We narrowed to 18,339 results for: Mut

1. pmcherry α-catenin ΔVBS

   Plasmid

   #178649

   Purpose

   express α-catenin without a vinculin binding site (VBS)

   Depositor

   Daniel Conway

   Article

   Bejar-Padilla et al Mol Biol Cell. 2022 Aug 3:mbcE22020071.

   Insert

   α-catenin (CTNNA1 Human)
   

   Use

   Tags

   mcherry

   Expression

   Mammalian

   Mutation

   VBS of alpha catenin was removed and replaced wit…

   Promoter

   Available Since

   Aug. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. eSpCas9-plus

   Plasmid

   #126767

   Purpose

   Expression plasmid for human codon-optimized increased fidelity eSpCas9-plus (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with close to the same fidelity as eSpCas9.

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

   Insert

   eSpCas9-plus
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   K848A, R1060A; amino acids 1005-1013 replaced wit…

   Promoter

   CBh

   Available Since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRK5 FLAG DEPTOR (13×S/T->A)

   Plasmid

   #21702

   Depositor

   David Sabatini

   Article

   Peterson et al Cell. 2009 May 29;137(5):873-86. doi: 10

   Insert

   DEPTOR 13xA (DEPTOR Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Nonphosphorylatable mutant. 13xA. Also has N204S …

   Promoter

   Available Since

   Aug. 12, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHAGE-TRIM33__RET

   Plasmid

   #116841

   Purpose

   Lentiviral expression of TRIM33__RET fusion

   Depositor

   Kenneth Scott, Gordon Mills

   Article

   Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

   Insert

   TRIM33__RET
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   March 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pIRES-hrGFP II-mTET1

   Plasmid

   #83569

   Purpose

   Expresses TET1 with a mutated catalytic domain in mammalian cells

   Depositor

   Jean-Pierre Issa

   Article

   Jin et al Nucleic Acids Res. 2014 Jun;42(11):6956-

   Insert

   Tet Methylcytosine Dioxygenase 1 (TET1 Human)
   

   Use

   Tags

   3X FLAG tag and hr GFP II

   Expression

   Mammalian

   Mutation

   catalytic domain mutant H1672D, D1674A

   Promoter

   CMV

   Available Since

   Sept. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pACEBac1-PARP1-Flag-His-7xKQ

   Plasmid

   #111573

   Purpose

   Expresses full length PARP1 with K to Q mutations in automodification domain in Sf9 (insect) cells

   Depositor

   Thomas Muir

   Article

   Liszczak et al Nat Chem Biol. 2018 Jul 16. pii: 10.1038

   Insert

   PARP1 (PARP1 Human)
   

   Use

   Tags

   C-terminal 6xHis and C-terminal Flag

   Expression

   Insect

   Mutation

   lysine to glutamine mutation at the following ami…

   Promoter

   polyhedrin promoter

   Available Since

   July 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA6.2EmGFP-cDAXX

   Plasmid

   #169727

   Purpose

   Mammalian expression construct of GFP tagged cDAXX

   Depositor

   Tanja Mittag

   Article

   Bouchard et al Mol Cell. 2018 Oct 4;72(1):19-36.e8. doi

   Insert

   cDAXX (DAXX Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Comprises residues 495-740

   Promoter

   CMV

   Available Since

   June 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. AAVS1-EF1a-mTagRFP-T-CAAX

   Plasmid

   #167973

   Purpose

   AAVS1 targeting repair template

   Depositor

   Emma Rawlins

   Article

   Sun et al eLife. 2021 Oct 6;10. pii: 67886. doi: 1

   Insert

   AAVS1 (PPP1R12C, AAVS1 Human)
   

   Use

   CRISPR; Donor template

   Tags

   mTagRFP-T-CAAX

   Expression

   Mammalian

   Mutation

   homology arms contain point mutations to disrupt …

   Promoter

   Available Since

   June 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. Str-STIM1-NN_puromycin

   Plasmid

   #65310

   Purpose

   Luminal ER hook only (RUSH system)

   Depositor

   Franck Perez

   Article

   Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

   Insert

   STIM1 mutated for binding motif to microtubule (STIM1 Human)
   

   Use

   Tags

   streptavidin

   Expression

   Mammalian

   Mutation

   2 amino acids of STIM1 (IP from SxIP motif) were …

   Promoter

   CMV

   Available Since

   June 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. PHAGE-P CMVt N-HA hE6-AP I (AA 366-852)

    Plasmid

    #119315

    Purpose

    lentiviral vector for expression of HA-Tagged E6-AP I AA 366-852 in mammalian cells

