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pDEST40-2XHA-Src-KD

Plasmid

#140312

Purpose

Expresses HA-tagged human Src-KD in mammalian cells for structure-function studies

Depositor

Mark Moasser

Article

Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

Insert

Src mutant (SRC Human)

Tags

2X HA

Expression

Mammalian

Mutation

Contains only the KD region

Promoter

CMV

Available Since

April 17, 2020

Availability

Academic Institutions and Nonprofits only

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pFastBac HT JS-Munc18b

Plasmid

#135554

Purpose

Express rat Munc18b in Sf9 cells, the resulted plasmid encodes an N-terminally His6-tagged Munc18c protein with a tobacco etch virus (TEV) cleavage site between the His6 tag and Munc18b.

Depositor

Jingshi Shen

Article

Yu et al Methods Mol Biol. 2019;1860:237-249. doi

Insert

Munc18b (Stxbp2 Rat)

Tags

6xHis tag and TEV site

Expression

Insect

Promoter

polyhedrin promoter

Available Since

March 17, 2020

Availability

Academic Institutions and Nonprofits only

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mKate2-P2A-APEX2-2xML1N(7Q)

Plasmid

#67666

Purpose

Mammalian expression vector driving APEX2-ML1N (Mouse 2xML1N domain (mutant version), [marks PI(3,5)P2] fused to EM peroxidase marker). Also contains bicistronially expressed cytoplasmic red reporter.

Depositor

Rob Parton

Article

Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

Insert

mKate2-P2A-APEX2-ML1Nmut*2

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 30, 2015

Availability

Academic Institutions and Nonprofits only

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pGEX Zap70(C)-SH2

Plasmid

#46533

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Zap70(C) (ZAP70 Human)

Tags

GST and Thrombin site

Expression

Bacterial

Mutation

contains amino acids 154-267

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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ZIKV_NASBA_32B

Plasmid

#75011

Purpose

Portion of Zika Virus genome (KU312312: 7066-7399) containing sensor 32B trigger sequence and NASBA amplification sites

Depositor

James Collins, Alexander Green

Article

Pardee et al Cell. 2016 May 6. pii: S0092-8674(16)305

Insert

ZIKV_NASBA_32B

Expression

Bacterial

Promoter

T7

Available Since

April 5, 2017

Availability

Academic Institutions and Nonprofits only

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pET32a-hAREG

Plasmid

#199229

Purpose

Bacterial production of soluble, active target proteins; Nterm thrombin and enterokinase cleavage sites.

Depositor

Nilson Zanchin

Article

Ferreira et al Sci Rep. 2022 Mar 23;12(1):5034. doi: 10

Insert

Amphiregulin preproprotein (AREG Human)

Tags

TrxA-6xHis-S-tag

Expression

Bacterial

Promoter

T7

Available Since

May 10, 2023

Availability

Academic Institutions and Nonprofits only

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pNCST-AausFP4

Plasmid

#129502

Purpose

Expresses AausFP4 constitutively in E. coli (most strains)

Depositor

Nathan Shaner

Article

Lambert et al PLoS Biol. 2020 Nov 2;18(11):e3000936. d

Insert

AausFP4

Expression

Bacterial

Promoter

synthetic constitutive (stationary phase) promote…

Available Since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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ZIKV_NASBA_27B

Plasmid

#75010

Purpose

Portion of Zika Virus genome (KU312312: 1248-1594) containing sensor 27B trigger sequence and NASBA amplification sites

Depositor

James Collins, Alexander Green

Article

Pardee et al Cell. 2016 May 6. pii: S0092-8674(16)305

Insert

ZIKV_NASBA_27B

Expression

Bacterial

Promoter

T7

Available Since

April 5, 2017

Availability

Academic Institutions and Nonprofits only

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mOrange2-Keratin-17

Plasmid

#57965

Purpose

Note- This plasmid contains Keratin 18. Localization: Cytokeratin, Excitation: 549, Emission: 565

Depositor

Michael Davidson

Article

Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

Insert

Keratin (KRT18 Human)

Tags

mOrange2

Expression

Mammalian

Mutation

V282L

Promoter

CMV

Available Since

Oct. 24, 2014

Availability

Academic Institutions and Nonprofits only

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pGEX CblC-SH2

Plasmid

#46414

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Cblc CBLC (CBLC Human)

Tags

AviTag, GST, and PreScission

Expression

Bacterial

Mutation

contains amino acids 8-326

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pCS2+ oDam_f_GFPoNLS

Plasmid

#85819

Purpose

iDamID plasmid. To express transiently the optimized E. coli Dam adenine methyltransferase fused to the nuclear-localized mmGFP via flexylinker.

