165,174 results

1. CROPseq-EFS-HypaSpCas9-P2A-puro

   Plasmid

   #106965

   Purpose

   single-vector CROPseq system with high fidelity SpCas9 (HypaSpCas9) for use in the CROPseq CRISPR knockout screen

   Depositor

   Alex Hewitt

   Insert

   HypaSpCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EFS

   Available since

   April 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. trPAGmEos3.2

   Plasmid

   #203752

   Purpose

   Encodes the transmembrane domain from PAG/CSK, including its 2 palmitoylation sites, with mEos3.2 fused on the C terminus. Used as a fluorescent plasma membrane probe.

   Depositor

   Sarah Veatch

   Article

   Shelby et al Nat Chem Biol. 2023 Jun;19(6):750-758. d

   Insert

   trPAG (PAG1 Human)
   

   Use

   Tags

   mEos3.2

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDB4581

   Plasmid

   #171124

   Purpose

   A kanMX-marked pFA6a based plasmid, which has a 7-aa linker, a 16-aa XTEN16 linker, three tandam repeats of sAID. It serves as the template for 3_sAID degron tagging at the C-terminus.

   Depositor

   Li-Lin Du

   Article

   Zhang et al G3 (Bethesda). 2022 Jan 4;12(1):jkab393.

   Insert

   3_sAID
   

   Use

   Other

   Tags

   Expression

   Mutation

   non applicable

   Promoter

   Available since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDONRPEX18Tp-SceI-pheS

   Plasmid

   #111608

   Purpose

   Gateway compatible allelic exchange vector for Burkholderia sp.

   Depositor

   Tim Tolker-Nielsen

   Article

   Fazli et al Appl Environ Microbiol. 2015 Jun;81(11):

   Type

   Empty backbone

   Use

   Allelic exchange in burkholderia sp.

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLentiCMVNeoDEST(705-1)-hAXL

   Plasmid

   #124796

   Purpose

   Expresses AXL in mammalian cells

   Depositor

   Kenneth Pienta

   Article

   Axelrod et al Mol Cancer Res. 2019 Feb;17(2):356-369.

   Insert

   AXL (AXL Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDawn-Ag43

   Plasmid

   #107742

   Purpose

   Expresses Ag43 under the control of a blue-light transcriptional regulator

   Depositor

   Ingmar Riedel-Kruse

   Article

   Jin et al Proc Natl Acad Sci U S A. 2018 Apr 3;115

   Insert

   Ag43 (flu )
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   removed all pstI cut sites with silent mutations,…

   Promoter

   pLambda

   Available since

   April 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. p-EF1a-CreERT2-3Xflag-T2A-eBFP2

   Plasmid

   #170186

   Purpose

   This Cre-ERT2 expressing construct can be used to inducibly recombine loxp sites. It can be used with poly-loxP containing plasmids to generate timestamp barcodes useful for linage tracing

   Depositor

   Bradley Bernstein

   Article

   Kim et al Cell Rep. 2020 Oct 6;33(1):108222. doi:

   Insert

   CreERT2-T2A-eBFP2
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EF1 alpha

   Available since

   July 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAG-luciferase

   Plasmid

   #55764

   Purpose

   luciferase reporter for in vivo bioluminescence imaging

   Depositor

   Snorri Thorgeirsson

   Article

   Lee et al Cancer Res. 2010 Nov 1;70(21):8264-9. do

   Insert

   CMV enhancer/chicken beta actin promoter
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV enhancer/chicken beta actin promoter

   Available since

   Sept. 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGBKT7-GW

   Plasmid

   #61703

   Purpose

   Gateway compatible Y2H vector - BD

   Depositor

   Yuhai Cui

   Article

   Lu et al Plant J. 2010 Jan;61(2):259-70. doi: 10.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBJ089_zACNE20_myl7_Voltron1_P2A_jGCaMP7f

    Plasmid

    #209283

    Purpose

    Expresses Voltron1 and jGCaMP7f in the zebrafish heart

    Depositor

    Adam Cohen

    Article

    Jia et al Nature. 2023 Oct;622(7981):149-155. doi:

