We narrowed to 15,981 results for: LEA

1. pLKO_HSV.S.V5_mCherry-NLS

   Plasmid

   #178278

   Purpose

   Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

   Depositor

   Brian Brown

   Article

   Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

   Insert

   nPC Tagged mCherry-NLS
   

   Use

   Lentiviral

   Tags

   HSV.S.V5 and Nuclear Localization Signal

   Promoter

   eF1a

   Available Since

   Jan. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLKO_HSV.Ollas.V5_mCherry-NLS

   Plasmid

   #178277

   Purpose

   Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

   Depositor

   Brian Brown

   Article

   Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

   Insert

   nPC Tagged mCherry-NLS
   

   Use

   Lentiviral

   Tags

   HSV.Ollas.V5 and Nuclear Localization Signal

   Promoter

   eF1a

   Available Since

   Jan. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLKO_HSV.NWS.S_mCherry-NLS

   Plasmid

   #178273

   Purpose

   Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

   Depositor

   Brian Brown

   Article

   Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

   Insert

   nPC Tagged mCherry-NLS
   

   Use

   Lentiviral

   Tags

   HSV.NWS.S and Nuclear Localization Signal

   Promoter

   eF1a

   Available Since

   Jan. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pYPQ239-RR

   Plasmid

   #108862

   Purpose

   FnCpf1 Gateway entry plasmid with N623R and K687R mutations

   Depositor

   Yiping Qi

   Article

   Zhong et al Mol Plant. 2018 Jul 2;11(7):999-1002. do

   Insert

   FnCpf1-RR (FnCpf1 with N623R and K687R mutations)
   

   Use

   CRISPR; Gateway compatible cpf1 entry clone

   Tags

   NLS

   Expression

   Plant

   Mutation

   N623R and K687R, Cpf1 is rice codon optimized

   Available Since

   Aug. 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. PET-SpCas9-HF1-plus-NLS-6xHis

   Plasmid

   #207381

   Purpose

   Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   SpCas9-HF1-plus
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   N497A, Q695A, Q926A, amino acids 1005-1013 replac…

   Promoter

   T7

   Available Since

   Oct. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. PET-B-SpCas9-HF1-NLS-6xHis

   Plasmid

   #207385

   Purpose

   Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   B-SpCas9-HF1
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   N497A, R661A, Q695A, Q926A, amino acids 1005-1013…

   Promoter

   T7

   Available Since

   Sept. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEGFP-LC3-K51A

   Plasmid

   #155265

   Purpose

   Mammalian expression of rat LC3 fused to EGFP

   Depositor

   David Rubinsztein

   Article

   Puri et al Dev Cell. 2020 Apr 20;53(2):154-168.e6.

   Insert

   microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)
   

   Tags

   pEGFP

   Expression

   Mammalian

   Mutation

   Lysine 51 to Alanine

   Available Since

   May 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEGFP-LC3-R70A

   Plasmid

   #155264

   Purpose

   Mammalian expression of rat LC3 fused to EGFP

   Depositor

   David Rubinsztein

   Article

   Puri et al Dev Cell. 2020 Apr 20;53(2):154-168.e6.

   Insert

   microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)
   

   Tags

   pEGFP

   Expression

   Mammalian

   Mutation

   Arginine 70 to Alanine

   Available Since

   May 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. EF1a_DIO_R133C-HaloTag

   Plasmid

   #164064

   Purpose

   Double-Floxed Inverted Open reading frame for mouseMeCP2(alpha) carrying R133C mutation

   Depositor

   Nathaniel Heintz

   Article

   Piccolo et al Elife. 2019 Dec 23;8. pii: 51449. doi: 1

   Insert

   MeCP2 Isoform 1 R133C (Mecp2 Mouse)
   

   Use

   AAV

   Tags

   HaloTag

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Feb. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV_UdgX-EE-UdgX-NG-nCas9-RBMX

    Plasmid

    #163571

    Purpose

    Mammalian CG-to-GC base editing

    Depositor

    David Liu

    Article

    Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

    Insert

    UdgX-EE-UdgX-NG-nCas9-RBMX
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    nCas9 (D10A)EE (R126E, R132E)NG (L111R, D1135V, G…

    Available Since

    Dec. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. HOXB3 (human) FLAG pSP65

    Plasmid

    #8523

    Depositor

    Corey Largman

    Article

    Shen et al Nucleic Acids Res 1996 Mar 1;24(5):898-9

    Insert

    HOXB3 (HOXB3 Human)
    