    Depositor

    Peter Howley

    Article

    Kuhnle et al J Biol Chem. 2018 Sep 26. pii: RA118.004

    Insert

    E6-AP I (UBE3A Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mutation

    Only AA 366-852

    Promoter

    CMV

    Available Since

    March 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMSCV-IRES-hCD2-CD14 (4R)

    Plasmid

    #133062

    Purpose

    Retroviral expression of mouse CD14 mutant "4R" deficient in LPS binding

    Depositor

    Jonathan Kagan

    Article

    Tan et al Immunity. 2015 Nov 17;43(5):909-22. doi:

    Insert

    CD14 (4R) (Cd14 Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    CD14 allele defective for LPS binding (mutations …

    Promoter

    Available Since

    Oct. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEGFP-NMHC-IIA-3xA

    Plasmid

    #101041

    Purpose

    Expresses GFP-MYH9 construct (human myosin IIA) carrying S1943A mutation and S1916A, S1915A mutation, driven by CMV promoter. Thus inhibits CKII and PKC phosphorylation of the tail.

    Depositor

    Tom Egelhoff

    Article

    Rai et al J Biol Chem. 2017 Feb 24;292(8):3099-311

    Insert

    Non-muscle myosin IIA heavy chain (MYH9 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    S1915A, S1916A, S1943A

    Promoter

    CMV

    Available Since

    Nov. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. Venus-Noxa-4E-pEGFP-C1

    Plasmid

    #166745

    Purpose

    Express Venus fused to the N-terminus of the Bcl-2 family protein, Noxa with mutation in BH3 domain to disrupt binding anti-apoptotic proteins

    Depositor

    David Andrews

    Article

    Osterlund et al Sci Adv. 2022 Apr 22;8(16):eabm7375. doi

    Insert

    Noxa-4E (PMAIP1 Human)
    

    Use

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    4 hydrophobic residues in the BH3 region mutated …

    Promoter

    CMV

    Available Since

    April 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHAGE-EGFR__ERP44

    Plasmid

    #116815

    Purpose

    Lentiviral expression of EGFR__ERP44 fusion

    Depositor

    Kenneth Scott, Gordon Mills

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    EGFR__ERP44
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. lenti-HA-mBec1

    Plasmid

    #99505

    Purpose

    3rd generation lentiviral expression of wild-type Becn1

    Depositor

    Congcong He

    Article

    Rocchi et al PLoS Genet. 2017 Aug 14;13(8):e1006962.

    Insert

    Beclin 1 (Becn1 Mouse)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pYPQ230-RVR

    Plasmid

    #108861

    Purpose

    LbCpf1 Gateway entry plasmid with G548R, K554V and Y558R mutations

    Depositor

    Yiping Qi

    Article

    Zhong et al Mol Plant. 2018 Jul 2;11(7):999-1002. do

    Insert

    LbCpf1-RVR (LbCpf1 with G548R, K554V and Y558R mutations)
    

    Use

    CRISPR; Gateway compatible cpf1 entry clone

    Tags

    NLS

    Expression

    Plant

    Mutation

    G548R, K554V and Y558R, Cpf1 is rice codon optimi…

    Promoter

    Available Since

    Aug. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLPC-Progerin-CS

    Plasmid

    #69064

    Purpose

    encodes Progerin with a substitution of cysteine in CaaX-motif by a serine

    Depositor

    Gerardo Ferbeyre

    Article

    Moiseeva et al Cell Cycle. 2015 Aug 3;14(15):2408-21. d

    Insert

    Progerin (LMNA Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Progerin with substitution of cysteine in CaaX-mo…

    Promoter

    CMV

    Available Since

    Dec. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

18. pX330 Ctnnb1.2

    Plasmid

    #59912

    Purpose

    pX330 backbone expressing sgRNA targeting Ctnnb1 to edit mouse beta-Catenin. Expresses Cas9 from CBh promoter

    Depositor

    Tyler Jacks

    Article

    Xue et al Nature. 2014 Aug 6. doi: 10.1038/nature1

    Insert

    Ctnnb1 (Ctnnb1 Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHAGE-AKAP13__RET

    Plasmid

    #116806

    Purpose

    Lentiviral expression of AKAP13__RET fusion

    Depositor

    Kenneth Scott, Gordon Mills

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    AKAP13__RET
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pTol2CG NBT:NRGIIIa:polyA

    Plasmid

    #193012

    Purpose

    Tol2 transgenic construct that will express human Neuregulin1 Type IIIa in neurons

    Depositor

    William Talbot

    Article

    Lysko et al Hum Mutat. 2022 Sep;43(9):1216-1223. doi

    Inserts

    NBT
    NRG1 type IIIa (NRG1 Human)
    

    Use

    Transposon transgenesis

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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