Addgene

We narrowed to 2,771 results for: BFP

Showing: 701 - 720 of 2771 results

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pAAV_CAG_NES-Kinprola_PKA-mTagBFP2_WPRE-SV40

Plasmid

#233364

Purpose

CAG driven expression of the PKA activity recorder Kinprola_PKA fused to mTagBFP2 for mammalian cell expression through AAV transduction

Depositor

Kai Johnsson

Article

Sun et al bioRxiv 2024.09.11.611894

Insert

NES-Kinprola_PKA-mTagBFP2

Use

AAV

Tags

NES and mTagBFP2

Expression

Mutation

Promoter

CAG

Available since

March 26, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.BC(p1-10)-SCP1-TagBFP2-W3SL-BC(p1-10)

Plasmid

#231355

Purpose

Single stranded AAV genome with concatemerization-dependent barcodes, for tracking AAV concatemers via SpECTr. Also expresses TagBFP from SCP1 promoter.

Depositor

Viviana Gradinaru

Article

Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

Insert

BC(p1-10)

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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hARF6-R WT TurboID V5 T2A BFP

Plasmid

#233256

Purpose

Expression of BFP-tagged ARF6 WT for Turbo ID experiments

Depositor

David Bryant

Article

Nikolatou et al EMBO J. 2023 Sep 18;42(18):e113987. doi:

Insert

ARF6 (ARF6 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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psLIB TRE GTS-mTagBFP

Plasmid

#229969

Purpose

encodes a mTagBFP targeted to Golgi apparatus under the control of a dox inducible promoter

Depositor

Martin Pelosse

Article

Pelosse et al Synthetic Biology 2025.02.13

Insert

GTS-mTagBFP

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pLKO.1-mTagBFP2-rMPC1 shRNA

Plasmid

#229016

Purpose

Expression of an shRNA construct that knocks down rat MPC1. This plasmid also encodes a blue fluorescent protein tag to verify transfection

Depositor

Ghazaleh Ashrafi

Article

Tiwari et al Sci Adv. 2024 Nov 15;10(46):eadp7423. do

Insert

MPC1 (Mpc1 Rat)

Use

Lentiviral

Tags

mTagBFP2

Expression

Mammalian

Mutation

Promoter

U6

Available since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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TagBFP(S2h)3(S4h)3-gs-P6f

Plasmid

#228083

Purpose

Mammalian expression of coiled coil segments (S2h)x3-(S4h)x3, with short flexible linkers, N-term TagBFP, and C-term P6f; for phase separation and forming of liquid condensates in mammalian cells.

Depositor

Roman Jerala

Article

Ramsak et al Nat Commun. 2023 Dec 2;14(1):7973. doi:

Insert

BFP, S2h, S4h, P6f

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pUC-hU6-2O-crScaffold_SV40-BFP

Plasmid

#224861

Purpose

crRNA scaffold for RfxCas13d expressed from hU6-2xTetO promoter and reporter BFP protein expressed from SV40 promoter

Depositor

Gabor Balazsi

Article

Wan et al ACS Synth Biol. 2024 Oct 18;13(10):3212-

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

hU6-2xTetO

Available since

Oct. 31, 2024

Availability

Academic Institutions and Nonprofits only

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pUC-hU6-2O-crScaffold_EF1a-BFP

Plasmid

#224862

Purpose

crRNA scaffold for RfxCas13d expressed from hU6-2xTetO promoter and reporter BFP protein expressed from EF1a promoter

Depositor

Gabor Balazsi

Article

Wan et al ACS Synth Biol. 2024 Oct 18;13(10):3212-

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

hU6-2xTetO

Available since

Oct. 31, 2024

Availability

Academic Institutions and Nonprofits only

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pUC-hU6-2O-crEGFP_EF1a-BFP

Plasmid

#224784

Purpose

EGFP targeting crRNA for RfxCas13d expressed from hU6-2xTetO promoter and non-target BFP protein expressed from EF1a promoter

Depositor

Gabor Balazsi

Article

Wan et al ACS Synth Biol. 2024 Oct 18;13(10):3212-

Insert

crEGFP

Use

CRISPR and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

hU6-2xTetO

Available since

Oct. 31, 2024

Availability

Academic Institutions and Nonprofits only

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pUC-hU6-CrEGFP_EF1a-BFP

Plasmid

#224786

Purpose

EGFP targeting crRNA for RfxCas13d expressed from hU6 promoter and non-target BFP protein expressed from EF1a promoter