    Use

    In vitro transcription/translation

    Tags

    FLAG

    Expression

    Bacterial

    Mutation

    Contains 5 additional amino acids before ATG from…

    Available Since

    May 30, 2006

    Availability

    Academic Institutions and Nonprofits only

12. pcDNA3.1-Ctdnep1_D67E-2xFlag

    Plasmid

    #196519

    Purpose

    Expression of Ctdnep1_D67E-2xFlag

    Depositor

    Edgar Gomes

    Article

    Calero-Cuenca et al Curr Biol. 2021 Apr 12;31(7):1521-1530.e

    Insert

    Ctdnep1_D67E-2xFlag (CTDNEP1 Human)
    

    Tags

    2xFlag

    Expression

    Mammalian

    Mutation

    D67E mutation (phosphastase dead)

    Promoter

    CMV promoter; T7 promoter

    Available Since

    March 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1+CeGFP-Ctdnep1WTsiR

    Plasmid

    #196517

    Purpose

    Expression of Ctdnep1WT-GFP

    Depositor

    Edgar Gomes

    Article

    Calero-Cuenca et al Curr Biol. 2021 Apr 12;31(7):1521-1530.e

    Insert

    Ctdnep1WTsiR (CTDNEP1 Human)
    

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Silent mutations for siRNA resistance

    Promoter

    CMV promoter; T7 promoter

    Available Since

    Feb. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. Rat Alpha2 delta-1 mutMIDAS pMT2

    Plasmid

    #58730

    Purpose

    expression of rat alpha2 delta-1 with MIDAS site mutations in mammalian cells

    Depositor

    Annette Dolphin

    Article

    Hoppa et al Nature. 2012 May 13;486(7401):122-5. doi

    Insert

    alpha2 delta-1 (Cacna2d1 Rat)
    

    Expression

    Mammalian

    Mutation

    MIDAS site mutations (D259VSGS to AVAGA)

    Promoter

    SV40

    Available Since

    Oct. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. GFP-Drosha1-850

    Plasmid

    #62522

    Purpose

    C-terminal aa851-1374 of Drosha is deleted

    Depositor

    Bharat Ramratnam

    Article

    Tang et al Nucleic Acids Res. 2010 Oct;38(19):6610-

    Insert

    human Drosha with aa 851-1374 deleted (DROSHA Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    deleted amino acids 851-1374

    Available Since

    March 20, 2015

    Availability

    Academic Institutions and Nonprofits only

16. pMT-zic2a-cytoBirA-2A-mCherry_Ras

    Plasmid

    #80064

    Purpose

    Zic2a promoter driving HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences

    Depositor

    Tatjana Sauka-Spengler

    Article

    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

    Insert

    Biotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein
    

    Use

    Zebrafish biotagging

    Tags

    3x HA

    Expression

    Bacterial

    Promoter

    Zic2a enhancer driving c-fos promoter

    Available Since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCin4-3xFLAG-MKL1 S449A/T450A/S454A

    Plasmid

    #19845

    Depositor

    Ron Prywes

    Article

    Muehlich et al Mol Cell Biol. 2008 Oct;28(20):6302-13.

    Insert

    MKL1 S449A/T450A/S454A (MRTFA Human)
    

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    changed Serine 449, Threonine 450 and Serine 454 …

    Available Since

    Dec. 16, 2008

    Availability

    Academic Institutions and Nonprofits only

18. mKO-EB3-7

    Plasmid

    #57838

    Purpose

    Localization: MT End Binding Protein, Excitation: 548, Emission: 561

    Depositor

    Michael Davidson

    Article

    Nishimura et al PLoS One. 2012;7(7):e41413. doi: 10.1371

    Insert

    EB3 (MAPRE3 Human)
    

    Tags

    mKO

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 24, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRK5 beta4 1355T

    Plasmid

    #16038

    Depositor

    Filippo Giancotti

    Article

    Nikolopoulos et al Mol Cell Biol. 2005 Jul . 25(14):6090-10

    Insert

    beta4 (ITGB4 Human)
    

    Expression

    Mammalian

    Mutation

    1355T: amino acids 1-1355

    Available Since

    Nov. 2, 2007

    Availability

    Academic Institutions and Nonprofits only

20. pLCHKO_PDPR_exon_deletion_1

    Plasmid

    #155065

    Purpose

    Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of PDPR exon using SpCas9 and LbCas12a nucleases (CHyMErA system)

    Depositor

    Jason Moffat, Benjamin Blencowe

    Article

    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

    Insert

    (hg)RNA for deletion of PDPR exon using SpCas9 and LbCas12a nucleases
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    July 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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