We narrowed to 16,256 results for: LEA

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pLVH-EF1a-GFP-P2A-KLF4

Plasmid

#130694

Purpose

Lentiviral plasmids encoding human KLF4 with co-expressoin of GFP mediated by P2A.

Depositor

Kejin Hu

Article

Shao et al Nat Commun. 2016 Mar 7;7:10869. doi: 10.

Insert

human KLF4 (KLF4 Human)

Use

Lentiviral

Available Since

Aug. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pINDUCER-21-RUNX1-P2A-ERG

Plasmid

#97045

Purpose

Expresses RUNX1-P2A-ERG in mammalian cells. *Please see notes below on P2A cleaving efficiency.

Depositor

George Daley

Article

Sugimura et al Nature. 2017 May 17. doi: 10.1038/nature

Insert

Runt Related Transcription Factor 1, ETS-Related Gene

Use

Lentiviral

Expression

Mammalian

Promoter

Tet on

Available Since

Sept. 14, 2017

Availability

Academic Institutions and Nonprofits only

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MSP2440

Plasmid

#72250

Purpose

Human expression plasmid for SpCas9-VQR-HF1 variant: CMV-T7-humanSpCas9-VQR-HF1(N497A, R661A, Q695A, Q926A, D1135V, R1335Q, T1337R)-NLS-3xFLAG

Depositor

Keith Joung

Article

Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1

Insert

mammalian codon-optimized Streptococcus pyogenes Cas9 VQR-HF1(N497A/R661A/Q695A/Q926A/D1135V/R1335Q/T1337R)-NLS-3xFlag

Use

CRISPR

Tags

3x FLAG and NLS

Expression

Mammalian

Mutation

N497A, R661A, Q695A, Q926A, D1135V, R1335Q, and T…

Promoter

CMV

Available Since

Jan. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT/TO-3*Flag-APEX2 N-term WRAP53

Plasmid

#187578

Purpose

Express FLAG epitope and APEX2-tagged WRAP53 fusion protein in mammalian cells

Depositor

Benjamin Blencowe

Article

Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

Insert

WRAP53 (WRAP53 Human)

Tags

APEX2-FLAG

Expression

Mammalian

Promoter

CMV

Available Since

July 26, 2022

Availability

Academic Institutions and Nonprofits only

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proISG15 (2-165)

Plasmid

#110762

Purpose

Bacterial expression of proISG15

Depositor

David Komander

Article

Swatek et al Proc Natl Acad Sci U S A. 2018 Mar 6;115

Insert

ISG15 (ISG15 Human)

Tags

N-His6-3C

Expression

Bacterial

Promoter

T7

Available Since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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TVBB C-term-mNeongreen

Plasmid

#169227

Purpose

Targeting vector backbone to support a knock-in of Linker-mNeongreen at the C-terminus of a target locus

Depositor

Hugo Snippert

Article

Bollen et al PLoS Biol. 2022 Jan 28;20(1):e3001527. d

Insert

Double SapI flanked mNeongreen

Use

Targeting vector backbone

Available Since

Jan. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pHis-LMW_FGF2

Plasmid

#157657

Purpose

Expresses FGF2 in E.coli cell

Depositor

Byung Il Lee

Article

Bong et al Nucleic Acids Res. 2020 Jun 19;48(11):63

Insert

Fibroblast growth factor 2 (FGF2 Human)

Tags

His tag

Expression

Bacterial

Mutation

changed Cystein 211,229 to serine, deleted amino …

Available Since

Aug. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn1-GRAB-HTR6-cilia

Plasmid

#189615

Purpose

Expresses cilia-targeted serotonin sensor based on HTR6 receptor and cpEGFP, driven by SYN promoter

Depositor

David Clapham

Article

Sheu et al Cell. 2022 Sep 1;185(18):3390-3407.e18.

Insert

HTR6_GRAB

Use

AAV

Promoter

Syn1

Available Since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV_UdgX-APOBEC1-UdgX-NG-nCas9

Plasmid

#163562

Purpose

Mammalian CG-to-GC base editing

Depositor

David Liu

Article

Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

Insert

UdgX-APOBEC1-UdgX-NG-nCas9

Use

CRISPR

Expression

Mammalian

Mutation

nCas9 (D10A)NG (L111R, D1135V, G1218R, E1219F, A1…

Available Since

Dec. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1a-EGFP-TA MAO-IRES-Puromycin