Depositor

Gabor Balazsi

Article

Wan et al ACS Synth Biol. 2024 Oct 18;13(10):3212-

Insert

crBFP

Use

CRISPR and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

hU6 and hU6-2xTetO

Available since

Oct. 31, 2024

Availability

Academic Institutions and Nonprofits only

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pME_Golgi-BFP_P2A_H2A_iRFP (JDW 1007)

Plasmid

#224490

Purpose

Gateway compatible middle entry clone containing GalT-mTagBFP-HA-P2A-H2A-iRFP (BFP golgi reporter and infrared RFP nuclear reporter)

Depositor

Joshua Wythe

Article

Gillespie et al bioRxiv 2024.07.13.603267

Insert

Golgi-mTagBFP-P2A-H2A-iRFP

Use

Gateway cloning

Tags

Expression

Mutation

Promoter

Available since

Oct. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pME_Golgi-BFP_P2A_H2A_tdiRFP (JDW 1079)

Plasmid

#224491

Purpose

Gateway compatible middle entry clone containing GalT-mTagBFP-HA-P2A-H2A-tdiRFP (BFP golgi reporter and tandem infrared RFP nuclear reporter)

Depositor

Joshua Wythe

Article

Gillespie et al bioRxiv 2024.07.13.603267

Insert

Golgi-mTagBFP-P2A-H2A-tdiRFP

Use

Gateway cloning

Tags

Expression

Mutation

Promoter

Available since

Oct. 1, 2024

Availability

Academic Institutions and Nonprofits only

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p3E-V5-mTagBFP2 (JDW 871)

Plasmid

#224531

Purpose

A Gateway compatible 3' entry clone containing a V5 tagged mTagBFP2

Depositor

Joshua Wythe

Article

Gillespie et al bioRxiv 2024.07.13.603267

Insert

V5-mTagBFP2

Use

Gateway cloning

Tags

Expression

Mutation

Promoter

Available since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pME-dCas9-BFP-KRAB

Plasmid

#221502

Purpose

CRISPRi machinery fish codon optimized

Depositor

Alessandro Ori

Insert

dCas9-tagBFP-KRAB

Use

Fish expression

Tags

Expression

Mutation

D10A and H839A point mutations

Promoter

Available since

July 17, 2024

Availability

Academic Institutions and Nonprofits only

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pLKO.1 mTagBFP2 rat DJ-1 shRNA

Plasmid

#222870

Purpose

Expresses mTagBFP2 along with an shRNA against rat DJ-1

Depositor

Timothy Ryan

Article

Kokotos et al Sci Adv. 2024 Aug 23;10(34):eadn6016. do

Insert

DJ-1 shRNA (Park7 Rat)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

July 9, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO mTagBFP2-GABARAP

Plasmid

#212110

Purpose

Expression of N-terminally tagged GABARAP with mTagBFP2

Depositor

Dieter Willbold

Article

Üffing et al Autophagy Reports, 3(1). doi: 10.1080/27

Insert

mTagBFP2-GABARAP (GABARAP Human, Entacmaea quadricolor)

Use

Tags

mTagBFP2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-FLEX-H2BmTagBFP2-oG

Plasmid

#217976

Purpose

AAV transfer plasmid to express H2B-BFP and codon-optimized Rabies G protein

Depositor

Edward Callaway

Article

Patino et al Elife. 2023 Dec 14;12:e89297. doi: 10.75

Inserts

H2BmTagBFP2
oG

Use

AAV

Tags

mTAGBFP2

Expression

Mutation

Promoter

hSyn

Available since

April 22, 2024

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(CDK6-E1(6))-PGKpuro2ABFP-W

Plasmid

#200487

Purpose

Lentiviral vector expressing gRNA targeting human CDK6-E1

Depositor

Kosuke Yusa

Article

Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

Insert

CDK6-E1(6) (CDK6 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(CXCR4-E1(15))-PGKpuro2ABFP-W

Plasmid

#200478

Purpose

Lentiviral vector expressing gRNA targeting human CXCR4-E1

Depositor

Kosuke Yusa

Article

Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

Insert

CXCR4-E1(15) (CXCR4 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(CXCR4-E2(38))-PGKpuro2ABFP-W

Plasmid

#200481

Purpose

Lentiviral vector expressing gRNA targeting human CXCR4-E2

Depositor

Kosuke Yusa

Article

Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

Insert

CXCR4-E2(38) (CXCR4 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

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