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pTripz Casp1-IRES-mCherry

Plasmid

#187294

Purpose

This is Caspase1 from mouse (NM_009807.2) with its CARD domain replaced by the FKBP-V domain (dimerising domain).

Depositor

Alpha Yap

Article

Bonfim-Melo et al Cell Rep. 2022 Feb 1;38(5):110316. doi:

Insert

Caspase1 (Casp1 Mouse)

Use

Lentiviral

Tags

mCherry

Promoter

CMV

Available Since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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pTriEx-mCherry-LOV2 V416T

Plasmid

#81040

Purpose

mammalian expression of LOV V416T

Depositor

Klaus Hahn

Article

Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

Insert

LOV2 V416T

Tags

mCherry

Expression

Bacterial, Insect, and Mamm…

Mutation

V416T

Available Since

Aug. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pLVX-FLAG-mCherry-C1-centrin-1

Plasmid

#73333

Purpose

Lentiviral vector encoding centrin-1 with N-terminal FLAG and mCherry tags

Depositor

Manuel Thery

Insert

centrin-1 (CETN1 Human)

Use

Lentiviral

Tags

FLAG and mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 29, 2016

Availability

Academic Institutions and Nonprofits only

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pKLBac-Bat-FluA-trimer

Plasmid

#229858

Purpose

Influenza A/little yellow-shouldered bat/Guatemala/060/2010(H17N10) polymerase heterotrimer (self-cleaving polyprotein)

Depositor

Stephen Cusack

Article

Pflug et al Nature. 2014 Dec 18;516(7531):355-60. do

Inserts

PA_Bat-FluA_AFC35437.1
PB1_Bat-FluA_AFC35436
PB2_Bat-FluA_AFC35435.1

Expression

Insect

Available Since

Feb. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pRK5-FLAG-Sestrin2-S190W

Plasmid

#73670

Purpose

overexpression

Depositor

David Sabatini

Article

Wolfson et al Science. 2016 Jan 1;351(6268):43-8. doi:

Insert

Sesn2 (SESN2 Human)

Tags

FLAG

Expression

Mammalian

Mutation

S190W changed serine 190 to tryptophan

Promoter

CMV

Available Since

March 30, 2016

Availability

Academic Institutions and Nonprofits only
pS2-FLAG-Sestrin2-E451A

Plasmid

#73679

Purpose

overexpression

Depositor

David Sabatini

Article

Wolfson et al Science. 2016 Jan 1;351(6268):43-8. doi:

Insert

Sesn2 (SESN2 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

E451A changed Glutamate 451 to Alanine

Promoter

Sesn2

Available Since

April 12, 2016

Availability

Academic Institutions and Nonprofits only
pTRIP-SFFV-EGFP-NLS

Plasmid

#86677

Purpose

Expresses nuclear-localized GFP after lentiviral transduction. Can be used to monitor GFP leakage into the cytosol following nuclear envelope rupture events.

Depositor

Nicolas Manel

Article

Raab et al Science. 2016 Apr 15;352(6283):359-62. d

Insert

EGFP-NLS

Use

Lentiviral

Available Since

April 18, 2017

Availability

Academic Institutions and Nonprofits only

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pmax pona 12xTRICK 24xMS2SL

Plasmid

#64542

Purpose

TRICK translation biosensor with 12x copies of modified PP7 stem loops and 24x MS2 stem loops.

Depositor

Jeffrey Chao

Article

Halstead et al Science. 2015 Mar 20;347(6228):1367-671.

Insert

12x PP7 stem-loops

Tags

24xMS2 stem loops, HA, NLS, and dNP799

Expression

Mammalian

Promoter

ponA

Available Since

May 7, 2015

Availability

Academic Institutions and Nonprofits only

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TRIPZ-bioGEF-UBnc-PURO

Plasmid

#208044

Purpose

Enables inducible expression of low-affinity Avitag-UBnc; to perform bioE3; nc = non-cleavable mutation; selection with puromycin

Depositor

Rosa Barrio, James Sutherland

Article

Barroso-Gomila et al Nat Commun. 2023 Nov 23;14(1):7656. doi:

Insert

UBnc (UBC Human)

Use

Lentiviral

Tags

Low-affinity Avitag

Mutation

L73P Mutation near C-terminus suppresses cleavage…

Promoter

tetO/UBC

Available Since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only

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TRIPZ-bioGEF-UBwt-PURO

Plasmid

#208045

Purpose

Enables inducible expression of low-affinity Avitag-Ubwt; to perform bioE3; selection with puromycin

