We narrowed to 14,250 results for: FLU;

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pFX-EGFP-Rab7

Plasmid

#174457

Purpose

Express EGFP with late endosome-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

EGFP with late endosome-targeting sequence

Tags

small GTPase (Rab7)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pWL502-crRNA

Plasmid

#174383

Purpose

The plasmids carrying a mini-CRISPR array were used to express crRNAs; article demonstrates use in Haloferax mediterranei

Depositor

Jing Han

Article

Lin et al Commun Biol. 2021 Aug 25;4(1):1007. doi:

Insert

a crRNA targeting the template strand

Use

CRISPR; Archaeal expression

Expression

Bacterial

Promoter

PhaR promoter

Available Since

Nov. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pFX-mCherry-Rab11

Plasmid

#174461

Purpose

Express mCherry with recycling endosome-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

mCherry with recycling endosome-targeting sequence

Tags

small GTPase (Rab11)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pMuLE_ENTR_PTCH1-mTurquoise2_R4-R3

Plasmid

#113864

Purpose

Single pathway reporter; hedgehog promoter-driven expression of mTurquoise2; MultiSite Gateway entry vector.

Depositor

Manfred Ogris

Article

Maier et al PLoS One. 2019 Dec 20;14(12):e0226570. d

Inserts

PTCH1 (hedgehog dependent promoter)
mTurquoise2

Use

Mule gateway entry vector

Expression

Mammalian

Available Since

Jan. 22, 2020

Availability

Academic Institutions and Nonprofits only

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mTagBFP2-SiT-N-15

Plasmid

#55325

Purpose

Localization: Golgi TGN, Excitation: 399, Emission: 456

Depositor

Michael Davidson

Article

Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

Insert

SiT (ST6GAL1 Human)

Tags

mTagBFP2

Expression

Mammalian

Mutation

aa 1-45 of SiT

Promoter

CMV

Available Since

Oct. 24, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pFX-mito-EGFP

Plasmid

#159096

Purpose

Express EGFP with mitochondria-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

mCherry with mitochondria-targeting sequence

Tags

the mitochondria-targeting sequence of the cytoch…

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pFX-Tom20-ｍCherry

Plasmid

#174188

Purpose

Express mCherry with mitochondria-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

mCherry with mitchondria-targeting sequence

Tags

the mitochondria-targeting sequence of TOM20

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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PCS2-FlipGFP(Casp3 cleavage seq)

Plasmid

#124432

Purpose

Expresses FlipGFP (caspase-3 cleavage sequence) protease reporter in zebrafish (verified)

Depositor

Xiaokun Shu

Article

Zhang et al J Am Chem Soc. 2019 Mar 1. doi: 10.1021/

Insert

FlipGFP(Casp3 cleavage seq)

Use

Zebrafish (good expression)

Available Since

April 18, 2019

Availability

Academic Institutions and Nonprofits only

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mRFP-FKBP12

Plasmid

#67514

Purpose

FKBP12 only

Depositor

Tamas Balla

Article

Varnai et al J Cell Biol. 2006 Nov 6;175(3):377-82.

Insert

FKBP12 (FKBP1A Human)

Tags

mRFP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 9, 2015

Availability

Academic Institutions and Nonprofits only

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pDAS12489_PEAR-GFP_2in1_2.0

Plasmid

#177186

Purpose

PEAR-GFP3 plasmid with a pegRNA that targets its own plasmid

Depositor

Ervin Welker

Article

Simon et al Elife. 2022 Feb 23;11. pii: 69504. doi:

Inserts

EGFP split with an intron between amino acids 95-96
pegRNA targeting its own plasmid

Use

CRISPR

Expression

Mammalian

Mutation

disrupted 5' splice site

Promoter

CMV and U6

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAV-U6+27-Tornado-F30-Squash-Broccoli

Plasmid

#177918

Purpose

Expresses a bifunctional circular RNA with Squash in one arm and Broccoli in the other arm of the F30 scaffold, in mammalian cells, using the Tornado expression system.

Depositor

Samie Jaffrey

Article

Dey et al Nat Chem Biol. 2021 Dec 22. pii: 10.1038

Insert

Tornado-F30-Squash-Broccoli

Expression

Mammalian

Promoter

U6+27

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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mBaoJin-lifeactin

Plasmid

#229608

Purpose

Expresses mBaoJin-lifeactin in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2024 Apr;21(4):657-665. doi

Insert

mBaoJin

Expression

Mammalian

Available Since

June 26, 2025

Availability

Academic Institutions and Nonprofits only

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pFX-mCherry-Rab7

Plasmid

#174458

Purpose

Express mCherry with late endosome-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

mCherry with late endosome-targeting sequence

Tags

small GTPase (Rab7)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pIRESneo-EGFP-alpha Tubulin

Plasmid

#12298

Depositor

Patricia Wadsworth

Article

Rusan et al Mol Biol Cell. 2001 Apr . 12(4):971-80.

Insert

EGFP-alpha Tubulin (TUBA1B Human)

Tags

EGFP

Expression

Mammalian

Available Since

Aug. 11, 2006

Availability

Academic Institutions and Nonprofits only

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pAG654 pFAST

Plasmid

#172861

Purpose

Expresses pFAST in mammalian cells

Depositor

Arnaud Gautier

Article

Benaissa et al Nat Commun. 2021 Nov 30;12(1):6989. doi:

Insert

pFAST

Tags

Myc tag (C terminal on insert)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV.CAGFLEX.(cyto).iATPSnFR2.A95A.A119L.HaloTag

Plasmid

#209657

Purpose

Expression of iATPSnFR2, low affinity

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

Insert

iATPSnFR2.A95A.A119L.HaloTag

Use

AAV

Mutation

A95A.A119L

Promoter

CAG

Available Since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-RAB-EKARev

Plasmid

#118474

Purpose

ddRFP-based single-color biosensor for monitoring ERK activity.

Depositor

Jin Zhang

Article

Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

Insert

RAB-EKARev

Tags

6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…

Expression

Mammalian

Promoter

CMV

Available Since

April 30, 2019

Availability

Academic Institutions and Nonprofits only

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FAB(ER-mito)

Plasmid

#214427

Purpose

Detection of ER-mito contact site

Depositor

Chi-Lun Chang

Article

Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

Insert

FRB-V5

Tags

CFAST10-Halo and RspAN-FRB-V5

Expression

Mammalian

Promoter

CMV

Available Since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG.(cyto).iATPSnFR2.S29W.A95K.HaloTag

Plasmid

#209651

Purpose

Expression of iATPSnFR2, high affinity

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

Insert

iATPSnFR2.S29W.HaloTag

Use

AAV

Mutation

S29W.A95K

Promoter

CAG

Available Since

June 3, 2024

Availability

Academic Institutions and Nonprofits only

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pAV-U6+27-Tornado-F30-Broccoli-Squash-SAM-sensor 5-1

Plasmid

#177920

Purpose

Expresses ratiometric Squash-SAM sensor 5-1 as circular RNA in mammalian cells using the Tornado expression system.

Depositor

Samie Jaffrey

Article

Dey et al Nat Chem Biol. 2021 Dec 22. pii: 10.1038

Insert

Tornado-F30-Broccoli-Squash-SAM-sensor 5-1

Expression

Mammalian

Promoter

U6+27

Available Since

Feb. 15, 2022

Availability

Academic Institutions and Nonprofits only

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