plasmid

   #22087

   Depositor

   Gail Martin

   Insert

   FGF5 (Fgf5 Mouse)
   

   Use

   Bacterial

   Mutation

   V134G

   Available Since

   Oct. 1, 2009

   Availability

   Academic Institutions and Nonprofits only

9. tdTomato-Farnesyl-5

   Plasmid

   #58092

   Purpose

   Localization: Membrane, Excitation: 554, Emission: 581

   Depositor

   Michael Davidson

   Insert

   Farnesyl (HRAS Human)
   

   Tags

   tdTomato

   Expression

   Mammalian

   Mutation

   encodes final 20 aa of NM_001130442.1

   Promoter

   CMV

   Available Since

   Dec. 23, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3-mCherry-Rheb

    Plasmid

    #192436

    Purpose

    Encodes mCherry-tagged wild-type Rheb.

    Depositor

    Jin Zhang

    Article

    Zhong et al Cell Chem Biol. 2022 Jun 16;29(6):1037-1

    Insert

    mCherry-Rheb (RHEB Human)
    

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCW-eGFP-FHA

    Plasmid

    #114129

    Purpose

    Lentiviral vector for inducible expression of eGFP-RNF8 FHA domain fusion; 53BP1-independent recruitment to damaged chromatin

    Depositor

    Daniel Durocher

    Article

    Noordermeer et al Nature. 2018 Aug;560(7716):117-121. doi:

    Insert

    RNF8 FHA domain (amino acids 2-160) (RNF8 Human)
    

    Use

    Lentiviral; Doxycycline inducible

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    aa 2-160

    Promoter

    TRE promoter, Tet ON

    Available Since

    Aug. 28, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pAGT9403

    Plasmid

    #192352

    Purpose

    L0 MoClo-compatible GoldenGate-module of the NOS terminator (tNOS) with stop

    Depositor

    Tom Schreiber

    Article

    Schindele et al Plant Biotechnol J. 2023 Mar;21(3):457-4

    Insert

    TTCG_Stop-tNOS_CGCT
    

    Expression

    Plant

    Available Since

    Nov. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pre_IV_CS10_1545_v1

    Plasmid

    #126861

    Purpose

    4x 1545 b long scaffolds divided by Zn-dependent self-cleaving DNAzyme cassettes, from compatibility group 10, made by method IV, yields linear 1033 b long scaffolds after Zn incubation

    Depositor

    Hendrik Dietz

    Article

    Engelhardt et al ACS Nano. 2019 Apr 22. doi: 10.1021/acsn

    Insert

    IV_CS10_1545_v1
    

    Use

    Synthetic Biology

    Available Since

    July 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. TFORF1271

    Plasmid

    #143780

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    HLTF (HLTF Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    June 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA-ncpGCaMP6s

    Plasmid

    #113674

    Purpose

    A topological variant of GCaMP6s

    Depositor

    Robert Campbell

    Article

    Qian et al Chembiochem. 2018 Jun 22. doi: 10.1002/c

    Insert

    ncpGCaMP6s
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMG059 MBP-CREB-His

    Plasmid

    #154076

    Purpose

    Expresses MBP-CREB-His (human CREB as a fusion protein with MBP and His- tags) in E.coli

    Depositor

    Jesse Zalatan

    Article

    Gavagan et al Cell Syst. 2020 Jun 24;10(6):515-525.e5.

    Insert

    CREB1 (CREB1 Human)
    

    Tags

    His6 and MBP

    Expression

    Bacterial

    Promoter

    Tac

    Available Since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. ActTS-GR

    Plasmid

    #79774

    Purpose

    FRET-based actinin tension sensors

    Depositor

    Tetsuya Kitaguchi

    Article

    Yamashita et al Sci Rep. 2016 Jun 23;6:28535. doi: 10.10

    Insert

    ActTS-GR
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRU41

    Plasmid

    #167663

    Purpose

    ENTRY vector for gRNA1 cloning under pAtU6 promoter

    Depositor

    Niko Geldner

    Article

    Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

    Insert

    pAtU6
    

    Use

    CRISPR

    Available Since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAG-hPPP3CB-FLAG

    Plasmid

    #179135

    Purpose

    Expression vector of human protein phosphatase 3, catalytic subunit, beta isoform (PPP3CB) tagged with FLAG at C-terminus, CAG promoter, rabbit globin poly(A) signal.

    Depositor

    Masahito Ikawa

    Article

    Miyata et al Proc Natl Acad Sci U S A. 2021 Aug 31;11

    Insert

    protein phosphatase 3, catalytic subunit, beta isoform (PPP3CB Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    Jan. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. FLAG.PKCepsilon

    Plasmid

    #10795

    Depositor

    Alex Toker

    Article

    Cenni et al Biochem J. 2002 May 1. 363(Pt 3):537-45.

    Insert

    PKC epsilon (PRKCE Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    April 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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