Depositor

Rosa Barrio, James Sutherland

Article

Barroso-Gomila et al Nat Commun. 2023 Nov 23;14(1):7656. doi:

Insert

Ub (UBC Human)

Use

Lentiviral

Tags

Low-affinity Avitag

Promoter

tetO/UBC

Available Since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pLVX puro TRIM21-GFP

Plasmid

#116941

Purpose

Expression of TRIM21-GFP fusion protein

Depositor

Gaudenz Danuser

Article

Park et al Nature. 2020 Feb 12. pii: 10.1038/s41586

Insert

TRIM21 - GFP (TRIM21 Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Available Since

Feb. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pTRIP-SFFV-mtagBFP-2A STING

Plasmid

#102586

Purpose

Lentivector to express STING and mTagBFP

Depositor

Nicolas Manel

Article

Cerboni et al J Exp Med. 2017 Jun 5;214(6):1769-1785.

Insert

STING (STING1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

SFFV

Available Since

Jan. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pTriEx-NTOM20-mVenus-Zdk2

Plasmid

#81011

Purpose

Expression of Zdk2 anchored to mitochondria

Depositor

Klaus Hahn

Article

Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

Insert

Zdk2

Tags

6x His tag, FLAG, NTOM20, and mVenus

Expression

Bacterial, Insect, and Mamm…

Available Since

Aug. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pRK5-FLAG-Sestrin1 isoform1

Plasmid

#72594

Purpose

overexpression, codon optimized for mammalian expression

Depositor

David Sabatini

Article

Chantranupong et al Cell Rep. 2014 Oct 9;9(1):1-8. doi: 10.1

Insert

SESN1 (SESN1 Human)

Tags

FLAG

Expression

Mammalian

Mutation

codon optimized for mammalian expression, see dep…

Promoter

CMV

Available Since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only
pTRIP-SFFV-mtagBFP-2A RIGI WT

Plasmid

#167289

Purpose

Lentiviral expression construct encoding a TagBFP in frame with a self-cleaving wild-type RLR sensor RIG-I

Depositor

Agnel Sfeir

Article

Tigano et al Nature. 2021 Mar;591(7850):477-481. doi:

Insert

DDX58 (RIGI Human)

Use

Lentiviral

Tags

tagBFP

Expression

Mammalian

Promoter

SFFV

Available Since

April 21, 2021

Availability

Academic Institutions and Nonprofits only

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pMSCV-IRES-mAmetrine 1.1 FP

Plasmid

#52113

Purpose

a bicistronic IRES-mAtetrine containing retroviral vector

Depositor

Dario Vignali

Type

Empty backbone

Use

Retroviral

Tags

IRES-mAmetrine 1.1

Expression

Mammalian

Available Since

March 27, 2014

Availability

Academic Institutions and Nonprofits only

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pEGFP Centrin2 (Nigg UK185)

Plasmid

#41147

Depositor

Erich Nigg

Article

Kleylein-Sohn et al Dev Cell. 2007 Aug;13(2):190-202.

Insert

Centrin-2 (CETN2 Human)

Tags

eGFP

Expression

Mammalian

Promoter

CMV

Available Since

June 7, 2013

Availability

Academic Institutions and Nonprofits only

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pTRIPZ-2xFLAG-2xSTREP-E2F1

Plasmid

#236433

Purpose

doxycycline inducible expression of E2F1 in mammalian cells

Depositor

Michele Pagano

Article

Clijsters et al Mol Cell. 2019 Jun 20;74(6):1264-1277.e7

Insert

E2F1 (E2F1 Human)

Tags

FLAG-FLAG-STREP-STREP

Expression

Mammalian

Available Since

May 28, 2025

Availability

Academic Institutions and Nonprofits only

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katushka-FMDV-P2A-pTRIP

Plasmid

#163522

Purpose

Used to construct lentivirus to express katushka in mammalian cells with option for a second insert

Depositor

Thomas Weber

Insert

stopless Katushka-P2A

Use

Lentiviral

Expression

Bacterial and Mammalian

Available Since

Jan. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pTriEx-RhoA FLARE.sc Biosensor WT

Plasmid

#12150

Depositor

Klaus Hahn

Article

Pertz et al Nature. 2006 Apr 20. 440(7087):1069-72.

Insert

RhoA Biosensor (RHOA Human)

Tags

6xHis

Expression

Bacterial, Insect, and Mamm…

Available Since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

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