Plasmid

#133025

Purpose

Expresses EGFP-tagged tail anchor sequence of monoamine oxidase A

Depositor

David Andrews

Article

Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

Monoamine oxidase type A (MAOA Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

deleted amino acids 1-488

Promoter

Human elongation factor 1 alpha

Available Since

Feb. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV_UdgX-Anc689-UdgX-NG-nCas9-RBMX

Plasmid

#163554

Purpose

Mammalian CG-to-GC base editing

Depositor

David Liu

Article

Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

Insert

UdgX-Anc689-UdgX-NG-nCas9-RBMX

Use

CRISPR

Expression

Mammalian

Mutation

nCas9 (D10A)NG (L111R, D1135V, G1218R, E1219F, A1…

Available Since

Dec. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn Con/Foff EYFP

Plasmid

#55651

Purpose

Cre-on/Flp-off EYFP under the Synapsin promoter

Depositor

Karl Deisseroth

Article

Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

Insert

eYFP with introns

Use

AAV; Cre on/flp off eyfp

Expression

Mammalian

Promoter

hSyn

Available Since

Aug. 11, 2014

Availability

Academic Institutions and Nonprofits only

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EF1a_DIO_mMeCP2-HaloTag

Plasmid

#164062

Purpose

Double-Floxed Inverted Open reading frame for mouseMeCP2(alpha) Under EF1a promoter

Depositor

Nathaniel Heintz

Article

Piccolo et al Elife. 2019 Dec 23;8. pii: 51449. doi: 1

Insert

MeCP2 Isoform 1 (Mecp2 Mouse)

Use

AAV

Tags

HaloTag

Expression

Mammalian

Promoter

EF1a

Available Since

Feb. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-MCP-SRSF3-V5-mCherry

Plasmid

#235090

Purpose

SRSF3 mCherry fusion protein with MCP domain

Depositor

Mitchell Guttman

Article

Bhat et al Nature. 2024 May;629(8014):1165-1173. do

Insert

pCAG-MCP-SRSF3-V5-mCherry (SRSF3 Human)

Expression

Mammalian

Promoter

CAG

Available Since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

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pMIG-hNFATc1/bC

Plasmid

#74049

Purpose

retroviral expression plasmid for human NFATc1/bC

Depositor

Ria Baumgrass

Article

Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

Insert

human NFATc1, isoform beta-C (NFATC1 Human)

Use

Retroviral

Tags

no tag

Expression

Mammalian

Promoter

pMSCV-LTRs

Available Since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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pBK092

Plasmid

#183146

Purpose

pET-6xHis-MBP-TEV-CrtTIR-APAZ/CrtAgo - Heterologous CrtSPARTA expression

Depositor

Daan Swarts

Article

Koopal et al Cell. 2022 Apr 28;185(9):1471-1486.e19.

Insert

CrtSPARTA operon (6xHis-MBP-CrtTIR-APAZ and CrtAgo)

Tags

6xHis and MBP

Expression

Bacterial

Promoter

T7

Available Since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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pGP-CMV-GCaMP6-210

Plasmid

#91778

Purpose

Mammalian expression of low affinity calcium-sensing fluorescent protein

Depositor

Douglas Kim, GENIE Project

Article

de Juan-Sanz et al Neuron. 2017 Jan 30. pii: S0896-6273(17)

Insert

GCaMP6-210

Tags

6xHis, T7 epitope, and Xpress tag

Expression

Mammalian

Promoter

CMV

Available Since

June 27, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV_eA3A T31A-NG-nCas9

Plasmid

#163539

Purpose

Mammalian CG-to-GC base editing

Depositor

David Liu

Article

Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

Insert

eA3A T31A-NG-nCas9

Use

CRISPR

Expression

Mammalian

Mutation

nCas9 (D10A)eA3A T31ANG (L111R, D1135V, G1218R, E…

Available Since

Dec. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV_UdgX-APOBEC1-UdgX-HF-NG-nCas9

Plasmid

#163561

Purpose

Mammalian CG-to-GC base editing

Depositor

David Liu

Article

Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

Insert

UdgX-APOBEC1-UdgX-HF-NG-nCas9

Use

CRISPR

Expression

Mammalian

Mutation

nCas9 (D10A)N497A, R661A, Q695A, Q926A//L111R, D1…

Available Since

Jan. 4, 2021

Availability

Academic Institutions and Nonprofits only

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SHMT2-RES

Plasmid

#72880

Purpose

overexpression

Depositor

David Sabatini

Article

Kim et al Nature. 2015 Apr 16;520(7547):363-7. doi

Insert

SHMT2 (SHMT2 Human)

Use

Retroviral

Expression

Mammalian

Mutation

silent mutations in site corresponding to shSHMT2…

Available Since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only