We narrowed to 9,499 results for: pho;

1. pRSFDuet-1 IntS11 AA 300-597

   Plasmid

   #199329

   Purpose

   Expresses His-tagged Drosophila IntS11 amino acids 300-597

   Depositor

   Jeremy Wilusz

   Article

   Fujiwara et al Mol Cell. 2023 Nov 14:S1097-2765(23)0090

   Insert

   Integrator subunit 11 (IntS11 Fly)
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   amino acids 300-597 only

   Available Since

   Nov. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. LENTICRISPR-TPI1_sgRNA1

   Plasmid

   #201626

   Purpose

   CRISPR/Cas9-mediated gene knock-out

   Depositor

   Alexis Jourdain, Vamsi Mootha

   Article

   Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

   Insert

   TPI1 (TPI1 Human)
   

   Use

   CRISPR and Lentiviral

   Available Since

   June 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. LENTICRISPR-TPI1_sgRNA2

   Plasmid

   #201627

   Purpose

   CRISPR/Cas9-mediated gene knock-out

   Depositor

   Alexis Jourdain, Vamsi Mootha

   Article

   Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

   Insert

   TPI1 (TPI1 Human)
   

   Use

   CRISPR and Lentiviral

   Available Since

   June 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMX-SHP1C-ires-hCD8

   Plasmid

   #183720

   Purpose

   Expresses the myc-tagged catalytic domains of wild-type SHP-1.

   Depositor

   Takeshi Tsubata

   Article

   Alborzian Deh Sheikh et al J Immunol. 2021 Jun 1;206(11):2544-2551.

   Insert

   SHP-1
   

   Use

   Retroviral

   Mutation

   catalitic domain alone

   Available Since

   July 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. YFP-Stim1-ΔCTD

   Plasmid

   #186621

   Purpose

   Encodes Stim1 with C-terminal domain deletion fused to YFP

   Depositor

   Gia Voeltz

   Article

   Zamponi et al Dev Cell. 2022 Jun 6;57(11):1369-1382.e6

   Insert

   Stromal Interaction Molecule 1 (STIM1 Human)
   

   Tags

   YFP

   Expression

   Mammalian

   Mutation

   Deletion of C-terminal domain 601 to 685

   Promoter

   CMV

   Available Since

   Feb. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. Neurod1-Atoh7HA3

   Plasmid

   #44438

   Depositor

   William Klein

   Article

   Mao et al Development. 2013 Feb;140(3):541-51. doi

   Insert

   Atoh7-3xHA knock-in (Atoh7 Mouse)
   

   Use

   Cre/Lox and Mouse Targeting

   Tags

   Neurod1 left arm, Neurod1 right arm, and loxC2-Re…

   Expression

   Mammalian

   Available Since

   July 23, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3-mCherry-TSC2(AA)-NLS

   Plasmid

   #192443

   Purpose

   Encodes mCherry-tagged TSC2 isoform 5 with S939A, T1439A mutations to block Akt phosphorylation; targeted to the nucleus.

   Depositor

   Jin Zhang

   Article

   Zhong et al Cell Chem Biol. 2022 Jun 16;29(6):1037-1

   Insert

   mCherry-TSC2(S939A,T1439A)-NLS (TSC2 Human)
   

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

   Expression

   Mammalian

   Mutation

   TSC2 Ser 939 and Thr 1439 (equivalent to 1462 in …

   Promoter

   CMV

   Available Since

   Dec. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSK+ mBMPR-1B DN

   Plasmid

   #49530

   Purpose

   cloning vector for dominant negative mouse BMP receptor 1B

   Depositor

   Lee Niswander, Peter ten Dijke

   Article

   Zou et al Genes Dev. 1997 Sep 1;11(17):2191-203.

   Insert

   BMPR-1B DN (Bmpr1b Mouse)
   

   Expression

   Bacterial

   Mutation

   dominant negative mutation (K231R); also L2V

   Promoter

   T3

   Available Since

   May 20, 2014

   Availability

   Academic Institutions and Nonprofits only

9. pAAV hSyn1 CaRhAC T2A tDimer

   Plasmid

   #101722

   Purpose

   - humanized - variant of pAAV hSyn YFP-CaRhAC Addgene # 101721– less reliable in hippocampal neurons

   Depositor

   Thomas Oertner

   Article

   Scheib et al Nat Commun. 2018 May 24;9(1):2046. doi:

