We narrowed to 12,286 results for: LEA

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pLenti-multi-CRISPR-sgRipk3_#1-puro

Plasmid

#231981

Purpose

Knockout mouse Ripk3

Depositor

Brian Ruffell

Publication

Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

Insert

sgRNA with Cas9 with puromycin resistance (Ripk3 Mouse)

Use

CRISPR and Lentiviral

Available since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

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AP2u2-CMV-S_protein

Plasmid

#226456

Purpose

Mammalian expression of S protein of SARS-CoV2 under CMV promoter, tagged with 6His and released extracellular environment after expression for protein purification

Depositor

Urartu Seker

Publication

Ozcelik et al ACS Pharmacol Transl Sci. 2024 Mar 21;7(

Insert

Spike Protein

Tags

6xHis Tag, Foldon Domain, and Thrombin Cleavage S…

Expression

Mammalian

Available since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pFastBacI-KDAC8 D101E

Plasmid

#224350

Purpose

Expresses human KDAC8 (HDAC8) D101E in insect cells

Depositor

Terry Watt

Publication

Toro et al Biochemistry. 2021 Aug 24;60(33):2524-25

Insert

KDAC8 (HDAC8 Human)

Tags

TEV-cleavable His6

Expression

Insect

Mutation

Aspartate 101 mutated to glutamate

Promoter

Polyhedrin

Available since

Oct. 22, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pTY152 EGFP-T2A-ORF6 SARS-CoV-1

Plasmid

#204974

Purpose

Co-express EGFP and SARS-CoV-1 ORF6

Depositor

Tim Mitchison

Publication

Yoo et al Proc Natl Acad Sci U S A. 2024 Jan 23;12

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Open Reading Frame 6 (ORF6 SARS-CoV-1)

Expression

Mammalian

Promoter

CMV

Available since

March 21, 2024

Availability

Academic Institutions and Nonprofits only

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pTY153 EGFP-T2A-ORF6 SARS-CoV-2

Plasmid

#204975

Purpose

Co-express EGFP and SARS-CoV-2 ORF6

Depositor

Tim Mitchison

Publication

Yoo et al Proc Natl Acad Sci U S A. 2024 Jan 23;12

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Open Reading Frame 6 (ORF6 SARS-CoV-2)

Expression

Mammalian

Promoter

CMV

Available since

March 21, 2024

Availability

Academic Institutions and Nonprofits only

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peGFP-N1-Cofilin-S41A

Plasmid

#186749

Purpose

Expresses S41A phosphodead GFP-tagged human cofilin in mammalian cells

Depositor

Geert van den Bogaart

Publication

Warner et al Cell Rep. 2024 Feb 27;43(3):113866. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Cofilin-1 (CFL1 Human)

Tags

GFP

Expression

Mammalian

Mutation

Changed serine 41 to alanine

Promoter

CMV

Available since

March 4, 2024

Availability

Academic Institutions and Nonprofits only

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pACS125

Plasmid

#204593

Purpose

GST-lamin A C522A (389-646)

Depositor

Tom Misteli

Publication

Schibler et al Elife. 2023 May 23;12:e80653. doi: 10.75

Insert

human lamin A C522A (389-646) (LMNA Human)

Tags

GST - lamin A C522A (389-646)

Expression

Bacterial

Available since

Jan. 11, 2024

Availability

Academic Institutions and Nonprofits only

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PET-SpCas9-HF1-plus-NLS-6xHis

Plasmid

#207381

Purpose

Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

Depositor

Ervin Welker

Publication

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

SpCas9-HF1-plus

Use

CRISPR

Tags

6xHis

Expression

Bacterial

Mutation

N497A, Q695A, Q926A, amino acids 1005-1013 replac…

Promoter

T7

Available since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-lox-inv[Talpha1-iCre-pA]-lox-mNeonGreen-hCentrin1

Plasmid

#196890

Purpose

Neuron-specific expression of human (h)Centrin fused to mNeonGreen

Depositor

Frank Bradke

Publication

Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

Insert

mNeonGreen-hCentrin1 (CETN1 Human)

Use

Cre/Lox

Expression

Mammalian

Promoter

Quimeric CAG

Available since

Sept. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pTRIPZ-Myr-PTEN

Plasmid

#206983

Purpose

Lentiviral vector for expressing doxycyclin-inducible cDNA of PTEN with myristyolation signal

Depositor

Ming Chen

Publication

Loh et al Metabolites. 2023 Aug 11;13(8):939. doi:

Insert

PTEN (PTEN Human)

Use

Lentiviral

Tags

N-terminal myristoylation site

Expression

Mammalian

Promoter

tet-on promoter

Available since

Sept. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pET28-His8-aCat(660-906)

Plasmid

#203333

Purpose

Bacterial expression of alpha-catenin F-actin binding domain

Depositor

Tina Izard

Publication

Rangarajan et al J Biol Chem. 2023 Feb;299(2):102817. doi

Insert

CTNNA1 (CTNNA1 Human)