    Insert

    Voltron1_P2A_jGCaMP7f
    

    Use

    Zebrafish expression

    Tags

    Expression

    Mutation

    Promoter

    zACNE20_myl7

    Available since

    Nov. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTU1-A-SP44-mScarlet-I

    Plasmid

    #163756

    Purpose

    Expresses Streptomyces codon-optimised mScarlet-I

    Depositor

    Paul Freemont, Simon Moore

    Article

    Moore et al ACS Synth Biol. 2021 Feb 19;10(2):402-41

    Insert

    mScarlet-I
    

    Use

    Synthetic Biology

    Tags

    Flag and FlAsH tags

    Expression

    Bacterial

    Mutation

    Promoter

    SP44

    Available since

    July 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pPROBE-NT

    Plasmid

    #37818

    Depositor

    Steven Lindow

    Article

    Miller et al Mol Plant Microbe Interact. 2000 Nov;13(

    Insert

    Promotorless gfp reporter gene
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 23, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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13. pInducer20 EGFP-TEADi

    Plasmid

    #140145

    Purpose

    Tetracycline-inducible TEADi (GFP-tagged inhibitor of the interaction of YAP1 and TAZ with TEAD transcription factors with nuclear localization signal)

    Depositor

    Ramiro Iglesias-Bartolome

    Article

    Yuan et al Nat Commun. 2020 Mar 19;11(1):1472. doi:

    Insert

    EGFP TEADi
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHR-SFFV-dCas9-BFP

    Plasmid

    #46910

    Purpose

    Human expression vector containing SFFV promoter, dCas9 that is fused to 2x NLS and tagBFP

    Depositor

    Jonathan Weissman, Stanley Qi

    Article

    Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008

    Insert

    dCas9-BFP fusion
    

    Use

    CRISPR and Lentiviral

    Tags

    2xNLS, BFP, and HA

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    Oct. 1, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. pENTR-MBD1

    Plasmid

    #47057

    Purpose

    Expresses methyl-CpG binding domain protein 1

    Depositor

    Huda Zoghbi

    Article

    Sakai et al Sci Transl Med. 2011 Jun 8;3(86):86ra49.

    Insert

    Methyl-CpG Binding Domain Protein 1 (MBD1 Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

16. pMGE-T7

    Plasmid

    #153021

    Purpose

    Single-plasmid multi-gene expression system. Each genes under the control of a T7 and the lacO promoter sequences.

    Depositor

    Vito Valiante

    Article

    Kufs et al ACS Synth Biol. 2020 Jun 11. doi: 10.102

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. Cytosolic Syk Biosensor

    Plasmid

    #125729

    Purpose

    FRET biosensor for studying SYK activities in cells

    Depositor

    Yingxiao Wang

    Article

    Xiang et al Cell Mol Bioeng. 2011 Dec;4(4):670-677.

    Insert

    SYK biosensor (ZAP70 Synthetic)
    

    Use

    Tags

    6xHistine Tag at the N-terminal.

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pmiRFP720-N1

    Plasmid

    #136560

    Purpose

    A cytoplasmic expression of a monomeric phytochrome-based near-infrared fluorescent protein miRFP720

    Depositor

    Vladislav Verkhusha

    Article

    Matlashov et al Nat Commun. 2020 Jan 13;11(1):239. doi:

    Insert

    miRFP720
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV.CMV.HI.eGFP-Cre.WPRE.SV40 (AAV2)

    Viral Prep

    #105545-AAV2

    Purpose

    Ready-to-use AAV2 particles produced from pAAV.CMV.HI.eGFP-Cre.WPRE.SV40 (#105545). In addition to the viral particles, you will also receive purified pAAV.CMV.HI.eGFP-Cre.WPRE.SV40 plasmid DNA. CMV-driven EGFP-Cre expression. These AAV preparations are suitable purity for injection into animals.

    Depositor

    James M. Wilson

    Promoter

    HI

    Tags

    EGFP

    Available since

    Oct. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

20. pCALNL-DsRed

    Plasmid

    #13769

    Purpose

    Cre recombinase-dependent expression of DsRed, i.e. DsRed is only expressed in the presence of Cre due to a transcriptional stop in between the loxP sites.

    Depositor

    Connie Cepko

    Article

    Matsuda et al Proc Natl Acad Sci U S A. 2007 Jan 16;10

    Insert

    DsRed2
    

    Use

    Cre/Lox; Flp/frt

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    April 20, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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