We narrowed to 166,390 results for: addgene

1. pX330-CENPA

   Plasmid

   #227282

   Purpose

   Expresses SpCas9 and a sgRNA targeting the N-terminus of CENPA for knock-in.

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   sgRNA Targeting N-terminus of CENPA (CENPA Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Nov. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. CCT8_pCSdest

   Plasmid

   #53795

   Depositor

   Roger Reeves

   Article

   Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

   Insert

   CCT8 (CCT8 Human)
   

   Expression

   Mammalian

   Promoter

   SP6 and CMV

   Available Since

   Sept. 9, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

3. pW267-lenti-spsgRNA-hsCDH1-sg1-pEF1s-NLS-mScarlet-I-P2A-BlastR

   Plasmid

   #170816

   Purpose

   Lentiviral vector to co-express a human CDH1 spsgRNA (sg1-Cdh1) with NLS-mScarlet-I

   Depositor

   Kenneth Yamada

   Article

   Wang et al Cell. 2021 Jul 8;184(14):3702-3716.e30.

   Insert

   NLS-mScarlet-I-P2A-BlastR
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   NA

   Available Since

   June 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. mouse CENP-B-EGFP

   Plasmid

   #107264

   Purpose

   mouse CENP-B-EGFP in pIVT vector for in vitro transcription

   Depositor

   Michael Lampson

   Article

   Akera et al Science. 2017 Nov 3;358(6363):668-672. d

   Insert

   CENP-B-EGFP (Cenpb Mouse)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   T7

   Available Since

   Sept. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLentiPGK Puro DEST JNKKTRmCerulean3

   Plasmid

   #90232

   Purpose

   Lentiviral vector to express JNK KTR mCerulean3 under PGK promoter (With Blasticidin Resistance)

   Depositor

   Markus Covert

   Article

   Kudo et al Nat Protoc. 2018 Jan;13(1):155-169. doi:

   Insert

   JNK Kinase Translocation Reporter
   

   Use

   Lentiviral

   Tags

   mCerulean3

   Expression

   Mammalian

   Promoter

   PGK

   Available Since

   March 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. qTAG-N-Solo-mStayGold-TJP1

   Plasmid

   #227300

   Purpose

   Donor template for mStayGold insertion into the N-terminus of the TJP1 locus. For tight junction visualization. To be co-transfected with sgRNA plasmid px330-TJP1 (Addgene #227299)

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   TJP1 Homology Arms flanking a mStayGold Tag (TJP1 Human)
   

   Use

   CRISPR; Donor template

   Expression

   Mammalian

   Promoter

   Promoterless

   Available Since

   Nov. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pST1374-N-NLS-flag-linker-Cas9-D10A

   Plasmid

   #51130

   Purpose

   Cas9 nickase optimized for nuclear import.

   Depositor

   Xingxu Huang

   Article

   Shen et al Nat Methods. 2014 Mar 2. doi: 10.1038/nm

   Insert

   Cas9 nickase
   

   Use

   CRISPR

   Tags

   Flag and NLS

   Expression

   Mammalian

   Mutation

   D10A (nickase mutant)

   Promoter

   CMV

   Available Since

   Feb. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pOPS0381

   Plasmid

   #133231

   Purpose

   Reporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present.

   Depositor

   Philip Poole

   Article

   Mendoza-Suarez et al Proc Natl Acad Sci U S A. 2020 May 5;117

   Insert

   constructed with constitutive promoter PsNifH (pOGG043), sfGFP (pOGG037) and T-pharma (pOGG003)
   

   Expression

   Bacterial

   Mutation

   Domesticated for Golden Gate cloning

   Available Since

   April 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pST1374-N-NLS-flag-linker-Cas9-H840A

   Plasmid

   #51129

   Purpose

   Cas9 nickase optimized for nuclear import.

   Depositor

   Xingxu Huang

   Article

   Shen et al Nat Methods. 2014 Mar 2. doi: 10.1038/nm

   Insert

   Cas9 nickase
   

   Use

   CRISPR

   Tags

   Flag and NLS

   Expression

   Mammalian

   Mutation

   H840A

   Promoter

   CMV

   Available Since

   March 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAV-CAG-oScarlet-WPRE-BC1-pA

    Plasmid

    #220765

    Purpose

    Expression of oScarlet for cell isolation and Projection-TAG BC for multiplex projection tracing

    Depositor

    Vijay Samineni

    Article

    Yang et al Nat Commun. 2025 Jul 1;16(1):5557. doi:

    Insert

    oScarlert-WPRE-BC1
    

    Use

    AAV

    Promoter

    CAG

    Available Since

    Oct. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAV-CAG-oScarlet-WPRE-BC2-pA

    Plasmid

    #220766

    Purpose

    Expression of oScarlet for cell isolation and Projection-TAG BC for multiplex projection tracing

    Depositor

    Vijay Samineni

    Article

    Yang et al Nat Commun. 2025 Jul 1;16(1):5557. doi:

    Insert

    oScarlert-WPRE-BC2
    

    Use

    AAV

    Promoter

    CAG

    Available Since

    June 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBabe puro Snail

    Plasmid

    #23347

    Depositor

    Bob Weinberg

    Article

    Mani et al Proc Natl Acad Sci U S A. 2007 Jun 12. 1

    Insert

    Snail (SNAI1 Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    Feb. 17, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMMP-2xmyc-Bcl2A1-IRES-mRFP

    Plasmid

    #36973

    Depositor

    Bill Sugden

    Article

    Pratt et al J Virol. 2012 Apr;86(8):4380-93. Epub 20

    Insert

    BCL2A1 (BCL2A1 Human)
    

    Use

    Retroviral

    Tags

    2xMYC

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 16, 2012

    Availability

    Academic Institutions and Nonprofits only

14. pSpCas9-2A-Puro-RAB7A-gRNA1 (PX459)

    Plasmid

    #221551

    Purpose

    Expresses Cas9 and gRNA for disruption of Rab7A gene in human cells

    Depositor

    Shawn Ferguson

    Article

    Talaia et al bioRxiv [Preprint]. 2023 Dec 17:2023.12.

    Insert

    gRNA targeting human Rab7A (RAB7A Human)
    

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAF123

    Plasmid

    #135921

    Purpose

    All-in-one Sleeping Beauty vector for stable transfection of eukaryotic cells with hygromycin restistance. CMV protomor drives SB100X transposase for transposon integration.

    Depositor

    Andrew S. Flies

    Article

    Flies et al Sci Adv. 2020 Jul 1;6(27). pii: 6/27/eab

    Type

    Empty backbone

    Use

    Transposon

    Expression

    Mammalian

    Available Since

    Jan. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCHC042 (∆phaCAB)

    Plasmid

    #226208

    Purpose

    Conjugation knockout plasmid (pK18msB) to delete the C. necator phaCAB operon, to prevent accumulation of PHB.

    Depositor

    Christopher Johnson

    Article

    Calvey et al Metab Eng. 2023 Jan;75:78-90. doi: 10.10

    Insert

    Homology arms for phaCAB
    

    Use

    Synthetic Biology

    Available Since

    Nov. 18, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. pBabe-puro-miR-9 sponge

    Plasmid

    #25040

    Depositor

    Bob Weinberg

    Article

    Ma et al Nat Cell Biol. 2010 Mar . 12(3):247-56.

    Insert

    miR-9 sponge
    

    Use

    Retroviral

    Tags

    d2EGFP

    Expression

    Mammalian

    Available Since

    June 11, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCas9-EcRT-GAL-Hyg

    Plasmid

    #232091

    Purpose

    Yeast CEN plasmid for galactose-inducible expression of spCas9 and Ec86 reverse transcriptase.

    Depositor

    Jeffrey Lewis

    Article

    Stuecker et al G3 (Bethesda). 2025 Aug 4:jkaf175. doi:

    Insert

    SpCas9 and Ec86 reverse transcriptase
    

    Use

    CRISPR

    Expression

    Yeast

    Mutation

    Ec86 reverse transcriptase is codon optimized for…

    Promoter

    GAL1-GAL10

    Available Since

    Feb. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pETM6-At4CL-PhCHS-CmCHI (C5 mutant, pFlavo-opt)

    Plasmid

    #73404

    Purpose

    Expresses three enzymes to convert phenylpropanoic acids to flavanones in vivo

    Depositor

    Mattheos Koffas

    Article

    Jones et al Metab Eng. 2016 Feb 6;35:55-63. doi: 10.

    Inserts

    At4CL
    PhCHS
    CmCHI
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMV-T7-AcrIIC3-NLS(SV40) (KAC209)

    Plasmid

    #134341

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized AcrIIC3 with C-terminal NLS (SV40)

    Depositor

    Joseph Bondy-Denomy, Benjamin Kleinstiver

    Article

    Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

    Insert

    human codon optimized AcrIIC3
    

    Tags

    NLS(SV40)

    Expression

    Mammalian

    Promoter

    CMV and T7

    Available Since

    March 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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