Tags

8xHis with precision protease cleavage site

Expression

Bacterial

Mutation

aa 660-906 only

Available since

Aug. 21, 2023

Availability

Academic Institutions and Nonprofits only

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ADGRL3-CTF-Flag

Plasmid

#201219

Purpose

pCDNA3.1 with kozak (GCC), ADGRL3 C-terminal fragment and C-terminal Flag

Depositor

Jonathan Javitch

Publication

Perry-Hauser et al J Biol Chem. 2022 Dec;298(12):102594. do

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

ADGRL3 (Adgrl3 Mouse)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available since

May 9, 2023

Availability

Academic Institutions and Nonprofits only

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pX459-dCTNNA1

Plasmid

#176032

Purpose

A knock-out vector for dog CTNNA1 (alpha-1-catenin)

Depositor

Michiyuki Matsuda

Publication

Hino et al Dev Cell. 2022 Oct 10;57(19):2290-2304.e

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

A gRNA targeting the dog CTNNA1 (alpha-1-catenin) gene and the cDNA of Cas9 (CTNNA1 )

Use

CRISPR

Expression

Mammalian

Available since

Oct. 27, 2022

Availability

Academic Institutions and Nonprofits only

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human KANK2 Ankyrin Repeats pET151

Plasmid

#164054

Purpose

Expresses human KANK2 Ank domain in bacterial cells

Depositor

Ben Goult

Publication

Khan et al bioRxiv 2026.06.09.731086

Insert

Kidney Ankyrin Repeat-Containing Protein 2 (KANK2 Human)

Tags

His-tag, TEV cleavage site

Expression

Bacterial

Promoter

T7

Available since

Oct. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pCEP4-TAPBPR-TM

Plasmid

#178645

Purpose

Mammalian expression of FLAG-tagged TAPBPR with MHC TM

Depositor

Erik Procko

Publication

McShan et al Nat Chem Biol. 2022 Jun 20. pii: 10.1038

Insert

TAPBPR (TAPBPL Human)

Tags

FLAG and Signal/leader sequence from HLA class I …

Expression

Mammalian

Mutation

switched transmembrane domain with that of MHC-I

Promoter

CMV

Available since

July 11, 2022

Availability

Academic Institutions and Nonprofits only

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TVBB C-term-Dendra2-Puro

Plasmid

#169217

Purpose

Targeting vector backbone to support a knock-in of Linker-Dendra2-P2A-Puro at the C-terminus of a target locus

Depositor

Hugo Snippert

Publication

Bollen et al PLoS Biol. 2022 Jan 28;20(1):e3001527. d

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Double SapI flanked Dendra2-P2A-Puro

Use

Targeting vector backbone

Available since

Jan. 3, 2022

Availability

Academic Institutions and Nonprofits only

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Venus-7aa-MAO-pEGFP-C3

Plasmid

#166765

Purpose

To target Venus to the mitochondrial outer membrane. MAO tail anchor inserts into the outer membrane and Venus faces the cytosol. Serves as a collisional control in FLIM-FRET experiments.

Depositor

David Andrews

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

MAO tail anchor (MAOA Human)

Tags

Venus

Expression

Mammalian

Promoter

CMV

Available since

Oct. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-FLAG-PGRMC1-1-170

Plasmid

#173467

Purpose

PGRMC1 lacking the extreme C-terminus

Depositor

Bill Tsai

Publication

Chen et al Nat Commun. 2021 Oct 13;12(1):5991. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

PGRMC1 (PGRMC1 Human)

Tags

FLAG

Expression

Mammalian

Mutation

Deleted for amino acids 171-194

Available since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pHAGE2-EF1a-MCS-hPOT1_K90E_IRES-blast

Plasmid

#166412

Purpose

express hPOT1 K90E in mammalian cells

Depositor

Agnel Sfeir

Publication

Pinzaru et al Genes Dev. 2020 Dec 1;34(23-24):1619-163

Insert

hPOT1 K90E (POT1 Human)

Use

Lentiviral

Tags

MYC

Expression

Mammalian

Mutation

K90E

Promoter

EF-1a

Available since

May 6, 2021

Availability

Academic Institutions and Nonprofits only

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t206-405

Plasmid

#166132

Purpose

For expression of human talin-1 head fragment (residues 206-405) in E. coli. N-terminal His6-tag, Xpress-epitope (DLYDDDDK) and enterokinase cleavage site for tag removal.

Depositor

Vesa Hytönen

Publication

Kukkurainen et al J Cell Sci. 2020 Oct 12;133(19). pii: 13

Insert

T1head1-F2F3(206-405) (TLN1 Human)

Tags

His6-tag, Xpress-epitope (DLYDDDDK) and enterokin…

Expression

Bacterial

Mutation

residues 206-405 only

Available since

April 23, 2021

Availability

Academic Institutions and Nonprofits only

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