We narrowed to 166,390 results for: addgene

1. pBEG R2-iHygro-L3 #BGV206

   Plasmid

   #48985

   Purpose

   Supplies the selection marker (hygromycin resistance) and IRES with flanked by AttR2 and AttL3 sites for Gateway cloning recombination reaction. (This corresponds to a module 3 plasmid).

   Depositor

   David Dankort

   Article

   Geiling et al PLoS One. 2013 Oct 11;8(10):e76279. doi:

   Insert

   hygromycin phosphotransferase
   

   Use

   Gateway cloning vector

   Tags

   IRES (weak)

   Promoter

   na

   Available Since

   June 16, 2014

   Availability

   Academic Institutions and Nonprofits only

2. F-CDK8L

   Plasmid

   #24762

   Depositor

   Joan Conaway, Ronald Conaway

   Article

   Conaway et al FEBS Lett. 2005 Feb 7. 579(4):904-8.

   Insert

   F-CDK8L (CDK19 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Available Since

   May 14, 2010

   Availability

   Academic Institutions and Nonprofits only

3. pW193-lenti-sasgRNA-lacZ-pEF1s-NLS-mNeonGreen-P2A-BlastR

   Plasmid

   #170814

   Purpose

   Lentiviral vector to co-express a lacZ control sasgRNA with NLS-mNeonGreen

   Depositor

   Kenneth Yamada

   Article

   Wang et al Cell. 2021 Jul 8;184(14):3702-3716.e30.

   Insert

   NLS-mNeonGreen-P2A-BlastR
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   NA

   Available Since

   June 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEBG-PELP1

   Plasmid

   #65209

   Purpose

   Full length mammalian expression vector for hPELP1

   Depositor

   Ratna Vadlamudi

   Article

   Nair et al Cancer Res. 2010 Sep 15;70(18):7166-75.

   Insert

   PELP1 (PELP1 Human)
   

   Tags

   GST

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   June 29, 2015

   Availability

   Academic Institutions and Nonprofits only

5. PX458_AHR_2

   Plasmid

   #101077

   Purpose

   Encodes gRNA for 3' target of human AHR

   Depositor

   Eric Mendenhall, Richard M. Myers

   Article

   Savic et al Genome Res. 2015 Sep 9.

   Insert

   gRNA against AHR (AHR Human)
   

   Use

   CRISPR

   Available Since

   Oct. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV-T7-AcrIIA17_Efa-NLS(SV40) (KAC91)

   Plasmid

   #134333

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized AcrIIA17_Efa with C-terminal NLS (SV40)

   Depositor

   Joseph Bondy-Denomy, Benjamin Kleinstiver

   Article

   Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

   Insert

   human codon optimized AcrIIA17_Efa
   

   Tags

   NLS(SV40)

   Expression

   Mammalian

   Promoter

   CMV and T7

   Available Since

   March 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. LLP780_SpyCatcher-KRAB-CO

   Plasmid

   #211773

   Purpose

   SpyTag counterpart binding domain, SpyCatcher, fused to transcriptional repressor KRAB, with GFP selection

   Depositor

   Ryan Lister

   Article

   Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

   Insert

   SpyCatcher-KRAB
   

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Last 300 bp codon-optimised (CO) to detect KRAB e…

   Promoter

   pEF1a and pSV40

   Available Since

   Dec. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDR348 FANCF

   Plasmid

   #47510

   Purpose

   human gRNA expression vector targeting FANCF

   Depositor

   Keith Joung

   Article

   Fu et al Nat Biotechnol. 2013 Jun 23. doi: 10.103

   Insert

   gRNA-FANCF (FANCF Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Aug. 29, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. Sufu_pLENTI-CAG-IRES-GFP

   Plasmid

   #176993

   Purpose

   Mammalian lentiviral expression vector encoding Sufu

   Depositor

   Roger Reeves

   Article

   Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

   Insert

   Sufu (Sufu Mouse)
   

   Use

   Lentiviral

   Promoter

   CAG

   Available Since

   April 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-CAG-SuperNovaGreen-T2A-dTomato

    Plasmid

    #193798

    Purpose

    Expresses SuperNova Green in the cytosol of mammalian cells, joined by a T2A cleavage site with dTomato

    Depositor

    Xiao Wang

    Article

    Sessler et al Sci Adv. 2022 Dec 9;8(49):eade1136. doi:

    Insert

    SuperNova Green
    

    Use

    AAV

    Tags

    Flag tag

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Jan. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pNH13 (pmyo-2::QuasAr::mOrange)

    Plasmid

    #130273

    Purpose

    Expresses the eFRET-based voltage sensor QuasAr::mOrange in the pharynx of C. elegans.