Depositor

Joachim Wittbrodt

Article

Gutierrez-Triana et al Development. 2016 Oct 5. pii: dev.139261

Insert

oDam_f_GFPoNLS

Tags

oNLS (optimized nuclear localization signal) C te…

Expression

Bacterial, Insect, Mammalia…

Mutation

L122A. Codon optimization compatible to work in M…

Available Since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pAc5.1/V5-HisA-RvCAHS12-GS-AcGFP1

Plasmid

#192471

Purpose

Transient expression of RvCAHS12 (fly codon optimized)-GS_linker-AcGFP1 in Drosophila cells

Depositor

Takekazu Kunieda

Article

Tanaka et al PLoS Biol. 2022 Sep 6;20(9):e3001780. do

Insert

CAHS12

Tags

AcGFP1

Expression

Insect

Promoter

Ac5

Available Since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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His-ZZ-TEV-BICDR1

Plasmid

#111859

Purpose

Full length BICDR1 for expression in Sf9 cells. 8xHis-ZZ N-terminal tag, TEV site to cleave 8x-His-ZZ.

Depositor

Andrew Carter

Article

Urnavicius et al Nature. 2018 Feb 7;554(7691):202-206. do

Insert

BICDR1 (Bicdl1 Mouse)

Tags

His-tag, TEV site, and ZZ

Expression

Insect

Available Since

July 30, 2018

Availability

Academic Institutions and Nonprofits only

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pDEST40-2XHA-Src-S4-UD

Plasmid

#140304

Purpose

Expresses HA-tagged human Src-S4-UD in mammalian cells for structure-function studies

Depositor

Mark Moasser

Article

Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

Insert

Src deletion mutant (SRC Human)

Tags

2X HA

Expression

Mammalian

Mutation

contains only the SH4 and UD regions

Promoter

CMV

Available Since

April 28, 2020

Availability

Academic Institutions and Nonprofits only

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pDEST40-2XHA-Src-S4-KD

Plasmid

#140296

Purpose

Expresses HA-tagged human Src-S4-KD in mammalian cells for structure-function studies

Depositor

Mark Moasser

Article

Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

Insert

Src deletion mutant (SRC Human)

Tags

2X HA

Expression

Mammalian

Mutation

contains only the SH4 and KD regions

Promoter

CMV

Available Since

April 28, 2020

Availability

Academic Institutions and Nonprofits only

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pDEST40-2XHA-Src-S4-UD-KD

Plasmid

#140300

Purpose

Expresses HA-tagged human Src-S4-UD-KD in mammalian cells for structure-function studies

Depositor

Mark Moasser

Article

Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

Insert

Src deletion mutant (SRC Human)

Tags

2X HA

Expression

Mammalian

Mutation

lacks the SH3 and SH2 domains

Promoter

CMV

Available Since

April 15, 2020

Availability

Academic Institutions and Nonprofits only

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STK16_HUMAN_D0

Plasmid

#79701

Purpose

This plasmid encodes the kinase domain of STK16. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

STK16 (STK16 Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
pGEX Grb7-SH2

Plasmid

#46444

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Grb7 (GRB7 Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 417-532

Promoter

Tac

Available Since

July 12, 2013

Availability

Academic Institutions and Nonprofits only

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pGEX Blk-SH2

Plasmid

#46407

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Blk (BLK Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 119-224

Promoter

Tac

Available Since

July 12, 2013

Availability

Academic Institutions and Nonprofits only

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pGEX p85b(N)-SH2

Plasmid

#46467

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

p85b(N) (PIK3R2 Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 318-431

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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