   Inserts

   CatRhAC
   red fluorescent protein
   

   Use

   AAV

   Mutation

   E497K,C569D

   Promoter

   human Synapsin1 promotor and ribosomal skip seque…

   Available Since

   Jan. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA6-N-3XFLAG-Ubac2

    Plasmid

    #123597

    Depositor

    Bruce Beutler

    Article

    Choi et al Science. 2019 May 10;364(6440). pii: 364

    Insert

    ubiquitin associated domain containing 2 (Ubac2 Mouse)
    

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    July 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. tet-pLKO shMKP5.v2 puro

    Plasmid

    #87793

    Purpose

    Expresses an inducible short hairpin targeting human MKP5 sequence

    Depositor

    Kevin Janes

    Article

    Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

    Insert

    shMKP5 (DUSP10 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    TRE tight

    Available Since

    March 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. tet-pLKO shMKP5.v1 puro

    Plasmid

    #87792

    Purpose

    Expresses an inducible short hairpin targeting human MKP5 sequence

    Depositor

    Kevin Janes

    Article

    Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

    Insert

    shMKP5 (DUSP10 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    TRE tight

    Available Since

    March 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEGFP-N1-WDR34

    Plasmid

    #66797

    Purpose

    Expression of human WDR34 tagged with GFP under the control of CMV

    Depositor

    Lotte Pedersen

    Article

    Goggolidou et al Development. 2014 Oct;141(20):3966-77. d

    Insert

    WDR34 (WDR34 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 15, 2015

    Availability

    Academic Institutions and Nonprofits only

14. pSpCas9_BB_2A-GFP_MAPRE1-gRNA#2

    Plasmid

    #107727

    Purpose

    Knock out of EB1 in human cells by CRISPR/Cas9

    Depositor

    Torsten Wittmann

    Article

    van Haren et al Nat Cell Biol. 2018 Jan 29. pii: 10.1038

    Insert

    MAPRE1 gRNA #1 (targets Exon 1) (MAPRE1 Human)
    

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    April 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBAD mApple

    Plasmid

    #91772

    Purpose

    Bacterial expression of a fluorescent protein, monomeric Apple (mApple). Note: Addgene identified several mutations compared to the final published sequence.

    Depositor

    Erik Rodriguez, Roger Tsien

    Article

    Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

    Insert

    monomeric Apple
    

    Tags

    Hexa-Histidine tag, Xpress epitope for detection …

    Expression

    Bacterial

    Mutation

    R97K, E152S, A180S, V207T compared to mApple (FP …

    Promoter

    araBAD

    Available Since

    June 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-hSyn-mCherry.3xFLAG.NOS1APΔC20-WPRE

    Plasmid

    #127865

    Purpose

    pAAV plasmid expressing an mCherry.3xFLAG.NOS1APΔC20 fusion protein under the hSyn promoter

    Depositor

    Florian Freudenberg

    Article

    Candemir et al Eur Neuropsychopharmacol. 2016 Apr;26(4)

    Insert

    Nos1ap (Nos1ap Mouse)
    

    Use

    AAV

    Tags

    3xFLAG and mCherry

    Mutation

    deleted the c-terminal 20 amino acids

    Promoter

    hSyn

    Available Since

    Aug. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. tet-pLKO shMKP1.v2 puro

    Plasmid

    #87791

    Purpose

    Expresses an inducible short hairpin targeting human MKP1 sequence

    Depositor

    Kevin Janes

    Article

    Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

    Insert

    shMKP1 (DUSP1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    TRE tight

    Available Since

    March 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA1-Bystin

    Plasmid

    #11869

    Depositor

    Michiko Fukuda

    Article

    Suzuki et al Proc Natl Acad Sci U S A. 1998 Apr 28. 9

    Insert

    Bystin (BYSL Human)
    

    Expression

    Mammalian

    Available Since

    Sept. 1, 2006

    Availability

    Academic Institutions and Nonprofits only

19. Sema3a(L, del13)-AP-His

    Plasmid

    #72011

    Purpose

    Expresses the Sema3A protein (truncated at cleavage site P3; ie, long and missing exon 13), C-terminally fused to alkaline phosphatase + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Sema3a (Sema3a Mouse)
    

    Tags

    AP-His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. TetO-FUW-Bmi1

    Plasmid

    #61532

    Purpose

    Expresses Bmi1 in mammalian cells

    Depositor

    Rudolf Jaenisch

    Article

    Cassady et al Stem Cell Reports. 2014 Nov 6. pii: S221

    Insert

    Bmi1 (Bmi1 Mouse)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Jan. 23, 2015

    Availability

    Academic Institutions and Nonprofits only