    Depositor

    Alexander Gottschalk

    Article

    Azimi Hashemi et al Proc Natl Acad Sci U S A. 2019 Aug 1. pi

    Insert

    QuasAr::mOrange
    

    Tags

    mOrange

    Expression

    Worm

    Available Since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCW57.1-Tet-UPF1S124A/N138A/T139A (pRKB268)

    Plasmid

    #99144

    Purpose

    Inducible lentiviral construct to express FLAG-tagged UPF1S124A/N138A/T139A

    Depositor

    Robert Bradley

    Article

    Feng et al Mol Cell. 2017 Jul 20;67(2):239-251.e6.

    Insert

    UPF1 (UPF1 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    First 127 codons are optimized to reduce GC conte…

    Promoter

    pTight

    Available Since

    Nov. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. p-mCherry2-sgMUC4

    Plasmid

    #198399

    Purpose

    Expresses a guide RNA against a repetitive locus found in the MUC4 gene of human chromosome 3, with mCherry2 reporter

    Depositor

    Sarah McClelland

    Article

    Tovini et al EMBO J. 2023 May 15;42(10):e111587. doi:

    Insert

    MUC4 sgRNA (MUC4 Human)
    

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    April 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV Syn ChR E90R-D156N-T159C 2A tDimer

    Plasmid

    #52494

    Purpose

    Historical plasmid. For inhibition of neuronal activity, note that Addgene Plasmid #66709 is a light-gated chloride channel with improved anion selectivity (iChloC)

    Depositor

    Thomas Oertner

    Article

    Wietek et al Science. 2014 Mar 27.

    Inserts

    Channelrhodopsin-2
    red fluorescent protein
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    E90R, D156N, T159C

    Promoter

    human synapsin-1 and ribosomal skip sequence

    Available Since

    April 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBV-luc TOP2A 1kb promoter

    Plasmid

    #138274

    Purpose

    Luciferase reporter plasmid driven by TOP2A promoter

    Depositor

    Wen Xue

    Article

    Kwan et al Hepatology. 2019 Jun 12. doi: 10.1002/he

    Insert

    TOP2A promoter sequence
    

    Use

    Luciferase

    Expression

    Mammalian

    Available Since

    March 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBABE-puro-mIR-31 sponge

    Plasmid

    #22694

    Purpose

    Stable suppression of miR-31 function

    Depositor

    Bob Weinberg

    Article

    Valastyan et al Cell. 2009 Jun 12. 137(6):1032-46.

    Insert

    miR-31 (MIR31 Human)
    

    Use

    Retroviral

    Tags

    *

    Expression

    Mammalian

    Mutation

    miR-31 sponge with 7x tandem binding sites to the…

    Available Since

    March 11, 2010

    Availability

    Academic Institutions and Nonprofits only

17. C2CD2_pCSdest

    Plasmid

    #53916

    Depositor

    Roger Reeves

    Article

    Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

    Insert

    C2CD2 (C2CD2 Human)
    

    Expression

    Mammalian

    Promoter

    SP6 and CMV

    Available Since

    Sept. 8, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

18. pEN_TT 3xFLAG-KPNA2

    Plasmid

    #192306

    Purpose

    Gateway entry vector for an inducible 3xFLAG-KPNA2

    Depositor

    Kevin Janes

    Article

    Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

    Insert

    KPNA2 (KPNA2 Human)
    

    Use

    Gateway entry vector

    Tags

    3xFLAG

    Available Since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLKO-puro human lipin 1-1 shRNA

    Plasmid

    #32019

    Depositor

    David Sabatini

    Article

    Peterson et al Cell. 2011 Aug 5;146(3):408-20.

    Insert

    lipin1 shRNA
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Sept. 27, 2011

    Availability

    Academic Institutions and Nonprofits only

20. pcDNA3.2 mir-1-1 reporter hsa-mir-133a-1

    Plasmid

    #46676

    Depositor

    David Bartel

    Article

    Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

    Insert

    hsa-mir-133a-1 (MIR133A1 Human)
    

    Use

    RNAi

    Tags

    V5 and hsa-mir-1-1

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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